UniProtKB - P93025 (PHOT2_ARATH)
Protein
Phototropin-2
Gene
PHOT2
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Protein kinase that acts as a blue light photoreceptor in a signal-transduction pathway for photo-induced movements. Phosphorylates BLUS1, a kinase involved in stomatal opening. Mediates calcium spiking of extra- and intracellular origins in response to blue light. Involved in hypocotyl phototropism. Contributes to the chloroplast accumulation in low blue light and mediates their translocation (avoidance response) at high fluence. Regulates stomata opening and photomorphogenesis response of leaf tissue. Not involved in hypocotyl elongation inhibition, anthocyanin accumulation or cotyledon opening.6 Publications
Miscellaneous
Undergoes a photocycle characterized by fluorescence and absorption changes induced by blue light. Half-time of photoproduct formation is 11 seconds and 15 seconds for dark regeneration.
Catalytic activityi
Cofactori
FMNCuratedNote: Binds 2 FMN per subunit.Curated
Absorptioni
Abs(max)=450 nm1 PublicationExhibits a smaller absorbance peak at 350 nm. The broad fluorescence emission spectrum peaks at 490 nm.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 187 | FMN1 Publication | 1 | |
Binding sitei | 202 | FMN1 Publication | 1 | |
Binding sitei | 212 | FMN1 Publication | 1 | |
Binding sitei | 233 | FMN1 Publication | 1 | |
Binding sitei | 443 | FMNBy similarity | 1 | |
Binding sitei | 458 | FMNBy similarity | 1 | |
Binding sitei | 468 | FMNBy similarity | 1 | |
Binding sitei | 489 | FMNBy similarity | 1 | |
Binding sitei | 606 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 702 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 169 – 174 | FMN1 Publication | 6 | |
Nucleotide bindingi | 425 – 430 | FMNBy similarity | 6 | |
Nucleotide bindingi | 583 – 591 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- blue light photoreceptor activity Source: TAIR
- FMN binding Source: UniProtKB
- identical protein binding Source: UniProtKB
- kinase activity Source: TAIR
- protein serine/threonine kinase activity Source: TAIR
GO - Biological processi
- chloroplast relocation Source: TAIR
- circadian rhythm Source: TAIR
- negative regulation of anion channel activity by blue light Source: TAIR
- phototropism Source: TAIR
- protein autophosphorylation Source: TAIR
- protein-chromophore linkage Source: UniProtKB-KW
- protein phosphorylation Source: GO_Central
- response to blue light Source: TAIR
- stomatal movement Source: TAIR
Keywordsi
Molecular function | Kinase, Photoreceptor protein, Receptor, Serine/threonine-protein kinase, Transferase |
Biological process | Sensory transduction |
Ligand | ATP-binding, Chromophore, Flavoprotein, FMN, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Phototropin-2 (EC:2.7.11.1)Alternative name(s): Defective in chloroplast avoidance protein 1 Non-phototropic hypocotyl 1-like protein 1 Short name: AtKin7 Short name: NPH1-like protein 1 |
Gene namesi | Name:PHOT2 Synonyms:CAV1, KIN7, NPL1 Ordered Locus Names:At5g58140 ORF Names:K21L19.6 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G58140 |
TAIRi | locus:2155821, AT5G58140 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Peripheral membrane protein 1 Publication
Golgi apparatus
- Golgi apparatus Source: TAIR
Nucleus
- nucleus Source: GO_Central
Plasma Membrane
- plasma membrane Source: TAIR
Other locations
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 170 | C → A: No effect on light-dependent autophosphorylation. 1 Publication | 1 | |
Mutagenesisi | 217 | T → Q: Loss of dimerization. 1 Publication | 1 | |
Mutagenesisi | 232 | M → V: Loss of dimerization. 1 Publication | 1 | |
Mutagenesisi | 426 | C → A: Severe loss of light-sensing and light-dependent autophosphorylation. 1 Publication | 1 | |
Mutagenesisi | 727 | T → I in cav1-2; loss of chloroplast avoidance response in response to high fluence blue light. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086523 | 1 – 915 | Phototropin-2Add BLAST | 915 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 9 | PhosphoserineCombined sources | 1 | |
Modified residuei | 22 | PhosphoserineCombined sources | 1 | |
Modified residuei | 121 | PhosphoserineBy similarity | 1 | |
Modified residuei | 170 | S-4a-FMN cysteineBy similarity | 1 | |
Modified residuei | 364 | PhosphoserineCombined sources | 1 | |
Modified residuei | 426 | S-4a-FMN cysteineBy similarity | 1 |
Post-translational modificationi
Autophosphorylated in response to blue light irradiation.1 Publication
2 molecules of FMN bind covalently to cysteines after exposure to blue light and are reversed in the dark.By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P93025 |
PRIDEi | P93025 |
ProteomicsDBi | 235062 [P93025-1] |
PTM databases
iPTMneti | P93025 |
Expressioni
Tissue specificityi
Expressed in leaves, stems and flowers, and to a lower extent in roots.1 Publication
Inductioni
Light fluence rate-dependent induction, independent of light quality.1 Publication
Gene expression databases
ExpressionAtlasi | P93025, baseline and differential |
Genevisiblei | P93025, AT |
Interactioni
Subunit structurei
Homodimer.
Interacts with PKS1, PKS2, RPT3 and PHOT1.
2 PublicationsBinary interactionsi
Hide detailsP93025
With | #Exp. | IntAct |
---|---|---|
COP1 [P43254] | 2 | EBI-2270423,EBI-301649 |
itself | 2 | EBI-2270423,EBI-2270423 |
GO - Molecular functioni
- identical protein binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 21170, 11 interactors |
DIPi | DIP-53468N |
IntActi | P93025, 4 interactors |
MINTi | P93025 |
STRINGi | 3702.AT5G58140.2 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P93025 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P93025 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 120 – 193 | PAS 1PROSITE-ProRule annotationAdd BLAST | 74 | |
Domaini | 194 – 248 | PAC 1PROSITE-ProRule annotationAdd BLAST | 55 | |
Domaini | 376 – 449 | PAS 2PROSITE-ProRule annotationAdd BLAST | 74 | |
Domaini | 450 – 504 | PAC 2PROSITE-ProRule annotationAdd BLAST | 55 | |
Domaini | 577 – 864 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 288 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 720 – 774 | Activation loopBy similarityAdd BLAST | 55 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 16 – 114 | Ser-richAdd BLAST | 99 |
Domaini
The activation loop within the kinase domain is the target of phosphorylation (By similarity). The PAS (PER-ARNT-SIM) domains are required for the binding of FMN chromophores.By similarity
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | ENOG502QPPH, Eukaryota |
HOGENOMi | CLU_006321_0_1_1 |
InParanoidi | P93025 |
PhylomeDBi | P93025 |
Family and domain databases
CDDi | cd00130, PAS, 2 hits |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR001610, PAC IPR000014, PAS IPR000700, PAS-assoc_C IPR035965, PAS-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF13426, PAS_9, 2 hits PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00086, PAC, 2 hits SM00091, PAS, 2 hits SM00220, S_TKc, 1 hit |
SUPFAMi | SSF55785, SSF55785, 2 hits SSF56112, SSF56112, 1 hit |
TIGRFAMsi | TIGR00229, sensory_box, 2 hits |
PROSITEi | View protein in PROSITE PS50113, PAC, 2 hits PS50112, PAS, 2 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P93025-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MERPRAPPSP LNDAESLSER RSLEIFNPSS GKETHGSTSS SSKPPLDGNN
60 70 80 90 100
KGSSSKWMEF QDSAKITERT AEWGLSAVKP DSGDDGISFK LSSEVERSKN
110 120 130 140 150
MSRRSSEEST SSESGAFPRV SQELKTALST LQQTFVVSDA TQPHCPIVYA
160 170 180 190 200
SSGFFTMTGY SSKEIVGRNC RFLQGPDTDK NEVAKIRDCV KNGKSYCGRL
210 220 230 240 250
LNYKKDGTPF WNLLTVTPIK DDQGNTIKFI GMQVEVSKYT EGVNDKALRP
260 270 280 290 300
NGLSKSLIRY DARQKEKALD SITEVVQTIR HRKSQVQESV SNDTMVKPDS
310 320 330 340 350
STTPTPGRQT RQSDEASKSF RTPGRVSTPT GSKLKSSNNR HEDLLRMEPE
360 370 380 390 400
ELMLSTEVIG QRDSWDLSDR ERDIRQGIDL ATTLERIEKN FVISDPRLPD
410 420 430 440 450
NPIIFASDSF LELTEYSREE ILGRNCRFLQ GPETDQATVQ KIRDAIRDQR
460 470 480 490 500
EITVQLINYT KSGKKFWNLF HLQPMRDQKG ELQYFIGVQL DGSDHVEPLQ
510 520 530 540 550
NRLSERTEMQ SSKLVKATAT NVDEAVRELP DANTRPEDLW AAHSKPVYPL
560 570 580 590 600
PHNKESTSWK AIKKIQASGE TVGLHHFKPI KPLGSGDTGS VHLVELKGTG
610 620 630 640 650
ELYAMKAMEK TMMLNRNKAH RACIEREIIS LLDHPFLPTL YASFQTSTHV
660 670 680 690 700
CLITDFCPGG ELFALLDRQP MKILTEDSAR FYAAEVVIGL EYLHCLGIVY
710 720 730 740 750
RDLKPENILL KKDGHIVLAD FDLSFMTTCT PQLIIPAAPS KRRRSKSQPL
760 770 780 790 800
PTFVAEPSTQ SNSFVGTEEY IAPEIITGAG HTSAIDWWAL GILLYEMLYG
810 820 830 840 850
RTPFRGKNRQ KTFANILHKD LTFPSSIPVS LVGRQLINTL LNRDPSSRLG
860 870 880 890 900
SKGGANEIKQ HAFFRGINWP LIRGMSPPPL DAPLSIIEKD PNAKDIKWED
910
DGVLVNSTDL DIDLF
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA8MS49 | A8MS49_ARATH | Phototropin 2 | PHOT2 AtPHOT2, NPL1, phototropin 2, At5g58140, K21L19.6 | 898 | Annotation score: | ||
F4KDJ3 | F4KDJ3_ARATH | Phototropin 2 | PHOT2 AtPHOT2, NPL1, phototropin 2, At5g58140, K21L19.6 | 689 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 722 | D → Y in AAB39188 (Ref. 5) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_016306 | 535 – 549 | RPEDL…KPVYP → VRCTSSLLLNKDGKV in isoform 2. 1 PublicationAdd BLAST | 15 | |
Alternative sequenceiVSP_016307 | 550 – 915 | Missing in isoform 2. 1 PublicationAdd BLAST | 366 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF053941 mRNA Translation: AAC27293.2 AB019228, AB024029 Genomic DNA Translation: BAB09904.1 CP002688 Genomic DNA Translation: AED97002.1 CP002688 Genomic DNA Translation: AED97004.1 CP002688 Genomic DNA Translation: ANM68619.1 CP002688 Genomic DNA Translation: ANM68620.1 CP002688 Genomic DNA Translation: ANM68621.1 AY093141 mRNA Translation: AAM13140.1 BT008901 mRNA Translation: AAP68340.1 U79744 mRNA Translation: AAB39188.1 |
PIRi | T51600 |
RefSeqi | NP_001318824.1, NM_001345288.1 [P93025-1] NP_001330355.1, NM_001345289.1 [P93025-1] NP_001330356.1, NM_001345290.1 [P93025-1] NP_851210.1, NM_180879.2 [P93025-1] NP_851211.1, NM_180880.2 [P93025-1] |
Genome annotation databases
EnsemblPlantsi | AT5G58140.1; AT5G58140.1; AT5G58140 [P93025-1] AT5G58140.2; AT5G58140.2; AT5G58140 [P93025-1] AT5G58140.5; AT5G58140.5; AT5G58140 [P93025-1] AT5G58140.6; AT5G58140.6; AT5G58140 [P93025-1] AT5G58140.7; AT5G58140.7; AT5G58140 [P93025-1] |
GeneIDi | 835926 |
Gramenei | AT5G58140.1; AT5G58140.1; AT5G58140 [P93025-1] AT5G58140.2; AT5G58140.2; AT5G58140 [P93025-1] AT5G58140.5; AT5G58140.5; AT5G58140 [P93025-1] AT5G58140.6; AT5G58140.6; AT5G58140 [P93025-1] AT5G58140.7; AT5G58140.7; AT5G58140 [P93025-1] |
KEGGi | ath:AT5G58140 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF053941 mRNA Translation: AAC27293.2 AB019228, AB024029 Genomic DNA Translation: BAB09904.1 CP002688 Genomic DNA Translation: AED97002.1 CP002688 Genomic DNA Translation: AED97004.1 CP002688 Genomic DNA Translation: ANM68619.1 CP002688 Genomic DNA Translation: ANM68620.1 CP002688 Genomic DNA Translation: ANM68621.1 AY093141 mRNA Translation: AAM13140.1 BT008901 mRNA Translation: AAP68340.1 U79744 mRNA Translation: AAB39188.1 |
PIRi | T51600 |
RefSeqi | NP_001318824.1, NM_001345288.1 [P93025-1] NP_001330355.1, NM_001345289.1 [P93025-1] NP_001330356.1, NM_001345290.1 [P93025-1] NP_851210.1, NM_180879.2 [P93025-1] NP_851211.1, NM_180880.2 [P93025-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2Z6D | X-ray | 2.00 | A/B | 117-246 | [»] | |
4EEP | X-ray | 1.70 | A | 385-496 | [»] | |
4EER | X-ray | 1.75 | A | 385-496 | [»] | |
4EES | X-ray | 1.80 | A | 385-496 | [»] | |
4EET | X-ray | 1.20 | B/D | 385-496 | [»] | |
4EEU | X-ray | 1.41 | A | 385-496 | [»] | |
4NXB | X-ray | 2.56 | A/B | 388-496 | [»] | |
4NXE | X-ray | 2.10 | A/B | 388-496 | [»] | |
4NXF | X-ray | 1.77 | A/B | 388-496 | [»] | |
4NXG | X-ray | 2.09 | A/B | 388-496 | [»] | |
6GPU | X-ray | 1.17 | A | 387-492 | [»] | |
6GPV | X-ray | 2.00 | A | 387-492 | [»] | |
6QQH | X-ray | 1.38 | A | 387-507 | [»] | |
6QQI | X-ray | 1.70 | A | 387-507 | [»] | |
6QQJ | X-ray | 2.08 | A | 387-507 | [»] | |
6QQK | X-ray | 2.40 | A | 387-507 | [»] | |
6QSA | X-ray | 1.70 | A | 387-507 | [»] | |
6S45 | X-ray | 2.20 | A | 387-492 | [»] | |
6S46 | X-ray | 2.75 | A | 387-492 | [»] | |
SMRi | P93025 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 21170, 11 interactors |
DIPi | DIP-53468N |
IntActi | P93025, 4 interactors |
MINTi | P93025 |
STRINGi | 3702.AT5G58140.2 |
PTM databases
iPTMneti | P93025 |
Proteomic databases
PaxDbi | P93025 |
PRIDEi | P93025 |
ProteomicsDBi | 235062 [P93025-1] |
Genome annotation databases
EnsemblPlantsi | AT5G58140.1; AT5G58140.1; AT5G58140 [P93025-1] AT5G58140.2; AT5G58140.2; AT5G58140 [P93025-1] AT5G58140.5; AT5G58140.5; AT5G58140 [P93025-1] AT5G58140.6; AT5G58140.6; AT5G58140 [P93025-1] AT5G58140.7; AT5G58140.7; AT5G58140 [P93025-1] |
GeneIDi | 835926 |
Gramenei | AT5G58140.1; AT5G58140.1; AT5G58140 [P93025-1] AT5G58140.2; AT5G58140.2; AT5G58140 [P93025-1] AT5G58140.5; AT5G58140.5; AT5G58140 [P93025-1] AT5G58140.6; AT5G58140.6; AT5G58140 [P93025-1] AT5G58140.7; AT5G58140.7; AT5G58140 [P93025-1] |
KEGGi | ath:AT5G58140 |
Organism-specific databases
Araporti | AT5G58140 |
TAIRi | locus:2155821, AT5G58140 |
Phylogenomic databases
eggNOGi | ENOG502QPPH, Eukaryota |
HOGENOMi | CLU_006321_0_1_1 |
InParanoidi | P93025 |
PhylomeDBi | P93025 |
Miscellaneous databases
EvolutionaryTracei | P93025 |
PROi | PR:P93025 |
Gene expression databases
ExpressionAtlasi | P93025, baseline and differential |
Genevisiblei | P93025, AT |
Family and domain databases
CDDi | cd00130, PAS, 2 hits |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR001610, PAC IPR000014, PAS IPR000700, PAS-assoc_C IPR035965, PAS-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF13426, PAS_9, 2 hits PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00086, PAC, 2 hits SM00091, PAS, 2 hits SM00220, S_TKc, 1 hit |
SUPFAMi | SSF55785, SSF55785, 2 hits SSF56112, SSF56112, 1 hit |
TIGRFAMsi | TIGR00229, sensory_box, 2 hits |
PROSITEi | View protein in PROSITE PS50113, PAC, 2 hits PS50112, PAS, 2 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PHOT2_ARATH | |
Accessioni | P93025Primary (citable) accession number: P93025 Secondary accession number(s): O81204, Q8RWE6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 22, 2005 |
Last sequence update: | November 22, 2005 | |
Last modified: | December 2, 2020 | |
This is version 176 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families