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UniProtKB - P94461 (PRIA_BACSU)
Protein
Primosomal protein N'
Gene
priA
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA.
UniRule annotationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 295 – 302 | ATPUniRule annotation | 8 | |
Zinc fingeri | 510 – 522 | C4-typeUniRule annotationAdd BLAST | 13 | |
Zinc fingeri | 537 – 553 | C4-typeUniRule annotationAdd BLAST | 17 |
GO - Molecular functioni
- 3'-5' DNA helicase activity Source: GO_Central
- ATP binding Source: UniProtKB-UniRule
- DNA binding Source: UniProtKB-UniRule
- hydrolase activity Source: UniProtKB-KW
- zinc ion binding Source: UniProtKB-UniRule
GO - Biological processi
- DNA recombination Source: GO_Central
- DNA replication, synthesis of RNA primer Source: UniProtKB-KW
- DNA replication initiation Source: GO_Central
- DNA unwinding involved in DNA replication Source: GO_Central
- double-strand break repair Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Helicase, Hydrolase |
Biological process | DNA replication |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
BioCyci | BSUB:BSU15710-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Primosomal protein N'UniRule annotation (EC:3.6.4.-UniRule annotation)Alternative name(s): ATP-dependent helicase PriAUniRule annotation |
Gene namesi | Name:priAUniRule annotation Synonyms:yloJ Ordered Locus Names:BSU15710 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Other locations
- primosome complex Source: UniProtKB-UniRule
Keywords - Cellular componenti
PrimosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000102116 | 1 – 805 | Primosomal protein N'Add BLAST | 805 |
Proteomic databases
PaxDbi | P94461 |
Interactioni
Subunit structurei
Component of the primosome.
UniRule annotationProtein-protein interaction databases
IntActi | P94461, 1 interactor |
STRINGi | 224308.BSU15710 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 282 – 448 | Helicase ATP-bindingUniRule annotationAdd BLAST | 167 | |
Domaini | 545 – 699 | Helicase C-terminalUniRule annotationAdd BLAST | 155 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 391 – 394 | DEAH box | 4 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 510 – 522 | C4-typeUniRule annotationAdd BLAST | 13 | |
Zinc fingeri | 537 – 553 | C4-typeUniRule annotationAdd BLAST | 17 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | COG1198, Bacteria |
InParanoidi | P94461 |
OMAi | RCHYCGY |
PhylomeDBi | P94461 |
Family and domain databases
Gene3Di | 3.40.1440.60, 1 hit 3.40.50.300, 2 hits |
HAMAPi | MF_00983, PriA, 1 hit |
InterProi | View protein in InterPro IPR006935, Helicase/UvrB_N IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR005259, PriA IPR041222, PriA_3primeBD IPR042115, PriA_3primeBD_sf IPR041236, PriA_C IPR040498, PriA_CRR |
Pfami | View protein in Pfam PF00271, Helicase_C, 1 hit PF17764, PriA_3primeBD, 1 hit PF18074, PriA_C, 1 hit PF18319, PriA_CRR, 1 hit PF04851, ResIII, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits |
TIGRFAMsi | TIGR00595, priA, 1 hit |
PROSITEi | View protein in PROSITE PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
i Sequence
Sequence statusi: Complete.
P94461-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNFAEVIVDV STKNIDRPFD YKIPDHLKGM IKTGMRVIVP FGPRKIQGFV
60 70 80 90 100
TAVKEASDLS GKSVKEVEDL LDLTPVLTEE LMILSSWLSD KTLSFKITAL
110 120 130 140 150
QAMLPAALKA KYEKELKIAH GADLPPQVER LFSETKTLLY SDIPDHETLK
160 170 180 190 200
LIQRHVQKGD IDVTYKVAQK TNKKMVRHIQ ANASKEELAK QAEGLSRQAA
210 220 230 240 250
KQQAILHFLI SEPEGVKIPA AELCKKTDTS SATIKTLIQK GLLKESYEEV
260 270 280 290 300
YRDPYQDKMF KKTEPLPLTD EQRAAFEPIR ETLDSDEHKV FLLHGVTGSG
310 320 330 340 350
KTEIYLQSIE KVLAKGKEAI VLVPEISLTP QMVNRFKGRF GSQVAVMHSG
360 370 380 390 400
LSTGEKYDEW RKIHRKEVRL VVGARSAIFA PFENLGMIII DEEHESSYKQ
410 420 430 440 450
EEMPRYHAKE VAIKRAEHHS CPVVLGSATP TLESYARAQK GVYELLSLKH
460 470 480 490 500
RVNHRVMPEV SLVDMREELR NGNRSMFSVE LMEKLEETIA KGEQAVLFLN
510 520 530 540 550
KRGYSSFVMC RDCGYVPQCP HCDISMTYHR YGQRLKCHYC GHEEPVPHTC
560 570 580 590 600
PECASEHIRF FGTGTQRVEE ELTKVLPSAR VIRMDVDTTS RKGAHEKLLS
610 620 630 640 650
AFGEGKADIL LGTQMIAKGL DFPNVTLVGV LSADTTLHIP DFRSAEKTFQ
660 670 680 690 700
LLTQVSGRAG RHEKPGHVII QTYTPSHYSI QLTKTHDYET FYQHEMAHRR
710 720 730 740 750
EQSYPPYYYL ALVTVSHEEV AKAAVTAEKI AHFLKANCGA DTKILGPSAS
760 770 780 790 800
PIARIKDRYR YQCVIKYKQE TQLSALLKKI LEHYKREIEQ KHVMISIDMN
PYMMM
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 232 | A → V in CAA71348 (PubMed:9086272).Curated | 1 | |
Sequence conflicti | 691 – 698 | FYQHEMAH → VLSSMKWRT in CAA71348 (PubMed:9086272).Curated | 8 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y13937 Genomic DNA Translation: CAA74261.1 AL009126 Genomic DNA Translation: CAB13444.1 Y10304 Genomic DNA Translation: CAA71348.1 |
PIRi | A69682 |
RefSeqi | NP_389453.1, NC_000964.3 WP_003232079.1, NZ_JNCM01000035.1 |
Genome annotation databases
EnsemblBacteriai | CAB13444; CAB13444; BSU_15710 |
GeneIDi | 938122 |
KEGGi | bsu:BSU15710 |
PATRICi | fig|224308.179.peg.1711 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y13937 Genomic DNA Translation: CAA74261.1 AL009126 Genomic DNA Translation: CAB13444.1 Y10304 Genomic DNA Translation: CAA71348.1 |
PIRi | A69682 |
RefSeqi | NP_389453.1, NC_000964.3 WP_003232079.1, NZ_JNCM01000035.1 |
3D structure databases
SMRi | P94461 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P94461, 1 interactor |
STRINGi | 224308.BSU15710 |
Proteomic databases
PaxDbi | P94461 |
Genome annotation databases
EnsemblBacteriai | CAB13444; CAB13444; BSU_15710 |
GeneIDi | 938122 |
KEGGi | bsu:BSU15710 |
PATRICi | fig|224308.179.peg.1711 |
Phylogenomic databases
eggNOGi | COG1198, Bacteria |
InParanoidi | P94461 |
OMAi | RCHYCGY |
PhylomeDBi | P94461 |
Enzyme and pathway databases
BioCyci | BSUB:BSU15710-MONOMER |
Family and domain databases
Gene3Di | 3.40.1440.60, 1 hit 3.40.50.300, 2 hits |
HAMAPi | MF_00983, PriA, 1 hit |
InterProi | View protein in InterPro IPR006935, Helicase/UvrB_N IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR005259, PriA IPR041222, PriA_3primeBD IPR042115, PriA_3primeBD_sf IPR041236, PriA_C IPR040498, PriA_CRR |
Pfami | View protein in Pfam PF00271, Helicase_C, 1 hit PF17764, PriA_3primeBD, 1 hit PF18074, PriA_C, 1 hit PF18319, PriA_CRR, 1 hit PF04851, ResIII, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits |
TIGRFAMsi | TIGR00595, priA, 1 hit |
PROSITEi | View protein in PROSITE PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PRIA_BACSU | |
Accessioni | P94461Primary (citable) accession number: P94461 Secondary accession number(s): O34941 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | July 15, 1998 | |
Last modified: | February 23, 2022 | |
This is version 140 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families