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UniProtKB - P97325 (SIAT6_MOUSE)
Protein
CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase
Gene
St3gal3
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the formation of the NeuAc-alpha-2,3-Gal-beta-1,4-GlcNAc-, NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc- and NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- sequences found in terminal carbohydrate groups of glycoproteins and glycolipids. The highest activity is toward Gal-beta-1,3-GlcNAc and the lowest toward Gal-beta-1,3-GalNAc.
1 PublicationCatalytic activityi
- a β-D-galactosyl-(1→4)-N-acetyl-β-D-glucosaminyl derivative + CMP-N-acetyl-β-neuraminate = an N-acetyl-α-neuraminyl-(2→3)-β-D-galactosyl-(1→4)-N-acetyl-β-D-glucosaminyl derivative + CMP + H+1 PublicationEC:2.4.99.61 Publication
Kineticsi
Relative Vmax is 4:2:1 for Gal-beta-1,3-GlcNAc, Gal-beta-1,4-GlcNAc and Gal-beta-1,3-GalNAc as substrate, respectively.1 Publication
- KM=0.32 mM for Gal-beta-1,3-GlcNAc1 Publication
- KM=3.0 mM for Gal-beta-1,4-GlcNAc1 Publication
- KM=2.2 mM for Gal-beta-1,3-GalNAc1 Publication
pH dependencei
Optimum pH is 6.4.1 Publication
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
GO - Molecular functioni
- beta-galactoside (CMP) alpha-2,3-sialyltransferase activity Source: MGI
- N-acetyllactosaminide alpha-2,3-sialyltransferase activity Source: UniProtKB-EC
- sialyltransferase activity Source: GO_Central
GO - Biological processi
- ganglioside biosynthetic process via lactosylceramide Source: UniProtKB
- protein glycosylation Source: MGI
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
BRENDAi | 2.4.99.2, 3474 2.4.99.6, 3474 2.4.99.8, 3474 |
Reactomei | R-MMU-2022854, Keratan sulfate biosynthesis R-MMU-4085001, Sialic acid metabolism R-MMU-9037629, Lewis blood group biosynthesis R-MMU-977068, Termination of O-glycan biosynthesis |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GT29, Glycosyltransferase Family 29 |
Names & Taxonomyi
Protein namesi | Recommended name: CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase (EC:2.4.99.61 Publication)Alternative name(s): Beta-galactoside alpha-2,3-sialyltransferase 3 Short name: Alpha 2,3-ST 3 Gal beta-1,3(4) GlcNAc alpha-2,3 sialyltransferase N-acetyllactosaminide alpha-2,3-sialyltransferase ST3Gal III Short name: ST3GalIII ST3N Sialyltransferase 6 |
Gene namesi | Name:St3gal3 Synonyms:Siat3, Siat6 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1316659, St3gal3 |
VEuPathDBi | HostDB:ENSMUSG00000028538 |
Subcellular locationi
Golgi apparatus
Other locations
Golgi apparatus
- Golgi cisterna membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 8 | CytoplasmicSequence analysis | 8 | |
Transmembranei | 9 – 28 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 20 | |
Topological domaini | 29 – 374 | LumenalSequence analysisAdd BLAST | 346 |
Keywords - Cellular componenti
Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000149267 | 1 – 374 | CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferaseAdd BLAST | 374 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 79 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 159 ↔ 313 | By similarity | ||
Glycosylationi | 170 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MaxQBi | P97325 |
PaxDbi | P97325 |
PRIDEi | P97325 |
ProteomicsDBi | 257245 |
PTM databases
GlyGeni | P97325, 2 sites |
iPTMneti | P97325 |
PhosphoSitePlusi | P97325 |
Expressioni
Tissue specificityi
Found in all tissues tested. High expression found in brain, liver, kidney, colon, heart and spleen.1 Publication
Developmental stagei
Developmental regulation only occurs in liver, heart, kidney and spleen.
Gene expression databases
Bgeei | ENSMUSG00000028538, Expressed in large intestine and 295 other tissues |
ExpressionAtlasi | P97325, baseline and differential |
Genevisiblei | P97325, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000030263 |
Miscellaneous databases
RNActi | P97325, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyltransferase 29 family.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2692, Eukaryota |
GeneTreei | ENSGT00940000157285 |
InParanoidi | P97325 |
OMAi | NDINVGR |
OrthoDBi | 891104at2759 |
PhylomeDBi | P97325 |
TreeFami | TF354325 |
Family and domain databases
Gene3Di | 3.90.1480.20, 1 hit |
InterProi | View protein in InterPro IPR001675, Glyco_trans_29 IPR038578, GT29-like_sf IPR012163, Sialyl_trans |
Pfami | View protein in Pfam PF00777, Glyco_transf_29, 1 hit |
PIRSFi | PIRSF005557, Sialyl_trans, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
P97325-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGLLVFVRNL LLALCLFLVL GFLYYSAWKL HLLQWEDSNS LLLSLDSAGQ
60 70 80 90 100
TLGTEYDRLG FLLKLDSKLP AELATKYANF SEGACKPGYA SAMMTAIFPR
110 120 130 140 150
FSKPAPMFLD DSFRKWARIR EFVPPFGIKG QDNLIKAILS VTKEYRLTPA
160 170 180 190 200
LDSLHCRRCI IVGNGGVLAN KSLGSRIDDY DIVIRLNSAP VKGFERDVGS
210 220 230 240 250
KTTLRITYPE GAMQRPEQYE RDSLFVLAGF KWQDFKWLKY IVYKERVSAS
260 270 280 290 300
DGFWKSVATR VPKEPPEIRI LNPYFIQEAA FTLIGLPFNN GLMGRGNIPT
310 320 330 340 350
LGSVAVTMAL HGCDEVAVAG FGYDMNTPNA PLHYYETVRM AAIKESWTHN
360 370
IQREKEFLRK LVKARVITDL SSGI
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ9CZ48 | Q9CZ48_MOUSE | CMP-N-acetylneuraminate-beta-1,4-ga... | St3gal3 Siat6 | 358 | Annotation score: | ||
B1AS81 | B1AS81_MOUSE | CMP-N-acetylneuraminate-beta-1,4-ga... | St3gal3 | 159 | Annotation score: | ||
F6XZQ5 | F6XZQ5_MOUSE | CMP-N-acetylneuraminate-beta-1,4-ga... | St3gal3 | 270 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 78 | A → P in CAA59013 (PubMed:9184827).Curated | 1 | |
Sequence conflicti | 350 | N → S in CAA59013 (PubMed:9184827).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X84234 mRNA Translation: CAA59013.1 AL611952 Genomic DNA No translation available. AL626764 Genomic DNA No translation available. AL627128 Genomic DNA No translation available. CH466552 Genomic DNA Translation: EDL30520.1 CH466552 Genomic DNA Translation: EDL30522.1 BC006710 mRNA Translation: AAH06710.1 |
CCDSi | CCDS18544.1 |
RefSeqi | NP_001155246.1, NM_001161774.2 NP_001272449.1, NM_001285520.1 NP_033202.3, NM_009176.5 |
Genome annotation databases
Ensembli | ENSMUST00000030263; ENSMUSP00000030263; ENSMUSG00000028538 ENSMUST00000106410; ENSMUSP00000102018; ENSMUSG00000028538 |
GeneIDi | 20441 |
KEGGi | mmu:20441 |
UCSCi | uc008ujk.3, mouse |
Similar proteinsi
Cross-referencesi
Web resourcesi
Functional Glycomics Gateway - GTase ST3Gal III |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X84234 mRNA Translation: CAA59013.1 AL611952 Genomic DNA No translation available. AL626764 Genomic DNA No translation available. AL627128 Genomic DNA No translation available. CH466552 Genomic DNA Translation: EDL30520.1 CH466552 Genomic DNA Translation: EDL30522.1 BC006710 mRNA Translation: AAH06710.1 |
CCDSi | CCDS18544.1 |
RefSeqi | NP_001155246.1, NM_001161774.2 NP_001272449.1, NM_001285520.1 NP_033202.3, NM_009176.5 |
3D structure databases
SMRi | P97325 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000030263 |
Protein family/group databases
CAZyi | GT29, Glycosyltransferase Family 29 |
PTM databases
GlyGeni | P97325, 2 sites |
iPTMneti | P97325 |
PhosphoSitePlusi | P97325 |
Proteomic databases
MaxQBi | P97325 |
PaxDbi | P97325 |
PRIDEi | P97325 |
ProteomicsDBi | 257245 |
Protocols and materials databases
Antibodypediai | 32385, 166 antibodies from 26 providers |
DNASUi | 20441 |
Genome annotation databases
Ensembli | ENSMUST00000030263; ENSMUSP00000030263; ENSMUSG00000028538 ENSMUST00000106410; ENSMUSP00000102018; ENSMUSG00000028538 |
GeneIDi | 20441 |
KEGGi | mmu:20441 |
UCSCi | uc008ujk.3, mouse |
Organism-specific databases
CTDi | 6487 |
MGIi | MGI:1316659, St3gal3 |
VEuPathDBi | HostDB:ENSMUSG00000028538 |
Phylogenomic databases
eggNOGi | KOG2692, Eukaryota |
GeneTreei | ENSGT00940000157285 |
InParanoidi | P97325 |
OMAi | NDINVGR |
OrthoDBi | 891104at2759 |
PhylomeDBi | P97325 |
TreeFami | TF354325 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
BRENDAi | 2.4.99.2, 3474 2.4.99.6, 3474 2.4.99.8, 3474 |
Reactomei | R-MMU-2022854, Keratan sulfate biosynthesis R-MMU-4085001, Sialic acid metabolism R-MMU-9037629, Lewis blood group biosynthesis R-MMU-977068, Termination of O-glycan biosynthesis |
Miscellaneous databases
BioGRID-ORCSi | 20441, 3 hits in 65 CRISPR screens |
ChiTaRSi | St3gal3, mouse |
PROi | PR:P97325 |
RNActi | P97325, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000028538, Expressed in large intestine and 295 other tissues |
ExpressionAtlasi | P97325, baseline and differential |
Genevisiblei | P97325, MM |
Family and domain databases
Gene3Di | 3.90.1480.20, 1 hit |
InterProi | View protein in InterPro IPR001675, Glyco_trans_29 IPR038578, GT29-like_sf IPR012163, Sialyl_trans |
Pfami | View protein in Pfam PF00777, Glyco_transf_29, 1 hit |
PIRSFi | PIRSF005557, Sialyl_trans, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SIAT6_MOUSE | |
Accessioni | P97325Primary (citable) accession number: P97325 Secondary accession number(s): Q922X5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 23, 2022 | |
This is version 155 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families