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Entry version 115 (13 Nov 2019)
Sequence version 1 (01 May 1997)
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Protein

FMN reductase [NAD(P)H]

Gene

nfrA2

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Reduces FMNH2 to FMN, with NADH or NADPH as reductant. It also reduces nitroaromatic compounds, quinones, chromates and azo dyes. It could supply the reduced form of FMN to luciferase-like protein and contribute to the degradation of aromatic compounds.1 Publication

Miscellaneous

Catalysis proceeds by a classical ping-pong bi-bi reaction mechanism. The reduction of nitro-organic compounds and inorganic chromate occur in the absence of external FMN and are therefore believed to require binding of these substrates in the active site. In contrast to these reduction processes, azo dyes are reduced only in the presence of external FMN, indicating that azo dye reduction occurs outside the active site of the enzyme.

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

FMN is a competitive inhibitor of NADH, and therefore leads to the preferential utilization of NADPH.1 Publication

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=0.2 µM for 4-nitrophenol (NP)(at pH 8 and 25 degrees Celisus)1 Publication
  2. KM=0.7 µM for 5-nitro-2-furaldehyde semicarbazone (NF)(at pH 8 and 25 degrees Celisus)1 Publication
  3. KM=4.2 µM for FMN (at pH 8 and 25 degrees Celisus)1 Publication
  4. KM=4.4 µM for NADPH (at pH 8 and 25 degrees Celisus)1 Publication
  5. KM=5.0 µM for chromate (at pH 8 and 25 degrees Celisus)1 Publication
  6. KM=6.4 µM for NADH (at pH 8 and 25 degrees Celisus)1 Publication
  7. KM=96 µM for methyl-4-nitrobenzenesulfonate (NBS)(at pH 8 and 25 degrees Celisus)1 Publication
  1. Vmax=0.9 µmol/min/mg enzyme with chromate as substrate (at pH 8 and 25 degrees Celisus)1 Publication
  2. Vmax=4.0 µmol/min/mg enzyme with 5-nitro-2-furaldehyde semicarbazone (nitrofurazone, NF) as substrate (at pH 8 and 25 degrees Celisus)1 Publication
  3. Vmax=7.0 µmol/min/mg enzyme with 4-nitrophenol (NP) as substrate (at pH 8 and 25 degrees Celisus)1 Publication
  4. Vmax=73.6 µmol/min/mg enzyme with methyl-4-nitrobenzenesulfonate (NBS) as substrate (at pH 8 and 25 degrees Celisus)1 Publication
  5. Vmax=200 µmol/min/mg enzyme with FMN as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei67FMN1 Publication1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionOxidoreductase
Biological processAromatic hydrocarbons catabolism, Detoxification
LigandFlavoprotein, FMN, NAD

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
BSUB:BSU03860-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
FMN reductase [NAD(P)H] (EC:1.5.1.391 Publication)
Alternative name(s):
NAD(P)H-dependent FMN reductase
NAD(P)H-dependent nitro/flavin reductase
NAD(P)H-dependent nitroreductase
NAD(P)H-dependent oxidoreductase
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:nfrA2
Synonyms:ycnD
Ordered Locus Names:BSU03860
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiBacillus subtilis (strain 168)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri224308 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000001570 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002055131 – 249FMN reductase [NAD(P)H]Add BLAST249

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P94424

PRoteomics IDEntifications database

More...
PRIDEi
P94424

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Strongly induced by stress due to exposure to 6-brom-2-vinyl-chroman-4-on (chromanon) and less strongly induced after exposure to 2-methylhydroquinone (2-MHQ) or catechol stress.1 Publication

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer.

1 Publication

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
224308.BSU03860

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1249
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P94424

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
P94424

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni11 – 15FMN-binding5
Regioni134 – 136FMN-binding3
Regioni173 – 175FMN-binding3

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the flavin oxidoreductase frp family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4108DBC Bacteria
COG0778 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000272869

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P94424

KEGG Orthology (KO)

More...
KOi
K19286

Identification of Orthologs from Complete Genome Data

More...
OMAi
FRHDETY

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P94424

Family and domain databases

Conserved Domains Database

More...
CDDi
cd02146 NfsA_FRP, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.40.109.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR016446 Flavin_OxRdtase_Frp
IPR029479 Nitroreductase
IPR000415 Nitroreductase-like

The PANTHER Classification System

More...
PANTHERi
PTHR43425 PTHR43425, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00881 Nitroreductase, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF005426 Frp, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF55469 SSF55469, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P94424-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNEVIKSLTD HRSIRSYTDE PVAQEQLDQI IEAVQSAPSS INGQQVTVIT
60 70 80 90 100
VQDKERKKKI SELAGGQPWI DQAPVFLLFC ADFNRAKIAL EDLHDFKMEI
110 120 130 140 150
TNGLESVLVG AVDAGIALGT ATAAAESLGL GTVPIGAVRG NPQELIELLE
160 170 180 190 200
LPKYVFPLSG LVIGHPADRS AKKPRLPQEA VNHQETYLNQ DELTSHIQAY
210 220 230 240
DEQMSEYMNK RTNGKETRNW SQSIASYYER LYYPHIREML EKQGFKVEK
Length:249
Mass (Da):27,868
Last modified:May 1, 1997 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i691BEAE44234FA59
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
D50453 Genomic DNA Translation: BAA09018.1
AF417208 Genomic DNA Translation: AAL09698.1
AL009126 Genomic DNA Translation: CAB12194.1

Protein sequence database of the Protein Information Resource

More...
PIRi
H69763

NCBI Reference Sequences

More...
RefSeqi
NP_388268.1, NC_000964.3
WP_003234479.1, NZ_JNCM01000031.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
CAB12194; CAB12194; BSU03860

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
938267

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
bsu:BSU03860

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|224308.179.peg.409

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50453 Genomic DNA Translation: BAA09018.1
AF417208 Genomic DNA Translation: AAL09698.1
AL009126 Genomic DNA Translation: CAB12194.1
PIRiH69763
RefSeqiNP_388268.1, NC_000964.3
WP_003234479.1, NZ_JNCM01000031.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZCHX-ray1.85A1-249[»]
SMRiP94424
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

STRINGi224308.BSU03860

Proteomic databases

PaxDbiP94424
PRIDEiP94424

Genome annotation databases

EnsemblBacteriaiCAB12194; CAB12194; BSU03860
GeneIDi938267
KEGGibsu:BSU03860
PATRICifig|224308.179.peg.409

Phylogenomic databases

eggNOGiENOG4108DBC Bacteria
COG0778 LUCA
HOGENOMiHOG000272869
InParanoidiP94424
KOiK19286
OMAiFRHDETY
PhylomeDBiP94424

Enzyme and pathway databases

BioCyciBSUB:BSU03860-MONOMER

Miscellaneous databases

EvolutionaryTraceiP94424

Family and domain databases

CDDicd02146 NfsA_FRP, 1 hit
Gene3Di3.40.109.10, 1 hit
InterProiView protein in InterPro
IPR016446 Flavin_OxRdtase_Frp
IPR029479 Nitroreductase
IPR000415 Nitroreductase-like
PANTHERiPTHR43425 PTHR43425, 1 hit
PfamiView protein in Pfam
PF00881 Nitroreductase, 1 hit
PIRSFiPIRSF005426 Frp, 1 hit
SUPFAMiSSF55469 SSF55469, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNFRA2_BACSU
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P94424
Secondary accession number(s): Q548A6
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 1, 1997
Last modified: November 13, 2019
This is version 115 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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