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Protein

Gingipain R2

Gene

rgpB

Organism
Porphyromonas gingivalis (strain ATCC BAA-308 / W83)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Thiol protease. Acts synergistically with RgpA to catalyze the maturation of fimbrial subunits, such as FimA (By similarity). Its proteolytic activity is a major factor in both periodontal tissue destruction and in evasion of host defense mechanisms (Probable).By similarityCurated

Catalytic activityi

Hydrolysis of proteins and small molecule substrates, with a preference for Arg in P1.2 Publications

Activity regulationi

Inhibited by human histatin-3 1/24 (histatin-5).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi307Calcium 1Combined sources1
Metal bindingi329Calcium 2; via carbonyl oxygenCombined sources1
Metal bindingi332Calcium 2Combined sources1
Metal bindingi334Calcium 2; via carbonyl oxygenCombined sources1
Metal bindingi336Calcium 2Combined sources1
Metal bindingi390Calcium 3Combined sources1
Metal bindingi395Calcium 3; via pros nitrogenCombined sources1
Active sitei440Proton donorCombined sources1 Publication1
Active sitei473NucleophileCombined sources1 Publication1 Publication1
Metal bindingi478Calcium 1; via carbonyl oxygenCombined sources1
Metal bindingi487Calcium 1Combined sources1
Metal bindingi521Calcium 3Combined sources1
Metal bindingi522Calcium 4Combined sources1
Metal bindingi525Calcium 4Combined sources1
Metal bindingi531Calcium 4; via tele nitrogenCombined sources1
Metal bindingi613Calcium 5Combined sources1
Metal bindingi639Calcium 5Combined sources1

GO - Molecular functioni

  • calcium-dependent cysteine-type endopeptidase activity Source: UniProtKB
  • calcium ion binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processVirulence
LigandCalcium, Metal-binding

Enzyme and pathway databases

BioCyciPGIN242619:G1G02-466-MONOMER
BRENDAi3.4.22.37 756
SABIO-RKiP95493

Protein family/group databases

MEROPSiC25.003

Names & Taxonomyi

Protein namesi
Recommended name:
Gingipain R2 (EC:3.4.22.372 Publications)
Alternative name(s):
Arg-gingipain
Gingipain 2
RGP-2
Gene namesi
Name:rgpB
Synonyms:prtRII, rgp2
Ordered Locus Names:PG_0506
OrganismiPorphyromonas gingivalis (strain ATCC BAA-308 / W83)
Taxonomic identifieri242619 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesPorphyromonadaceaePorphyromonas
Proteomesi
  • UP000000588 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000002653525 – 2291 PublicationAdd BLAST205
ChainiPRO_0000026536230 – 736Gingipain R2Add BLAST507

Keywords - PTMi

Zymogen

Proteomic databases

PRIDEiP95493

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
HTN3P155167EBI-8505881,EBI-738783From Homo sapiens.

Protein-protein interaction databases

IntActiP95493, 1 interactor
MINTiP95493
STRINGi242619.PG0506

Structurei

Secondary structure

1736
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP95493
SMRiP95493
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP95493

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C25 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108MCP Bacteria
ENOG4110H6N LUCA
KOiK08589
OMAiKENGRMI

Family and domain databases

CDDicd10913 Peptidase_C25_N_gingipain, 1 hit
Gene3Di2.60.40.10, 1 hit
2.60.40.3800, 1 hit
3.40.50.10390, 1 hit
InterProiView protein in InterPro
IPR029030 Caspase-like_dom_sf
IPR001769 Gingipain
IPR039392 Gingipain_N
IPR029031 Gingipain_N_sf
IPR038490 Gingipain_propep_sf
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
IPR005536 Peptidase_C25_Ig-like_domain
IPR012600 Propeptide_C25
IPR026444 Secre_tail
PfamiView protein in Pfam
PF01364 Peptidase_C25, 1 hit
PF03785 Peptidase_C25_C, 1 hit
PF08126 Propeptide_C25, 1 hit
SUPFAMiSSF52129 SSF52129, 1 hit
SSF81296 SSF81296, 1 hit
TIGRFAMsiTIGR04183 Por_Secre_tail, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P95493-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKKNFSRIVS IVAFSSLLGG MAFAQPAERG RNPQVRLLSA EQSMSKVQFR
60 70 80 90 100
MDNLQFTGVQ TSKGVAQVPT FTEGVNISEK GTPILPILSR SLAVSETRAM
110 120 130 140 150
KVEVVSSKFI EKKDVLIAPS KGVISRAENP DQIPYVYGQS YNEDKFFPGE
160 170 180 190 200
IATLSDPFIL RDVRGQVVNF APLQYNPVTK TLRIYTEIVV AVSETAEAGQ
210 220 230 240 250
NTISLVKNST FTGFEDIYKS VFMNYEATRY TPVEEKENGR MIVIVPKKYE
260 270 280 290 300
EDIEDFVDWK NQRGLRTEVK VAEDIASPVT ANAIQQFVKQ EYEKEGNDLT
310 320 330 340 350
YVLLVGDHKD IPAKITPGIK SDQVYGQIVG NDHYNEVFIG RFSCESKEDL
360 370 380 390 400
KTQIDRTIHY ERNITTEDKW LGQALCIASA EGGPSADNGE SDIQHENIIA
410 420 430 440 450
NLLTQYGYTK IIKCYDPGVT PKNIIDAFNG GISLANYTGH GSETAWGTSH
460 470 480 490 500
FGTTHVKQLT NSNQLPFIFD VACVNGDFLY NVPCFAEALM RAQKDGKPTG
510 520 530 540 550
TVAIIASTIN QSWASPMRGQ DEMNEILCEK HPNNIKRTFG GVTMNGMFAM
560 570 580 590 600
VEKYKKDGEK MLDTWTVFGD PSLLVRTLVP TKMQVTAPAN ISASAQTFEV
610 620 630 640 650
ACDYNGAIAT LSDDGDMVGT AIVKDGKAII KLNESIADET NLTLTVVGYN
660 670 680 690 700
KVTVIKDVKV EGTSIADVAN DKPYTVAVSG KTITVESPAA GLTIFDMNGR
710 720 730
RVATAKNRMV FEAQNGVYAV RIATEGKTYT EKVIVK
Length:736
Mass (Da):80,967
Last modified:October 3, 2003 - v2
Checksum:iC848DD3FAB420833
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti58G → D in AAB41892 (PubMed:9705298).Curated1
Sequence conflicti246P → A in AAB41892 (PubMed:9705298).Curated1
Sequence conflicti251E → G in AAB41892 (PubMed:9705298).Curated1
Sequence conflicti254E → K in AAB41892 (PubMed:9705298).Curated1
Sequence conflicti398I → V in AAB41892 (PubMed:9705298).Curated1
Sequence conflicti435A → V in AAB41892 (PubMed:9705298).Curated1
Sequence conflicti480 – 482YNV → FSM in AAB41892 (PubMed:9705298).Curated3
Sequence conflicti510N → D AA sequence (PubMed:9705298).Curated1
Sequence conflicti512S → Y AA sequence (PubMed:9705298).Curated1
Sequence conflicti515S → P AA sequence (PubMed:9705298).Curated1
Sequence conflicti560K → N in AAB41892 (PubMed:9705298).Curated1
Sequence conflicti582K → E in AAB41892 (PubMed:9705298).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U85038 Genomic DNA Translation: AAB41892.1
AF007124 Genomic DNA Translation: AAC26371.1
AE015924 Genomic DNA Translation: AAQ65700.1
RefSeqiWP_010956050.1, NC_002950.2

Genome annotation databases

EnsemblBacteriaiAAQ65700; AAQ65700; PG_0506
KEGGipgi:PG_0506
PATRICifig|242619.8.peg.464

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U85038 Genomic DNA Translation: AAB41892.1
AF007124 Genomic DNA Translation: AAC26371.1
AE015924 Genomic DNA Translation: AAQ65700.1
RefSeqiWP_010956050.1, NC_002950.2

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CVRX-ray2.00A230-664[»]
4IEFX-ray2.30A/C/E/G25-229[»]
B/D/F/H230-662[»]
5AG8X-ray1.90A/B577-736[»]
5AG9X-ray2.11A/B577-736[»]
5HFSX-ray1.97A/B665-736[»]
ProteinModelPortaliP95493
SMRiP95493
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP95493, 1 interactor
MINTiP95493
STRINGi242619.PG0506

Protein family/group databases

MEROPSiC25.003

Proteomic databases

PRIDEiP95493

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAQ65700; AAQ65700; PG_0506
KEGGipgi:PG_0506
PATRICifig|242619.8.peg.464

Phylogenomic databases

eggNOGiENOG4108MCP Bacteria
ENOG4110H6N LUCA
KOiK08589
OMAiKENGRMI

Enzyme and pathway databases

BioCyciPGIN242619:G1G02-466-MONOMER
BRENDAi3.4.22.37 756
SABIO-RKiP95493

Miscellaneous databases

EvolutionaryTraceiP95493

Family and domain databases

CDDicd10913 Peptidase_C25_N_gingipain, 1 hit
Gene3Di2.60.40.10, 1 hit
2.60.40.3800, 1 hit
3.40.50.10390, 1 hit
InterProiView protein in InterPro
IPR029030 Caspase-like_dom_sf
IPR001769 Gingipain
IPR039392 Gingipain_N
IPR029031 Gingipain_N_sf
IPR038490 Gingipain_propep_sf
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
IPR005536 Peptidase_C25_Ig-like_domain
IPR012600 Propeptide_C25
IPR026444 Secre_tail
PfamiView protein in Pfam
PF01364 Peptidase_C25, 1 hit
PF03785 Peptidase_C25_C, 1 hit
PF08126 Propeptide_C25, 1 hit
SUPFAMiSSF52129 SSF52129, 1 hit
SSF81296 SSF81296, 1 hit
TIGRFAMsiTIGR04183 Por_Secre_tail, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCPG2_PORGI
AccessioniPrimary (citable) accession number: P95493
Secondary accession number(s): O33441
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: October 3, 2003
Last modified: September 12, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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