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UniProtKB - P94368 (NNRD_BACSU)
Protein
ADP-dependent (S)-NAD(P)H-hydrate dehydratase
Gene
nnrD
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.
UniRule annotation1 PublicationCatalytic activityi
- EC:4.2.1.136UniRule annotation1 PublicationThis reaction proceeds in the forward1 Publication direction.
- EC:4.2.1.136UniRule annotation1 PublicationThis reaction proceeds in the forward1 Publication direction.
Cofactori
Mg2+UniRule annotation1 Publication
Kineticsi
kcat is 0.35 sec(-1) for the ADP-dependent dehydration of NADHX.1 Publication
- KM=0.30 µM for NADHX1 Publication
- KM=6.43 µM for ADP1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 42 | NADPHX; via amide nitrogenCombined sources1 Publication | 1 | |
Binding sitei | 104 | NADPHX; via amide nitrogenCombined sources1 Publication | 1 | |
Binding sitei | 149 | NADPHXCombined sources1 Publication | 1 | |
Binding sitei | 215 | AMP; via amide nitrogenCombined sources1 Publication | 1 | |
Binding sitei | 216 | NADPHXCombined sources1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 186 – 190 | AMPCombined sources1 Publication | 5 |
GO - Molecular functioni
- ADP-dependent NAD(P)H-hydrate dehydratase activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- NADHX epimerase activity Source: GO_Central
- NADPHX epimerase activity Source: GO_Central
GO - Biological processi
- metabolite repair Source: GO_Central
- nicotinamide nucleotide metabolic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Lyase |
Ligand | ATP-binding, NAD, NADP, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU38720-MONOMER |
BRENDAi | 4.2.1.136, 658 4.2.1.93, 658 |
Names & Taxonomyi
Protein namesi | Recommended name: ADP-dependent (S)-NAD(P)H-hydrate dehydrataseUniRule annotation (EC:4.2.1.136UniRule annotation)Alternative name(s): ADP-dependent NAD(P)HX dehydrataseUniRule annotation |
Gene namesi | Name:nnrDUniRule annotation Synonyms:yxkO Ordered Locus Names:BSU38720 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000119046 | 1 – 276 | ADP-dependent (S)-NAD(P)H-hydrate dehydrataseAdd BLAST | 276 |
Proteomic databases
PaxDbi | P94368 |
Interactioni
Subunit structurei
Homotetramer.
UniRule annotation2 PublicationsProtein-protein interaction databases
DIPi | DIP-59951N |
STRINGi | 224308.BSU38720 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P94368 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P94368 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 7 – 275 | YjeF C-terminalUniRule annotationAdd BLAST | 269 |
Sequence similaritiesi
Belongs to the NnrD/CARKD family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0063, Bacteria |
InParanoidi | P94368 |
OMAi | ATYWRDG |
PhylomeDBi | P94368 |
Family and domain databases
CDDi | cd01171, YXKO-related, 1 hit |
Gene3Di | 3.40.1190.20, 1 hit |
HAMAPi | MF_01965, NADHX_dehydratase, 1 hit |
InterProi | View protein in InterPro IPR017953, Carbohydrate_kinase_pred_CS IPR000631, CARKD IPR029056, Ribokinase-like |
Pfami | View protein in Pfam PF01256, Carb_kinase, 1 hit |
SUPFAMi | SSF53613, SSF53613, 1 hit |
TIGRFAMsi | TIGR00196, yjeF_cterm, 1 hit |
PROSITEi | View protein in PROSITE PS01049, YJEF_C_1, 1 hit PS01050, YJEF_C_2, 1 hit PS51383, YJEF_C_3, 1 hit |
i Sequence
Sequence statusi: Complete.
P94368-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNVPFWTEEH VRATLPERDA ESHKGTYGTA LLLAGSDDMP GAALLAGLGA
60 70 80 90 100
MRSGLGKLVI GTSENVIPLI VPVLPEATYW RDGWKKAADA QLEETYRAIA
110 120 130 140 150
IGPGLPQTES VQQAVDHVLT ADCPVILDAG ALAKRTYPKR EGPVILTPHP
160 170 180 190 200
GEFFRMTGVP VNELQKKRAE YAKEWAAQLQ TVIVLKGNQT VIAFPDGDCW
210 220 230 240 250
LNPTGNGALA KGGTGDTLTG MILGMLCCHE DPKHAVLNAV YLHGACAELW
260 270
TDEHSAHTLL AHELSDILPR VWKRFE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D83026 Genomic DNA Translation: BAA11731.1 AL009126 Genomic DNA Translation: CAB15898.1 |
PIRi | D70081 |
RefSeqi | NP_391751.1, NC_000964.3 WP_003244033.1, NZ_JNCM01000034.1 |
Genome annotation databases
EnsemblBacteriai | CAB15898; CAB15898; BSU_38720 |
GeneIDi | 937408 |
KEGGi | bsu:BSU38720 |
PATRICi | fig|224308.179.peg.4191 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D83026 Genomic DNA Translation: BAA11731.1 AL009126 Genomic DNA Translation: CAB15898.1 |
PIRi | D70081 |
RefSeqi | NP_391751.1, NC_000964.3 WP_003244033.1, NZ_JNCM01000034.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1KYH | X-ray | 1.60 | A | 1-276 | [»] | |
3RPH | X-ray | 1.75 | A | 1-276 | [»] | |
3RPZ | X-ray | 1.51 | A | 1-276 | [»] | |
3RQ2 | X-ray | 1.80 | A | 1-276 | [»] | |
3RQ5 | X-ray | 1.70 | A | 1-276 | [»] | |
3RQ6 | X-ray | 1.65 | A | 1-276 | [»] | |
3RQ8 | X-ray | 1.90 | A | 1-276 | [»] | |
3RQH | X-ray | 1.75 | A | 1-276 | [»] | |
3RQQ | X-ray | 1.60 | A | 1-276 | [»] | |
3RQX | X-ray | 1.60 | A | 1-276 | [»] | |
SMRi | P94368 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-59951N |
STRINGi | 224308.BSU38720 |
Proteomic databases
PaxDbi | P94368 |
Protocols and materials databases
DNASUi | 937408 |
Genome annotation databases
EnsemblBacteriai | CAB15898; CAB15898; BSU_38720 |
GeneIDi | 937408 |
KEGGi | bsu:BSU38720 |
PATRICi | fig|224308.179.peg.4191 |
Phylogenomic databases
eggNOGi | COG0063, Bacteria |
InParanoidi | P94368 |
OMAi | ATYWRDG |
PhylomeDBi | P94368 |
Enzyme and pathway databases
BioCyci | BSUB:BSU38720-MONOMER |
BRENDAi | 4.2.1.136, 658 4.2.1.93, 658 |
Miscellaneous databases
EvolutionaryTracei | P94368 |
Family and domain databases
CDDi | cd01171, YXKO-related, 1 hit |
Gene3Di | 3.40.1190.20, 1 hit |
HAMAPi | MF_01965, NADHX_dehydratase, 1 hit |
InterProi | View protein in InterPro IPR017953, Carbohydrate_kinase_pred_CS IPR000631, CARKD IPR029056, Ribokinase-like |
Pfami | View protein in Pfam PF01256, Carb_kinase, 1 hit |
SUPFAMi | SSF53613, SSF53613, 1 hit |
TIGRFAMsi | TIGR00196, yjeF_cterm, 1 hit |
PROSITEi | View protein in PROSITE PS01049, YJEF_C_1, 1 hit PS01050, YJEF_C_2, 1 hit PS51383, YJEF_C_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NNRD_BACSU | |
Accessioni | P94368Primary (citable) accession number: P94368 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | May 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 139 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families