UniProtKB - P97310 (MCM2_MOUSE)
DNA replication licensing factor MCM2
Mcm2
Functioni
Catalytic activityi
- EC:3.6.4.12
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 329 – 355 | C4-typeSequence analysisAdd BLAST | 27 | |
Nucleotide bindingi | 523 – 530 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- DNA helicase activity Source: UniProtKB-EC
- DNA replication origin binding Source: MGI
- enzyme binding Source: BHF-UCL
- histone binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- single-stranded DNA binding Source: GO_Central
GO - Biological processi
- apoptotic process Source: UniProtKB
- cellular response to interleukin-4 Source: MGI
- cochlea development Source: UniProtKB
- DNA replication initiation Source: MGI
- DNA unwinding involved in DNA replication Source: MGI
- double-strand break repair via break-induced replication Source: GO_Central
- mitotic DNA replication initiation Source: GO_Central
- negative regulation of DNA helicase activity Source: InterPro
- nucleosome assembly Source: MGI
- pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Helicase, Hydrolase |
Biological process | Cell cycle, DNA replication |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-176187, Activation of ATR in response to replication stress R-MMU-68867, Assembly of the pre-replicative complex R-MMU-68949, Orc1 removal from chromatin R-MMU-68962, Activation of the pre-replicative complex R-MMU-69052, Switching of origins to a post-replicative state |
Names & Taxonomyi
Protein namesi | Recommended name: DNA replication licensing factor MCM2 (EC:3.6.4.12)Alternative name(s): Minichromosome maintenance protein 2 homolog Nuclear protein BM28 |
Gene namesi | Name:Mcm2 Synonyms:Bm28, Cdcl1, Kiaa0030, Mcmd2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:105380, Mcm2 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Other locations
- Chromosome By similarity
Note: Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses.By similarity
Cytoskeleton
- microtubule cytoskeleton Source: MGI
Nucleus
- CMG complex Source: UniProtKB
- nuclear origin of replication recognition complex Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- chromatin Source: MGI
- cytoplasm Source: UniProtKB
- MCM complex Source: UniProtKB
Keywords - Cellular componenti
Chromosome, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 237 | L → P: Impairs interaction with KAT7. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000194088 | 2 – 904 | DNA replication licensing factor MCM2Add BLAST | 903 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 12 | PhosphoserineCombined sources | 1 | |
Modified residuei | 21 | PhosphoserineCombined sources | 1 | |
Modified residuei | 25 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 26 | PhosphoserineBy similarity | 1 | |
Modified residuei | 27 | PhosphoserineCombined sources | 1 | |
Modified residuei | 32 | PhosphoserineBy similarity | 1 | |
Modified residuei | 39 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 40 | Phosphoserine; by CDC7By similarity | 1 | |
Modified residuei | 41 | PhosphoserineCombined sources | 1 | |
Modified residuei | 53 | Phosphoserine; by CDC7By similarity | 1 | |
Modified residuei | 59 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 108 | PhosphoserineCombined sources | 1 | |
Modified residuei | 137 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 139 | PhosphoserineCombined sources | 1 | |
Modified residuei | 140 | PhosphoserineCombined sources | 1 | |
Cross-linki | 178 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 216 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 381 | PhosphoserineBy similarity | 1 | |
Modified residuei | 484 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P97310 |
jPOSTi | P97310 |
MaxQBi | P97310 |
PaxDbi | P97310 |
PeptideAtlasi | P97310 |
PRIDEi | P97310 |
PTM databases
iPTMneti | P97310 |
PhosphoSitePlusi | P97310 |
SwissPalmi | P97310 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000002870, Expressed in bone marrow and 276 other tissues |
ExpressionAtlasi | P97310, baseline and differential |
Genevisiblei | P97310, MM |
Interactioni
Subunit structurei
Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (By similarity).
Interacts with DBF4 (PubMed:10517317).
Interacts with KAT7 (PubMed:11278932). May interact with MCM10 (By similarity).
Component of the replisome complex composed of at least DONSON, MCM2, MCM7, PCNA and TICRR (By similarity).
Component of the CMG helicase complex, composed of the MCM2-7 complex, the GINS complex and CDC45 (By similarity).
By similarity2 PublicationsGO - Molecular functioni
- enzyme binding Source: BHF-UCL
- histone binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 201346, 18 interactors |
ComplexPortali | CPX-2941, MCM complex |
CORUMi | P97310 |
DIPi | DIP-33057N |
IntActi | P97310, 16 interactors |
MINTi | P97310 |
STRINGi | 10090.ENSMUSP00000061923 |
Miscellaneous databases
RNActi | P97310, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 473 – 680 | MCMAdd BLAST | 208 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 257 | Interaction with KAT71 PublicationAdd BLAST | 256 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 655 – 658 | Arginine finger | 4 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 329 – 355 | C4-typeSequence analysisAdd BLAST | 27 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG0477, Eukaryota |
GeneTreei | ENSGT01010000222249 |
HOGENOMi | CLU_000995_0_1_1 |
InParanoidi | P97310 |
OMAi | DCVKCGY |
OrthoDBi | 266497at2759 |
PhylomeDBi | P97310 |
TreeFami | TF300772 |
Family and domain databases
InterProi | View protein in InterPro IPR031327, MCM IPR008045, MCM2 IPR018525, MCM_CS IPR001208, MCM_dom IPR041562, MCM_lid IPR027925, MCM_N IPR033762, MCM_OB IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase |
PANTHERi | PTHR11630, PTHR11630, 1 hit PTHR11630:SF44, PTHR11630:SF44, 1 hit |
Pfami | View protein in Pfam PF00493, MCM, 1 hit PF12619, MCM2_N, 1 hit PF17855, MCM_lid, 1 hit PF14551, MCM_N, 1 hit PF17207, MCM_OB, 1 hit |
PRINTSi | PR01657, MCMFAMILY PR01658, MCMPROTEIN2 |
SMARTi | View protein in SMART SM00350, MCM, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00847, MCM_1, 1 hit PS50051, MCM_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MAESSESLSA SSPARQRRRI SDPLTSSPGR SSRRADALTS SPGRDLPPFE
60 70 80 90 100
DESEGLLGTE GPMEEEEDGE ELIGDGMERD YRPIPELDVY EAEGLALDDE
110 120 130 140 150
DVEELTASQR EAAERTMRQR DREAGRGLGR MRRGLLYDSS EEDEERPARK
160 170 180 190 200
RRHVERATED GEEDEEMIES IENLEDLKGH SVREWVSMAG PRLEIHHRFK
210 220 230 240 250
NFLRTHVDSH GHNVFKERIS DMCKENRESL VVNYEDLAAR EHVLAYFLPE
260 270 280 290 300
APAELLQIFD EAALEVVLAM YPKYDRITNH IHVRISHLPL VEELRSLRQL
310 320 330 340 350
HLNQLIRTSG VVTSCTGVLP QLSMVKYNCS KCNFVLGPFC QSQNQEVKPG
360 370 380 390 400
SCPECQSAGP FEINMEETIY QNYQRIRIQE SPGKVAAGRL PRSKDAILLA
410 420 430 440 450
DLVDSCKPGD EIELTGIYHN NYDGSLNTAN GFPVFATIIL ANHVAKKDNK
460 470 480 490 500
VAVGELTDED VKMITGLSKD QQIGEKIFAS IAPSIYGHED IKRGLALALF
510 520 530 540 550
GGEPKNPGGK HKVRGDINVL LCGDPGTAKS QFLKYIEKVS SRAIFTTGQG
560 570 580 590 600
ASAVGLTAYV QRHPVSREWT LEAGALVLAD RGVCLIDEFD KMNDQDRTSI
610 620 630 640 650
HEAMEQQSIS ISKAGIVTSL QARCTVIAAA NPIGGRYDPS LTFSENVDLT
660 670 680 690 700
EPIISRFDVL CVVRDTVDPV QDEMLARFVV GSHVRHHPSN KKDEGLTNGG
710 720 730 740 750
TLEPAMPNTY GVEPLPQEVL KKYIIYAKER VRPKLNQMDQ DKVARMYSDL
760 770 780 790 800
RKESMATGSI PITVRHIESM IRMAEAHARM HLRDYVMEDD VNMAIRVMME
810 820 830 840 850
SFIDTQKFSV MRSMRKTFAR YLSFRRDNND LLLFILKQLV AEQVTYQRNR
860 870 880 890 900
FGAQQDTIEI PEKDLMDKAR QINIHNLSAF YDSDLFKFNK FSRDLKRKLI
LQQF
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0N4SVY2 | A0A0N4SVY2_MOUSE | DNA replication licensing factor MC... | Mcm2 | 90 | Annotation score: | ||
A0A0N4SW20 | A0A0N4SW20_MOUSE | DNA replication licensing factor MC... | Mcm2 | 34 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 70 | E → G in AAC16250 (PubMed:9371513).Curated | 1 | |
Sequence conflicti | 135 | L → P in AAC16250 (PubMed:9371513).Curated | 1 | |
Sequence conflicti | 185 | W → R in BAA22148 (PubMed:9077461).Curated | 1 | |
Sequence conflicti | 271 | Y → H in AAC16250 (PubMed:9371513).Curated | 1 | |
Sequence conflicti | 506 | N → D in AAC36510 (PubMed:9798653).Curated | 1 | |
Sequence conflicti | 521 | L → S in AAC36510 (PubMed:9798653).Curated | 1 | |
Sequence conflicti | 648 | D → N in AAC36510 (PubMed:9798653).Curated | 1 | |
Sequence conflicti | 822 | L → I in AAC16250 (PubMed:9371513).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D86725 mRNA Translation: BAA22148.1 AF004105 mRNA Translation: AAC16250.1 AK129039 mRNA Translation: BAC97849.1 Different initiation. AK088156 mRNA Translation: BAC40178.1 BC055318 mRNA Translation: AAH55318.1 U89403 mRNA Translation: AAC36510.1 |
CCDSi | CCDS39554.1 |
PIRi | T10067 |
RefSeqi | NP_032590.2, NM_008564.2 |
Genome annotation databases
Ensembli | ENSMUST00000058011; ENSMUSP00000061923; ENSMUSG00000002870 |
GeneIDi | 17216 |
KEGGi | mmu:17216 |
UCSCi | uc009cvx.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D86725 mRNA Translation: BAA22148.1 AF004105 mRNA Translation: AAC16250.1 AK129039 mRNA Translation: BAC97849.1 Different initiation. AK088156 mRNA Translation: BAC40178.1 BC055318 mRNA Translation: AAH55318.1 U89403 mRNA Translation: AAC36510.1 |
CCDSi | CCDS39554.1 |
PIRi | T10067 |
RefSeqi | NP_032590.2, NM_008564.2 |
3D structure databases
SMRi | P97310 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201346, 18 interactors |
ComplexPortali | CPX-2941, MCM complex |
CORUMi | P97310 |
DIPi | DIP-33057N |
IntActi | P97310, 16 interactors |
MINTi | P97310 |
STRINGi | 10090.ENSMUSP00000061923 |
PTM databases
iPTMneti | P97310 |
PhosphoSitePlusi | P97310 |
SwissPalmi | P97310 |
Proteomic databases
EPDi | P97310 |
jPOSTi | P97310 |
MaxQBi | P97310 |
PaxDbi | P97310 |
PeptideAtlasi | P97310 |
PRIDEi | P97310 |
Protocols and materials databases
Antibodypediai | 4369, 987 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000058011; ENSMUSP00000061923; ENSMUSG00000002870 |
GeneIDi | 17216 |
KEGGi | mmu:17216 |
UCSCi | uc009cvx.2, mouse |
Organism-specific databases
CTDi | 4171 |
MGIi | MGI:105380, Mcm2 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG0477, Eukaryota |
GeneTreei | ENSGT01010000222249 |
HOGENOMi | CLU_000995_0_1_1 |
InParanoidi | P97310 |
OMAi | DCVKCGY |
OrthoDBi | 266497at2759 |
PhylomeDBi | P97310 |
TreeFami | TF300772 |
Enzyme and pathway databases
Reactomei | R-MMU-176187, Activation of ATR in response to replication stress R-MMU-68867, Assembly of the pre-replicative complex R-MMU-68949, Orc1 removal from chromatin R-MMU-68962, Activation of the pre-replicative complex R-MMU-69052, Switching of origins to a post-replicative state |
Miscellaneous databases
BioGRID-ORCSi | 17216, 6 hits in 18 CRISPR screens |
ChiTaRSi | Mcm2, mouse |
PROi | PR:P97310 |
RNActi | P97310, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000002870, Expressed in bone marrow and 276 other tissues |
ExpressionAtlasi | P97310, baseline and differential |
Genevisiblei | P97310, MM |
Family and domain databases
InterProi | View protein in InterPro IPR031327, MCM IPR008045, MCM2 IPR018525, MCM_CS IPR001208, MCM_dom IPR041562, MCM_lid IPR027925, MCM_N IPR033762, MCM_OB IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase |
PANTHERi | PTHR11630, PTHR11630, 1 hit PTHR11630:SF44, PTHR11630:SF44, 1 hit |
Pfami | View protein in Pfam PF00493, MCM, 1 hit PF12619, MCM2_N, 1 hit PF17855, MCM_lid, 1 hit PF14551, MCM_N, 1 hit PF17207, MCM_OB, 1 hit |
PRINTSi | PR01657, MCMFAMILY PR01658, MCMPROTEIN2 |
SMARTi | View protein in SMART SM00350, MCM, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00847, MCM_1, 1 hit PS50051, MCM_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MCM2_MOUSE | |
Accessioni | P97310Primary (citable) accession number: P97310 Secondary accession number(s): O08971, O89057, Q8C2R0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | April 13, 2004 | |
Last modified: | December 2, 2020 | |
This is version 195 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families