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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

nodM

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the production of the root hair deformation (HAD) factor specifically on soybean.By similarity

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2Nucleophile; for GATase activityBy similarity1
Active sitei603For Fru-6P isomerization activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Transferase
Biological processNodulation

Enzyme and pathway databases

BioCyciBDIA224911:G1G3J-1651-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
Short name:
GFAT
Alternative name(s):
Nodulation protein M
Gene namesi
Name:nodM
Ordered Locus Names:blr1632
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000002526 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: GO_Central

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001354342 – 608Glutamine--fructose-6-phosphate aminotransferase [isomerizing]Add BLAST607

Proteomic databases

PRIDEiP94323

Interactioni

Protein-protein interaction databases

STRINGi224911.blr1632

Structurei

3D structure databases

ProteinModelPortaliP94323
SMRiP94323
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 217Glutamine amidotransferase type-2Add BLAST216
Domaini284 – 423SIS 1Add BLAST140
Domaini456 – 598SIS 2Add BLAST143

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiENOG4105C46 Bacteria
COG0449 LUCA
HOGENOMiHOG000258896
InParanoidiP94323
KOiK00820
OMAiTSYYSGC
OrthoDBiPOG091H0061
PhylomeDBiP94323

Family and domain databases

CDDicd05008 SIS_GlmS_GlmD_1, 1 hit
cd05009 SIS_GlmS_GlmD_2, 1 hit
Gene3Di3.60.20.10, 1 hit
HAMAPiMF_00164 GlmS, 1 hit
InterProiView protein in InterPro
IPR017932 GATase_2_dom
IPR035466 GlmS/AgaS_SIS
IPR035490 GlmS/FrlB_SIS
IPR005855 GlmS_trans
IPR029055 Ntn_hydrolases_N
IPR001347 SIS
PANTHERiPTHR10937:SF0 PTHR10937:SF0, 1 hit
PfamiView protein in Pfam
PF01380 SIS, 2 hits
SUPFAMiSSF56235 SSF56235, 1 hit
TIGRFAMsiTIGR01135 glmS, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit
PS51464 SIS, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P94323-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MCGIVGILGR GPVVDKLVAS LRRLEYRGYD SAGLATLEGV RIERRRAEGK
60 70 80 90 100
LRNLEEQLRY CPPSGHAGIG HTRWATHGKP TESNAHPHAT ENVAVVHNGI
110 120 130 140 150
IENFRELRAE LERNGAGFNS ETDTEVVAHL VDSYLKNGYS PQDAVQASLP
160 170 180 190 200
RLRGAFALAF LFKANDDLLI GACKGSPLAI GHGRGEVYLG SDAIALAPLT
210 220 230 240 250
DTVTYLEDGD WAVLTRATCV IYGADGSIVQ RETSKSGVSA LLVDKANYRH
260 270 280 290 300
FMAKEIHEQP TVAGKTLAHY LDVAAKRVAL PLALPFDFNC IQRISITACG
310 320 330 340 350
TASYAGHIAK YWFERLARLP CDVDVASEFR YREAPLRRGD LAIVISQSGE
360 370 380 390 400
TADTLAALRY AKGKGLHTIS VVNVPTSTIA RESESVLPTL AGPEIGVAST
410 420 430 440 450
KAFICQLMVL GVLAVRAAKE RGKLSEIDES QLVRELIEVP RLIAAALLVE
460 470 480 490 500
PQIEKLARYI AGARTVLYLG RGTSAPLALE GALKLKEISY IHSEGYAAGE
510 520 530 540 550
LKHGPIALID EAVPVVVIAP YDEVFEKTVS NMQEVAARGG KIILITDAKG
560 570 580 590 600
ASEAMVDTLL TIVLPAMVAS FTPLVYAIPV QLLAYHTAVA RGADVDQPRN

LAKSVTVE
Length:608
Mass (Da):65,390
Last modified:January 23, 2007 - v3
Checksum:i32C12A4AB7FD1D76
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti331Y → N in AAB39494 (PubMed:9612946).Curated1
Sequence conflicti335 – 341PLRRGDL → LAPRQF in AAB39494 (PubMed:9612946).Curated7
Sequence conflicti346S → L in AAB39494 (PubMed:9612946).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA Translation: BAC46897.1
U67278 Genomic DNA Translation: AAB39494.1
RefSeqiNP_768272.1, NC_004463.1
WP_011084447.1, NZ_CP011360.1

Genome annotation databases

EnsemblBacteriaiBAC46897; BAC46897; BAC46897
GeneIDi1053361
29270959
KEGGibja:blr1632
PATRICifig|224911.44.peg.1076

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA Translation: BAC46897.1
U67278 Genomic DNA Translation: AAB39494.1
RefSeqiNP_768272.1, NC_004463.1
WP_011084447.1, NZ_CP011360.1

3D structure databases

ProteinModelPortaliP94323
SMRiP94323
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224911.blr1632

Proteomic databases

PRIDEiP94323

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC46897; BAC46897; BAC46897
GeneIDi1053361
29270959
KEGGibja:blr1632
PATRICifig|224911.44.peg.1076

Phylogenomic databases

eggNOGiENOG4105C46 Bacteria
COG0449 LUCA
HOGENOMiHOG000258896
InParanoidiP94323
KOiK00820
OMAiTSYYSGC
OrthoDBiPOG091H0061
PhylomeDBiP94323

Enzyme and pathway databases

BioCyciBDIA224911:G1G3J-1651-MONOMER

Family and domain databases

CDDicd05008 SIS_GlmS_GlmD_1, 1 hit
cd05009 SIS_GlmS_GlmD_2, 1 hit
Gene3Di3.60.20.10, 1 hit
HAMAPiMF_00164 GlmS, 1 hit
InterProiView protein in InterPro
IPR017932 GATase_2_dom
IPR035466 GlmS/AgaS_SIS
IPR035490 GlmS/FrlB_SIS
IPR005855 GlmS_trans
IPR029055 Ntn_hydrolases_N
IPR001347 SIS
PANTHERiPTHR10937:SF0 PTHR10937:SF0, 1 hit
PfamiView protein in Pfam
PF01380 SIS, 2 hits
SUPFAMiSSF56235 SSF56235, 1 hit
TIGRFAMsiTIGR01135 glmS, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit
PS51464 SIS, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiNODM_BRADU
AccessioniPrimary (citable) accession number: P94323
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 116 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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