UniProtKB - P96086 (TRI_THEAC)
Protein
Tricorn protease
Gene
tri
Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Functioni
Tricorn degrades oligopeptides (probably derived from the proteasome) and channels the products to F1, F2 and F3 proteases, which then catalyze the terminal degradation step, yielding free amino acids.
pH dependencei
Optimum pH is 8.5-8.8.
Temperature dependencei
Optimum temperature is 65 degrees Celsius.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 746 | Charge relay system2 Publications | 1 | |
Sitei | 936 | Substrate specificity switch1 Publication | 1 | |
Active sitei | 965 | Nucleophile2 Publications | 1 | |
Sitei | 966 | Transition state stabilizer; via amide nitrogen2 Publications | 1 | |
Active sitei | 1023 | Charge relay system1 Publication | 1 |
GO - Molecular functioni
- serine-type peptidase activity Source: UniProtKB
GO - Biological processi
- proteolysis Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Enzyme and pathway databases
BioCyci | TACI273075:G1GT7-1625-MONOMER |
BRENDAi | 3.4.21.B34, 6324 |
Protein family/group databases
MEROPSi | S41.005 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:tri Ordered Locus Names:Ta1490 |
Organismi | Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) |
Taxonomic identifieri | 273075 [NCBI] |
Taxonomic lineagei | Archaea › Euryarchaeota › Diaforarchaea group › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 131 – 132 | RR → EE: Decreased catalytic activity towards protein substrates. Retains 10% of wild-type activity towards casein and about 30% towards oxidized insulin beta chain. However, there is no change in the activity for a tripeptide substrate. 2 Publications | 2 | |
Mutagenesisi | 184 | L → C: Both peptidolytic and proteolytic activities doubled, probably due to the increase of the diameter of the channel for product exit. Retains less than 50% of wild-type activity after modification of the thiol group by maleimide, which decreases the diameter of the exit channel and impairs product exit from the catalytic chamber. 2 Publications | 1 | |
Mutagenesisi | 414 | R → C: Retains 50% of wild-type activity after modification of the thiol group by maleimide, which decreases the diameter of the access channel and impairs substrate access to the active site. Decreased catalytic activity towards fluorogenic substrate and insulin beta chain prior to any modification or oxidation, probably due to the decrease of the diameter of the substrate access channel. Further decreased catalytic activity towards these substrates after modification with fluorescein-5-maleimide or oxidation by oxidized glutathione; when associated with C-643. 2 Publications | 1 | |
Mutagenesisi | 643 | A → C: Decreased catalytic activity towards fluorogenic substrate and insulin beta chain prior to any modification or oxidation. Further decreased catalytic activity towards these substrates after modification with fluorescein-5-maleimide or oxidation by oxidized glutathione; when associated with C-414. 1 Publication | 1 | |
Mutagenesisi | 746 | H → A: Loss of catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 965 | S → A: Loss of catalytic activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000207197 | 1 – 1071 | Tricorn proteaseAdd BLAST | 1071 |
Interactioni
Subunit structurei
Part of the Tricorn proteolytic complex. Assembles to form a hexameric toroid, 20 copies of which may then assemble to form an icosahedral supermolecule of 14.6 MDa.
4 PublicationsProtein-protein interaction databases
STRINGi | 273075.Ta1490 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P96086 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P96086 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 39 – 310 | Six-bladed beta propeller1 PublicationAdd BLAST | 272 | |
Regioni | 131 – 132 | Binds the substrate's C-terminus2 Publications | 2 | |
Regioni | 326 – 675 | Seven-bladed beta propeller1 PublicationAdd BLAST | 350 | |
Regioni | 679 – 745 | C-1; helical bundle1 PublicationAdd BLAST | 67 | |
Regioni | 761 – 855 | PDZ-like1 PublicationAdd BLAST | 95 | |
Regioni | 856 – 1061 | C-2; alpha-beta sandwich1 PublicationAdd BLAST | 206 | |
Regioni | 916 – 918 | Substrate binding2 Publications | 3 | |
Regioni | 993 – 995 | Substrate binding1 Publication | 3 |
Domaini
It is thought that substrate reaches the active site through the seven-bladed beta propeller channel, while products exit via the six-bladed beta propeller channel.3 Publications
Sequence similaritiesi
Belongs to the peptidase S41B family.Curated
Phylogenomic databases
eggNOGi | arCOG03384, Archaea |
HOGENOMi | CLU_005503_1_0_2 |
OMAi | YLHVPDM |
OrthoDBi | 456at2157 |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR029045, ClpP/crotonase-like_dom_sf IPR011659, PD40 IPR036034, PDZ_sf IPR005151, Tail-specific_protease IPR028204, Tricorn_C1 IPR029414, Tricorn_PDZ IPR012393, Tricorn_protease IPR015943, WD40/YVTN_repeat-like_dom_sf |
PANTHERi | PTHR43253, PTHR43253, 1 hit |
Pfami | View protein in Pfam PF07676, PD40, 1 hit PF03572, Peptidase_S41, 1 hit PF14684, Tricorn_C1, 1 hit PF14685, Tricorn_PDZ, 1 hit |
PIRSFi | PIRSF036421, Tricorn_protease, 1 hit |
SMARTi | View protein in SMART SM00245, TSPc, 1 hit |
SUPFAMi | SSF50156, SSF50156, 1 hit SSF52096, SSF52096, 1 hit |
i Sequence
Sequence statusi: Complete.
P96086-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPSLMSFGSC QWIDQGRFSR SLYRNFKTFK LHEMHGLCMP NLLLNPDIHG
60 70 80 90 100
DRIIFVCCDD LWEHDLKSGS TRKIVSNLGV INNARFFPDG RKIAIRVMRG
110 120 130 140 150
SSLNTADLYF YNGENGEIKR ITYFSGKSTG RRMFTDVAGF DPDGNLIIST
160 170 180 190 200
DAMQPFSSMT CLYRVENDGI NFVPLNLGPA THILFADGRR VIGRNTFELP
210 220 230 240 250
HWKGYRGGTR GKIWIEVNSG AFKKIVDMST HVSSPVIVGH RIYFITDIDG
260 270 280 290 300
FGQIYSTDLD GKDLRKHTSF TDYYPRHLNT DGRRILFSKG GSIYIFNPDT
310 320 330 340 350
EKIEKIEIGD LESPEDRIIS IPSKFAEDFS PLDGDLIAFV SRGQAFIQDV
360 370 380 390 400
SGTYVLKVPE PLRIRYVRRG GDTKVAFIHG TREGDFLGIY DYRTGKAEKF
410 420 430 440 450
EENLGNVFAM GVDRNGKFAV VANDRFEIMT VDLETGKPTV IERSREAMIT
460 470 480 490 500
DFTISDNSRF IAYGFPLKHG ETDGYVMQAI HVYDMEGRKI FAATTENSHD
510 520 530 540 550
YAPAFDADSK NLYYLSYRSL DPSPDRVVLN FSFEVVSKPF VIPLIPGSPN
560 570 580 590 600
PTKLVPRSMT SEAGEYDLND MYKRSSPINV DPGDYRMIIP LESSILIYSV
610 620 630 640 650
PVHGEFAAYY QGAPEKGVLL KYDVKTRKVT EVKNNLTDLR LSADRKTVMV
660 670 680 690 700
RKDDGKIYTF PLEKPEDERT VETDKRPLVS SIHEEFLQMY DEAWKLARDN
710 720 730 740 750
YWNEAVAKEI SERIYEKYRN LVPLCKTRYD LSNVIVEMQG EYRTSHSYEM
760 770 780 790 800
GGTFTDKDPF RSGRIACDFK LDGDHYVVAK AYAGDYSNEG EKSPIFEYGI
810 820 830 840 850
DPTGYLIEDI DGETVGAGSN IYRVLSEKAG TSARIRLSGK GGDKRDLMID
860 870 880 890 900
ILDDDRFIRY RSWVEANRRY VHERSKGTIG YIHIPDMGMM GLNEFYRLFI
910 920 930 940 950
NESSYQGLIV DVRFNGGGFV SQLIIEKLMN KRIGYDNPRR GTLSPYPTNS
960 970 980 990 1000
VRGKIIAITN EYAGSDGDIF SFSFKKLGLG KLIGTRTWGG VVGITPKRRL
1010 1020 1030 1040 1050
IDGTVLTQPE FAFWFRDAGF GVENYGVDPD VEIEYAPHDY LSGKDPQIDY
1060 1070
AIDALIEELR NWNEELPQRP S
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U72850 Genomic DNA Translation: AAC44621.1 AL445067 Genomic DNA Translation: CAC12608.1 |
PIRi | T43255 |
RefSeqi | WP_010901890.1, NC_002578.1 |
Genome annotation databases
EnsemblBacteriai | CAC12608; CAC12608; CAC12608 |
GeneIDi | 1456939 |
KEGGi | tac:Ta1490 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U72850 Genomic DNA Translation: AAC44621.1 AL445067 Genomic DNA Translation: CAC12608.1 |
PIRi | T43255 |
RefSeqi | WP_010901890.1, NC_002578.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1K32 | X-ray | 2.00 | A/B/C/D/E/F | 27-1071 | [»] | |
1N6D | X-ray | 2.80 | A/B/C/D/E/F | 1-1071 | [»] | |
1N6E | X-ray | 2.60 | A/C/E/G/I/K | 1-1071 | [»] | |
1N6F | X-ray | 2.70 | A/B/C/D/E/F | 1-1071 | [»] | |
SMRi | P96086 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 273075.Ta1490 |
Protein family/group databases
MEROPSi | S41.005 |
Genome annotation databases
EnsemblBacteriai | CAC12608; CAC12608; CAC12608 |
GeneIDi | 1456939 |
KEGGi | tac:Ta1490 |
Phylogenomic databases
eggNOGi | arCOG03384, Archaea |
HOGENOMi | CLU_005503_1_0_2 |
OMAi | YLHVPDM |
OrthoDBi | 456at2157 |
Enzyme and pathway databases
BioCyci | TACI273075:G1GT7-1625-MONOMER |
BRENDAi | 3.4.21.B34, 6324 |
Miscellaneous databases
EvolutionaryTracei | P96086 |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR029045, ClpP/crotonase-like_dom_sf IPR011659, PD40 IPR036034, PDZ_sf IPR005151, Tail-specific_protease IPR028204, Tricorn_C1 IPR029414, Tricorn_PDZ IPR012393, Tricorn_protease IPR015943, WD40/YVTN_repeat-like_dom_sf |
PANTHERi | PTHR43253, PTHR43253, 1 hit |
Pfami | View protein in Pfam PF07676, PD40, 1 hit PF03572, Peptidase_S41, 1 hit PF14684, Tricorn_C1, 1 hit PF14685, Tricorn_PDZ, 1 hit |
PIRSFi | PIRSF036421, Tricorn_protease, 1 hit |
SMARTi | View protein in SMART SM00245, TSPc, 1 hit |
SUPFAMi | SSF50156, SSF50156, 1 hit SSF52096, SSF52096, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRI_THEAC | |
Accessioni | P96086Primary (citable) accession number: P96086 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | May 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 143 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries