UniProtKB - P92980 (APR3_ARATH)
Protein
5'-adenylylsulfate reductase 3, chloroplastic
Gene
APR3
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Reduces sulfate for Cys biosynthesis. Substrate preference is adenosine-5'-phosphosulfate (APS) >> 3'-phosphoadenosine-5'-phosphosulfate (PAPS). Uses glutathione or DTT as source of protons.
Catalytic activityi
- EC:1.8.4.9
Cofactori
[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity
Activity regulationi
Stimulated by sodium sulfate > ammonium sulfate.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 378 | NucleophileBy similarity | 1 | |
Active sitei | 381 | NucleophileBy similarity | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- adenylyl-sulfate reductase (glutathione) activity Source: UniProtKB-EC
- adenylyl-sulfate reductase activity Source: TAIR
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- cysteine biosynthetic process Source: UniProtKB-KW
- sulfate assimilation Source: TAIR
- sulfate reduction Source: InterPro
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Amino-acid biosynthesis, Cysteine biosynthesis, Stress response |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:AT4G21990-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: 5'-adenylylsulfate reductase 3, chloroplastic (EC:1.8.4.9)Alternative name(s): 3'-phosphoadenosine-5'-phosphosulfate reductase homolog 26 Short name: PAPS reductase homolog 26 Short name: Prh-26 Adenosine 5'-phosphosulfate 5'-adenylylsulfate sulfotransferase 3 Short name: APS sulfotransferase 3 Thioredoxin-independent APS reductase 3 |
Gene namesi | Name:APR3 Synonyms:PRH26 Ordered Locus Names:At4g21990 ORF Names:F1N20.90 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G21990 |
TAIRi | locus:2120628, AT4G21990 |
Subcellular locationi
Chloroplast
- chloroplast Curated
Chloroplast
- chloroplast Source: TAIR
Keywords - Cellular componenti
Chloroplast, PlastidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 69 | ChloroplastSequence analysisAdd BLAST | 69 | |
ChainiPRO_0000023216 | 70 – 458 | 5'-adenylylsulfate reductase 3, chloroplasticAdd BLAST | 389 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 378 ↔ 381 | Redox-activePROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bondProteomic databases
PaxDbi | P92980 |
PRIDEi | P92980 |
ProteomicsDBi | 244440 [P92980-1] |
Expressioni
Tissue specificityi
Leaves, roots and stem.
Inductioni
By sulfate starvation.
Gene expression databases
ExpressionAtlasi | P92980, baseline and differential |
Genevisiblei | P92980, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 13577, 1 interactor |
IntActi | P92980, 2 interactors |
STRINGi | 3702.AT4G21990.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 337 – 458 | ThioredoxinPROSITE-ProRule annotationAdd BLAST | 122 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 70 – 319 | Reductase domainAdd BLAST | 250 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 8 – 54 | Ser-richAdd BLAST | 47 |
Domaini
The C-terminal domain may function as glutaredoxin and mediates the interaction of the enzyme with glutathione (GSH). Active in GSH-dependent reduction of hydroxyethyldisulfide, cystine, dehydroascorbate, insulin disulfides and ribonucleotide reductase (By similarity).By similarity
Sequence similaritiesi
Belongs to the APS reductase family.Curated
Keywords - Domaini
Redox-active center, Transit peptidePhylogenomic databases
eggNOGi | KOG0189, Eukaryota KOG0191, Eukaryota |
OrthoDBi | 834258at2759 |
PhylomeDBi | P92980 |
Family and domain databases
CDDi | cd01713, PAPS_reductase, 1 hit |
Gene3Di | 3.40.30.10, 1 hit 3.40.50.620, 1 hit |
InterProi | View protein in InterPro IPR002500, PAPS_reduct IPR014729, Rossmann-like_a/b/a_fold IPR004508, Thioredoxin-indep_APS_Rdtase IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF01507, PAPS_reduct, 1 hit PF00085, Thioredoxin, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
TIGRFAMsi | TIGR00424, APS_reduc, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketNote: A number of isoforms are produced. According to EST sequences.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: P92980-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MALAINVSSS SSSAISSSSF PSSDLKVTKI GSLRLLNRTN VSAASLSLSG
60 70 80 90 100
KRSSVKALNV QSITKESIVA SEVTEKLDVV EVEDFEELAK RLENASPLEI
110 120 130 140 150
MDKALEKFGN DIAIAFSGAE DVALIEYAHL TGRPYRVFSL DTGRLNPETY
160 170 180 190 200
RLFDTVEKHY GIRIEYMFPD AVEVQALVRN KGLFSFYEDG HQECCRIRKV
210 220 230 240 250
RPLRRALKGL RAWITGQRKD QSPGTRSEIP VVQVDPVFEG LDGGVGSLVK
260 270 280 290 300
WNPVANVEGN DVWNFLRTMD VPVNTLHAAG YVSIGCEPCT RAVLPGQHER
310 320 330 340 350
EGRWWWEDAK AKECGLHKGN IKENTNGNAT ANVNGTASVA DIFNSENVVN
360 370 380 390 400
LSRQGIENLM KLENRKEAWI VVLYAPWCPF CQAMEASFDE LADKLGGSGV
410 420 430 440 450
KVAKFRADGD QKDFAKKELQ LGSFPTILVF PKNSSRPIKY PSEKRDVDSL
TSFLNLVR
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF4JKD5 | F4JKD5_ARATH | APS reductase 3 | APR3 APS reductase 3, ATAPR3, PAPS REDUCTASE HOMOLOG 26, PRH-26, PRH26 | 340 | Annotation score: |
Sequence cautioni
The sequence AAC26978 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 28 | T → A in AAC26978 (PubMed:8917600).Curated | 1 | |
Sequence conflicti | 121 | D → Y in AAC26978 (PubMed:8917600).Curated | 1 | |
Sequence conflicti | 264 | N → D in BAH57002 (PubMed:19423640).Curated | 1 | |
Sequence conflicti | 338 | S → T in AAM65133 (Ref. 8) Curated | 1 | |
Sequence conflicti | 375 | A → R in AAC49562 (PubMed:8917599).Curated | 1 | |
Sequence conflicti | 436 | R → I in AAC49562 (PubMed:8917599).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U53865 mRNA Translation: AAC49562.1 U56922 mRNA Translation: AAC26978.1 Frameshift. AF016284 Genomic DNA Translation: AAC26981.1 AL022140 Genomic DNA Translation: CAA18102.1 AL161556 Genomic DNA Translation: CAB79154.1 CP002687 Genomic DNA Translation: AEE84539.1 AF428445 mRNA Translation: AAL16214.1 AY054175 mRNA Translation: AAL06836.1 AY062665 mRNA Translation: AAL32743.1 AY093319 mRNA Translation: AAM13318.1 AY103313 mRNA Translation: AAM65364.1 AK318887 mRNA Translation: BAH57002.1 AY087591 mRNA Translation: AAM65133.1 |
PIRi | T49106 |
RefSeqi | NP_193930.1, NM_118320.3 [P92980-1] |
Genome annotation databases
EnsemblPlantsi | AT4G21990.1; AT4G21990.1; AT4G21990 [P92980-1] |
GeneIDi | 828288 |
Gramenei | AT4G21990.1; AT4G21990.1; AT4G21990 [P92980-1] |
KEGGi | ath:AT4G21990 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U53865 mRNA Translation: AAC49562.1 U56922 mRNA Translation: AAC26978.1 Frameshift. AF016284 Genomic DNA Translation: AAC26981.1 AL022140 Genomic DNA Translation: CAA18102.1 AL161556 Genomic DNA Translation: CAB79154.1 CP002687 Genomic DNA Translation: AEE84539.1 AF428445 mRNA Translation: AAL16214.1 AY054175 mRNA Translation: AAL06836.1 AY062665 mRNA Translation: AAL32743.1 AY093319 mRNA Translation: AAM13318.1 AY103313 mRNA Translation: AAM65364.1 AK318887 mRNA Translation: BAH57002.1 AY087591 mRNA Translation: AAM65133.1 |
PIRi | T49106 |
RefSeqi | NP_193930.1, NM_118320.3 [P92980-1] |
3D structure databases
SMRi | P92980 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 13577, 1 interactor |
IntActi | P92980, 2 interactors |
STRINGi | 3702.AT4G21990.1 |
Proteomic databases
PaxDbi | P92980 |
PRIDEi | P92980 |
ProteomicsDBi | 244440 [P92980-1] |
Genome annotation databases
EnsemblPlantsi | AT4G21990.1; AT4G21990.1; AT4G21990 [P92980-1] |
GeneIDi | 828288 |
Gramenei | AT4G21990.1; AT4G21990.1; AT4G21990 [P92980-1] |
KEGGi | ath:AT4G21990 |
Organism-specific databases
Araporti | AT4G21990 |
TAIRi | locus:2120628, AT4G21990 |
Phylogenomic databases
eggNOGi | KOG0189, Eukaryota KOG0191, Eukaryota |
OrthoDBi | 834258at2759 |
PhylomeDBi | P92980 |
Enzyme and pathway databases
BioCyci | MetaCyc:AT4G21990-MONOMER |
Miscellaneous databases
PROi | PR:P92980 |
Gene expression databases
ExpressionAtlasi | P92980, baseline and differential |
Genevisiblei | P92980, AT |
Family and domain databases
CDDi | cd01713, PAPS_reductase, 1 hit |
Gene3Di | 3.40.30.10, 1 hit 3.40.50.620, 1 hit |
InterProi | View protein in InterPro IPR002500, PAPS_reduct IPR014729, Rossmann-like_a/b/a_fold IPR004508, Thioredoxin-indep_APS_Rdtase IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF01507, PAPS_reduct, 1 hit PF00085, Thioredoxin, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
TIGRFAMsi | TIGR00424, APS_reduc, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | APR3_ARATH | |
Accessioni | P92980Primary (citable) accession number: P92980 Secondary accession number(s): C0Z2S3 Q8LAV2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 19, 2002 |
Last sequence update: | October 19, 2002 | |
Last modified: | December 2, 2020 | |
This is version 166 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families