UniProtKB - P94135 (TFDF2_CUPPJ)
Protein
Maleylacetate reductase 2
Gene
tfdFII
Organism
Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Cupriavidus necator (strain JMP 134))
Status
Functioni
Plays a major role in the degradation of chloroaromatic compounds by channeling maleylacetate and some of its substituted derivatives into the 3-oxoadipate pathway. This enzyme converts maleylacetate and 2-chloromaleylacetate with similar efficiencies. NADH is preferred to NADPH as the cosubstrate.
Catalytic activityi
Activity regulationi
Inhibited by p-chloromercuribenzoate and by 3-oxoadipate, and, in a temperature-dependent manner, by manganese.
pH dependencei
Optimum pH is 7.5.
: 3-chlorocatechol degradation Pathwayi
This protein is involved in the pathway 3-chlorocatechol degradation, which is part of Aromatic compound metabolism.View all proteins of this organism that are known to be involved in the pathway 3-chlorocatechol degradation and in Aromatic compound metabolism.
GO - Molecular functioni
- maleylacetate reductase activity Source: UniProtKB-EC
- metal ion binding Source: InterPro
GO - Biological processi
- 3-chlorocatechol catabolic process Source: CACAO
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Aromatic hydrocarbons catabolism |
Ligand | NAD |
Enzyme and pathway databases
UniPathwayi | UPA00083 |
Names & Taxonomyi
Protein namesi | Recommended name: Maleylacetate reductase 2 (EC:1.3.1.32)Alternative name(s): Chloromaleylacetate reductase Maleylacetate reductase II |
Gene namesi | Name:tfdFII Ordered Locus Names:Reut_D6471 |
Encoded oni | Plasmid pJP43 Publications Plasmid pPJ41 Publication |
Organismi | Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Cupriavidus necator (strain JMP 134)) |
Taxonomic identifieri | 264198 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000087852 | 1 – 359 | Maleylacetate reductase 2Add BLAST | 359 |
Interactioni
Subunit structurei
Homodimer.
CuratedFamily & Domainsi
Sequence similaritiesi
Belongs to the iron-containing alcohol dehydrogenase family.Curated
Phylogenomic databases
HOGENOMi | CLU_007207_0_1_4 |
OMAi | KLTVFSY |
OrthoDBi | 1456634at2 |
Family and domain databases
CDDi | cd08177, MAR, 1 hit |
InterProi | View protein in InterPro IPR001670, ADH_Fe/GldA IPR039697, Alcohol_dehydrogenase_Fe IPR034786, MAR |
PANTHERi | PTHR11496, PTHR11496, 1 hit |
Pfami | View protein in Pfam PF00465, Fe-ADH, 1 hit |
i Sequence
Sequence statusi: Complete.
P94135-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTGDLNEFVA HFWPVRVVFG AGSTERIPAE VKRLGARRAL VLCTPDQRDL
60 70 80 90 100
AQRVLGDLGD LGAGFHDGAV MHVPEASVTR AAQAARDADA DLLVAVGGGS
110 120 130 140 150
TIGLAKALAL HHGMRFVALP TTYAGSEMTP IWGLTADGAK RTGRDPRVLP
160 170 180 190 200
STVLYDPHHL TSLPPEVTGP SGMNAIAHAV ESMYAPDRNP ITMLLAEESI
210 220 230 240 250
RAMAQGLPVA VDSPGDLDAR TRTLYAAWLA GTVLGMVSMG LHHKLCHVLG
260 270 280 290 300
GRFNLPHAPM HAVLLPHVAA FNEVAAPAEL GRVAAALGAP GPGGAGAALH
310 320 330 340 350
ALLRFTCTER SLAAIGMPAQ GIYDAAEHAL ADAYANPRQA SREDIARLLR
AAFTGEMPA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U16782 Genomic DNA Translation: AAC44727.1 AY365053 Genomic DNA Translation: AAR31044.1 CP000093 Genomic DNA Translation: AAZ65769.1 |
RefSeqi | WP_011178391.1, NZ_AY365053.1 |
Genome annotation databases
EnsemblBacteriai | AAZ65769; AAZ65769; Reut_D6471 |
KEGGi | reu:Reut_D6471 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U16782 Genomic DNA Translation: AAC44727.1 AY365053 Genomic DNA Translation: AAR31044.1 CP000093 Genomic DNA Translation: AAZ65769.1 |
RefSeqi | WP_011178391.1, NZ_AY365053.1 |
3D structure databases
SMRi | P94135 |
ModBasei | Search... |
Genome annotation databases
EnsemblBacteriai | AAZ65769; AAZ65769; Reut_D6471 |
KEGGi | reu:Reut_D6471 |
Phylogenomic databases
HOGENOMi | CLU_007207_0_1_4 |
OMAi | KLTVFSY |
OrthoDBi | 1456634at2 |
Enzyme and pathway databases
UniPathwayi | UPA00083 |
Family and domain databases
CDDi | cd08177, MAR, 1 hit |
InterProi | View protein in InterPro IPR001670, ADH_Fe/GldA IPR039697, Alcohol_dehydrogenase_Fe IPR034786, MAR |
PANTHERi | PTHR11496, PTHR11496, 1 hit |
Pfami | View protein in Pfam PF00465, Fe-ADH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TFDF2_CUPPJ | |
Accessioni | P94135Primary (citable) accession number: P94135 Secondary accession number(s): Q46M61 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | May 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 118 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Plasmid, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families