UniProtKB - P97414 (KCNQ1_MOUSE)
Potassium voltage-gated channel subfamily KQT member 1
Kcnq1
Functioni
GO - Molecular functioni
- calmodulin binding Source: MGI
- delayed rectifier potassium channel activity Source: UniProtKB
- identical protein binding Source: MGI
- ion channel binding Source: MGI
- outward rectifier potassium channel activity Source: UniProtKB
- phosphatidylinositol-4,5-bisphosphate binding Source: UniProtKB
- potassium channel activity Source: MGI
- protein kinase A catalytic subunit binding Source: MGI
- protein kinase A regulatory subunit binding Source: MGI
- protein phosphatase 1 binding Source: MGI
- scaffold protein binding Source: MGI
- voltage-gated potassium channel activity Source: UniProtKB
- voltage-gated potassium channel activity involved in atrial cardiac muscle cell action potential repolarization Source: MGI
- voltage-gated potassium channel activity involved in cardiac muscle cell action potential repolarization Source: MGI
- voltage-gated potassium channel activity involved in ventricular cardiac muscle cell action potential repolarization Source: MGI
GO - Biological processi
- atrial cardiac muscle cell action potential Source: MGI
- cardiac muscle contraction Source: MGI
- cellular response to cAMP Source: MGI
- cellular response to drug Source: MGI
- circulatory system development Source: MGI
- gene silencing Source: MGI
- inner ear development Source: UniProtKB
- intestinal absorption Source: UniProtKB
- membrane repolarization Source: UniProtKB
- membrane repolarization during action potential Source: MGI
- membrane repolarization during atrial cardiac muscle cell action potential Source: MGI
- membrane repolarization during cardiac muscle cell action potential Source: MGI
- membrane repolarization during ventricular cardiac muscle cell action potential Source: MGI
- negative regulation of delayed rectifier potassium channel activity Source: MGI
- negative regulation of insulin secretion Source: MGI
- negative regulation of voltage-gated potassium channel activity Source: MGI
- positive regulation of cardiac muscle contraction Source: MGI
- positive regulation of gastric acid secretion Source: MGI
- positive regulation of heart rate Source: MGI
- positive regulation of potassium ion transmembrane transport Source: MGI
- potassium ion export across plasma membrane Source: MGI
- potassium ion transmembrane transport Source: MGI
- potassium ion transport Source: MGI
- regulation of atrial cardiac muscle cell membrane repolarization Source: MGI
- regulation of gastric acid secretion Source: UniProtKB
- regulation of gene expression by genetic imprinting Source: MGI
- regulation of heart rate by cardiac conduction Source: MGI
- regulation of membrane potential Source: MGI
- regulation of membrane repolarization Source: MGI
- regulation of ventricular cardiac muscle cell membrane repolarization Source: MGI
- renal absorption Source: UniProtKB
- response to anesthetic Source: MGI
- ventricular cardiac muscle cell action potential Source: MGI
Keywordsi
Molecular function | Calmodulin-binding, Ion channel, Potassium channel, Voltage-gated channel |
Biological process | Ion transport, Potassium transport, Transport |
Ligand | Potassium |
Enzyme and pathway databases
Reactomei | R-MMU-1296072, Voltage gated Potassium channels R-MMU-5576890, Phase 3 - rapid repolarisation R-MMU-5576893, Phase 2 - plateau phase |
Protein family/group databases
TCDBi | 1.A.1.15.1, the voltage-gated ion channel (vic) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Potassium voltage-gated channel subfamily KQT member 1Alternative name(s): IKs producing slow voltage-gated potassium channel subunit alpha KvLQT11 Publication KQT-like 1By similarity Voltage-gated potassium channel subunit Kv7.1By similarity |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:108083, Kcnq1 |
Subcellular locationi
Endosome
- Early endosome By similarity
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein By similarity
- Basolateral cell membrane By similarity
Endoplasmic reticulum
- Endoplasmic reticulum By similarity
Other locations
- Cytoplasmic vesicle membrane By similarity
- Membrane raft By similarity
Note: Colocalized with KCNE3 at the plasma membrane. Upon 17beta-oestradiol treatment, colocalizes with RAB5A at early endosome. Heterotetramer with KCNQ5 is highly retained at the endoplasmic reticulum and is localized outside of lipid raft microdomains. During the early stages of epithelial cell polarization induced by the calcium switch it removed from plasma membrane to the endoplasmic reticulum where it retained and it is redistributed to the basolateral cell surface in a PI3K-dependent manner at a later stage.By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
Endosome
- early endosome Source: MGI
- late endosome Source: MGI
Lysosome
- lysosome Source: MGI
Plasma Membrane
- basolateral plasma membrane Source: UniProtKB
- integral component of plasma membrane Source: MGI
- plasma membrane Source: UniProtKB
- sarcolemma Source: MGI
- voltage-gated potassium channel complex Source: MGI
Other locations
- cytoplasm Source: UniProtKB
- cytoplasmic vesicle membrane Source: MGI
- integral component of membrane Source: GO_Central
- ion channel complex Source: UniProtKB
- membrane raft Source: UniProtKB
- zymogen granule membrane Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 120 | CytoplasmicSequence analysisAdd BLAST | 120 | |
Transmembranei | 121 – 141 | Helical; Name=Segment S1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 142 – 146 | ExtracellularSequence analysis | 5 | |
Transmembranei | 147 – 167 | Helical; Name=Segment S2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 168 – 195 | CytoplasmicSequence analysisAdd BLAST | 28 | |
Transmembranei | 196 – 216 | Helical; Name=Segment S3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 217 – 224 | ExtracellularSequence analysis | 8 | |
Transmembranei | 225 – 247 | Helical; Voltage-sensor; Name=Segment S4Sequence analysisAdd BLAST | 23 | |
Topological domaini | 248 – 260 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 261 – 281 | Helical; Name=Segment S5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 282 – 298 | ExtracellularSequence analysisAdd BLAST | 17 | |
Intramembranei | 299 – 319 | Pore-forming; Name=Segment H5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 320 – 330 | ExtracellularSequence analysisAdd BLAST | 11 | |
Transmembranei | 331 – 351 | Helical; Name=Segment S6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 352 – 668 | CytoplasmicSequence analysisAdd BLAST | 317 |
Keywords - Cellular componenti
Cell membrane, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, MembranePathology & Biotechi
Disruption phenotypei
Chemistry databases
DrugCentrali | P97414 |
GuidetoPHARMACOLOGYi | 560 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000054024 | 1 – 668 | Potassium voltage-gated channel subfamily KQT member 1Add BLAST | 668 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 27 | Phosphoserine; by PKABy similarity | 1 | |
Glycosylationi | 288 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 406 | PhosphoserineCombined sources | 1 | |
Modified residuei | 408 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Glycoprotein, Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | P97414 |
PaxDbi | P97414 |
PeptideAtlasi | P97414 |
PRIDEi | P97414 |
PTM databases
GlyGeni | P97414, 1 site |
iPTMneti | P97414 |
PhosphoSitePlusi | P97414 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000009545, Expressed in stria vascularis of cochlear duct and 168 other tissues |
Genevisiblei | P97414, MM |
Interactioni
Subunit structurei
Tetramer (By similarity). Heterotetramer with KCNE1; form the native cardiac channel I(Ks) which increases the amplitude and slows down the activation kinetics of outward potassium current and targets to the membrane raft (By similarity) (PubMed:8900282).
Interacts (via C-terminus) with CALM; forms a heterooctameric structure (with 4:4 KCNQ1:CALM stoichiometry) in a calcium-independent manner.
Interacts with AKAP9; targets protein kinase A (PKA) catalytic and regulatory subunits and protein phosphatase 1 (PP1) to the KCNQ1-KCNE1 complex, allowing PKA-mediated phosphorylation and increase of delayed rectifier potassium channel activity.
Interacts with KCNE2; form an heterooligomer complex that targets to the membrane raft and leading to currents with an apparently instantaneous activation, a rapid deactivation process and a linear current-voltage relationship and decreases the amplitude of the outward current.
Interacts with AP2M1; mediates estrogen-induced internalization via clathrin-coated vesicles.
Interacts with NEDD4L; promotes internalization and decreases I(Ks) currents.
Interacts with USP2; counteracts the NEDD4L-specific down-regulation of I(Ks) and restore plasma membrane localization. Heterotetramer with KCNQ5; has a voltage-gated potassium channel activity.
Interacts with KCNE3; alters membrane raft localization.
Interacts with KCNE4; impairs KCNQ1 localization in lipid rafts and inhibits voltage-gated potassium channel activity.
Interacts with KCNE5; impairs KCNQ1 localization in lipid rafts and only conducts current upon strong and continued depolarization (By similarity).
By similarity1 PublicationGO - Molecular functioni
- calmodulin binding Source: MGI
- identical protein binding Source: MGI
- ion channel binding Source: MGI
- protein kinase A catalytic subunit binding Source: MGI
- protein kinase A regulatory subunit binding Source: MGI
- protein phosphatase 1 binding Source: MGI
- scaffold protein binding Source: MGI
Protein-protein interaction databases
ComplexPortali | CPX-3274, KCNQ1-KCNE1 I(Ks) channel complex CPX-5826, Kv7.1 channel complex |
IntActi | P97414, 117 interactors |
STRINGi | 10090.ENSMUSP00000009689 |
Miscellaneous databases
RNActi | P97414, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 369 – 381 | Interaction with CALMBy similarityAdd BLAST | 13 | |
Regioni | 514 – 528 | Interaction with CALM; calcium-dependentBy similarityAdd BLAST | 15 | |
Regioni | 534 – 571 | Interaction with KCNE1 C-terminusBy similarityAdd BLAST | 38 | |
Regioni | 587 – 615 | Interaction with AKAP9By similarityAdd BLAST | 29 | |
Regioni | 588 – 619 | C-terminal assembly domainBy similarityAdd BLAST | 32 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 584 – 620 | By similarityAdd BLAST | 37 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 311 – 316 | Selectivity filterBy similarity | 6 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coil, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1419, Eukaryota |
GeneTreei | ENSGT00940000161001 |
InParanoidi | P97414 |
OMAi | RDHHRAT |
OrthoDBi | 1168835at2759 |
TreeFami | TF315186 |
Family and domain databases
InterProi | View protein in InterPro IPR005821, Ion_trans_dom IPR003937, K_chnl_volt-dep_KCNQ IPR013821, K_chnl_volt-dep_KCNQ_C IPR005827, K_chnl_volt-dep_KCQN1 IPR028325, VG_K_chnl |
PANTHERi | PTHR11537, PTHR11537, 1 hit PTHR11537:SF144, PTHR11537:SF144, 1 hit |
Pfami | View protein in Pfam PF00520, Ion_trans, 1 hit PF03520, KCNQ_channel, 1 hit |
PRINTSi | PR01460, KCNQ1CHANNEL PR01459, KCNQCHANNEL |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDTASSPPSA ERKRAGWSRL LGARRGSAVV KKCPFSLELA EGGPEGSTVY
60 70 80 90 100
APIAPTGAPG LAPPMSTPVS PAPAPADLGP RPRVSLDPRV SIYSARRPLL
110 120 130 140 150
ARTHIQGRVY NFLERPTGWK CFVYHFTVFL IVLVCLIFSV LSTIEQYAAL
160 170 180 190 200
ATGTLFWMEI VLVVFFGTEY VVRLWSAGCR SKYVGIWGRL RFARKPISII
210 220 230 240 250
DLIVVVASMV VLCVGSKGQV FATSAIRGIR FLQILRMLHV DRQGGTWRLL
260 270 280 290 300
GSVVFIHRQE LITTLYIGFL GLIFSSYFVY LAEKDAVNES GRIEFGSYAD
310 320 330 340 350
ALWWGVVTVT TIGYGDKVPQ TWVGKTIASC FSVFAISFFA LPAGILGSGF
360 370 380 390 400
ALKVQQKQRQ KHFNRQIPAA ASLIQTAWRC YAAENPDSAT WKIYVRKPAR
410 420 430 440 450
SHTLLSPSPK PKKSVMVKKK KFKLDKDNGM SPGEKMFNVP HITYDPPEDR
460 470 480 490 500
RPDHFSIDGY DSSVRKSPTL LEVSTPHFLR TNSFAEDLDL EGETLLTPIT
510 520 530 540 550
HVSQLRDHHR ATIKVIRRMQ YFVAKKKFQQ ARKPYDVRDV IEQYSQGHLN
560 570 580 590 600
LMVRIKELQR RLDQSIGKPS LFIPISEKSK DRGSNTIGAR LNRVEDKVTQ
610 620 630 640 650
LDQRLVIITD MLHQLLSMQQ GGPTCNSRSQ VVASNEGGSI NPELFLPSNS
660
LPTYEQLTVP QTGPDEGS
The sequence of this isoform differs from the canonical sequence as follows:
1-111: Missing.
112-128: FLERPTGWKCFVYHFTV → MHQLPTSILIPKNMPGG
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A087WNY7 | A0A087WNY7_MOUSE | IKs producing slow voltage-gated po... | Kcnq1 | 440 | Annotation score: | ||
A0A087WRK7 | A0A087WRK7_MOUSE | Potassium voltage-gated channel sub... | Kcnq1 | 60 | Annotation score: | ||
A0A087WPJ2 | A0A087WPJ2_MOUSE | Potassium voltage-gated channel sub... | Kcnq1 | 49 | Annotation score: | ||
A0A087WRU8 | A0A087WRU8_MOUSE | Potassium voltage-gated channel sub... | Kcnq1 | 30 | Annotation score: | ||
A0A087WPA8 | A0A087WPA8_MOUSE | Potassium voltage-gated channel sub... | Kcnq1 | 39 | Annotation score: | ||
A0A087WQE3 | A0A087WQE3_MOUSE | Potassium voltage-gated channel sub... | Kcnq1 | 77 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 473 | V → L in AAP93874 (PubMed:15004216).Curated | 1 | |
Sequence conflicti | 473 | V → L in AAB36518 (PubMed:8900282).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_011748 | 1 – 111 | Missing in isoform II. CuratedAdd BLAST | 111 | |
Alternative sequenceiVSP_000983 | 112 – 128 | FLERP…YHFTV → MHQLPTSILIPKNMPGG in isoform II. CuratedAdd BLAST | 17 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY331142 mRNA Translation: AAP93874.1 AK154244 mRNA Translation: BAE32460.1 BC045142 mRNA Translation: AAH45142.2 BC055304 mRNA Translation: AAH55304.1 U70068 mRNA Translation: AAB36518.1 Different initiation. AJ002201 mRNA Translation: CAA05246.1 |
CCDSi | CCDS22039.1 [P97414-1] |
RefSeqi | NP_032460.2, NM_008434.2 [P97414-1] |
Genome annotation databases
Ensembli | ENSMUST00000009689; ENSMUSP00000009689; ENSMUSG00000009545 [P97414-1] |
GeneIDi | 16535 |
KEGGi | mmu:16535 |
UCSCi | uc009kpb.1, mouse [P97414-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY331142 mRNA Translation: AAP93874.1 AK154244 mRNA Translation: BAE32460.1 BC045142 mRNA Translation: AAH45142.2 BC055304 mRNA Translation: AAH55304.1 U70068 mRNA Translation: AAB36518.1 Different initiation. AJ002201 mRNA Translation: CAA05246.1 |
CCDSi | CCDS22039.1 [P97414-1] |
RefSeqi | NP_032460.2, NM_008434.2 [P97414-1] |
3D structure databases
BMRBi | P97414 |
SMRi | P97414 |
ModBasei | Search... |
Protein-protein interaction databases
ComplexPortali | CPX-3274, KCNQ1-KCNE1 I(Ks) channel complex CPX-5826, Kv7.1 channel complex |
IntActi | P97414, 117 interactors |
STRINGi | 10090.ENSMUSP00000009689 |
Chemistry databases
DrugCentrali | P97414 |
GuidetoPHARMACOLOGYi | 560 |
Protein family/group databases
TCDBi | 1.A.1.15.1, the voltage-gated ion channel (vic) superfamily |
PTM databases
GlyGeni | P97414, 1 site |
iPTMneti | P97414 |
PhosphoSitePlusi | P97414 |
Proteomic databases
MaxQBi | P97414 |
PaxDbi | P97414 |
PeptideAtlasi | P97414 |
PRIDEi | P97414 |
Protocols and materials databases
Antibodypediai | 4357, 467 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000009689; ENSMUSP00000009689; ENSMUSG00000009545 [P97414-1] |
GeneIDi | 16535 |
KEGGi | mmu:16535 |
UCSCi | uc009kpb.1, mouse [P97414-1] |
Organism-specific databases
CTDi | 3784 |
MGIi | MGI:108083, Kcnq1 |
Phylogenomic databases
eggNOGi | KOG1419, Eukaryota |
GeneTreei | ENSGT00940000161001 |
InParanoidi | P97414 |
OMAi | RDHHRAT |
OrthoDBi | 1168835at2759 |
TreeFami | TF315186 |
Enzyme and pathway databases
Reactomei | R-MMU-1296072, Voltage gated Potassium channels R-MMU-5576890, Phase 3 - rapid repolarisation R-MMU-5576893, Phase 2 - plateau phase |
Miscellaneous databases
BioGRID-ORCSi | 16535, 1 hit in 17 CRISPR screens |
ChiTaRSi | Kcnq1, mouse |
PROi | PR:P97414 |
RNActi | P97414, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000009545, Expressed in stria vascularis of cochlear duct and 168 other tissues |
Genevisiblei | P97414, MM |
Family and domain databases
InterProi | View protein in InterPro IPR005821, Ion_trans_dom IPR003937, K_chnl_volt-dep_KCNQ IPR013821, K_chnl_volt-dep_KCNQ_C IPR005827, K_chnl_volt-dep_KCQN1 IPR028325, VG_K_chnl |
PANTHERi | PTHR11537, PTHR11537, 1 hit PTHR11537:SF144, PTHR11537:SF144, 1 hit |
Pfami | View protein in Pfam PF00520, Ion_trans, 1 hit PF03520, KCNQ_channel, 1 hit |
PRINTSi | PR01460, KCNQ1CHANNEL PR01459, KCNQCHANNEL |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KCNQ1_MOUSE | |
Accessioni | P97414Primary (citable) accession number: P97414 Secondary accession number(s): O88702 Q80VR7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 179 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families