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Protein

Epoxyqueuosine reductase

Gene

queG

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of epoxyqueuosine (oQ) to queuosine (Q), which is a hypermodified base found in the wobble positions of tRNA(Asp), tRNA(Asn), tRNA(His) and tRNA(Tyr).UniRule annotation1 Publication

Catalytic activityi

Queuosine(34) in tRNA + acceptor + H2O = epoxyqueuosine(34) in tRNA + reduced acceptor.UniRule annotation1 Publication

Enzyme regulationi

Stimulated by cobalamine. Activity requires inclusion of a reductant and a redox mediator.1 Publication

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi188Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi191Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi194Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi198Iron-sulfur 2UniRule annotation1
Metal bindingi240Iron-sulfur 2UniRule annotation1
Metal bindingi243Iron-sulfur 2UniRule annotation1
Metal bindingi247Iron-sulfur 1 (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processQueuosine biosynthesis, tRNA processing
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU08910-MONOMER
UniPathwayiUPA00392

Names & Taxonomyi

Protein namesi
Recommended name:
Epoxyqueuosine reductaseUniRule annotation (EC:1.17.99.6UniRule annotation1 Publication)
Alternative name(s):
Queuosine biosynthesis protein QueGUniRule annotation
Gene namesi
Name:queGUniRule annotation
Synonyms:ygaP, yhbA
Ordered Locus Names:BSU08910
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003606481 – 386Epoxyqueuosine reductaseAdd BLAST386

Proteomic databases

PaxDbiP97030
PRIDEiP97030

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100004938

Structurei

Secondary structure

1386
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 16Combined sources14
Beta strandi20 – 26Combined sources7
Helixi31 – 43Combined sources13
Helixi54 – 58Combined sources5
Helixi60 – 63Combined sources4
Beta strandi69 – 76Combined sources8
Beta strandi89 – 91Combined sources3
Helixi98 – 100Combined sources3
Beta strandi101 – 103Combined sources3
Helixi105 – 121Combined sources17
Beta strandi129 – 138Combined sources10
Helixi140 – 146Combined sources7
Beta strandi158 – 160Combined sources3
Turni161 – 163Combined sources3
Beta strandi167 – 175Combined sources9
Helixi193 – 197Combined sources5
Beta strandi203 – 205Combined sources3
Helixi211 – 213Combined sources3
Helixi215 – 219Combined sources5
Helixi227 – 230Combined sources4
Beta strandi236 – 238Combined sources3
Helixi242 – 245Combined sources4
Helixi248 – 250Combined sources3
Helixi259 – 261Combined sources3
Helixi265 – 268Combined sources4
Helixi272 – 275Combined sources4
Helixi280 – 287Combined sources8
Helixi291 – 293Combined sources3
Helixi298 – 310Combined sources13
Helixi314 – 316Combined sources3
Helixi317 – 326Combined sources10
Helixi330 – 343Combined sources14
Helixi346 – 348Combined sources3
Helixi349 – 356Combined sources8
Helixi362 – 382Combined sources21

3D structure databases

ProteinModelPortaliP97030
SMRiP97030
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini178 – 2084Fe-4S ferredoxin-typeUniRule annotationAdd BLAST31
Repeati333 – 357HEAT-like PBS-typeAdd BLAST25

Sequence similaritiesi

Belongs to the QueG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EH2 Bacteria
COG1600 LUCA
HOGENOMiHOG000272645
InParanoidiP97030
KOiK18979
OMAiVDGSKCI
PhylomeDBiP97030

Family and domain databases

Gene3Di1.25.10.10, 1 hit
HAMAPiMF_00916 QueG, 1 hit
InterProiView protein in InterPro
IPR017896 4Fe4S_Fe-S-bd
IPR017900 4Fe4S_Fe_S_CS
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR013542 DUF1730
IPR004155 PBS_lyase_HEAT
IPR004453 QueG
PANTHERiPTHR30002 PTHR30002, 1 hit
PfamiView protein in Pfam
PF08331 DUF1730, 1 hit
SMARTiView protein in SMART
SM00567 EZ_HEAT, 2 hits
SUPFAMiSSF48371 SSF48371, 1 hit
TIGRFAMsiTIGR00276 TIGR00276, 1 hit
PROSITEiView protein in PROSITE
PS00198 4FE4S_FER_1, 1 hit
PS51379 4FE4S_FER_2, 1 hit

Sequencei

Sequence statusi: Complete.

P97030-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVYQLKEEL IEYAKSIGVD KIGFTTADTF DSLKDRLILQ ESLGYLSGFE
60 70 80 90 100
EPDIEKRVTP KLLLPKAKSI VAIALAYPSR MKDAPRSTRT ERRGIFCRAS
110 120 130 140 150
WGKDYHDVLR EKLDLLEDFL KSKHEDIRTK SMVDTGELSD RAVAERAGIG
160 170 180 190 200
FSAKNCMITT PEYGSYVYLA EMITNIPFEP DVPIEDMCGS CTKCLDACPT
210 220 230 240 250
GALVNPGQLN AQRCISFLTQ TKGFLPDEFR TKIGNRLYGC DTCQTVCPLN
260 270 280 290 300
KGKDFHLHPE MEPDPEIAKP LLKPLLAISN REFKEKFGHV SGSWRGKKPI
310 320 330 340 350
QRNAILALAH FKDASALPEL TELMHKDPRP VIRGTAAWAI GKIGDPAYAE
360 370 380
ELEKALEKEK DEEAKLEIEK GIELLKASGM TKQGLS
Length:386
Mass (Da):43,142
Last modified:April 14, 2009 - v2
Checksum:i56E5477D0D01CB30
GO

Sequence cautioni

The sequence CAB07527 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z93102 Genomic DNA Translation: CAB07527.1 Different initiation.
AL009126 Genomic DNA Translation: CAB12719.2
PIRiE69820
RefSeqiNP_388772.2, NC_000964.3
WP_003245522.1, NZ_JNCM01000035.1

Genome annotation databases

EnsemblBacteriaiCAB12719; CAB12719; BSU08910
GeneIDi939243
KEGGibsu:BSU08910
PATRICifig|224308.179.peg.962

Similar proteinsi

Entry informationi

Entry nameiQUEG_BACSU
AccessioniPrimary (citable) accession number: P97030
Secondary accession number(s): Q796Y4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: April 14, 2009
Last modified: May 23, 2018
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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