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Protein

Kinesin-like protein KIF20A

Gene

Kif20a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle (By similarity). Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi159 – 166ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity Source: GO_Central
  • ATP binding Source: UniProtKB-KW
  • microtubule binding Source: GO_Central
  • microtubule motor activity Source: GO_Central
  • protein kinase binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionMotor protein
Biological processProtein transport, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2132295 MHC class II antigen presentation
R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-MMU-68884 Mitotic Telophase/Cytokinesis
R-MMU-983189 Kinesins

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-like protein KIF20A
Alternative name(s):
Kinesin-like protein 174
Rab6-interacting kinesin-like protein
Rabkinesin-6
Gene namesi
Name:Kif20a
Synonyms:Rab6kifl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1201682 Kif20a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Golgi apparatus, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001254612 – 887Kinesin-like protein KIF20AAdd BLAST886

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei7PhosphoserineBy similarity1
Modified residuei14PhosphoserineBy similarity1
Modified residuei21PhosphoserineBy similarity1
Modified residuei527Phosphoserine; by PLK1By similarity1
Modified residuei683PhosphoserineBy similarity1
Modified residuei823PhosphoserineBy similarity1
Modified residuei855PhosphothreonineBy similarity1
Modified residuei865PhosphoserineBy similarity1
Modified residuei876PhosphoserineBy similarity1
Modified residuei881PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by PLK1 at Ser-527 during mitosis, creating a docking site for PLK1 and recruiting PLK1 at central spindle.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP97329
MaxQBiP97329
PaxDbiP97329
PeptideAtlasiP97329
PRIDEiP97329

PTM databases

iPTMnetiP97329
PhosphoSitePlusiP97329

Expressioni

Tissue specificityi

Ubiquitously expressed, with highest levels in spleen and testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000003779 Expressed in 202 organ(s), highest expression level in lung
CleanExiMM_KIF20A
GenevisibleiP97329 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202552, 11 interactors
IntActiP97329, 10 interactors
STRINGi10090.ENSMUSP00000130045

Structurei

Secondary structure

1887
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP97329
SMRiP97329
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 506Kinesin motorPROSITE-ProRule annotationAdd BLAST444

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni805 – 887GlobularSequence analysisAdd BLAST83

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili559 – 804Sequence analysisAdd BLAST246

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0247 Eukaryota
COG5059 LUCA
GeneTreeiENSGT00930000150825
HOGENOMiHOG000054207
HOVERGENiHBG052246
InParanoidiP97329
KOiK10402
OMAiDIRFSIW
OrthoDBiEOG091G00AY
PhylomeDBiP97329
TreeFamiTF105232

Family and domain databases

Gene3Di3.40.850.10, 2 hits
InterProiView protein in InterPro
IPR027326 KIF20A
IPR027640 Kinesin-like_fam
IPR019821 Kinesin_motor_CS
IPR001752 Kinesin_motor_dom
IPR036961 Kinesin_motor_dom_sf
IPR027417 P-loop_NTPase
PANTHERiPTHR24115 PTHR24115, 1 hit
PTHR24115:SF352 PTHR24115:SF352, 1 hit
PfamiView protein in Pfam
PF00225 Kinesin, 1 hit
PRINTSiPR00380 KINESINHEAVY
SMARTiView protein in SMART
SM00129 KISc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00411 KINESIN_MOTOR_1, 1 hit
PS50067 KINESIN_MOTOR_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97329-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSHRILSPPA GLLSDEDVVD SPILESTAAD LRSVVRKDLL SDCSVISASL
60 70 80 90 100
EDKQALLEDT SEKVKVYLRI RPFLTSELDR QEDQGCVCIE NTETLVLQAP
110 120 130 140 150
KDSFALKSNE RGVGQATHKF TFSQIFGPEV GQVAFFNLTM KEMVKDVLKG
160 170 180 190 200
QNWLIYTYGV TNSGKTYTIQ GTSKDAGILP QSLALIFNSL QGQLHPTPDL
210 220 230 240 250
KPLLSNEVIW LDSKQIRQEE MKKLSLLIGG LQEEELSTSV KKRVHTESRI
260 270 280 290 300
GASNSFDSGV AGLSSTSQFT SSSQLDETSQ LWAQPDTVPV SVPADIRFSV
310 320 330 340 350
WISFFEIYNE LLYDLLEPPS HQHKRQTLRL CEDQNGNPYV KDLNWIHVRD
360 370 380 390 400
VEEAWKLLKV GRKNQSFAST HMNQQSSRSH SIFSIRILHL QGEGDIVPKI
410 420 430 440 450
SELSLCDLAG SERCKHQKSG ERLKEAGNIN TSLHTLGRCI AALRQNQQNR
460 470 480 490 500
SKQNLIPFRD SKLTRVFQGF FTGRGRSCMI VNVNPCASTY DETLHAAKFS
510 520 530 540 550
ALASQLVHAP PVHLGIPSLH SFIKKHSPQV GPGLEKEDKA DSDLEDSPED
560 570 580 590 600
EADVSVYGKE ELLQVVEAMK ALLLKERQEK LQLEIQLREE ICNEMVEQMQ
610 620 630 640 650
QREQWCSERL DNQKELMEEL YEEKLKILKE SLTTFYQEQI QERDEKIEEL
660 670 680 690 700
ETLLQEAKQQ PAAQQSGGLS LLRRSQRLAA SASTQQFQEV KAELEQCKTE
710 720 730 740 750
LSSTTAELHK YQQVLKPPPP AKPFTIDVDK KLEEGQKNIR LLRTELQKLG
760 770 780 790 800
QSLQSAERAC CHSTGAGKLR QALTNCDDIL IKQNQTLAEL QNNMVLVKLD
810 820 830 840 850
LQKKAACIAE QYHTVLKLQG QASAKKRLGA NQENQQPNHQ PPGKKPFLRN
860 870 880
LLPRTPTCQS STDSSPYARI LRSRHSPLLK SPFGKKY
Length:887
Mass (Da):99,876
Last modified:May 1, 1997 - v1
Checksum:i7143CCB261A4EA15
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09632 mRNA Translation: CAA70845.1
AK083412 mRNA Translation: BAC38906.1
AK084732 mRNA Translation: BAC39267.1
AK144844 mRNA Translation: BAE26095.1
BC060608 mRNA Translation: AAH60608.1
CCDSiCCDS29131.1
RefSeqiNP_001159878.1, NM_001166406.1
NP_001159879.1, NM_001166407.1
NP_033030.1, NM_009004.4
UniGeneiMm.258846

Genome annotation databases

EnsembliENSMUST00000166044; ENSMUSP00000132659; ENSMUSG00000003779
ENSMUST00000167161; ENSMUSP00000130045; ENSMUSG00000003779
GeneIDi19348
KEGGimmu:19348
UCSCiuc008ela.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09632 mRNA Translation: CAA70845.1
AK083412 mRNA Translation: BAC38906.1
AK084732 mRNA Translation: BAC39267.1
AK144844 mRNA Translation: BAE26095.1
BC060608 mRNA Translation: AAH60608.1
CCDSiCCDS29131.1
RefSeqiNP_001159878.1, NM_001166406.1
NP_001159879.1, NM_001166407.1
NP_033030.1, NM_009004.4
UniGeneiMm.258846

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5LEFX-ray2.09C/D603-665[»]
5ND2electron microscopy5.80C21-521[»]
5ND3electron microscopy6.10C21-521[»]
5ND4electron microscopy4.40C21-521[»]
5ND7electron microscopy7.90C21-521[»]
ProteinModelPortaliP97329
SMRiP97329
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202552, 11 interactors
IntActiP97329, 10 interactors
STRINGi10090.ENSMUSP00000130045

PTM databases

iPTMnetiP97329
PhosphoSitePlusiP97329

Proteomic databases

EPDiP97329
MaxQBiP97329
PaxDbiP97329
PeptideAtlasiP97329
PRIDEiP97329

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000166044; ENSMUSP00000132659; ENSMUSG00000003779
ENSMUST00000167161; ENSMUSP00000130045; ENSMUSG00000003779
GeneIDi19348
KEGGimmu:19348
UCSCiuc008ela.2 mouse

Organism-specific databases

CTDi10112
MGIiMGI:1201682 Kif20a

Phylogenomic databases

eggNOGiKOG0247 Eukaryota
COG5059 LUCA
GeneTreeiENSGT00930000150825
HOGENOMiHOG000054207
HOVERGENiHBG052246
InParanoidiP97329
KOiK10402
OMAiDIRFSIW
OrthoDBiEOG091G00AY
PhylomeDBiP97329
TreeFamiTF105232

Enzyme and pathway databases

ReactomeiR-MMU-2132295 MHC class II antigen presentation
R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-MMU-68884 Mitotic Telophase/Cytokinesis
R-MMU-983189 Kinesins

Miscellaneous databases

ChiTaRSiKif20a mouse
PROiPR:P97329
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000003779 Expressed in 202 organ(s), highest expression level in lung
CleanExiMM_KIF20A
GenevisibleiP97329 MM

Family and domain databases

Gene3Di3.40.850.10, 2 hits
InterProiView protein in InterPro
IPR027326 KIF20A
IPR027640 Kinesin-like_fam
IPR019821 Kinesin_motor_CS
IPR001752 Kinesin_motor_dom
IPR036961 Kinesin_motor_dom_sf
IPR027417 P-loop_NTPase
PANTHERiPTHR24115 PTHR24115, 1 hit
PTHR24115:SF352 PTHR24115:SF352, 1 hit
PfamiView protein in Pfam
PF00225 Kinesin, 1 hit
PRINTSiPR00380 KINESINHEAVY
SMARTiView protein in SMART
SM00129 KISc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00411 KINESIN_MOTOR_1, 1 hit
PS50067 KINESIN_MOTOR_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKI20A_MOUSE
AccessioniPrimary (citable) accession number: P97329
Secondary accession number(s): Q542M4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1997
Last modified: November 7, 2018
This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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