UniProtKB - P96590 (MUTT_BACSU)
Protein
Putative 8-oxo-dGTP diphosphatase
Gene
mutT
Organism
Bacillus subtilis (strain 168)
Status
Functioni
May be involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine, 8-oxo-dGTP) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite dA and dC residues of template DNA with almost equal efficiency thus leading to A.T to G.C transversions. MutT specifically degrades 8-oxo-dGTP to the monophosphate (By similarity). Functions, in conjunction with ytkD, to protect vegetatively growing cells from DNA-damaging agents such as H2O2 or t-BHP (t-butylhydroperoxide). The 2 proteins do not however protect spores.By similarity
Catalytic activityi
- EC:3.6.1.55
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 48 | Magnesium or manganeseBy similarity | 1 | |
Metal bindingi | 52 | Magnesium or manganeseBy similarity | 1 |
GO - Molecular functioni
- hydrolase activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- DNA repair Source: UniProtKB-KW
- DNA replication Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, Mutator protein |
Biological process | DNA damage, DNA repair, DNA replication |
Ligand | Magnesium, Manganese, Metal-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU04330-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Putative 8-oxo-dGTP diphosphatase (EC:3.6.1.55)Short name: 8-oxo-dGTPase Alternative name(s): 7,8-dihydro-8-oxoguanine-triphosphatase Mutator protein MutT dGTP pyrophosphohydrolase |
Gene namesi | Name:mutT Ordered Locus Names:BSU04330 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Growing cells lacking this gene have an unchanged spontaneous mutation frequency, even in triple mutT/yjhB/yvcI disruptions (PubMed:12483591), however they are more sensitive to DNA-damaging agents such as H2O2 or t-BHP (PubMed:16513759). A double ytkD/mutT disruption has a greater mutation frequency than the ytkD disruption, as well as an increased sensitivity to DNA-damaging agents (PubMed:16513759). These properties are also seen in stationary phase cells (PubMed:19011023).3 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000379972 | 1 – 149 | Putative 8-oxo-dGTP diphosphataseAdd BLAST | 149 |
Proteomic databases
PaxDbi | P96590 |
PRIDEi | P96590 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 123 | Nudix hydrolasePROSITE-ProRule annotationAdd BLAST | 123 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 33 – 54 | Nudix boxAdd BLAST | 22 |
Sequence similaritiesi
Belongs to the Nudix hydrolase family.Curated
Phylogenomic databases
eggNOGi | COG0494 LUCA |
HOGENOMi | HOG000261967 |
InParanoidi | P96590 |
KOi | K03574 |
OMAi | YDIHPSM |
PhylomeDBi | P96590 |
Family and domain databases
InterProi | View protein in InterPro IPR020476 Nudix_hydrolase IPR015797 NUDIX_hydrolase-like_dom_sf IPR020084 NUDIX_hydrolase_CS IPR000086 NUDIX_hydrolase_dom |
Pfami | View protein in Pfam PF00293 NUDIX, 1 hit |
PRINTSi | PR00502 NUDIXFAMILY |
SUPFAMi | SSF55811 SSF55811, 1 hit |
PROSITEi | View protein in PROSITE PS51462 NUDIX, 1 hit PS00893 NUDIX_BOX, 1 hit |
i Sequence
Sequence statusi: Complete.
P96590-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MYTQGAFVIV LNESQQILLV KRKDVPLWDL PGGRVDPGES AEEAAVREIL
60 70 80 90 100
EETGYNAALS AKIGVYQRPK FQDEQHLFFG SITGGQAMAD GTETAGLKWV
110 120 130 140
SPGRLPLFMV PNRKRQINDF KNGAQDVNVT VKDSGLLAAI DLLKRRLGK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB001488 Genomic DNA Translation: BAA19270.1 AL009126 Genomic DNA Translation: CAB12240.1 |
PIRi | D69663 |
RefSeqi | NP_388314.1, NC_000964.3 WP_003246544.1, NZ_JNCM01000031.1 |
Genome annotation databases
EnsemblBacteriai | CAB12240; CAB12240; BSU04330 |
GeneIDi | 938242 |
KEGGi | bsu:BSU04330 |
PATRICi | fig|224308.179.peg.459 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB001488 Genomic DNA Translation: BAA19270.1 AL009126 Genomic DNA Translation: CAB12240.1 |
PIRi | D69663 |
RefSeqi | NP_388314.1, NC_000964.3 WP_003246544.1, NZ_JNCM01000031.1 |
3D structure databases
SMRi | P96590 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU04330 |
Proteomic databases
PaxDbi | P96590 |
PRIDEi | P96590 |
Genome annotation databases
EnsemblBacteriai | CAB12240; CAB12240; BSU04330 |
GeneIDi | 938242 |
KEGGi | bsu:BSU04330 |
PATRICi | fig|224308.179.peg.459 |
Phylogenomic databases
eggNOGi | COG0494 LUCA |
HOGENOMi | HOG000261967 |
InParanoidi | P96590 |
KOi | K03574 |
OMAi | YDIHPSM |
PhylomeDBi | P96590 |
Enzyme and pathway databases
BioCyci | BSUB:BSU04330-MONOMER |
Family and domain databases
InterProi | View protein in InterPro IPR020476 Nudix_hydrolase IPR015797 NUDIX_hydrolase-like_dom_sf IPR020084 NUDIX_hydrolase_CS IPR000086 NUDIX_hydrolase_dom |
Pfami | View protein in Pfam PF00293 NUDIX, 1 hit |
PRINTSi | PR00502 NUDIXFAMILY |
SUPFAMi | SSF55811 SSF55811, 1 hit |
PROSITEi | View protein in PROSITE PS51462 NUDIX, 1 hit PS00893 NUDIX_BOX, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MUTT_BACSU | |
Accessioni | P96590Primary (citable) accession number: P96590 Secondary accession number(s): Q797M2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 28, 2009 |
Last sequence update: | May 1, 1997 | |
Last modified: | October 16, 2019 | |
This is version 110 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Bacillus subtilis
Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList