UniProtKB - P94929 (LEU3_MYCBO)
Protein
3-isopropylmalate dehydrogenase
Gene
leuB
Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Functioni
Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate (By similarity).By similarity
Catalytic activityi
- EC:1.1.1.85
Cofactori
: L-leucine biosynthesis Pathwayi
This protein is involved in step 3 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.Proteins known to be involved in the 4 steps of the subpathway in this organism are:
- 2-isopropylmalate synthase (leuA), 2-isopropylmalate synthase (leuA)
- 3-isopropylmalate dehydratase small subunit (leuD), 3-isopropylmalate dehydratase small subunit (leuD), 3-isopropylmalate dehydratase large subunit (leuC), 3-isopropylmalate dehydratase large subunit (leuC)
- 3-isopropylmalate dehydrogenase (leuB), 3-isopropylmalate dehydrogenase (leuB)
- Branched-chain-amino-acid aminotransferase (ilvE)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 87 | SubstrateBy similarity | 1 | |
Binding sitei | 97 | SubstrateBy similarity | 1 | |
Binding sitei | 121 | SubstrateBy similarity | 1 | |
Sitei | 128 | Important for catalysisBy similarity | 1 | |
Sitei | 178 | Important for catalysisBy similarity | 1 | |
Metal bindingi | 211 | Magnesium or manganeseBy similarity | 1 | |
Binding sitei | 211 | SubstrateBy similarity | 1 | |
Metal bindingi | 235 | Magnesium or manganeseBy similarity | 1 | |
Metal bindingi | 239 | Magnesium or manganeseBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 271 – 283 | NADBy similarityAdd BLAST | 13 |
GO - Molecular functioni
- 3-isopropylmalate dehydrogenase activity Source: UniProtKB-UniRule
- magnesium ion binding Source: InterPro
- NAD binding Source: InterPro
GO - Biological processi
- leucine biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis |
Ligand | Magnesium, Manganese, Metal-binding, NAD |
Enzyme and pathway databases
BioCyci | MBOV233413:G1GT4-3172-MONOMER |
BRENDAi | 1.1.1.85, 3494 |
UniPathwayi | UPA00048;UER00072 |
Names & Taxonomyi
Protein namesi | Recommended name: 3-isopropylmalate dehydrogenase (EC:1.1.1.85)Alternative name(s): 3-IPM-DH Beta-IPM dehydrogenase Short name: IMDH |
Gene namesi | Name:leuB Ordered Locus Names:BQ2027_MB3019C |
Organismi | Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) |
Taxonomic identifieri | 233413 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm By similarity
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000083800 | 1 – 336 | 3-isopropylmalate dehydrogenaseAdd BLAST | 336 |
Interactioni
Subunit structurei
Homodimer.
By similarityFamily & Domainsi
Sequence similaritiesi
Phylogenomic databases
OMAi | EYDLGAR |
Family and domain databases
HAMAPi | MF_01035, LeuB_type2, 1 hit |
InterProi | View protein in InterPro IPR019818, IsoCit/isopropylmalate_DH_CS IPR024084, IsoPropMal-DH-like_dom IPR023698, LeuB_actb |
Pfami | View protein in Pfam PF00180, Iso_dh, 1 hit |
SMARTi | View protein in SMART SM01329, Iso_dh, 1 hit |
PROSITEi | View protein in PROSITE PS00470, IDH_IMDH, 1 hit |
i Sequence
Sequence statusi: Complete.
P94929-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKLAIIAGDG IGPEVTAEAV KVLDAVVPGV QKTSYDLGAR RFHATGEVLP
60 70 80 90 100
DSVVAELRNH DAILLGAIGD PSVPSGVLER GLLLRLRFEL DHHINLRPAR
110 120 130 140 150
LYPGVASPLS GNPGIDFVVV REGTEGPYTG NGGAIRVGTP NEVATEVSVN
160 170 180 190 200
TAFGVRRVVA DAFERARRRR KHLTLVHKTN VLTLAGGLWL RTVDEVGECY
210 220 230 240 250
PDVEVAYQHV DAATIHMITD PGRFDVIVTD NLFGDIITDL AAAVCGGIGL
260 270 280 290 300
AASGNIDATR ANPSMFEPVH GSAPDIAGQG IADPTAAIMS VALLLSHLGE
310 320 330
HDAAARVDRA VEAHLATRGS ERLATSDVGE RIAAAL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U78886 Genomic DNA Translation: AAC45173.1 LT708304 Genomic DNA Translation: SIU01643.1 |
RefSeqi | NP_856664.1, NC_002945.3 WP_003415144.1, NC_002945.4 |
Genome annotation databases
EnsemblBacteriai | CDO44287; CDO44287; Mb3019c |
PATRICi | fig|233413.5.peg.3318 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U78886 Genomic DNA Translation: AAC45173.1 LT708304 Genomic DNA Translation: SIU01643.1 |
RefSeqi | NP_856664.1, NC_002945.3 WP_003415144.1, NC_002945.4 |
3D structure databases
SMRi | P94929 |
ModBasei | Search... |
Genome annotation databases
EnsemblBacteriai | CDO44287; CDO44287; Mb3019c |
PATRICi | fig|233413.5.peg.3318 |
Phylogenomic databases
OMAi | EYDLGAR |
Enzyme and pathway databases
UniPathwayi | UPA00048;UER00072 |
BioCyci | MBOV233413:G1GT4-3172-MONOMER |
BRENDAi | 1.1.1.85, 3494 |
Family and domain databases
HAMAPi | MF_01035, LeuB_type2, 1 hit |
InterProi | View protein in InterPro IPR019818, IsoCit/isopropylmalate_DH_CS IPR024084, IsoPropMal-DH-like_dom IPR023698, LeuB_actb |
Pfami | View protein in Pfam PF00180, Iso_dh, 1 hit |
SMARTi | View protein in SMART SM01329, Iso_dh, 1 hit |
PROSITEi | View protein in PROSITE PS00470, IDH_IMDH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LEU3_MYCBO | |
Accessioni | P94929Primary (citable) accession number: P94929 Secondary accession number(s): A0A1R3Y2U0, X2BM37 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | May 1, 1997 | |
Last modified: | June 17, 2020 | |
This is version 128 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families