We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - P97393 (RHG05_MOUSE)
Protein
Rho GTPase-activating protein 5
Gene
Arhgap5
Organism
Mus musculus (Mouse)
Status
Functioni
GTPase-activating protein for Rho family members.
By similarityGO - Molecular functioni
- GTPase activator activity Source: UniProtKB-KW
- GTPase activity Source: InterPro
- GTP binding Source: InterPro
- SH2 domain binding Source: MGI
GO - Biological processi
- mammary gland development Source: MGI
- positive regulation of cell migration Source: MGI
- positive regulation of mesenchymal cell proliferation Source: MGI
- regulation of cell size Source: MGI
- signal transduction Source: InterPro
Keywordsi
Molecular function | GTPase activation |
Enzyme and pathway databases
Reactomei | R-MMU-8980692, RHOA GTPase cycle R-MMU-9013026, RHOB GTPase cycle R-MMU-9013106, RHOC GTPase cycle R-MMU-9013148, CDC42 GTPase cycle R-MMU-9013149, RAC1 GTPase cycle R-MMU-9013405, RHOD GTPase cycle R-MMU-9013406, RHOQ GTPase cycle R-MMU-9013408, RHOG GTPase cycle R-MMU-9013409, RHOJ GTPase cycle R-MMU-9013423, RAC3 GTPase cycle R-MMU-9035034, RHOF GTPase cycle R-MMU-9696264, RND3 GTPase cycle R-MMU-9696270, RND2 GTPase cycle R-MMU-9696273, RND1 GTPase cycle |
Names & Taxonomyi
Protein namesi | Recommended name: Rho GTPase-activating protein 5Alternative name(s): Rho-type GTPase-activating protein 5 p190-B |
Gene namesi | Name:Arhgap5 Synonyms:Rhogap5 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1332637, Arhgap5 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Note: Also membrane-associated in a fibrillar pattern that colocalizes with the alpha5-beta1 integrin receptor (ITGA5/ITGB1) for fibronectin.By similarity
Cytosol
- cytosol Source: MGI
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056703 | 1 – 1501 | Rho GTPase-activating protein 5Add BLAST | 1501 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 550 | 3'-nitrotyrosineBy similarity | 1 | |
Modified residuei | 590 | PhosphoserineBy similarity | 1 | |
Modified residuei | 765 | PhosphoserineBy similarity | 1 | |
Modified residuei | 951 | PhosphoserineBy similarity | 1 | |
Modified residuei | 968 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1115 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1194 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1201 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1217 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Nitration, PhosphoproteinProteomic databases
EPDi | P97393 |
jPOSTi | P97393 |
MaxQBi | P97393 |
PaxDbi | P97393 |
PeptideAtlasi | P97393 |
PRIDEi | P97393 |
ProteomicsDBi | 253274 |
PTM databases
iPTMneti | P97393 |
PhosphoSitePlusi | P97393 |
Expressioni
Tissue specificityi
Expressed in spinal cord, cerebellum, kidney, testis and lung.1 Publication
Interactioni
Subunit structurei
May interact with RASA1/p120GAP.
By similarityGO - Molecular functioni
- SH2 domain binding Source: MGI
Protein-protein interaction databases
IntActi | P97393, 3 interactors |
MINTi | P97393 |
STRINGi | 10090.ENSMUSP00000106353 |
Miscellaneous databases
RNActi | P97393, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 267 – 325 | FF 1Add BLAST | 59 | |
Domaini | 366 – 420 | FF 2Add BLAST | 55 | |
Domaini | 427 – 481 | FF 3Add BLAST | 55 | |
Domaini | 482 – 548 | FF 4Add BLAST | 67 | |
Domaini | 590 – 763 | pG1 pseudoGTPasePROSITE-ProRule annotationAdd BLAST | 174 | |
Domaini | 779 – 944 | pG2 pseudoGTPasePROSITE-ProRule annotationAdd BLAST | 166 | |
Domaini | 1261 – 1448 | Rho-GAPPROSITE-ProRule annotationAdd BLAST | 188 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 975 – 1004 | DisorderedSequence analysisAdd BLAST | 30 | |
Regioni | 1022 – 1050 | DisorderedSequence analysisAdd BLAST | 29 | |
Regioni | 1069 – 1091 | DisorderedSequence analysisAdd BLAST | 23 | |
Regioni | 1186 – 1253 | DisorderedSequence analysisAdd BLAST | 68 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1024 – 1038 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 1230 – 1247 | Basic residuesSequence analysisAdd BLAST | 18 |
Domaini
The pG1 pseudoGTPase domain does not bind GTP.By similarity
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG4271, Eukaryota |
InParanoidi | P97393 |
Family and domain databases
Gene3Di | 1.10.10.440, 2 hits 1.10.555.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR002713, FF_domain IPR036517, FF_domain_sf IPR027417, P-loop_NTPase IPR039007, pG1 IPR008936, Rho_GTPase_activation_prot IPR032835, RhoGAP-FF1 IPR000198, RhoGAP_dom IPR045786, RhoGAP_pG1_pG2 IPR039006, RhoGAP_pG2 IPR001806, Small_GTPase |
Pfami | View protein in Pfam PF01846, FF, 1 hit PF00071, Ras, 1 hit PF00620, RhoGAP, 1 hit PF16512, RhoGAP-FF1, 1 hit PF19518, RhoGAP_pG1_pG2, 1 hit |
SMARTi | View protein in SMART SM00441, FF, 4 hits SM00324, RhoGAP, 1 hit |
SUPFAMi | SSF48350, SSF48350, 1 hit SSF52540, SSF52540, 1 hit SSF81698, SSF81698, 1 hit |
PROSITEi | View protein in PROSITE PS51676, FF, 4 hits PS51852, PG1, 1 hit PS51853, PG2, 1 hit PS50238, RHOGAP, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
P97393-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMAKNKEPRP PSYTVSVVGL SGTEKDKGNC GVGKSCLCNR FVRSKADEYY
60 70 80 90 100
PEHTSVLSTI DFGGRVVNND HFLYWGDITQ NGEDGVECKI HVIEQTEFID
110 120 130 140 150
DQTFLPHRST NLQPYIKRAA ASKLQSAEKL MYICTDQLGL EQDFEQKQMP
160 170 180 190 200
EGKLNVDGFL LCIDVSQGCN RKFDDQLKFV NNLFVQLSKS KKPVIIAATK
210 220 230 240 250
CDECVDHYLR EVQAFASNKK NLLVVETSAR FNVNIETCFT ALVQMLDKTR
260 270 280 290 300
GKPKIIPYLD AYKTQRQLVV TATDKFEKLV QTVRDYHATW KTVSNKLKNH
310 320 330 340 350
PDYEEYINLE GTRKARNTFS KHIEQLKQEH IRKRREEYIS TLPRAFNTLL
360 370 380 390 400
PDLEEIEHLN WLEALKLMEK RADFQLCFVV LEKTPWDETD HIDKINDRRI
410 420 430 440 450
PFDLLSTLEA EKVYQNHVQH LISEKRRIEM KEKFKKTLEK IQFISPGQPW
460 470 480 490 500
EEVMCFVMED EAFKYITEAD SKEVYGRHQR EIVEKAKEEF QEMLFEHSEL
510 520 530 540 550
FYDLDLNATP SSDKMSEIHT VLSEEPRYKA LQKLAPDRES LLLKHIGFVY
560 570 580 590 600
HPTKETCLSG QYCTDIKVEN LLATSLLEMD HNRVRLYHDS TNIDKVNLFI
610 620 630 640 650
LGKDGLAQEL ANEIRTQSTD DEYALDGKIY ELDLRPVDAK SPYILSQLWT
660 670 680 690 700
AAFKPHGCFC VFNSIESLSF IGEFIGKIRT EASQIRKDKY MTNLPFTLIL
710 720 730 740 750
ANQRDSISKN LPILRHQGQQ LANKLQCPFV DVPTGTYPRK FNESQIKQAL
760 770 780 790 800
RGVLESVKHN LDVVSPVPIN KDVSEADLRI VMCAMCGDPF SVDLILSPFL
810 820 830 840 850
DSHSCSAAQA GQNNSLMLDK IIGEKRRRIQ ITILSYHSSI GVRKDELVHG
860 870 880 890 900
YILVYSAKRK ASMGMLRAFL SEVQDTIPVQ LVAVTDSQAD FFENEAIKEL
910 920 930 940 950
MTEGEHIATE ITAKFTALYS LSQYHRQTEV FTLFFSDVLE KKNMIENSYL
960 970 980 990 1000
SDNTRESTHQ SEDVFLPSPR DCFPYNNYPD SDDDTEAPPP YSPIGDDVQL
1010 1020 1030 1040 1050
LPTPSDRSRY RLDLEGNEYP VHSTPNCHDH ERNHKVPPPI KPKPVVPKTN
1060 1070 1080 1090 1100
VKKLDPNLLK TIEAGIGKNP RKQTSRVPLA HPEDMDSSDN YVEPLDTIFK
1110 1120 1130 1140 1150
QKGYSDEIYV VPDDSQNRII KIRNSFVNNT QGDEENGFSD PQKVMESVGL
1160 1170 1180 1190 1200
QNININLKLC LVKPSHTTEE HTQMQAMMRL SLLPKKRKGR HRGSEEDPLL
1210 1220 1230 1240 1250
SPVETWKGGI DNPAITSDQE VDDKKIKKKT HKVKEDKKQK KKTKTFNPPT
1260 1270 1280 1290 1300
RRNWESNYFG MPLQDLVTAE KPIPLFVEKC VEFIEDTGLC TEGLYRVSGN
1310 1320 1330 1340 1350
KTDQDNIQKQ FDQDHNINLA SMEVTVNAVA GALKAFFADL PDPLIPYSLH
1360 1370 1380 1390 1400
PELLEAAKIP DKTERFHALK EIVKKFHPVN YDVFRYVITH LNRVSQQNKI
1410 1420 1430 1440 1450
NLMTADNLSI CFWPTLMRPD FENREFLSTT KIHQSVVETF IQQCQFFFYN
1460 1470 1480 1490 1500
GEIVETANTV APPPTSNPGQ LVESMVPLQL PPPLQPQLIQ PQLQTDPLGI
I
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PYT0 | E9PYT0_MOUSE | Rho GTPase-activating protein 5 | Arhgap5 | 1,503 | Annotation score: | ||
A0A1W2P6H9 | A0A1W2P6H9_MOUSE | Rho GTPase-activating protein 5 | Arhgap5 | 241 | Annotation score: |
Sequence cautioni
The sequence AK041016 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 71 – 72 | HF → SL in AAD12768 (PubMed:9838117).Curated | 2 | |
Sequence conflicti | 93 | I → N in AAD12768 (PubMed:9838117).Curated | 1 | |
Sequence conflicti | 118 | R → H in AAD12768 (PubMed:9838117).Curated | 1 | |
Sequence conflicti | 348 – 349 | TL → SV in AAD12768 (PubMed:9838117).Curated | 2 | |
Sequence conflicti | 416 | N → K in AAD12768 (PubMed:9838117).Curated | 1 | |
Sequence conflicti | 629 | I → F in AAD12768 (PubMed:9838117).Curated | 1 | |
Sequence conflicti | 681 | Missing in AAD12768 (PubMed:9838117).Curated | 1 | |
Sequence conflicti | 787 | G → A in AAD12768 (PubMed:9838117).Curated | 1 | |
Sequence conflicti | 793 | D → H in AAD12768 (PubMed:9838117).Curated | 1 | |
Sequence conflicti | 849 | H → T in AAD12768 (PubMed:9838117).Curated | 1 | |
Sequence conflicti | 1079 – 1081 | LAH → FG in AAD12768 (PubMed:9838117).Curated | 3 | |
Sequence conflicti | 1092 | V → A in AAD12768 (PubMed:9838117).Curated | 1 | |
Sequence conflicti | 1140 | D → DR in AAD12768 (PubMed:9838117).Curated | 1 | |
Sequence conflicti | 1153 | I → T in AAD12768 (PubMed:9838117).Curated | 1 | |
Sequence conflicti | 1185 | K → KP in AAD12768 (PubMed:9838117).Curated | 1 | |
Sequence conflicti | 1413 – 1415 | WPT → GQP in AAD12768 (PubMed:9838117).Curated | 3 | |
Sequence conflicti | 1463 | P → Q in AAD12768 (PubMed:9838117).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U67160 Genomic DNA Translation: AAD12768.1 AC114002 Genomic DNA No translation available. AC155819 Genomic DNA No translation available. AK041016 mRNA No translation available. |
PIRi | T42724 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U67160 Genomic DNA Translation: AAD12768.1 AC114002 Genomic DNA No translation available. AC155819 Genomic DNA No translation available. AK041016 mRNA No translation available. |
PIRi | T42724 |
3D structure databases
BMRBi | P97393 |
SMRi | P97393 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P97393, 3 interactors |
MINTi | P97393 |
STRINGi | 10090.ENSMUSP00000106353 |
PTM databases
iPTMneti | P97393 |
PhosphoSitePlusi | P97393 |
Proteomic databases
EPDi | P97393 |
jPOSTi | P97393 |
MaxQBi | P97393 |
PaxDbi | P97393 |
PeptideAtlasi | P97393 |
PRIDEi | P97393 |
ProteomicsDBi | 253274 |
Organism-specific databases
MGIi | MGI:1332637, Arhgap5 |
Phylogenomic databases
eggNOGi | KOG4271, Eukaryota |
InParanoidi | P97393 |
Enzyme and pathway databases
Reactomei | R-MMU-8980692, RHOA GTPase cycle R-MMU-9013026, RHOB GTPase cycle R-MMU-9013106, RHOC GTPase cycle R-MMU-9013148, CDC42 GTPase cycle R-MMU-9013149, RAC1 GTPase cycle R-MMU-9013405, RHOD GTPase cycle R-MMU-9013406, RHOQ GTPase cycle R-MMU-9013408, RHOG GTPase cycle R-MMU-9013409, RHOJ GTPase cycle R-MMU-9013423, RAC3 GTPase cycle R-MMU-9035034, RHOF GTPase cycle R-MMU-9696264, RND3 GTPase cycle R-MMU-9696270, RND2 GTPase cycle R-MMU-9696273, RND1 GTPase cycle |
Miscellaneous databases
ChiTaRSi | Arhgap5, mouse |
PROi | PR:P97393 |
RNActi | P97393, protein |
SOURCEi | Search... |
Family and domain databases
Gene3Di | 1.10.10.440, 2 hits 1.10.555.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR002713, FF_domain IPR036517, FF_domain_sf IPR027417, P-loop_NTPase IPR039007, pG1 IPR008936, Rho_GTPase_activation_prot IPR032835, RhoGAP-FF1 IPR000198, RhoGAP_dom IPR045786, RhoGAP_pG1_pG2 IPR039006, RhoGAP_pG2 IPR001806, Small_GTPase |
Pfami | View protein in Pfam PF01846, FF, 1 hit PF00071, Ras, 1 hit PF00620, RhoGAP, 1 hit PF16512, RhoGAP-FF1, 1 hit PF19518, RhoGAP_pG1_pG2, 1 hit |
SMARTi | View protein in SMART SM00441, FF, 4 hits SM00324, RhoGAP, 1 hit |
SUPFAMi | SSF48350, SSF48350, 1 hit SSF52540, SSF52540, 1 hit SSF81698, SSF81698, 1 hit |
PROSITEi | View protein in PROSITE PS51676, FF, 4 hits PS51852, PG1, 1 hit PS51853, PG2, 1 hit PS50238, RHOGAP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RHG05_MOUSE | |
Accessioni | P97393Primary (citable) accession number: P97393 Secondary accession number(s): E9Q0L8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 28, 2003 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 23, 2022 | |
This is version 153 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot