Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Putative E3 ubiquitin-protein ligase SINAT1

Gene

SINAT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It probably triggers the ubiquitin-mediated degradation of different substrates (Probable). Mediates the proteasomal-dependent degradation of ATG6, a component of the autophagosome complex. Requires TRAF1A/MUSE14 and TRAF1B/MUSE13 to target ATG6 for ubiquitination and subsequent regulation of autophagosome assembly (PubMed:28351989).1 Publication1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.Curated
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi115Zinc 1By similarity1
Metal bindingi122Zinc 1By similarity1
Metal bindingi134Zinc 1By similarity1
Metal bindingi138Zinc 1By similarity1
Metal bindingi145Zinc 2By similarity1
Metal bindingi152Zinc 2By similarity1
Metal bindingi164Zinc 2By similarity1
Metal bindingi169Zinc 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri57 – 93RING-typePROSITE-ProRule annotationAdd BLAST37
Zinc fingeri110 – 170SIAH-typePROSITE-ProRule annotationAdd BLAST61

GO - Molecular functioni

  • ubiquitin protein ligase activity Source: GO_Central
  • zinc ion binding Source: InterPro

GO - Biological processi

  • multicellular organism development Source: InterPro
  • protein ubiquitination Source: UniProtKB
  • regulation of autophagosome assembly Source: UniProtKB
  • ubiquitin-dependent protein catabolic process Source: InterPro

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-5689880 Ub-specific processing proteases
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Putative E3 ubiquitin-protein ligase SINAT1Curated (EC:2.3.2.271 Publication)
Alternative name(s):
RING-type E3 ubiquitin transferase SINAT1Curated
Seven in absentia homolog 1Curated
Gene namesi
Name:SINAT11 Publication
Ordered Locus Names:At2g41980
ORF Names:T6D20.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G41980
TAIRilocus:2064642 AT2G41980

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000561801 – 305Putative E3 ubiquitin-protein ligase SINAT1Add BLAST305

Proteomic databases

PaxDbiP93748

PTM databases

iPTMnetiP93748

Expressioni

Gene expression databases

ExpressionAtlasiP93748 baseline and differential
GenevisibleiP93748 AT

Interactioni

Subunit structurei

Interacts with SINAT6 (PubMed:24350984). Interacts with ATG6 and TRAF1A (PubMed:28351989). Interacts with WAV3 (PubMed:22122664).3 Publications

Protein-protein interaction databases

STRINGi3702.AT2G41980.1

Structurei

3D structure databases

ProteinModelPortaliP93748
SMRiP93748
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni107 – 300SBDAdd BLAST194

Domaini

The RING-type zinc finger domain is essential for ubiquitin ligase activity.By similarity
The SBD domain (substrate-binding domain) mediates the homodimerization and the interaction with substrate proteins. It is related to the TRAF family.By similarity

Sequence similaritiesi

Belongs to the SINA (Seven in absentia) family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri57 – 93RING-typePROSITE-ProRule annotationAdd BLAST37
Zinc fingeri110 – 170SIAH-typePROSITE-ProRule annotationAdd BLAST61

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3002 Eukaryota
ENOG410XVP0 LUCA
HOGENOMiHOG000231487
InParanoidiP93748
KOiK04506
OMAiTEIHPYK
OrthoDBiEOG09360EO3
PhylomeDBiP93748

Family and domain databases

Gene3Di2.60.210.10, 1 hit
3.30.40.10, 2 hits
InterProiView protein in InterPro
IPR018121 7-in-absentia-prot_TRAF-dom
IPR004162 SINA-like
IPR008974 TRAF-like
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR013010 Znf_SIAH
PANTHERiPTHR10315 PTHR10315, 1 hit
PfamiView protein in Pfam
PF03145 Sina, 1 hit
SUPFAMiSSF49599 SSF49599, 1 hit
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit
PS51081 ZF_SIAH, 1 hit

Sequencei

Sequence statusi: Complete.

P93748-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAPGGSALKE ALESNSTGVD YEVKMAKVEA NSKPTKSGSG SIGKFHSSNG
60 70 80 90 100
VYELLECPVC TNLMYPPIHQ CPNGHTLCSS CKLRVQNTCP TCRYELGNIR
110 120 130 140 150
CLALEKVAES LEVPCRYQNL GCQDIFPYYS KLKHEQHCRF RSYSCPYAGS
160 170 180 190 200
ECSVTGDIPT LVDHLKDDHK VDMHDGCTFN HRYVKSNPHE VENATWMLTV
210 220 230 240 250
FNCFGRQFCL HFEAFQLGMA PVYMAFLRFM GDENEAKKFS YSLEVGAHSR
260 270 280 290 300
KLTWQGIPRS IRDSHRKVRD SQDGLIIPRN LALYFSGSDK EELKLRVTGR

IWKEE
Length:305
Mass (Da):34,557
Last modified:May 1, 1997 - v1
Checksum:i85E183DD5985E116
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90439 Genomic DNA Translation: AAB63545.1
CP002685 Genomic DNA Translation: AEC10058.1
PIRiE84848
RefSeqiNP_181729.1, NM_129762.6
UniGeneiAt.53097

Genome annotation databases

EnsemblPlantsiAT2G41980.1; AT2G41980.1; AT2G41980
GeneIDi818798
GrameneiAT2G41980.1; AT2G41980.1; AT2G41980
KEGGiath:AT2G41980

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90439 Genomic DNA Translation: AAB63545.1
CP002685 Genomic DNA Translation: AEC10058.1
PIRiE84848
RefSeqiNP_181729.1, NM_129762.6
UniGeneiAt.53097

3D structure databases

ProteinModelPortaliP93748
SMRiP93748
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G41980.1

PTM databases

iPTMnetiP93748

Proteomic databases

PaxDbiP93748

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G41980.1; AT2G41980.1; AT2G41980
GeneIDi818798
GrameneiAT2G41980.1; AT2G41980.1; AT2G41980
KEGGiath:AT2G41980

Organism-specific databases

AraportiAT2G41980
TAIRilocus:2064642 AT2G41980

Phylogenomic databases

eggNOGiKOG3002 Eukaryota
ENOG410XVP0 LUCA
HOGENOMiHOG000231487
InParanoidiP93748
KOiK04506
OMAiTEIHPYK
OrthoDBiEOG09360EO3
PhylomeDBiP93748

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-ATH-5689880 Ub-specific processing proteases
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

PROiPR:P93748

Gene expression databases

ExpressionAtlasiP93748 baseline and differential
GenevisibleiP93748 AT

Family and domain databases

Gene3Di2.60.210.10, 1 hit
3.30.40.10, 2 hits
InterProiView protein in InterPro
IPR018121 7-in-absentia-prot_TRAF-dom
IPR004162 SINA-like
IPR008974 TRAF-like
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR013010 Znf_SIAH
PANTHERiPTHR10315 PTHR10315, 1 hit
PfamiView protein in Pfam
PF03145 Sina, 1 hit
SUPFAMiSSF49599 SSF49599, 1 hit
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit
PS51081 ZF_SIAH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSINA1_ARATH
AccessioniPrimary (citable) accession number: P93748
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: May 1, 1997
Last modified: November 7, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again