UniProtKB - P97287 (MCL1_MOUSE)
Induced myeloid leukemia cell differentiation protein Mcl-1 homolog
Mcl1
Functioni
Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 2 has antiapoptotic activity.
2 PublicationsGO - Molecular functioni
- BH3 domain binding Source: UniProtKB
- BH domain binding Source: MGI
- protein-containing complex binding Source: MGI
- protein dimerization activity Source: MGI
- protein heterodimerization activity Source: MGI
- protein homodimerization activity Source: GO_Central
GO - Biological processi
- cell differentiation Source: UniProtKB-KW
- extrinsic apoptotic signaling pathway in absence of ligand Source: UniProtKB
- intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
- negative regulation of anoikis Source: MGI
- negative regulation of apoptotic process Source: UniProtKB
- negative regulation of autophagy Source: MGI
- negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
- negative regulation of intrinsic apoptotic signaling pathway Source: GO_Central
- positive regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway Source: ParkinsonsUK-UCL
- regulation of response to DNA damage stimulus Source: MGI
- response to cytokine Source: MGI
Keywordsi
Molecular function | Developmental protein |
Biological process | Apoptosis, Differentiation |
Names & Taxonomyi
Protein namesi | Recommended name: Induced myeloid leukemia cell differentiation protein Mcl-1 homologAlternative name(s): Bcl-2-related protein EAT/mcl1 |
Gene namesi | Name:Mcl1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:101769, Mcl1 |
VEuPathDBi | HostDB:ENSMUSG00000038612 |
Subcellular locationi
Nucleus
- nucleoplasm By similarity
Mitochondrion
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- Membrane By similarity; Single-pass membrane protein By similarity
Note: Cytoplasmic, associated with mitochondria.
Cytosol
- cytosol Source: UniProtKB
Mitochondrion
- mitochondrial outer membrane Source: GO_Central
- mitochondrion Source: ParkinsonsUK-UCL
Nucleus
- nucleoplasm Source: UniProtKB-SubCell
- nucleus Source: MGI
Other locations
- Bcl-2 family protein complex Source: MGI
- cytoplasm Source: MGI
- integral component of membrane Source: UniProtKB-KW
- membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 308 – 330 | HelicalSequence analysisAdd BLAST | 23 |
Keywords - Cellular componenti
Cytoplasm, Membrane, Mitochondrion, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000143081 | 1 – 331 | Induced myeloid leukemia cell differentiation protein Mcl-1 homologAdd BLAST | 331 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 102 | PhosphoserineBy similarity | 1 | |
Cross-linki | 117 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 140 | Phosphoserine; by GSK3-alpha and GSK3-beta1 Publication | 1 | |
Modified residuei | 143 | PhosphoserineBy similarity | 1 | |
Modified residuei | 144 | Phosphothreonine; by MAPKBy similarity | 1 | |
Cross-linki | 175 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 178 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 108 – 109 | Cleavage; by caspase-3By similarity | 2 | |
Sitei | 138 – 139 | Cleavage; by caspase-3By similarity | 2 |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P97287 |
jPOSTi | P97287 |
MaxQBi | P97287 |
PaxDbi | P97287 |
PeptideAtlasi | P97287 |
PRIDEi | P97287 |
ProteomicsDBi | 252747 [P97287-1] 252748 [P97287-2] |
PTM databases
iPTMneti | P97287 |
PhosphoSitePlusi | P97287 |
Expressioni
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000038612, Expressed in ear vesicle and 323 other tissues |
Genevisiblei | P97287, MM |
Interactioni
Subunit structurei
Interacts with HIF3A isoform 2 (via C-terminus domain) (PubMed:21546903).
Interacts with BAD, BOK, BIK, BAX, BAK1, and TPT1.
Interacts with BBC3, BMF and PMAIP1 (PubMed:15550399, PubMed:18589438).
Interacts with BOP.
Interacts with BCL2L11; this interaction may sequester BCL2L11 and prevent its pro-apoptotic activity (PubMed:16543145, PubMed:27013495, PubMed:15550399).
Interacts with GIMAP5 and HSPA8/HSC70; the interaction between HSPA8 and MCL1 is impaired in the absence of GIMAP5 (PubMed:21502331).
6 PublicationsBinary interactionsi
P97287
GO - Molecular functioni
- BH3 domain binding Source: UniProtKB
- BH domain binding Source: MGI
- protein dimerization activity Source: MGI
- protein heterodimerization activity Source: MGI
- protein homodimerization activity Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 201344, 19 interactors |
ComplexPortali | CPX-305, MCL1:PMAIP1 complex CPX-629, MCL-1-BIM complex |
CORUMi | P97287 |
DIPi | DIP-33343N |
ELMi | P97287 |
IntActi | P97287, 18 interactors |
MINTi | P97287 |
STRINGi | 10090.ENSMUSP00000044048 |
Chemistry databases
BindingDBi | P97287 |
Miscellaneous databases
RNActi | P97287, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P97287 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P97287 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 85 – 156 | PEST-likeBy similarityAdd BLAST | 72 | |
Regioni | 130 – 154 | DisorderedSequence analysisAdd BLAST | 25 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 190 – 204 | BH3Add BLAST | 15 | |
Motifi | 234 – 253 | BH1Add BLAST | 20 | |
Motifi | 285 – 300 | BH2Add BLAST | 16 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4728, Eukaryota |
GeneTreei | ENSGT01040000240472 |
HOGENOMi | CLU_046711_0_0_1 |
InParanoidi | P97287 |
OMAi | TKRDWLI |
OrthoDBi | 1218929at2759 |
PhylomeDBi | P97287 |
TreeFami | TF315834 |
Family and domain databases
CDDi | cd06845, Bcl-2_like, 1 hit |
Gene3Di | 1.10.437.10, 1 hit |
InterProi | View protein in InterPro IPR013281, Apop_reg_Mc1 IPR036834, Bcl-2-like_sf IPR026298, Bcl-2_fam IPR002475, Bcl2-like IPR020717, Bcl2_BH1_motif_CS IPR020726, Bcl2_BH2_motif_CS IPR020728, Bcl2_BH3_motif_CS |
PANTHERi | PTHR11256, PTHR11256, 1 hit PTHR11256:SF46, PTHR11256:SF46, 1 hit |
Pfami | View protein in Pfam PF00452, Bcl-2, 1 hit |
PRINTSi | PR01866, APOPREGMCL1 PR01862, BCL2FAMILY |
SMARTi | View protein in SMART SM00337, BCL, 1 hit |
SUPFAMi | SSF56854, SSF56854, 1 hit |
PROSITEi | View protein in PROSITE PS50062, BCL2_FAMILY, 1 hit PS01080, BH1, 1 hit PS01258, BH2, 1 hit PS01259, BH3, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MFGLRRNAVI GLNLYCGGAS LGAGGGSPAG ARLVAEEAKA RREGGGEAAL
60 70 80 90 100
LPGARVVARP PPVGAEDPDV TASAERRLHK SPGLLAVPPE EMAASAAAAI
110 120 130 140 150
VSPEEELDGC EPEAIGKRPA VLPLLERVSE AAKSSGADGS LPSTPPPPEE
160 170 180 190 200
EEDDLYRQSL EIISRYLREQ ATGSKDSKPL GEAGAAGRRA LETLRRVGDG
210 220 230 240 250
VQRNHETAFQ GMLRKLDIKN EGDVKSFSRV MVHVFKDGVT NWGRIVTLIS
260 270 280 290 300
FGAFVAKHLK SVNQESFIEP LAETITDVLV RTKRDWLVKQ RGWDGFVEFF
310 320 330
HVQDLEGGIR NVLLAFAGVA GVGAGLAYLI R
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_046443 | 18 – 63 | Missing in isoform 2. 1 PublicationAdd BLAST | 46 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U35623 mRNA Translation: AAC31790.1 GU182318 mRNA Translation: ACZ54910.1 AK010424 mRNA Translation: BAB26927.1 AK159504 mRNA Translation: BAE35137.1 AK160594 mRNA Translation: BAE35901.1 AC092479 Genomic DNA No translation available. FO082281 Genomic DNA No translation available. BC003839 mRNA Translation: AAH03839.1 BC005427 mRNA Translation: AAH05427.1 BC021638 mRNA Translation: AAH21638.1 AF063886 Genomic DNA Translation: AAC27929.1 |
CCDSi | CCDS57236.1 [P97287-1] |
RefSeqi | NP_032588.1, NM_008562.3 [P97287-1] |
Genome annotation databases
Ensembli | ENSMUST00000037947; ENSMUSP00000044048; ENSMUSG00000038612 [P97287-1] ENSMUST00000178686; ENSMUSP00000135915; ENSMUSG00000038612 [P97287-2] |
GeneIDi | 17210 |
KEGGi | mmu:17210 |
UCSCi | uc029unp.1, mouse [P97287-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U35623 mRNA Translation: AAC31790.1 GU182318 mRNA Translation: ACZ54910.1 AK010424 mRNA Translation: BAB26927.1 AK159504 mRNA Translation: BAE35137.1 AK160594 mRNA Translation: BAE35901.1 AC092479 Genomic DNA No translation available. FO082281 Genomic DNA No translation available. BC003839 mRNA Translation: AAH03839.1 BC005427 mRNA Translation: AAH05427.1 BC021638 mRNA Translation: AAH21638.1 AF063886 Genomic DNA Translation: AAC27929.1 |
CCDSi | CCDS57236.1 [P97287-1] |
RefSeqi | NP_032588.1, NM_008562.3 [P97287-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1WSX | NMR | - | A | 152-308 | [»] | |
2JM6 | NMR | - | B | 152-308 | [»] | |
2NL9 | X-ray | 1.55 | A | 152-189 | [»] | |
2NLA | X-ray | 2.80 | A | 152-189 | [»] | |
2ROC | NMR | - | A | 152-308 | [»] | |
2ROD | NMR | - | A | 152-308 | [»] | |
3D7V | X-ray | 2.03 | A | 152-189 | [»] | |
3IO9 | X-ray | 2.40 | A | 152-189 | [»] | |
4BPI | X-ray | 1.98 | A | 152-189 | [»] | |
4BPJ | X-ray | 1.60 | A | 152-189 | [»] | |
4G35 | X-ray | 2.00 | A | 152-308 | [»] | |
5KU9 | X-ray | 2.20 | A/B | 152-308 | [»] | |
5MES | X-ray | 2.24 | A | 153-221 | [»] | |
5MEV | X-ray | 2.94 | A | 153-221 | [»] | |
5VX2 | X-ray | 1.85 | A/C | 152-221 | [»] | |
6DM8 | X-ray | 2.70 | A/B/C/D/E/F/G/H | 154-302 | [»] | |
SMRi | P97287 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 201344, 19 interactors |
ComplexPortali | CPX-305, MCL1:PMAIP1 complex CPX-629, MCL-1-BIM complex |
CORUMi | P97287 |
DIPi | DIP-33343N |
ELMi | P97287 |
IntActi | P97287, 18 interactors |
MINTi | P97287 |
STRINGi | 10090.ENSMUSP00000044048 |
Chemistry databases
BindingDBi | P97287 |
ChEMBLi | CHEMBL5768 |
PTM databases
iPTMneti | P97287 |
PhosphoSitePlusi | P97287 |
Proteomic databases
EPDi | P97287 |
jPOSTi | P97287 |
MaxQBi | P97287 |
PaxDbi | P97287 |
PeptideAtlasi | P97287 |
PRIDEi | P97287 |
ProteomicsDBi | 252747 [P97287-1] 252748 [P97287-2] |
Protocols and materials databases
ABCDi | P97287, 1 sequenced antibody |
Antibodypediai | 1508, 1426 antibodies from 50 providers |
DNASUi | 17210 |
Genome annotation databases
Ensembli | ENSMUST00000037947; ENSMUSP00000044048; ENSMUSG00000038612 [P97287-1] ENSMUST00000178686; ENSMUSP00000135915; ENSMUSG00000038612 [P97287-2] |
GeneIDi | 17210 |
KEGGi | mmu:17210 |
UCSCi | uc029unp.1, mouse [P97287-1] |
Organism-specific databases
CTDi | 4170 |
MGIi | MGI:101769, Mcl1 |
VEuPathDBi | HostDB:ENSMUSG00000038612 |
Phylogenomic databases
eggNOGi | KOG4728, Eukaryota |
GeneTreei | ENSGT01040000240472 |
HOGENOMi | CLU_046711_0_0_1 |
InParanoidi | P97287 |
OMAi | TKRDWLI |
OrthoDBi | 1218929at2759 |
PhylomeDBi | P97287 |
TreeFami | TF315834 |
Miscellaneous databases
BioGRID-ORCSi | 17210, 21 hits in 66 CRISPR screens |
ChiTaRSi | Mcl1, mouse |
EvolutionaryTracei | P97287 |
PROi | PR:P97287 |
RNActi | P97287, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000038612, Expressed in ear vesicle and 323 other tissues |
Genevisiblei | P97287, MM |
Family and domain databases
CDDi | cd06845, Bcl-2_like, 1 hit |
Gene3Di | 1.10.437.10, 1 hit |
InterProi | View protein in InterPro IPR013281, Apop_reg_Mc1 IPR036834, Bcl-2-like_sf IPR026298, Bcl-2_fam IPR002475, Bcl2-like IPR020717, Bcl2_BH1_motif_CS IPR020726, Bcl2_BH2_motif_CS IPR020728, Bcl2_BH3_motif_CS |
PANTHERi | PTHR11256, PTHR11256, 1 hit PTHR11256:SF46, PTHR11256:SF46, 1 hit |
Pfami | View protein in Pfam PF00452, Bcl-2, 1 hit |
PRINTSi | PR01866, APOPREGMCL1 PR01862, BCL2FAMILY |
SMARTi | View protein in SMART SM00337, BCL, 1 hit |
SUPFAMi | SSF56854, SSF56854, 1 hit |
PROSITEi | View protein in PROSITE PS50062, BCL2_FAMILY, 1 hit PS01080, BH1, 1 hit PS01258, BH2, 1 hit PS01259, BH3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MCL1_MOUSE | |
Accessioni | P97287Primary (citable) accession number: P97287 Secondary accession number(s): D2K6L9 Q9CRI4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 6, 2005 |
Last sequence update: | November 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 180 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families