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Protein

Homeobox protein Meis2

Gene

Meis2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in transcriptional regulation. Binds to HOX or PBX proteins to form dimers, or to a DNA-bound dimer of PBX and HOX proteins and thought to have a role in stabilization of the homeoprotein-DNA complex. Isoform Meis2B is required for the activity of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element; MEIS2 is not involved in complex DNA-binding. Probably in complex with PBX1, is involved in transcriptional regulation by KLF4. Isoforms Meis2B and Meis2D can bind to a EPHA8 promoter sequence containing the DNA motif 5'-CGGTCA-3'; in cooperation with a PBX protein (such as PBX2) is proposed to be involved in the transcriptional activation of EPHA8 in the developing midbrain. May be involved in regulation of myeloid differentiation. Can bind to the DNA sequence 5'-TGACAG-3'in the activator ACT sequence of the D(1A) dopamine receptor (DRD1) promoter and activate DRD1 transcription.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi276 – 338Homeobox; TALE-typePROSITE-ProRule annotationAdd BLAST63

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein Meis2
Alternative name(s):
Meis1-related protein 1
Gene namesi
Name:Meis2
Synonyms:Mrg1, Stra10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:108564 Meis2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000491091 – 477Homeobox protein Meis2Add BLAST477

Proteomic databases

MaxQBiP97367
PaxDbiP97367
PRIDEiP97367

PTM databases

iPTMnetiP97367
PhosphoSitePlusiP97367

Expressioni

Tissue specificityi

Displays spatially restricted expression patterns in the developing nervous system, limbs, face, and in various viscera. In adult, it is mainly expressed in the brain and female genital tract, with a different distribution of the alternative splice forms in these organs. Lower expression in lung and only basal level in heart, liver, kidney, spleen, and testis. Expressed in pancreatic islets (beta-cells only) (PubMed:21059917).1 Publication

Developmental stagei

Expressed at high levels in all stages of embryonic development analyzed (7 days to 17 days). First detected around E10.5 in the developing ventrolateral telencephalon. Is found at moderate levels throughout the ventricle zone (VZ) of the entire telencephalon with the exception of the ventro- and dorso-medial regions. The highest expression is detected in the subventricular zone (SVZ) of the lateral ganglionic eminence (LGE) and developing striatum. By E16.5, also found in the cortical plate. Also expressed at high levels in the caudal ganglionic eminence (CGE) and amygdala. Expression in the telencephalon remains unchanged at birth and into adulthood.1 Publication

Inductioni

By retinoic acid.

Gene expression databases

BgeeiENSMUSG00000027210 Expressed in 301 organ(s), highest expression level in dorsal striatum
CleanExiMM_MEIS2
ExpressionAtlasiP97367 baseline and differential
GenevisibleiP97367 MM

Interactioni

Subunit structurei

Monomer and homodimer. Heterodimer with HOXB13 (By similarity). Isoform Meis2A interacts with TLX1. Isoform Meis2B interacts with HOXA13 and PBX1 isoform PBX1b. Isoform Meis2D interacts with SP1, SP3 and KLF4. Isoform Meis2D interacts with PBX1 isoform PBX1a; the interaction partially relieves MEIS2 autoinhibition. Isoform Meis2B is part of a PDX1:PBX1b:MEIS2b complex; Meis2B is recruited by PBX1b and can be replaced by isoform Meis2D in a small fraction of complexes. Can form trimeric complexes including HOXB8 and PBX2 or PBX3.By similarity4 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201487, 8 interactors
CORUMiP97367
IntActiP97367, 1 interactor
STRINGi10090.ENSMUSP00000028639

Structurei

3D structure databases

ProteinModelPortaliP97367
SMRiP97367
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni71 – 191Required for interaction with PBX11 PublicationAdd BLAST121
Regioni299 – 333Interaction with DNABy similarityAdd BLAST35
Regioni340 – 477Transcriptional activation domainBy similarityAdd BLAST138

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi195 – 244Ser/Thr-richAdd BLAST50
Compositional biasi246 – 273Asp/Glu-rich (acidic)Add BLAST28
Compositional biasi266 – 273Poly-Asp8

Sequence similaritiesi

Belongs to the TALE/MEIS homeobox family.Curated

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0773 Eukaryota
ENOG410XPMQ LUCA
GeneTreeiENSGT00550000074260
HOGENOMiHOG000253923
HOVERGENiHBG055193
InParanoidiP97367
KOiK16670
OMAiPGEYVPQ
OrthoDBiEOG091G0KDP
PhylomeDBiP97367
TreeFamiTF318093

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR001356 Homeobox_dom
IPR008422 Homeobox_KN_domain
IPR032453 PKNOX/Meis_N
PfamiView protein in Pfam
PF05920 Homeobox_KN, 1 hit
PF16493 Meis_PKNOX_N, 1 hit
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
PROSITEiView protein in PROSITE
PS50071 HOMEOBOX_2, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket
Note: Additional isoforms seem to exist.

This entry has 4 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform Meis2C (identifier: P97367-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAQRYDELPH YGGMDGVGVP ASMYGDPHAP RPIPPVHHLN HGPPLHATQH
60 70 80 90 100
YGAHAPHPNV MPASMGSAVN DALKRDKDAI YGHPLFPLLA LVFEKCELAT
110 120 130 140 150
CTPREPGVAG GDVCSSDSFN EDIAVFAKQV RAEKPLFSSN PELDNLMIQA
160 170 180 190 200
IQVLRFHLLE LEKVHELCDN FCHRYISCLK GKMPIDLVID ERDGSSKSDH
210 220 230 240 250
EELSGSSTNL ADHNPSSWRD HDDATSTHSA GTPGPSSGGH ASQSGDNSSE
260 270 280 290 300
QGDGLDNSVA SPGTGDDDDP DKDKKRQKKR GIFPKVATNI MRAWLFQHLT
310 320 330 340 350
HPYPSEEQKK QLAQDTGLTI LQVNNWFINA RRRIVQPMID QSNRAGFLLD
360 370 380 390 400
PSVSQGAAYS PEGQPMGSFV LDGQQHMGIR PAGLQSMPGD YVSQGGPMGM
410 420 430 440 450
GMAQPSYTPP QMTPHPTQLR HGPPMHSYLP SHPHHPAMVM HGGPPTHPGM
460 470
TMSAQSPTML NSVDPNVGGQ VMDIHAQ
Length:477
Mass (Da):51,728
Last modified:December 15, 1998 - v2
Checksum:i923610800B647A21
GO
Isoform Meis2A (identifier: P97367-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     384-401: LQSMPGDYVSQGGPMGMG → PMSGMGMNMGMDGQWHYM
     402-477: Missing.

Show »
Length:401
Mass (Da):43,791
Checksum:i0682C0E92326516D
GO
Isoform Meis2B (identifier: P97367-3) [UniParc]FASTAAdd to basket
Also known as: Mrg1A

The sequence of this isoform differs from the canonical sequence as follows:
     346-352: Missing.
     384-401: LQSMPGDYVSQGGPMGMG → PMSGMGMNMGMDGQWHYM
     402-477: Missing.

Show »
Length:394
Mass (Da):43,061
Checksum:i6BCC0382F0C55296
GO
Isoform Meis2D (identifier: P97367-4) [UniParc]FASTAAdd to basket
Also known as: Mrg1B

The sequence of this isoform differs from the canonical sequence as follows:
     346-352: Missing.

Show »
Length:470
Mass (Da):50,998
Checksum:iC78142B9E15CDDBC
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3TYM2Q3TYM2_MOUSE
Homeobox protein Meis2
Meis2 Mrg1
393Annotation score:
B1AWK4B1AWK4_MOUSE
Homeobox protein Meis2
Meis2
400Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2A → E in AAC52948 (PubMed:8950991).Curated1
Sequence conflicti316T → A in AAC52948 (PubMed:8950991).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002247346 – 352Missing in isoform Meis2B and isoform Meis2D. 2 Publications7
Alternative sequenceiVSP_002248384 – 401LQSMP…PMGMG → PMSGMGMNMGMDGQWHYM in isoform Meis2A and isoform Meis2B. 3 PublicationsAdd BLAST18
Alternative sequenceiVSP_002249402 – 477Missing in isoform Meis2A and isoform Meis2B. 3 PublicationsAdd BLAST76

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57343 mRNA Translation: AAC52948.1
AJ000504 mRNA Translation: CAA04138.1
AJ000505 mRNA Translation: CAA04139.1
AJ000506 mRNA Translation: CAA04140.1
AJ000507 mRNA Translation: CAA04141.1
U68383 mRNA Translation: AAB19193.1
U68384 mRNA Translation: AAB19194.1
CCDSiCCDS16568.1 [P97367-4]
CCDS50666.1 [P97367-3]
CCDS50667.1 [P97367-1]
CCDS50668.1 [P97367-2]
RefSeqiNP_001129544.1, NM_001136072.2 [P97367-3]
NP_001153039.1, NM_001159567.1 [P97367-2]
NP_001153040.1, NM_001159568.1 [P97367-1]
NP_034955.1, NM_010825.3 [P97367-4]
UniGeneiMm.247566
Mm.261124
Mm.471935

Genome annotation databases

EnsembliENSMUST00000028639; ENSMUSP00000028639; ENSMUSG00000027210 [P97367-1]
ENSMUST00000102538; ENSMUSP00000099597; ENSMUSG00000027210 [P97367-4]
ENSMUST00000110907; ENSMUSP00000106532; ENSMUSG00000027210 [P97367-2]
ENSMUST00000110908; ENSMUSP00000106533; ENSMUSG00000027210 [P97367-3]
GeneIDi17536
KEGGimmu:17536
UCSCiuc008lqw.2 mouse [P97367-2]
uc008lqy.2 mouse [P97367-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57343 mRNA Translation: AAC52948.1
AJ000504 mRNA Translation: CAA04138.1
AJ000505 mRNA Translation: CAA04139.1
AJ000506 mRNA Translation: CAA04140.1
AJ000507 mRNA Translation: CAA04141.1
U68383 mRNA Translation: AAB19193.1
U68384 mRNA Translation: AAB19194.1
CCDSiCCDS16568.1 [P97367-4]
CCDS50666.1 [P97367-3]
CCDS50667.1 [P97367-1]
CCDS50668.1 [P97367-2]
RefSeqiNP_001129544.1, NM_001136072.2 [P97367-3]
NP_001153039.1, NM_001159567.1 [P97367-2]
NP_001153040.1, NM_001159568.1 [P97367-1]
NP_034955.1, NM_010825.3 [P97367-4]
UniGeneiMm.247566
Mm.261124
Mm.471935

3D structure databases

ProteinModelPortaliP97367
SMRiP97367
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201487, 8 interactors
CORUMiP97367
IntActiP97367, 1 interactor
STRINGi10090.ENSMUSP00000028639

PTM databases

iPTMnetiP97367
PhosphoSitePlusiP97367

Proteomic databases

MaxQBiP97367
PaxDbiP97367
PRIDEiP97367

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028639; ENSMUSP00000028639; ENSMUSG00000027210 [P97367-1]
ENSMUST00000102538; ENSMUSP00000099597; ENSMUSG00000027210 [P97367-4]
ENSMUST00000110907; ENSMUSP00000106532; ENSMUSG00000027210 [P97367-2]
ENSMUST00000110908; ENSMUSP00000106533; ENSMUSG00000027210 [P97367-3]
GeneIDi17536
KEGGimmu:17536
UCSCiuc008lqw.2 mouse [P97367-2]
uc008lqy.2 mouse [P97367-1]

Organism-specific databases

CTDi4212
MGIiMGI:108564 Meis2

Phylogenomic databases

eggNOGiKOG0773 Eukaryota
ENOG410XPMQ LUCA
GeneTreeiENSGT00550000074260
HOGENOMiHOG000253923
HOVERGENiHBG055193
InParanoidiP97367
KOiK16670
OMAiPGEYVPQ
OrthoDBiEOG091G0KDP
PhylomeDBiP97367
TreeFamiTF318093

Miscellaneous databases

ChiTaRSiMeis2 mouse
PROiPR:P97367
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027210 Expressed in 301 organ(s), highest expression level in dorsal striatum
CleanExiMM_MEIS2
ExpressionAtlasiP97367 baseline and differential
GenevisibleiP97367 MM

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR001356 Homeobox_dom
IPR008422 Homeobox_KN_domain
IPR032453 PKNOX/Meis_N
PfamiView protein in Pfam
PF05920 Homeobox_KN, 1 hit
PF16493 Meis_PKNOX_N, 1 hit
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
PROSITEiView protein in PROSITE
PS50071 HOMEOBOX_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMEIS2_MOUSE
AccessioniPrimary (citable) accession number: P97367
Secondary accession number(s): O35676
, O35677, P97403, P97404
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 15, 1998
Last modified: September 12, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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