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Protein

Cycloisomaltooligosaccharide glucanotransferase

Gene
N/A
Organism
Bacillus circulans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Produces cycloisomaltooligosaccharide from dextran containing 7, 8 or 9 glucose units. The enzyme is specific for (1->6)-alpha-D-glucans (dextrans) and, without activity toward (1->4)-alpha-D-glucans, such as amylose. It also has no activity on oligosaccharides, such as amylopectin and pullulan, containing (1->6)-alpha-D-glucosidic linkages at branch points.1 Publication

Catalytic activityi

Cyclizes part of a (1->6)-alpha-D-glucan chain by formation of a (1->6)-alpha-D-glucosidic bond.1 Publication

Kineticsi

  1. KM=0.063 mM for dextran T-401 Publication
  1. Vmax=0.872 pmol/min/µg enzyme with dextran T-40 as substrate1 Publication

pH dependencei

Optimum pH is 5.5.2 Publications

GO - Molecular functioni

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.248 649

Protein family/group databases

CAZyiCBM35 Carbohydrate-Binding Module Family 35
GH66 Glycoside Hydrolase Family 66

Names & Taxonomyi

Protein namesi
Recommended name:
Cycloisomaltooligosaccharide glucanotransferase (EC:2.4.1.248)
Short name:
CITase
OrganismiBacillus circulans
Taxonomic identifieri1397 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi183D → N: Has 1% of wild-type activity. 1 Publication1
Mutagenesisi308D → N: No activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 38Sequence analysisAdd BLAST38
ChainiPRO_000001224139 – 972Cycloisomaltooligosaccharide glucanotransferaseAdd BLAST934

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

Secondary structure

1972
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP94286
SMRiP94286
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini421 – 546CBM6 1PROSITE-ProRule annotationAdd BLAST126
Domaini748 – 871CBM6 2PROSITE-ProRule annotationAdd BLAST124

Sequence similaritiesi

Belongs to the glycosyl hydrolase 66 family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

KOiK21353

Family and domain databases

CDDicd14745 GH66, 1 hit
Gene3Di2.60.120.260, 2 hits
2.60.40.10, 1 hit
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR005084 CMB_fam6
IPR008979 Galactose-bd-like_sf
IPR025092 Glyco_hydro_66
IPR013780 Glyco_hydro_b
IPR013783 Ig-like_fold
PfamiView protein in Pfam
PF16990 CBM_35, 1 hit
PF03422 CBM_6, 1 hit
PF13199 Glyco_hydro_66, 1 hit
SUPFAMiSSF49785 SSF49785, 2 hits
PROSITEiView protein in PROSITE
PS51175 CBM6, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P94286-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVRFMYALRK RRLSLLLAMS LLVMCVASVV SPPPQALASG SGGIERVFTD
60 70 80 90 100
KARYNPGDAV SIRVQAKNGT GSSWSGAARL EIFHLENSVY TSSQSLSLTN
110 120 130 140 150
GQSTTLTFTW TAPSTDFRGY FVRIDAGTLG QGATAIDVSS DFTKYPRYGY
160 170 180 190 200
ISEFESGETA LESKAKVDQL AQDYHINAWQ FYDWMWRHDK MIKRTGGSID
210 220 230 240 250
STWLDLFNRE ISWSTLQNQI DAVHDVNGKA MAYAMIYASR ENYSPLGISP
260 270 280 290 300
TWGIYEDSSH TNQFDVDFGD GSTYLYMSDP QNPNWQNYIH AEYIDSINTA
310 320 330 340 350
GFDGIHVDQM GQRSNVYDYN GNSIDLSTRF SPFLDQAKSV LSANNPARDN
360 370 380 390 400
LTYNIVDGTV NGWAVNDVSK NADLDFLYSE IWYLSDSYNQ LKNYIEQLRA
410 420 430 440 450
NGGNKAVVLA AYMNYADNAG TRYEAESASM TNVSTNTNHA GYTGSGFVDQ
460 470 480 490 500
FASTGDKVSF AINAPEAGDY SLVFRYGNNT GANSTLNLYV DGNFVQKLYF
510 520 530 540 550
FNQSSWGTWK HDAWYQVPLT QGAHTVELRY ESGNVGAVNL DSLTLGTFDE
560 570 580 590 600
HSVRLADAMM SASGATHIEL GDDNQMLPHE YYPNRSKTMR SSLKNAMKDH
610 620 630 640 650
YNFITAYENL LFDSDVVPND TGSQFVNLTG VSASGDGSAN TVWYINKRTS
660 670 680 690 700
DYNIVHLINL LGNDNQWRNT ASQPSFQTNL PAKIYIGADE TISDVYLASP
710 720 730 740 750
DLSGGETQEL AFTSGTDAGG KYVSFTVPEL KYWNMIYMKR TFSVPANDIY
760 770 780 790 800
EAETAIKSNV STNTNHAGYT GSGFVDGFSS TNDGVSFVVK STASDDYALR
810 820 830 840 850
FRYANGGSDA TRDVYVDGKL AGTVSFKSTG SWSTWSYGEI TARLEPGHHT
860 870 880 890 900
IVLWQTSGNT GAINLDHLDL DKTYIWQFDR QIVSVPAGYR ITFRTGLPGW
910 920 930 940 950
VHWGVNGWTG VTDTPLRSNG SLDGNLDHET SIGPFATGTA VDVTFLWDDN
960 970
NNGILEPSTD RWEGTDFGIN VS
Length:972
Mass (Da):107,431
Last modified:May 1, 1997 - v1
Checksum:i2827BEA61782CC22
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D61382 Genomic DNA Translation: BAA09604.1

Genome annotation databases

KEGGiag:BAA09604

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D61382 Genomic DNA Translation: BAA09604.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WNKX-ray2.30A39-738[»]
3WNLX-ray2.60A39-738[»]
3WNMX-ray2.25A39-738[»]
3WNNX-ray2.25A/B39-738[»]
3WNOX-ray1.90A/B39-738[»]
3WNPX-ray2.80A/B39-738[»]
ProteinModelPortaliP94286
SMRiP94286
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM35 Carbohydrate-Binding Module Family 35
GH66 Glycoside Hydrolase Family 66

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:BAA09604

Phylogenomic databases

KOiK21353

Enzyme and pathway databases

BRENDAi2.4.1.248 649

Family and domain databases

CDDicd14745 GH66, 1 hit
Gene3Di2.60.120.260, 2 hits
2.60.40.10, 1 hit
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR005084 CMB_fam6
IPR008979 Galactose-bd-like_sf
IPR025092 Glyco_hydro_66
IPR013780 Glyco_hydro_b
IPR013783 Ig-like_fold
PfamiView protein in Pfam
PF16990 CBM_35, 1 hit
PF03422 CBM_6, 1 hit
PF13199 Glyco_hydro_66, 1 hit
SUPFAMiSSF49785 SSF49785, 2 hits
PROSITEiView protein in PROSITE
PS51175 CBM6, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiCTA1_BACCI
AccessioniPrimary (citable) accession number: P94286
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 1, 1997
Last modified: February 28, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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Main funding by: National Institutes of Health

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