UniProtKB - P97311 (MCM6_MOUSE)
Protein
DNA replication licensing factor MCM6
Gene
Mcm6
Organism
Mus musculus (Mouse)
Status
Functioni
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.By similarity
Catalytic activityi
- EC:3.6.4.12
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 396 – 403 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- DNA helicase activity Source: UniProtKB-EC
- DNA replication origin binding Source: GO_Central
- identical protein binding Source: MGI
- single-stranded DNA binding Source: MGI
GO - Biological processi
- DNA replication initiation Source: InterPro
- DNA unwinding involved in DNA replication Source: MGI
- double-strand break repair via break-induced replication Source: GO_Central
- mitotic DNA replication Source: GO_Central
- pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Helicase, Hydrolase |
Biological process | Cell cycle, DNA replication |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-176187, Activation of ATR in response to replication stress R-MMU-68867, Assembly of the pre-replicative complex R-MMU-68949, Orc1 removal from chromatin R-MMU-68962, Activation of the pre-replicative complex R-MMU-69052, Switching of origins to a post-replicative state |
Names & Taxonomyi
Protein namesi | Recommended name: DNA replication licensing factor MCM6 (EC:3.6.4.12)Alternative name(s): Mis5 homolog |
Gene namesi | Name:Mcm6 Synonyms:Mcmd6, Mis5 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1298227, Mcm6 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Other locations
- Chromosome By similarity
Note: Binds to chromatin during G1 and detach from it during S phase.By similarity
Nucleus
- CMG complex Source: UniProtKB
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- MCM complex Source: UniProtKB
Keywords - Cellular componenti
Chromosome, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194114 | 1 – 821 | DNA replication licensing factor MCM6Add BLAST | 821 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 13 | PhosphoserineBy similarity | 1 | |
Modified residuei | 219 | PhosphoserineBy similarity | 1 | |
Modified residuei | 271 | PhosphoserineCombined sources | 1 | |
Modified residuei | 278 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 643 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 689 | PhosphoserineCombined sources | 1 | |
Modified residuei | 704 | PhosphoserineCombined sources | 1 | |
Modified residuei | 762 | PhosphoserineCombined sources | 1 | |
Modified residuei | 791 | PhosphothreonineCombined sources | 1 |
Post-translational modificationi
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.By similarity
Keywords - PTMi
Acetylation, Glycoprotein, PhosphoproteinProteomic databases
EPDi | P97311 |
jPOSTi | P97311 |
PaxDbi | P97311 |
PeptideAtlasi | P97311 |
PRIDEi | P97311 |
ProteomicsDBi | 295840 |
PTM databases
iPTMneti | P97311 |
PhosphoSitePlusi | P97311 |
SwissPalmi | P97311 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000026355, Expressed in metanephric renal vesicle and 273 other tissues |
Genevisiblei | P97311, MM |
Interactioni
Subunit structurei
Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5. May interact with MCM10.
Interacts with TIPIN.
Interacts with CDT1.
Interacts with MCMBP.
Interacts with DDI2 (By similarity).
Component of the CMG helicase complex, composed of the MCM2-7 complex, the GINS complex and CDC45 (By similarity).
By similarityBinary interactionsi
P97311
With | #Exp. | IntAct |
---|---|---|
Cdt1 [Q8R4E9] | 2 | EBI-457132,EBI-457043 |
GO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 201349, 17 interactors |
ComplexPortali | CPX-2941, MCM complex |
CORUMi | P97311 |
IntActi | P97311, 7 interactors |
MINTi | P97311 |
STRINGi | 10090.ENSMUSP00000027601 |
Miscellaneous databases
RNActi | P97311, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 346 – 553 | MCMAdd BLAST | 208 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 528 – 531 | Arginine finger | 4 |
Sequence similaritiesi
Belongs to the MCM family.Curated
Phylogenomic databases
eggNOGi | KOG0480, Eukaryota |
GeneTreei | ENSGT01010000222297 |
InParanoidi | P97311 |
OrthoDBi | 266497at2759 |
PhylomeDBi | P97311 |
TreeFami | TF105646 |
Family and domain databases
InterProi | View protein in InterPro IPR031327, MCM IPR008049, MCM6 IPR041024, Mcm6_C IPR018525, MCM_CS IPR001208, MCM_dom IPR041562, MCM_lid IPR027925, MCM_N IPR033762, MCM_OB IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase |
PANTHERi | PTHR11630, PTHR11630, 1 hit |
Pfami | View protein in Pfam PF00493, MCM, 1 hit PF18263, MCM6_C, 1 hit PF17855, MCM_lid, 1 hit PF14551, MCM_N, 1 hit PF17207, MCM_OB, 1 hit |
PRINTSi | PR01657, MCMFAMILY PR01662, MCMPROTEIN6 |
SMARTi | View protein in SMART SM00350, MCM, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00847, MCM_1, 1 hit PS50051, MCM_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P97311-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDLAAAAEPG AGSQHPEVRD EVAEKCQKLF LDFLEEFQGS DGEIKYLQFA
60 70 80 90 100
EELIRPERNT LVVSFADLEQ FNQQLSTTIQ EEFYRVYPYL CRALKTFVKD
110 120 130 140 150
RKEIPFAKDF YVAFQDLPTR HKIRELTSSR IGLLTRISGQ VVRTHPVHPE
160 170 180 190 200
LVSGTFLCLD CQTVIKDVEQ QFKYTQPNIC RNPVCANRKR FLLDTNKSRF
210 220 230 240 250
VDFQKVRIQE TQAELPRGSI PRSLEVILRA EAVESAQAGD RCDFTGALIV
260 270 280 290 300
VPDVSKLSTP GARAETNSRV SGADGYETEG IRGLRALGVR DLSYRLVFLA
310 320 330 340 350
CHVAPTNPRF GGKELRDEEQ TAESIKNQMT VKEWEKVFEM SQDKNLYHNL
360 370 380 390 400
CTSLFPTIHG NDEVKRGVLL MLFGGVPKTT GEGTSLRGDI NVCIVGDPST
410 420 430 440 450
AKSQFLKHVD EFSPRAVYTS GKASSAAGLT AAVVRDEESH EFVIEAGALM
460 470 480 490 500
LADNGVCCID EFDKMDMRDQ VAIHEAMEQQ TISITKAGVK ATLNARTSIL
510 520 530 540 550
AAANPVSGHY DRSKSLKQNI NLSAPIMSRF DLFFILVDEC NEVTDYAIAR
560 570 580 590 600
RIVDLHSRIE ESIDRVYSLD DIRRYLLFAR QFKPKISKES EDFIVEQYKR
610 620 630 640 650
LRQRDGSGVT KSSWRITVRQ LESMIRLSES MARMHCCDEV QPKHVKEAFR
660 670 680 690 700
LLNKSIIRVE TPDVNLDQEE EIQMETDEGQ GGVNGHADSP APVNRFNGSS
710 720 730 740 750
EDASQETVSK PSLRLGFAEY CRISNLIVLH LRKMEEEEDE SALKRSELVN
760 770 780 790 800
WYLKEIESEI DSEEELINKK TIIEKVVHRL THYDHVLIEL TQAGLKGSSE
810 820
GSESYEEDPY LVVNPNYLLE D
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ3ULG5 | Q3ULG5_MOUSE | DNA helicase | Mcm6 | 794 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D86726 mRNA Translation: BAA13159.1 BC050886 mRNA Translation: AAH50886.2 BC057584 mRNA Translation: AAH57584.1 |
CCDSi | CCDS15252.1 |
RefSeqi | NP_032593.1, NM_008567.2 |
Genome annotation databases
Ensembli | ENSMUST00000027601; ENSMUSP00000027601; ENSMUSG00000026355 |
GeneIDi | 17219 |
KEGGi | mmu:17219 |
UCSCi | uc007cln.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D86726 mRNA Translation: BAA13159.1 BC050886 mRNA Translation: AAH50886.2 BC057584 mRNA Translation: AAH57584.1 |
CCDSi | CCDS15252.1 |
RefSeqi | NP_032593.1, NM_008567.2 |
3D structure databases
SMRi | P97311 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201349, 17 interactors |
ComplexPortali | CPX-2941, MCM complex |
CORUMi | P97311 |
IntActi | P97311, 7 interactors |
MINTi | P97311 |
STRINGi | 10090.ENSMUSP00000027601 |
PTM databases
iPTMneti | P97311 |
PhosphoSitePlusi | P97311 |
SwissPalmi | P97311 |
Proteomic databases
EPDi | P97311 |
jPOSTi | P97311 |
PaxDbi | P97311 |
PeptideAtlasi | P97311 |
PRIDEi | P97311 |
ProteomicsDBi | 295840 |
Protocols and materials databases
Antibodypediai | 1416, 338 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000027601; ENSMUSP00000027601; ENSMUSG00000026355 |
GeneIDi | 17219 |
KEGGi | mmu:17219 |
UCSCi | uc007cln.1, mouse |
Organism-specific databases
CTDi | 4175 |
MGIi | MGI:1298227, Mcm6 |
Phylogenomic databases
eggNOGi | KOG0480, Eukaryota |
GeneTreei | ENSGT01010000222297 |
InParanoidi | P97311 |
OrthoDBi | 266497at2759 |
PhylomeDBi | P97311 |
TreeFami | TF105646 |
Enzyme and pathway databases
Reactomei | R-MMU-176187, Activation of ATR in response to replication stress R-MMU-68867, Assembly of the pre-replicative complex R-MMU-68949, Orc1 removal from chromatin R-MMU-68962, Activation of the pre-replicative complex R-MMU-69052, Switching of origins to a post-replicative state |
Miscellaneous databases
BioGRID-ORCSi | 17219, 22 hits in 50 CRISPR screens |
ChiTaRSi | Mcm6, mouse |
PROi | PR:P97311 |
RNActi | P97311, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026355, Expressed in metanephric renal vesicle and 273 other tissues |
Genevisiblei | P97311, MM |
Family and domain databases
InterProi | View protein in InterPro IPR031327, MCM IPR008049, MCM6 IPR041024, Mcm6_C IPR018525, MCM_CS IPR001208, MCM_dom IPR041562, MCM_lid IPR027925, MCM_N IPR033762, MCM_OB IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase |
PANTHERi | PTHR11630, PTHR11630, 1 hit |
Pfami | View protein in Pfam PF00493, MCM, 1 hit PF18263, MCM6_C, 1 hit PF17855, MCM_lid, 1 hit PF14551, MCM_N, 1 hit PF17207, MCM_OB, 1 hit |
PRINTSi | PR01657, MCMFAMILY PR01662, MCMPROTEIN6 |
SMARTi | View protein in SMART SM00350, MCM, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00847, MCM_1, 1 hit PS50051, MCM_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MCM6_MOUSE | |
Accessioni | P97311Primary (citable) accession number: P97311 Secondary accession number(s): Q80YQ7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | May 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 176 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families