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UniProtKB - P94461 (PRIA_BACSU)

Entry version 138 (07 Apr 2021)
Sequence version 2 (15 Jul 1998)
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Protein

Primosomal protein N'

Gene

priA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA.

UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi295 – 302ATPUniRule annotation8
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section specifies the position(s) and type(s) of zinc fingers within the protein.<p><a href='/help/zn_fing' target='_top'>More...</a></p>Zinc fingeri510 – 522C4-typeUniRule annotationAdd BLAST13
Zinc fingeri537 – 553C4-typeUniRule annotationAdd BLAST17

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA replication
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		BSUB:BSU15710-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Primosomal protein N'UniRule annotation (EC:3.6.4.-UniRule annotation)
Alternative name(s):
ATP-dependent helicase PriAUniRule annotation
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:priAUniRule annotation
Synonyms:yloJ
Ordered Locus Names:BSU15710
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiBacillus subtilis (strain 168)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri224308 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000001570 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Primosome

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001021161 – 805Primosomal protein N'Add BLAST805

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P94461

PRoteomics IDEntifications database

More...PRIDEi		P94461

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Component of the primosome.

UniRule annotation

Protein-protein interaction databases

Protein interaction database and analysis system

More...IntActi		P94461, 1 interactor

STRING: functional protein association networks

More...STRINGi		224308.BSU15710

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini282 – 448Helicase ATP-bindingUniRule annotationAdd BLAST167
Domaini545 – 699Helicase C-terminalUniRule annotationAdd BLAST155

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi391 – 394DEAH box4

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the helicase family. PriA subfamily.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri510 – 522C4-typeUniRule annotationAdd BLAST13
Zinc fingeri537 – 553C4-typeUniRule annotationAdd BLAST17

Keywords - Domaini

Zinc-finger

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG1198, Bacteria

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P94461

Identification of Orthologs from Complete Genome Data

More...OMAi		RCHYCGY

Database for complete collections of gene phylogenies

More...PhylomeDBi		P94461

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.10.8.630, 1 hit

HAMAP database of protein families

More...HAMAPi		MF_00983, PriA, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR006935, Helicase/UvrB_N
IPR014001, Helicase_ATP-bd
IPR001650, Helicase_C
IPR027417, P-loop_NTPase
IPR005259, PriA
IPR041222, PriA_3primeBD
IPR042115, PriA_3primeBD_sf
IPR041236, PriA_C
IPR040498, PriA_CRR

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00271, Helicase_C, 1 hit
PF17764, PriA_3primeBD, 1 hit
PF18074, PriA_C, 1 hit
PF18319, PriA_CRR, 1 hit
PF04851, ResIII, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00487, DEXDc, 1 hit
SM00490, HELICc, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF52540, SSF52540, 2 hits

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR00595, priA, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS51192, HELICASE_ATP_BIND_1, 1 hit
PS51194, HELICASE_CTER, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P94461-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNFAEVIVDV STKNIDRPFD YKIPDHLKGM IKTGMRVIVP FGPRKIQGFV 
        60         70         80         90        100
TAVKEASDLS GKSVKEVEDL LDLTPVLTEE LMILSSWLSD KTLSFKITAL 
       110        120        130        140        150
QAMLPAALKA KYEKELKIAH GADLPPQVER LFSETKTLLY SDIPDHETLK 
       160        170        180        190        200
LIQRHVQKGD IDVTYKVAQK TNKKMVRHIQ ANASKEELAK QAEGLSRQAA 
       210        220        230        240        250
KQQAILHFLI SEPEGVKIPA AELCKKTDTS SATIKTLIQK GLLKESYEEV 
       260        270        280        290        300
YRDPYQDKMF KKTEPLPLTD EQRAAFEPIR ETLDSDEHKV FLLHGVTGSG 
       310        320        330        340        350
KTEIYLQSIE KVLAKGKEAI VLVPEISLTP QMVNRFKGRF GSQVAVMHSG 
       360        370        380        390        400
LSTGEKYDEW RKIHRKEVRL VVGARSAIFA PFENLGMIII DEEHESSYKQ 
       410        420        430        440        450
EEMPRYHAKE VAIKRAEHHS CPVVLGSATP TLESYARAQK GVYELLSLKH 
       460        470        480        490        500
RVNHRVMPEV SLVDMREELR NGNRSMFSVE LMEKLEETIA KGEQAVLFLN 
       510        520        530        540        550
KRGYSSFVMC RDCGYVPQCP HCDISMTYHR YGQRLKCHYC GHEEPVPHTC 
       560        570        580        590        600
PECASEHIRF FGTGTQRVEE ELTKVLPSAR VIRMDVDTTS RKGAHEKLLS 
       610        620        630        640        650
AFGEGKADIL LGTQMIAKGL DFPNVTLVGV LSADTTLHIP DFRSAEKTFQ 
       660        670        680        690        700
LLTQVSGRAG RHEKPGHVII QTYTPSHYSI QLTKTHDYET FYQHEMAHRR 
       710        720        730        740        750
EQSYPPYYYL ALVTVSHEEV AKAAVTAEKI AHFLKANCGA DTKILGPSAS 
       760        770        780        790        800
PIARIKDRYR YQCVIKYKQE TQLSALLKKI LEHYKREIEQ KHVMISIDMN 

PYMMM
Length:805
Mass (Da):91,353
Last modified:July 15, 1998 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i7E0D2970805AC948
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti232A → V in CAA71348 (PubMed:9086272).Curated1
Sequence conflicti691 – 698FYQHEMAH → VLSSMKWRT in CAA71348 (PubMed:9086272).Curated8

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
Y13937 Genomic DNA Translation: CAA74261.1
AL009126 Genomic DNA Translation: CAB13444.1
Y10304 Genomic DNA Translation: CAA71348.1

Protein sequence database of the Protein Information Resource

More...PIRi		A69682

NCBI Reference Sequences

More...RefSeqi		NP_389453.1, NC_000964.3
WP_003232079.1, NZ_JNCM01000035.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		CAB13444; CAB13444; BSU_15710

Database of genes from NCBI RefSeq genomes

More...GeneIDi		938122

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		bsu:BSU15710

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|224308.179.peg.1711

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13937 Genomic DNA Translation: CAA74261.1
AL009126 Genomic DNA Translation: CAB13444.1
Y10304 Genomic DNA Translation: CAA71348.1
PIRiA69682
RefSeqiNP_389453.1, NC_000964.3
WP_003232079.1, NZ_JNCM01000035.1

3D structure databases

Database of comparative protein structure models

More...ModBasei		Search...

SWISS-MODEL Interactive Workspace

More...SWISS-MODEL-Workspacei		Submit a new modelling project...

Protein-protein interaction databases

IntActiP94461, 1 interactor
STRINGi224308.BSU15710

Proteomic databases

PaxDbiP94461
PRIDEiP94461

Genome annotation databases

EnsemblBacteriaiCAB13444; CAB13444; BSU_15710
GeneIDi938122
KEGGibsu:BSU15710
PATRICifig|224308.179.peg.1711

Phylogenomic databases

eggNOGiCOG1198, Bacteria
InParanoidiP94461
OMAiRCHYCGY
PhylomeDBiP94461

Enzyme and pathway databases

BioCyciBSUB:BSU15710-MONOMER

Family and domain databases

Gene3Di1.10.8.630, 1 hit
HAMAPiMF_00983, PriA, 1 hit
InterProiView protein in InterPro
IPR006935, Helicase/UvrB_N
IPR014001, Helicase_ATP-bd
IPR001650, Helicase_C
IPR027417, P-loop_NTPase
IPR005259, PriA
IPR041222, PriA_3primeBD
IPR042115, PriA_3primeBD_sf
IPR041236, PriA_C
IPR040498, PriA_CRR
PfamiView protein in Pfam
PF00271, Helicase_C, 1 hit
PF17764, PriA_3primeBD, 1 hit
PF18074, PriA_C, 1 hit
PF18319, PriA_CRR, 1 hit
PF04851, ResIII, 1 hit
SMARTiView protein in SMART
SM00487, DEXDc, 1 hit
SM00490, HELICc, 1 hit
SUPFAMiSSF52540, SSF52540, 2 hits
TIGRFAMsiTIGR00595, priA, 1 hit
PROSITEiView protein in PROSITE
PS51192, HELICASE_ATP_BIND_1, 1 hit
PS51194, HELICASE_CTER, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPRIA_BACSU
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P94461
Secondary accession number(s): O34941
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 15, 1998
Last modified: April 7, 2021
This is version 138 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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