UniProtKB - P96022 (DPO4_SACSO)
Protein
DNA polymerase IV
Gene
dbh
Organism
Saccharolobus solfataricus (Sulfolobus solfataricus)
Status
Functioni
Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis.
Catalytic activityi
- EC:2.7.7.7
Cofactori
Mg2+Note: Binds 2 magnesium ions per subunit.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 7 | Magnesium | 1 | |
Sitei | 12 | Substrate discrimination | 1 | |
Metal bindingi | 105 | Magnesium | 1 | |
Active sitei | 106 | 1 |
GO - Molecular functioni
- damaged DNA binding Source: InterPro
- DNA-directed DNA polymerase activity Source: UniProtKB-UniRule
- magnesium ion binding Source: UniProtKB-UniRule
GO - Biological processi
- DNA-dependent DNA replication Source: UniProtKB-UniRule
- DNA repair Source: UniProtKB-UniRule
Keywordsi
Molecular function | DNA-binding, DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase |
Biological process | DNA damage, DNA repair, DNA replication |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 2.7.7.7, 6163 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:dbh |
Organismi | Saccharolobus solfataricus (Sulfolobus solfataricus) |
Taxonomic identifieri | 2287 [NCBI] |
Taxonomic lineagei | Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Saccharolobus |
Subcellular locationi
- Cytoplasm Curated
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000173975 | 1 – 354 | DNA polymerase IVAdd BLAST | 354 |
Interactioni
Subunit structurei
Monomer.
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P96022 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P96022 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 188 | UmuCAdd BLAST | 186 |
Domaini
The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity. It lacks the O helices present in high-fidelity DNA polymerases in the fingers domain.
Sequence similaritiesi
Belongs to the DNA polymerase type-Y family.Curated
Family and domain databases
CDDi | cd03586, PolY_Pol_IV_kappa, 1 hit |
Gene3Di | 3.30.1490.100, 1 hit 3.30.70.270, 1 hit |
HAMAPi | MF_01113, DNApol_IV, 1 hit |
InterProi | View protein in InterPro IPR043502, DNA/RNA_pol_sf IPR036775, DNA_pol_Y-fam_lit_finger_sf IPR022880, DNApol_IV IPR024728, PolY_HhH_motif IPR043128, Rev_trsase/Diguanyl_cyclase IPR001126, UmuC |
Pfami | View protein in Pfam PF00817, IMS, 1 hit PF11798, IMS_HHH, 1 hit |
SUPFAMi | SSF100879, SSF100879, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS50173, UMUC, 1 hit |
i Sequence
Sequence statusi: Complete.
P96022-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIVIFVDFDY FFAQVEEVLN PQYKGKPLVV CVYSGRTKTS GAVATANYEA
60 70 80 90 100
RKLGVKAGMP IIKAMQIAPS AIYVPMRKPI YEAFSNRIMN LLNKHADKIE
110 120 130 140 150
VASIDEAYLD VTNKVEGNFE NGIELARKIK QEILEKEKIT VTVGVAPNKI
160 170 180 190 200
LAKIIADKSK PNGLGVIRPT EVQDFLNELD IDEIPGIGSV LARRLNELGI
210 220 230 240 250
QKLRDILSKN YNELEKITGK AKALYLLKLA QNKYSEPVEN KSKIPHGRYL
260 270 280 290 300
TLPYNTRDVK VILPYLKKAI NEAYNKVNGI PMRITVIAIM EDLDILSKGK
310 320 330 340 350
KFKHGISIDN AYKVAEDLLR ELLVRDKRRN VRRIGVKLDN IIINKTNLSD
FFDI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U52110 Genomic DNA Translation: AAB38090.1 |
PIRi | T46875 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U52110 Genomic DNA Translation: AAB38090.1 |
PIRi | T46875 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1IM4 | X-ray | 2.30 | A | 2-216 | [»] | |
1K1Q | X-ray | 2.80 | A/B | 1-354 | [»] | |
1K1S | X-ray | 2.80 | A | 1-354 | [»] | |
SMRi | P96022 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Enzyme and pathway databases
BRENDAi | 2.7.7.7, 6163 |
Miscellaneous databases
EvolutionaryTracei | P96022 |
Family and domain databases
CDDi | cd03586, PolY_Pol_IV_kappa, 1 hit |
Gene3Di | 3.30.1490.100, 1 hit 3.30.70.270, 1 hit |
HAMAPi | MF_01113, DNApol_IV, 1 hit |
InterProi | View protein in InterPro IPR043502, DNA/RNA_pol_sf IPR036775, DNA_pol_Y-fam_lit_finger_sf IPR022880, DNApol_IV IPR024728, PolY_HhH_motif IPR043128, Rev_trsase/Diguanyl_cyclase IPR001126, UmuC |
Pfami | View protein in Pfam PF00817, IMS, 1 hit PF11798, IMS_HHH, 1 hit |
SUPFAMi | SSF100879, SSF100879, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS50173, UMUC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DPO4_SACSO | |
Accessioni | P96022Primary (citable) accession number: P96022 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 2, 2002 |
Last sequence update: | May 1, 1997 | |
Last modified: | August 12, 2020 | |
This is version 103 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families