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Entry version 102 (02 Jun 2021)
Sequence version 1 (01 May 1997)
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Protein

Melibiose permease

Gene

melY

Organism
Enterobacter cloacae subsp. cloacae (strain ATCC 13047 / DSM 30054 / NBRC 13535 / NCTC 10005 / WDCM 00083 / NCDC 279-56)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Responsible for transport of melibiose into the cell, with the concomitant import of a proton (symport system) (PubMed:9209070, PubMed:9375783, PubMed:12775706, PubMed:18177889).

Can also transport lactose, and has weak activity with maltose (PubMed:18177889, PubMed:12775706).

Cannot transport the analog methyl-1-thio-beta,D-galactopyranoside (TMG) (PubMed:18177889).

4 Publications

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=0.26 mM for melibiose1 Publication
  2. KM=0.51 mM for lactose1 Publication
  3. KM=14 mM for maltose1 Publication
  1. Vmax=82 nmol/min/mg enzyme with melibiose as substrate1 Publication
  2. Vmax=54 nmol/min/mg enzyme with lactose as substrate1 Publication
  3. Vmax=121 nmol/min/mg enzyme with maltose as substrate1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processSugar transport, Transport

Protein family/group databases

Transport Classification Database

More...
TCDBi
2.A.1.5.4, the major facilitator superfamily (mfs)

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Melibiose permease1 Publication
Alternative name(s):
Melibiose transporter MelYCurated
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:melY2 Publications
Ordered Locus Names:ECL_05077Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEnterobacter cloacae subsp. cloacae (strain ATCC 13047 / DSM 30054 / NBRC 13535 / NCTC 10005 / WDCM 00083 / NCDC 279-56)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri716541 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002363 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 13Cytoplasmic1 PublicationAdd BLAST13
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei14 – 34HelicalSequence analysisAdd BLAST21
Topological domaini35 – 50Periplasmic1 PublicationAdd BLAST16
Transmembranei51 – 71HelicalSequence analysisAdd BLAST21
Topological domaini72 – 80Cytoplasmic1 Publication9
Transmembranei81 – 101HelicalSequence analysisAdd BLAST21
Topological domaini102 – 107Periplasmic1 Publication6
Transmembranei108 – 128HelicalSequence analysisAdd BLAST21
Topological domaini129 – 149Cytoplasmic1 PublicationAdd BLAST21
Transmembranei150 – 170HelicalSequence analysisAdd BLAST21
Topological domaini171Periplasmic1 Publication1
Transmembranei172 – 192HelicalSequence analysisAdd BLAST21
Topological domaini193 – 227Cytoplasmic1 PublicationAdd BLAST35
Transmembranei228 – 248HelicalSequence analysisAdd BLAST21
Topological domaini249 – 267Periplasmic1 PublicationAdd BLAST19
Transmembranei268 – 288HelicalSequence analysisAdd BLAST21
Topological domaini289 – 297Cytoplasmic1 Publication9
Transmembranei298 – 318HelicalSequence analysisAdd BLAST21
Topological domaini319 – 325Periplasmic1 Publication7
Transmembranei326 – 346HelicalSequence analysisAdd BLAST21
Topological domaini347 – 353Cytoplasmic1 Publication7
Transmembranei354 – 374HelicalSequence analysisAdd BLAST21
Topological domaini375 – 385Periplasmic1 PublicationAdd BLAST11
Transmembranei386 – 406HelicalSequence analysisAdd BLAST21
Topological domaini407 – 425Cytoplasmic1 PublicationAdd BLAST19

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi88L → P: Does not affect melibiose transport. Increases affinity for maltose and maltose transport. 1 Publication1
Mutagenesisi91L → P: Does not affect melibiose transport. Increases affinity for maltose and maltose transport. 1 Publication1
Mutagenesisi182A → P: Does not affect melibiose transport. Increases affinity for maltose and maltose transport. 1 Publication1
Mutagenesisi367A → V: No change in transport activity. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004427421 – 425Melibiose permeaseAdd BLAST425

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
716541.ECL_05077

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG2211, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_055585_0_0_6

Identification of Orthologs from Complete Genome Data

More...
OMAi
FIMATCF

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR000576, LacY/RafB_perm_fam
IPR018457, LacY/RafB_perm_fam_CS
IPR020846, MFS_dom
IPR036259, MFS_trans_sf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01306, LacY_symp, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR00174, LACYSMPORT

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF103473, SSF103473, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00882, 2A0105, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00896, LACY_1, 1 hit
PS00897, LACY_2, 1 hit
PS50850, MFS, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P96517-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNTTTCTHKD NPNFWIFGLF FFLYFFIMAT CFPFLPIWLS DIIGLNKTHT
60 70 80 90 100
GIVFSCISLS AIAFQPVLGV ISDKLGLKKH LLWIISVLLF LFAPFFLYVF
110 120 130 140 150
APLLKTNIWL GALSGGLYIG FVFSAGSGAI EAYIERVSRN SAFEYGKARM
160 170 180 190 200
FGCLGWGLCA STGGILFGID PSYVFWMGSA AALLLMLLLV VAKPKPNQTA
210 220 230 240 250
QVMNALGANQ PQITAKKVFN LFRQRRMWMF ILYVIGVACV YDVFDQQFAT
260 270 280 290 300
FFKTFFATPQ EGTRAFGFAT TAGEICNAII MFCSPWIINR IGAKNTLLIA
310 320 330 340 350
GLIMATRIIG SSFATTAVEV IALKMLHALE VPFLLVGAFK YITGVFDTRL
360 370 380 390 400
SATIYLIGFQ FAKQSAAIFL SAFAGNMYDR IGFQETYLML GCFVLAITVV
410 420
SAFTLSSRQE IAAAAGAAAL TSQSR
Length:425
Mass (Da):46,701
Last modified:May 1, 1997 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iD49AF033F9105D51
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AB000622 Genomic DNA Translation: BAA19154.1
CP001918 Genomic DNA Translation: ADF64599.1

NCBI Reference Sequences

More...
RefSeqi
WP_013099342.1, NC_014121.1
YP_003615548.1, NC_014121.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
ADF64599; ADF64599; ECL_05077

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
enc:ECL_05077

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|550.124.peg.580

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000622 Genomic DNA Translation: BAA19154.1
CP001918 Genomic DNA Translation: ADF64599.1
RefSeqiWP_013099342.1, NC_014121.1
YP_003615548.1, NC_014121.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

STRINGi716541.ECL_05077

Protein family/group databases

TCDBi2.A.1.5.4, the major facilitator superfamily (mfs)

Genome annotation databases

EnsemblBacteriaiADF64599; ADF64599; ECL_05077
KEGGienc:ECL_05077
PATRICifig|550.124.peg.580

Phylogenomic databases

eggNOGiCOG2211, Bacteria
HOGENOMiCLU_055585_0_0_6
OMAiFIMATCF

Family and domain databases

InterProiView protein in InterPro
IPR000576, LacY/RafB_perm_fam
IPR018457, LacY/RafB_perm_fam_CS
IPR020846, MFS_dom
IPR036259, MFS_trans_sf
PfamiView protein in Pfam
PF01306, LacY_symp, 1 hit
PRINTSiPR00174, LACYSMPORT
SUPFAMiSSF103473, SSF103473, 1 hit
TIGRFAMsiTIGR00882, 2A0105, 1 hit
PROSITEiView protein in PROSITE
PS00896, LACY_1, 1 hit
PS00897, LACY_2, 1 hit
PS50850, MFS, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiMELY_ENTCC
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P96517
Secondary accession number(s): A0A0H3CTM4
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2017
Last sequence update: May 1, 1997
Last modified: June 2, 2021
This is version 102 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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