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Protein

CoB--CoM heterodisulfide reductase iron-sulfur subunit A 2

Gene

hdrA2

Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B).By similarity

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterPROSITE-ProRule annotationNote: Binds 4 [4Fe-4S] clusters per subunit.PROSITE-ProRule annotation
  • FADBy similarity

Pathwayi: coenzyme M-coenzyme B heterodisulfide reduction

This protein is involved in step 1 of the subpathway that synthesizes coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. CoB--CoM heterodisulfide reductase iron-sulfur subunit A 1 (hdrA1), CoB--CoM heterodisulfide reductase subunit B (hdrB), CoB--CoM heterodisulfide reductase iron-sulfur subunit C (hdrC), CoB--CoM heterodisulfide reductase iron-sulfur subunit A 2 (hdrA2)
This subpathway is part of the pathway coenzyme M-coenzyme B heterodisulfide reduction, which is itself part of Cofactor metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide, the pathway coenzyme M-coenzyme B heterodisulfide reduction and in Cofactor metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi248Iron-sulfur 1 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi251Iron-sulfur 1 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi254Iron-sulfur 1 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi258Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi295Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi298Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi301Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi305Iron-sulfur 1 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi586Iron-sulfur 3 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi589Iron-sulfur 3 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi592Iron-sulfur 3 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi596Iron-sulfur 4 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi619Iron-sulfur 4 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi622Iron-sulfur 4 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi625Iron-sulfur 4 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi629Iron-sulfur 3 (4Fe-4S)PROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi152 – 175FADSequence analysisAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processMethanogenesis
Ligand4Fe-4S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00647; UER00700

Names & Taxonomyi

Protein namesi
Recommended name:
CoB--CoM heterodisulfide reductase iron-sulfur subunit A 2Curated (EC:1.8.98.-Curated)
Gene namesi
Name:hdrA2
Synonyms:hdrA_2
Ordered Locus Names:MK0265
OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Taxonomic identifieri190192 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
Proteomesi
  • UP000001826 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001500591 – 656CoB--CoM heterodisulfide reductase iron-sulfur subunit A 2Add BLAST656

Proteomic databases

PRIDEiP96801

Interactioni

Subunit structurei

The ferredoxin:CoB-CoM heterodisulfide reductase is composed of three subunits; HdrA, HdrB and HdrC.By similarity

Protein-protein interaction databases

STRINGi190192.MK0265

Structurei

3D structure databases

ProteinModelPortaliP96801
SMRiP96801
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini238 – 2694Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST32
Domaini286 – 3154Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST30
Domaini577 – 6064Fe-4S ferredoxin-type 3PROSITE-ProRule annotationAdd BLAST30
Domaini610 – 6394Fe-4S ferredoxin-type 4PROSITE-ProRule annotationAdd BLAST30

Sequence similaritiesi

Belongs to the HdrA family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiarCOG02235 Archaea
COG1148 LUCA
HOGENOMiHOG000230698
KOiK03388
OMAiTAKHAML
OrthoDBiPOG093Z04QZ

Family and domain databases

Gene3Di3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR017896 4Fe4S_Fe-S-bd
IPR017900 4Fe4S_Fe_S_CS
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
PfamiView protein in Pfam
PF12831 FAD_oxidored, 1 hit
PF12838 Fer4_7, 1 hit
PF13187 Fer4_9, 1 hit
PF07992 Pyr_redox_2, 1 hit
SUPFAMiSSF51905 SSF51905, 1 hit
PROSITEiView protein in PROSITE
PS00198 4FE4S_FER_1, 3 hits
PS51379 4FE4S_FER_2, 4 hits

Sequencei

Sequence statusi: Complete.

P96801-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEEEPRIGV YVCHCGVNIA GVVDVKEVAE FAKTLKNVVV ARDYKYVCSD
60 70 80 90 100
PGQEIIQRDI EKYDLNRVVV AACSPRLHEP TFRRCVEEAG LNPYCFEMAN
110 120 130 140 150
IREHCSWVHM DDPARATEKA KDLVRMAVAK ARLLESLETI KVDVTDRALV
160 170 180 190 200
IGGGVSGIQA ALDLADMGFE VILVEKEPSI GGRMAQLDKT FPTNDCSICI
210 220 230 240 250
LAPKMVDVSK HPNIKMYTYA EVVEVDGYVG NFTVKIEKKP RYVDEDACTG
260 270 280 290 300
CGACAEVCPI EVPNEFDEGL GMRKAIYKPF PQAVPSVFTI DEEHCIRCGL
310 320 330 340 350
CEEVCDADAI DFDQEPEIVE EEVGAIICAI GYDTCDPTER EEYGYGVYDN
360 370 380 390 400
VITSIELERL INASGPTGGK VVRPSDGKKP KRIAFIQCVG SRDPHRTNPY
410 420 430 440 450
CSNVCCMYAM KLAQLIREKY PETQIDIYYM DVRAFGKGYE EYYERSQKQY
460 470 480 490 500
GIRFIRGRPA EIVEDPETKN LIVRAEDTLL GDVVEREYDL VVLSVGMVPR
510 520 530 540 550
DSADVIQEVL SISRSPDGFF MEAHPKLRPV DTAIDGIFLA GACQGPKDIP
560 570 580 590 600
SSVAQGSAAA ARAATALAAG EVAVEPIVSE VDEEICGGCG TCVELCPYGA
610 620 630 640 650
IELVEKDGKL VAEVTAALCK GCGTCAAACP SGAMEQNHFK TEQLYKQIEG

AFRDPA
Length:656
Mass (Da):71,925
Last modified:May 1, 1997 - v1
Checksum:iC7CF218545ACEA7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09871 Genomic DNA Translation: CAA70999.1
AE009439 Genomic DNA Translation: AAM01482.1

Genome annotation databases

EnsemblBacteriaiAAM01482; AAM01482; MK0265
KEGGimka:MK0265
PATRICifig|190192.8.peg.268

Similar proteinsi

Entry informationi

Entry nameiHDRA2_METKA
AccessioniPrimary (citable) accession number: P96801
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: May 1, 1997
Last modified: May 23, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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