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Entry version 158 (23 Feb 2022)
Sequence version 1 (01 May 1997)
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Protein

Rho guanine nucleotide exchange factor 28

Gene

Arhgef28

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Functions as a RHOA-specific guanine nucleotide exchange factor regulating signaling pathways downstream of integrins and growth factor receptors. Functions in axonal branching, synapse formation and dendritic morphogenesis. Functions also in focal adhesion formation, cell motility and B-lymphocytes activation. May regulate NEFL expression and aggregation and play a role in apoptosis.

10 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section specifies the position(s) and type(s) of zinc fingers within the protein.<p><a href='/help/zn_fing' target='_top'>More...</a></p>Zinc fingeri651 – 698Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST48

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionGuanine-nucleotide releasing factor, RNA-binding
Biological processDifferentiation
LigandMetal-binding, Zinc

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-MMU-3928662, EPHB-mediated forward signaling
R-MMU-8980692, RHOA GTPase cycle
R-MMU-9013026, RHOB GTPase cycle
R-MMU-9013106, RHOC GTPase cycle

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Rho guanine nucleotide exchange factor 28
Alternative name(s):
190 kDa guanine nucleotide exchange factor
Short name:
p190-RhoGEF
Short name:
p190RhoGEF
Rho guanine nucleotide exchange factor
Rho-interacting protein 2
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Arhgef28
Synonyms:Kiaa1998, Rgnef, Rhoip2, Rip2
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMus musculus (Mouse)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10090 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000589 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Unplaced

Organism-specific databases

Mouse genome database (MGD) from Mouse Genome Informatics (MGI)

More...
MGIi
MGI:1346016, Arhgef28

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi1003Y → A: Loss of function. 1 Publication1
Mutagenesisi1375N → A: Alters interaction with YWHAH. 1 Publication1
Mutagenesisi1376L → A: Alters interaction with YWHAH. 1 Publication1
Mutagenesisi1563F → A: Alters interaction with MAPK8IP1. 1 Publication1
Mutagenesisi1565N → A: Alters interaction with MAPK8IP1. 1 Publication1
Mutagenesisi1568F → A: Alters interaction with MAPK8IP1. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000809671 – 1693Rho guanine nucleotide exchange factor 28Add BLAST1693

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei312PhosphoserineBy similarity1
Modified residuei314PhosphoserineBy similarity1
Modified residuei478PhosphoserineBy similarity1
Modified residuei623PhosphoserineBy similarity1
Modified residuei1535PhosphoserineBy similarity1
Modified residuei1604PhosphoserineCombined sources1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Phosphorylated on tyrosine upon stimulation of cells by laminin.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
P97433

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P97433

PRoteomics IDEntifications database

More...
PRIDEi
P97433

ProteomicsDB: a multi-organism proteome resource

More...
ProteomicsDBi
283256 [P97433-1]
283257 [P97433-2]

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
P97433

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...
PhosphoSitePlusi
P97433

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Highly enriched in the brain (at protein level). Also detected in lung and kidney.2 Publications

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Up-regulated in B-lymphocytes upon CD40 stimulation. Up-regulated by PTK2B/PYK2 (at protein level).2 Publications

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homooligomer; forms some cytoplasmic aggregates.

Forms a complex with MAPK8 and MAPK8IP1.

Interacts with RHOA.

Interacts with microtubules.

Interacts with YWHAE and YWHAH.

Interacts with PTK2/FAK1.

Interacts with NEFL.

Interacts with CTNND2; prevents interaction with RHOA.

7 Publications

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
225739, 11 interactors

Protein interaction database and analysis system

More...
IntActi
P97433, 2 interactors

Molecular INTeraction database

More...
MINTi
P97433

STRING: functional protein association networks

More...
STRINGi
10090.ENSMUSP00000105053

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
P97433, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P97433

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini846 – 1041DHPROSITE-ProRule annotationAdd BLAST196
Domaini1095 – 1184PHPROSITE-ProRule annotationAdd BLAST90

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni288 – 337DisorderedSequence analysisAdd BLAST50
Regioni476 – 532DisorderedSequence analysisAdd BLAST57
Regioni1184 – 1205DisorderedSequence analysisAdd BLAST22
Regioni1289 – 1328DisorderedSequence analysisAdd BLAST40
Regioni1292 – 1301Interaction with PTK2/FAK1; required for regulation of axonal branching and synapse formation10
Regioni1369 – 1380Mediates cytoplasmic retention and interaction with YWHAH1 PublicationAdd BLAST12
Regioni1421 – 1693Interaction with microtubulesAdd BLAST273
Regioni1493 – 1524RNA-bindingBy similarityAdd BLAST32
Regioni1563 – 1576Mediates cytoplasmic retention and interaction with MAPK8IP11 PublicationAdd BLAST14
Regioni1602 – 1693DisorderedSequence analysisAdd BLAST92

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and domains' section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili1421 – 1522Sequence analysisAdd BLAST102

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi301 – 337Basic and acidic residuesSequence analysisAdd BLAST37
Compositional biasi498 – 532Polar residuesSequence analysisAdd BLAST35
Compositional biasi1190 – 1205Basic and acidic residuesSequence analysisAdd BLAST16
Compositional biasi1289 – 1325Polar residuesSequence analysisAdd BLAST37
Compositional biasi1617 – 1673Polar residuesSequence analysisAdd BLAST57
Compositional biasi1674 – 1693Basic and acidic residuesSequence analysisAdd BLAST20

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri651 – 698Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST48

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG3519, Eukaryota
KOG3520, Eukaryota

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P97433

Database of Orthologous Groups

More...
OrthoDBi
69816at2759

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P97433

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00029, C1, 1 hit
cd14680, PH_p190RhoGEF, 1 hit
cd00160, RhoGEF, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.20.900.10, 1 hit
2.30.29.30, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR037819, ARHGEF28_PH
IPR035899, DBL_dom_sf
IPR000219, DH-domain
IPR002219, PE/DAG-bd
IPR011993, PH-like_dom_sf
IPR041020, PH_16
IPR001849, PH_domain

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00130, C1_1, 1 hit
PF17838, PH_16, 1 hit
PF00621, RhoGEF, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00109, C1, 1 hit
SM00233, PH, 1 hit
SM00325, RhoGEF, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF48065, SSF48065, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50010, DH_2, 1 hit
PS50003, PH_DOMAIN, 1 hit
PS00479, ZF_DAG_PE_1, 1 hit
PS50081, ZF_DAG_PE_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P97433-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <p><strong>What is the canonical sequence?</strong><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MELSCSEVPL YGQKTVYAKF GKNVYLPEDA EFYFVYGGSH QRHVVIADRV
60 70 80 90 100
QDNVLQSSIP GHWLQETVTV SVCLCSEGYS PVTMGSGSVT YVDNMACRLA
110 120 130 140 150
RLLVTQADRL TACSHQTLLT PFALTVEALP ALDEELVLAL TQLELPLGWT
160 170 180 190 200
VLGNSSLEVS LHRESLLHLA VRWALPKLFH FLLCLPGGVK ALKLPNEEAT
210 220 230 240 250
TPLDLALQGG HSTLVEDITN FQGSHSPGFS RLRLNEEATL QFVHSSETLT
260 270 280 290 300
LTVNHTAEHL LEADIKLFRK YFWDRAFLVK ALEQEAKTEK ATMPSGAAET
310 320 330 340 350
EEEVRNLESG RSPSEEEEDA KSIKSQVDGP SEHEDQDRLA LDRSFDGLKK
360 370 380 390 400
SKHVPASLAA GQLSDVLNGG DEVYANCMVI DQVGDLDINY INLEGLSTHT
410 420 430 440 450
SPESGRSMLG PQACMHTLPP DTSPCGRPLI ENSEGTLDAA ASQSFVTPSS
460 470 480 490 500
SRTSNLNLSF GLHGFEKEQS HLKKRSSSLD ALVADSEGEG GSEPPICYAV
510 520 530 540 550
GSQSSPRTGL PSGDELDSFE TNTEPDCNIS RTESLSLSST LHSKESLLSG
560 570 580 590 600
IRSRSYSCSS PKISSGKSRL VRDFTVCSTS EEQRSYSFQE PPGEKRIQEE
610 620 630 640 650
EWDEYVIPAK SESEKYKVSR TFSFLMNRMT SPRNKSKMKN KDTKEKEKMN
660 670 680 690 700
RHQFVPGTFS GVLQCSGCDK TLLGKESLQC ANCKANTHKG CKDAVPPCTK
710 720 730 740 750
KFQEKYNKNK PQSILGSSSV RDVPAPGLSL HPSSSMPIGL PAGRKEFAAQ
760 770 780 790 800
VHPLSRSVPG TTLESFRRAV TSLESEGDSW RSRSHSDELF QSMGSSPSTE
810 820 830 840 850
SFMMEDVVDS SLWIDLSSDA QEFEAESWSL VVDPSFCSRQ EKDVIKRQDV
860 870 880 890 900
IFELMQTEVH HIQTLLIMSE VFRKGMKEEL QLDHSTVDKI FPCLDELLET
910 920 930 940 950
HRHFFFSMKE RRQESCAGSD RNFVINQIGD ILVQQFSEEN ASKMKRIYGE
960 970 980 990 1000
FCSHHKEAMS LFKELQQNKK FQNFIKIRNS NLLARRRGIP ECILLVTQRI
1010 1020 1030 1040 1050
TKYPVLVERI LQYTKERTEE HRDLCKALGL IKDMIAAVDL KVSEYEKNQK
1060 1070 1080 1090 1100
WLEILNKIEN KTYTKLKNGH VFRKQALLSQ ERALLHDGLV YWKTATGRFK
1110 1120 1130 1140 1150
DILALLLTDV LLFLQEKDQK YIFAAVDQKP SVISLQKLIA REVANEERGM
1160 1170 1180 1190 1200
FLISASSAGP EMYEIHTNSK EERNNWMRRI QQAVESCPEE EGGRTSESDE
1210 1220 1230 1240 1250
ERRKAEARVA KIQQCQEILS NQDQQICTYL EEKLHIYAEL GELSGFEDVH
1260 1270 1280 1290 1300
LEPHLLIKPD PGEPPQAASL LAAALREAES LQVAVKASKM GDVSQSSEES
1310 1320 1330 1340 1350
PGGTVLMDTP STQDVPASPT ASLVTEGTEG RGCWDVDPGL QGVVTDLAVS
1360 1370 1380 1390 1400
DAGEKVEYRS FSGSSQSEII QAIQNLTRLL YSLQAALTIQ DSHIEIHKLV
1410 1420 1430 1440 1450
LQQRESLAPS HSFRGGPLQD QEKSRYLEKQ REELANIHKL QHQFQQEQRR
1460 1470 1480 1490 1500
WHRTCDQQQR EQEAQESWLQ ARERECQSQE ELLLRHRSEL DHQLQEYQQS
1510 1520 1530 1540 1550
LERLREGQRM VERERQKMRV QQGLLGHCKH SRQRSLPAVF SPGSKEVTEL
1560 1570 1580 1590 1600
NRAESLCHEN SFFINEAFGH MSLNTSNKPN PSGVPWDAHP LEGSHFDLAR
1610 1620 1630 1640 1650
TSESPTELKI DISQPPDVNS ELWTTGPGHQ RPALQENSKE SYKNVADLDS
1660 1670 1680 1690
FQSESSSPQD SNQRWPPATD THNRSKTKSS DGGWTRWRCW RRG
Length:1,693
Mass (Da):190,326
Last modified:May 1, 1997 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i4E96087C449FF14C
GO
Isoform 2 (identifier: P97433-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1322-1324: SLV → CGI
     1325-1693: Missing.

Show »
Length:1,324
Mass (Da):147,757
Checksum:iB86E628B11ED16A3
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G5E8P2G5E8P2_MOUSE
Rho guanine nucleotide exchange fac...
Arhgef28 Rgnef
1,700Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A571BEH9A0A571BEH9_MOUSE
Rho guanine nucleotide exchange fac...
Arhgef28
1,387Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A286YDI4A0A286YDI4_MOUSE
Rho guanine nucleotide exchange fac...
Arhgef28
1,324Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A286YCY0A0A286YCY0_MOUSE
Rho guanine nucleotide exchange fac...
Arhgef28
643Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A286YD71A0A286YD71_MOUSE
Rho guanine nucleotide exchange fac...
Arhgef28
11Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAH22978 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH75734 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence BAD32603 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti340A → P in AAH75734 (PubMed:15489334).Curated1
Sequence conflicti481A → T in AAH75734 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0321451322 – 1324SLV → CGI in isoform 2. 1 Publication3
Alternative sequenceiVSP_0321461325 – 1693Missing in isoform 2. 1 PublicationAdd BLAST369

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U73199 mRNA Translation: AAB18197.1
AK173325 mRNA Translation: BAD32603.1 Different initiation.
BC022978 mRNA Translation: AAH22978.1 Sequence problems.
BC075734 mRNA Translation: AAH75734.1 Sequence problems.

Protein sequence database of the Protein Information Resource

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PIRi
T30867

NCBI Reference Sequences

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RefSeqi
NP_036156.2, NM_012026.2

Genome annotation databases

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
110596

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
mmu:110596

UCSC genome browser

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UCSCi
uc007rol.1, mouse [P97433-2]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73199 mRNA Translation: AAB18197.1
AK173325 mRNA Translation: BAD32603.1 Different initiation.
BC022978 mRNA Translation: AAH22978.1 Sequence problems.
BC075734 mRNA Translation: AAH75734.1 Sequence problems.
PIRiT30867
RefSeqiNP_036156.2, NM_012026.2

3D structure databases

SMRiP97433
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi225739, 11 interactors
IntActiP97433, 2 interactors
MINTiP97433
STRINGi10090.ENSMUSP00000105053

PTM databases

iPTMnetiP97433
PhosphoSitePlusiP97433

Proteomic databases

MaxQBiP97433
PaxDbiP97433
PRIDEiP97433
ProteomicsDBi283256 [P97433-1]
283257 [P97433-2]

Protocols and materials databases

The DNASU plasmid repository

More...
DNASUi
110596

Genome annotation databases

GeneIDi110596
KEGGimmu:110596
UCSCiuc007rol.1, mouse [P97433-2]

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
64283
MGIiMGI:1346016, Arhgef28

Rodent Unidentified Gene-Encoded large proteins database

More...
Rougei
Search...

Phylogenomic databases

eggNOGiKOG3519, Eukaryota
KOG3520, Eukaryota
InParanoidiP97433
OrthoDBi69816at2759
PhylomeDBiP97433

Enzyme and pathway databases

ReactomeiR-MMU-3928662, EPHB-mediated forward signaling
R-MMU-8980692, RHOA GTPase cycle
R-MMU-9013026, RHOB GTPase cycle
R-MMU-9013106, RHOC GTPase cycle

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...
BioGRID-ORCSi
110596, 0 hits in 63 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

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ChiTaRSi
Arhgef28, mouse

Protein Ontology

More...
PROi
PR:P97433
RNActiP97433, protein

The Stanford Online Universal Resource for Clones and ESTs

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SOURCEi
Search...

Family and domain databases

CDDicd00029, C1, 1 hit
cd14680, PH_p190RhoGEF, 1 hit
cd00160, RhoGEF, 1 hit
Gene3Di1.20.900.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR037819, ARHGEF28_PH
IPR035899, DBL_dom_sf
IPR000219, DH-domain
IPR002219, PE/DAG-bd
IPR011993, PH-like_dom_sf
IPR041020, PH_16
IPR001849, PH_domain
PfamiView protein in Pfam
PF00130, C1_1, 1 hit
PF17838, PH_16, 1 hit
PF00621, RhoGEF, 1 hit
SMARTiView protein in SMART
SM00109, C1, 1 hit
SM00233, PH, 1 hit
SM00325, RhoGEF, 1 hit
SUPFAMiSSF48065, SSF48065, 1 hit
PROSITEiView protein in PROSITE
PS50010, DH_2, 1 hit
PS50003, PH_DOMAIN, 1 hit
PS00479, ZF_DAG_PE_1, 1 hit
PS50081, ZF_DAG_PE_2, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiARG28_MOUSE
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P97433
Secondary accession number(s): A0PJC1, Q69Z41, Q6DI55
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1997
Last modified: February 23, 2022
This is version 158 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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