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UniProtKB - P97309 (MESP1_MOUSE)
Protein
Mesoderm posterior protein 1
Gene
Mesp1
Organism
Mus musculus (Mouse)
Status
Functioni
Transcription factor. Plays a role in the epithelialization of somitic mesoderm and in the development of cardiac mesoderm. Defines the rostrocaudal patterning of the somites by participating in distinct Notch pathways.
2 PublicationsGO - Molecular functioni
- cis-regulatory region sequence-specific DNA binding Source: BHF-UCL
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity Source: BHF-UCL
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- protein dimerization activity Source: InterPro
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- transcription cis-regulatory region binding Source: MGI
GO - Biological processi
- cardiac atrium formation Source: MGI
- cardiac cell fate determination Source: BHF-UCL
- cardiac muscle cell differentiation Source: MGI
- cardiac vascular smooth muscle cell differentiation Source: MGI
- cardiac ventricle formation Source: MGI
- cardioblast anterior-lateral migration Source: BHF-UCL
- cardioblast migration Source: MGI
- cardioblast migration to the midline involved in heart field formation Source: BHF-UCL
- embryonic heart tube morphogenesis Source: BHF-UCL
- endothelial cell differentiation Source: MGI
- gastrulation Source: BHF-UCL
- gene expression Source: MGI
- growth involved in heart morphogenesis Source: BHF-UCL
- heart looping Source: BHF-UCL
- heart morphogenesis Source: MGI
- lateral mesoderm development Source: BHF-UCL
- mesodermal cell migration Source: MGI
- mesoderm formation Source: GO_Central
- negative regulation of endodermal cell fate specification Source: BHF-UCL
- negative regulation of mesodermal cell fate specification Source: BHF-UCL
- negative regulation of transcription, DNA-templated Source: BHF-UCL
- neurogenesis Source: MGI
- Notch signaling pathway Source: UniProtKB-KW
- positive regulation of heart induction by negative regulation of canonical Wnt signaling pathway Source: BHF-UCL
- positive regulation of hepatocyte differentiation Source: BHF-UCL
- positive regulation of Notch signaling pathway Source: BHF-UCL
- positive regulation of Notch signaling pathway involved in heart induction Source: BHF-UCL
- positive regulation of striated muscle cell differentiation Source: BHF-UCL
- positive regulation of transcription, DNA-templated Source: BHF-UCL
- positive regulation of transcription by RNA polymerase II Source: BHF-UCL
- regulation of transcription by RNA polymerase II Source: GO_Central
- secondary heart field specification Source: BHF-UCL
- signal transduction involved in regulation of gene expression Source: MGI
- sinoatrial node cell differentiation Source: MGI
- sinus venosus morphogenesis Source: BHF-UCL
- somite rostral/caudal axis specification Source: GO_Central
Keywordsi
Molecular function | Developmental protein, DNA-binding |
Biological process | Notch signaling pathway, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Mesoderm posterior protein 1 |
Gene namesi | Name:Mesp1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:107785, Mesp1 |
VEuPathDBi | HostDB:ENSMUSG00000030544 |
Subcellular locationi
Nucleus
- Nucleus Curated
Nucleus
- nucleus Source: BHF-UCL
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Mice embryos exhibit defects in single heart tube formation, due to a delay in mesodermal migration. However, the cells eventually acquired migratory activity and gave rise to an abnormal heart tube. Mice lacking Mesp1 and Mesp2 die around 9.5 dpc. The major defect is the apparent lack of any mesodermal layer between endoderm and ectoderm and a defect in the migratory activity of mesodermal cells. It seems that a compensatory mechanism exists in which Mesp1 expression is up-regulated in the absence of Mesp2. Mesp1 may be involved in the rescue of somitogenesis in the null Mesp2 embryos.3 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000304405 | 1 – 243 | Mesoderm posterior protein 1Add BLAST | 243 |
Proteomic databases
PaxDbi | P97309 |
PRIDEi | P97309 |
PTM databases
PhosphoSitePlusi | P97309 |
Expressioni
Tissue specificityi
No expression was detected in adult tissues except the testis. Expression in the testis was regulated developmentally; expressed 2 weeks after birth, and increases, reaching the full expression level in mature testes.1 Publication
Developmental stagei
The earliest expression is detected at the junction of the epiblast and extraembryonic ectoderm, which is the initiation site for gastrulation. Around 6.5-6.75 dpc, expression becomes evident as gastrulation progresses. Expression does not spread over all mesodermal cells; it is seen only in a fraction of cells, particularly those in the early emergence group destined to become the extraembryonic mesoderm and the most posterior part of the tailbud. Expression remains for a while at the base of the allantois, and then spreads out to the lateral margins of the tail bud mesoderm. Expressed in the anterior presomitic mesoderm in a broad or thin stripe pattern. Expressed in the paraxial mesoderm during somitogenesis. Expression disappears before 8.5 dpc.4 Publications
Inductioni
Expression is induced by Notch signaling.
Gene expression databases
Bgeei | ENSMUSG00000030544, Expressed in testis and 84 other tissues |
Genevisiblei | P97309, MM |
Interactioni
GO - Molecular functioni
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000032760 |
Miscellaneous databases
RNActi | P97309, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 76 – 130 | bHLHPROSITE-ProRule annotationAdd BLAST | 55 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 86 | DisorderedSequence analysisAdd BLAST | 86 | |
Regioni | 204 – 228 | DisorderedSequence analysisAdd BLAST | 25 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 153 – 157 | CPLCP | 5 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 26 – 44 | Polar residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 204 – 226 | Polar residuesSequence analysisAdd BLAST | 23 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG4029, Eukaryota |
GeneTreei | ENSGT00530000063712 |
HOGENOMi | CLU_064749_1_0_1 |
InParanoidi | P97309 |
OMAi | PLSESWM |
OrthoDBi | 1072866at2759 |
PhylomeDBi | P97309 |
TreeFami | TF325707 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR040259, Mesogenin/MesP |
PANTHERi | PTHR20937, PTHR20937, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
i Sequence
Sequence statusi: Complete.
P97309-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAQPLCEPRS ESWILSPAGR QPPMPSDGNS VCSPAWSSDP WDGAQASSPA
60 70 80 90 100
PPCARPARRA GTPGRRGTHG SRLGSGQRQS ASEREKLRMR TLARALHELR
110 120 130 140 150
RFLPPSVAPT GQNLTKIETL RLAIRYIGHL SAVLGLSEDN LRRQRHAVSP
160 170 180 190 200
RGCPLCPDSD LAQSQSLGPR LSPAVCSGVS WGSPPAYPRP RVAAESWDPS
210 220 230 240
FLYAETASQE RQEMEPSPSS PLFSSDMLAL LETWTPPQEW PPA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 170 | R → G in BAA12041 (PubMed:8787751).Curated | 1 | |
Sequence conflicti | 202 | L → Q in BAA12041 (PubMed:8787751).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D83674 mRNA Translation: BAA12041.1 BC125505 mRNA Translation: AAI25506.1 BC125507 mRNA Translation: AAI25508.1 |
CCDSi | CCDS21387.1 |
RefSeqi | NP_032614.2, NM_008588.2 |
Genome annotation databases
Ensembli | ENSMUST00000032760; ENSMUSP00000032760; ENSMUSG00000030544 |
GeneIDi | 17292 |
KEGGi | mmu:17292 |
UCSCi | uc009hzb.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D83674 mRNA Translation: BAA12041.1 BC125505 mRNA Translation: AAI25506.1 BC125507 mRNA Translation: AAI25508.1 |
CCDSi | CCDS21387.1 |
RefSeqi | NP_032614.2, NM_008588.2 |
3D structure databases
AlphaFoldDBi | P97309 |
SMRi | P97309 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000032760 |
PTM databases
PhosphoSitePlusi | P97309 |
Proteomic databases
PaxDbi | P97309 |
PRIDEi | P97309 |
Protocols and materials databases
Antibodypediai | 28662, 327 antibodies from 30 providers |
DNASUi | 17292 |
Genome annotation databases
Ensembli | ENSMUST00000032760; ENSMUSP00000032760; ENSMUSG00000030544 |
GeneIDi | 17292 |
KEGGi | mmu:17292 |
UCSCi | uc009hzb.2, mouse |
Organism-specific databases
CTDi | 55897 |
MGIi | MGI:107785, Mesp1 |
VEuPathDBi | HostDB:ENSMUSG00000030544 |
Phylogenomic databases
eggNOGi | KOG4029, Eukaryota |
GeneTreei | ENSGT00530000063712 |
HOGENOMi | CLU_064749_1_0_1 |
InParanoidi | P97309 |
OMAi | PLSESWM |
OrthoDBi | 1072866at2759 |
PhylomeDBi | P97309 |
TreeFami | TF325707 |
Miscellaneous databases
BioGRID-ORCSi | 17292, 1 hit in 73 CRISPR screens |
ChiTaRSi | Mesp1, mouse |
PROi | PR:P97309 |
RNActi | P97309, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000030544, Expressed in testis and 84 other tissues |
Genevisiblei | P97309, MM |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR040259, Mesogenin/MesP |
PANTHERi | PTHR20937, PTHR20937, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MESP1_MOUSE | |
Accessioni | P97309Primary (citable) accession number: P97309 Secondary accession number(s): Q059W2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 11, 2007 |
Last sequence update: | September 11, 2007 | |
Last modified: | May 25, 2022 | |
This is version 130 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot