UniProtKB - P93028 (UBE11_ARATH)
Protein
Ubiquitin-activating enzyme E1 1
Gene
UBA1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP.2 Publications
Miscellaneous
Both UBA1 and UBA2 are able to activate ubiquitin and transfer it to the E2s with equal efficiency.1 Publication
Mutation in UBA1 (mos5) suppresses snc1-mediated constitutive resistance and affects the resistance responses conferred by only a subset of R-proteins.1 Publication
Catalytic activityi
- ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine.1 Publication EC:6.2.1.45
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 PublicationView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 502 | ATP; via amide nitrogenBy similarity | 1 | |
Binding sitei | 528 | ATPBy similarity | 1 | |
Binding sitei | 539 | ATPBy similarity | 1 | |
Binding sitei | 552 | ATPBy similarity | 1 | |
Active sitei | 656 | Glycyl thioester intermediatePROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 600 – 601 | ATPBy similarity | 2 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ubiquitin activating enzyme activity Source: GO_Central
- ubiquitin-protein transferase activity Source: TAIR
GO - Biological processi
- cellular response to DNA damage stimulus Source: GO_Central
- protein modification by small protein conjugation Source: GO_Central
- protein ubiquitination Source: TAIR
- response to cadmium ion Source: TAIR
- response to other organism Source: TAIR
- ubiquitin-dependent protein catabolic process Source: GO_Central
Keywordsi
Molecular function | Ligase |
Biological process | Ubl conjugation pathway |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 6.2.1.B9, 399 |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin-activating enzyme E1 1 (EC:6.2.1.451 Publication)Short name: AtUBA11 Publication Alternative name(s): Protein MODIFIER OF SNC1 5 |
Gene namesi | Name:UBA1 Synonyms:MOS5 Ordered Locus Names:At2g30110 ORF Names:T27E13.15 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G30110 |
TAIRi | locus:2060854, AT2G30110 |
Subcellular locationi
Cytosol
- cytosol Source: TAIR
Nucleus
- nucleus Source: GO_Central
Peroxisome
- peroxisome Source: TAIR
Plasma Membrane
- plasma membrane Source: TAIR
Other locations
- cytoplasm Source: GO_Central
- plasmodesma Source: TAIR
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 1032 – 1037 | RMDKKV → S in mos5; enhanced disease susceptibility. 1 Publication | 6 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000399395 | 1 – 1080 | Ubiquitin-activating enzyme E1 1Add BLAST | 1080 |
Proteomic databases
PaxDbi | P93028 |
PRIDEi | P93028 |
ProteomicsDBi | 243240 |
PTM databases
iPTMneti | P93028 |
Expressioni
Tissue specificityi
Expressed in leaves, flowers, roots and stems. Detected in germinating seeds, cotyledons, hypocotyls, vascular tissues, anthers, filaments, pollen, style, stigma, sepals, petals, ovary, developing ovules, funiculi and silique walls.1 Publication
Developmental stagei
Expressed over the entire range of development.1 Publication
Gene expression databases
ExpressionAtlasi | P93028, baseline and differential |
Genevisiblei | P93028, AT |
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
BioGRIDi | 2911, 2 interactors |
IntActi | P93028, 1 interactor |
STRINGi | 3702.AT2G30110.1 |
Family & Domainsi
Sequence similaritiesi
Belongs to the ubiquitin-activating E1 family.Curated
Phylogenomic databases
eggNOGi | KOG2012, Eukaryota |
HOGENOMi | CLU_002556_0_0_1 |
InParanoidi | P93028 |
OMAi | THEYEAV |
OrthoDBi | 91748at2759 |
PhylomeDBi | P93028 |
Family and domain databases
Gene3Di | 1.10.10.2660, 1 hit 2.40.30.180, 1 hit 3.10.290.60, 1 hit 3.50.50.80, 1 hit |
InterProi | View protein in InterPro IPR032420, E1_4HB IPR032418, E1_FCCH IPR042302, E1_FCCH_sf IPR000594, ThiF_NAD_FAD-bd IPR018965, Ub-activating_enz_E1_C IPR042449, Ub-E1_IAD_1 IPR038252, UBA_E1_C_sf IPR019572, UBA_E1_Cys IPR042063, Ubi_acti_E1_SCCH IPR035985, Ubiquitin-activating_enz IPR018075, UBQ-activ_enz_E1 IPR018074, UBQ-activ_enz_E1_CS IPR033127, UBQ-activ_enz_E1_Cys_AS IPR000011, UBQ/SUMO-activ_enz_E1-like |
Pfami | View protein in Pfam PF16191, E1_4HB, 1 hit PF16190, E1_FCCH, 1 hit PF09358, E1_UFD, 1 hit PF00899, ThiF, 2 hits PF10585, UBA_e1_thiolCys, 1 hit |
PRINTSi | PR01849, UBIQUITINACT |
SMARTi | View protein in SMART SM00985, UBA_e1_C, 1 hit |
SUPFAMi | SSF69572, SSF69572, 2 hits |
TIGRFAMsi | TIGR01408, Ube1, 1 hit |
PROSITEi | View protein in PROSITE PS00536, UBIQUITIN_ACTIVAT_1, 1 hit PS00865, UBIQUITIN_ACTIVAT_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P93028-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLHKRASEAN DKNDNTIIGS DLASSKKRRI DFTESSSDKS SSILASGSSR
60 70 80 90 100
GFHGDSVVQQ IDMAFGNSNR QEIDEDLHSR QLAVYGRETM RRLFASNVLI
110 120 130 140 150
SGMHGLGAEI AKNLILAGVK SVTLHDERVV ELWDLSSNFV FSEDDVGKNR
160 170 180 190 200
ADASVQKLQD LNNAVVVSSL TKSLNKEDLS GFQVVVFSDI SMERAIEFDD
210 220 230 240 250
YCHSHQPPIA FVKADVRGLF GSVFCDFGPE FAVLDVDGEE PHTGIIASIS
260 270 280 290 300
NENQAFISCV DDERLEFEDG DLVVFSEVEG MTELNDGKPR KIKSTRPYSF
310 320 330 340 350
TLDEDTTNYG TYVKGGIVTQ VKQPKLLNFK PLREALKDPG DFLFSDFSKF
360 370 380 390 400
DRPPLLHLAF QALDHFKAEA GRFPVAGSEE DAQKLISIAT AINTGQGDLK
410 420 430 440 450
VENVDQKLLR HFSFGAKAVL NPMAAMFGGI VGQEVVKACS GKFHPLFQFF
460 470 480 490 500
YFDSVESLPS EPVDSSDFAP RNSRYDAQIS VFGAKFQKKL EDAKVFTVGS
510 520 530 540 550
GALGCEFLKN LALMGVSCGS QGKLTVTDDD IIEKSNLSRQ FLFRDWNIGQ
560 570 580 590 600
AKSTVAASAA AVINPRFNIE ALQNRVGAET ENVFDDAFWE NLTVVVNALD
610 620 630 640 650
NVNARLYVDS RCLYFQKPLL ESGTLGTKCN TQSVIPHLTE NYGASRDPPE
660 670 680 690 700
KQAPMCTVHS FPHNIDHCLT WARSEFEGLL EKTPAEVNAY LSSPVEYTNS
710 720 730 740 750
MMSAGDAQAR DTLERIVECL EKEKCETFQD CLTWARLRFE DYFVNRVKQL
760 770 780 790 800
IYTFPEDAAT STGAPFWSAP KRFPRPLQYS SSDPSLLNFI TATAILRAET
810 820 830 840 850
FGIPIPEWTK NPKEAAEAVD RVIVPDFEPR QDAKIVTDEK ATTLTTASVD
860 870 880 890 900
DAAVIDDLIA KIDQCRHNLS PDFRMKPIQF EKDDDTNYHM DVIAGLANMR
910 920 930 940 950
ARNYSIPEVD KLKAKFIAGR IIPAIATSTA MATGLVCLEL YKVLDGGHKV
960 970 980 990 1000
EAYRNTFANL ALPLFSMAEP LPPKVVKHRD MAWTVWDRWV LKGNPTLREV
1010 1020 1030 1040 1050
LQWLEDKGLS AYSISCGSCL LFNSMFTRHK ERMDKKVVDL ARDVAKVELP
1060 1070 1080
PYRNHLDVVV ACEDEDDNDV DIPLVSIYFR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 883 | D → G in AAP21171 (PubMed:14593172).Curated | 1 | |
Sequence conflicti | 883 | D → G in AAL90910 (PubMed:14593172).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U80808 Genomic DNA Translation: AAB39246.1 AC004165 Genomic DNA Translation: AAC16961.1 CP002685 Genomic DNA Translation: AEC08346.1 AY090248 mRNA Translation: AAL90910.1 BT006363 mRNA Translation: AAP21171.1 |
PIRi | T00587 |
RefSeqi | NP_565693.1, NM_128566.4 |
Genome annotation databases
EnsemblPlantsi | AT2G30110.1; AT2G30110.1; AT2G30110 |
GeneIDi | 817562 |
Gramenei | AT2G30110.1; AT2G30110.1; AT2G30110 |
KEGGi | ath:AT2G30110 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U80808 Genomic DNA Translation: AAB39246.1 AC004165 Genomic DNA Translation: AAC16961.1 CP002685 Genomic DNA Translation: AEC08346.1 AY090248 mRNA Translation: AAL90910.1 BT006363 mRNA Translation: AAP21171.1 |
PIRi | T00587 |
RefSeqi | NP_565693.1, NM_128566.4 |
3D structure databases
SMRi | P93028 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 2911, 2 interactors |
IntActi | P93028, 1 interactor |
STRINGi | 3702.AT2G30110.1 |
PTM databases
iPTMneti | P93028 |
Proteomic databases
PaxDbi | P93028 |
PRIDEi | P93028 |
ProteomicsDBi | 243240 |
Genome annotation databases
EnsemblPlantsi | AT2G30110.1; AT2G30110.1; AT2G30110 |
GeneIDi | 817562 |
Gramenei | AT2G30110.1; AT2G30110.1; AT2G30110 |
KEGGi | ath:AT2G30110 |
Organism-specific databases
Araporti | AT2G30110 |
TAIRi | locus:2060854, AT2G30110 |
Phylogenomic databases
eggNOGi | KOG2012, Eukaryota |
HOGENOMi | CLU_002556_0_0_1 |
InParanoidi | P93028 |
OMAi | THEYEAV |
OrthoDBi | 91748at2759 |
PhylomeDBi | P93028 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
BRENDAi | 6.2.1.B9, 399 |
Miscellaneous databases
PROi | PR:P93028 |
Gene expression databases
ExpressionAtlasi | P93028, baseline and differential |
Genevisiblei | P93028, AT |
Family and domain databases
Gene3Di | 1.10.10.2660, 1 hit 2.40.30.180, 1 hit 3.10.290.60, 1 hit 3.50.50.80, 1 hit |
InterProi | View protein in InterPro IPR032420, E1_4HB IPR032418, E1_FCCH IPR042302, E1_FCCH_sf IPR000594, ThiF_NAD_FAD-bd IPR018965, Ub-activating_enz_E1_C IPR042449, Ub-E1_IAD_1 IPR038252, UBA_E1_C_sf IPR019572, UBA_E1_Cys IPR042063, Ubi_acti_E1_SCCH IPR035985, Ubiquitin-activating_enz IPR018075, UBQ-activ_enz_E1 IPR018074, UBQ-activ_enz_E1_CS IPR033127, UBQ-activ_enz_E1_Cys_AS IPR000011, UBQ/SUMO-activ_enz_E1-like |
Pfami | View protein in Pfam PF16191, E1_4HB, 1 hit PF16190, E1_FCCH, 1 hit PF09358, E1_UFD, 1 hit PF00899, ThiF, 2 hits PF10585, UBA_e1_thiolCys, 1 hit |
PRINTSi | PR01849, UBIQUITINACT |
SMARTi | View protein in SMART SM00985, UBA_e1_C, 1 hit |
SUPFAMi | SSF69572, SSF69572, 2 hits |
TIGRFAMsi | TIGR01408, Ube1, 1 hit |
PROSITEi | View protein in PROSITE PS00536, UBIQUITIN_ACTIVAT_1, 1 hit PS00865, UBIQUITIN_ACTIVAT_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBE11_ARATH | |
Accessioni | P93028Primary (citable) accession number: P93028 Secondary accession number(s): Q8RX82 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 5, 2010 |
Last sequence update: | May 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 156 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families