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UniProtKB - P92979 (APR1_ARATH)
Protein
5'-adenylylsulfate reductase 1, chloroplastic
Gene
APR1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Reduces sulfate for Cys biosynthesis. Substrate preference is adenosine-5'-phosphosulfate (APS) >> 3'-phosphoadenosine-5'-phosphosulfate (PAPS). Uses glutathione or DTT as source of protons.
Catalytic activityi
- EC:1.8.4.9
Cofactori
[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity
Activity regulationi
Stimulated by sodium sulfate > ammonium sulfate and is sensitive to inactivation by 5'AMP.
pH dependencei
Optimum pH is 8.5.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 385 | NucleophileBy similarity | 1 | |
Active sitei | 388 | NucleophileBy similarity | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- adenylyl-sulfate reductase (glutathione) activity Source: UniProtKB-EC
- adenylyl-sulfate reductase activity Source: TAIR
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- cysteine biosynthetic process Source: UniProtKB-KW
- sulfate assimilation Source: TAIR
- sulfate reduction Source: TAIR
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Amino-acid biosynthesis, Cysteine biosynthesis, Stress response |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
BRENDAi | 1.8.4.9, 399 |
Names & Taxonomyi
Protein namesi | Recommended name: 5'-adenylylsulfate reductase 1, chloroplastic (EC:1.8.4.9)Alternative name(s): 3'-phosphoadenosine-5'-phosphosulfate reductase homolog 19 Short name: PAPS reductase homolog 19 Short name: Prh-19 Adenosine 5'-phosphosulfate 5'-adenylylsulfate sulfotransferase 1 Short name: APS sulfotransferase 1 Thioredoxin-independent APS reductase 1 |
Gene namesi | Name:APR1 Synonyms:PRH19 Ordered Locus Names:At4g04610 ORF Names:F4H6.13 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G04610 |
TAIRi | locus:2125786, AT4G04610 |
Subcellular locationi
Chloroplast
- chloroplast Curated
Chloroplast
- chloroplast Source: TAIR
- chloroplast stroma Source: TAIR
Other locations
- plastid Source: TAIR
Keywords - Cellular componenti
Chloroplast, PlastidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 53 | ChloroplastSequence analysisAdd BLAST | 53 | |
ChainiPRO_0000023214 | 54 – 465 | 5'-adenylylsulfate reductase 1, chloroplasticAdd BLAST | 412 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 385 ↔ 388 | Redox-activePROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bondProteomic databases
PaxDbi | P92979 |
PRIDEi | P92979 |
ProteomicsDBi | 240602 |
Expressioni
Tissue specificityi
Leaves, roots and stem.
Inductioni
Gene expression databases
ExpressionAtlasi | P92979, baseline and differential |
Genevisiblei | P92979, AT |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P92979 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 344 – 465 | ThioredoxinPROSITE-ProRule annotationAdd BLAST | 122 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 73 – 327 | Reductase domainAdd BLAST | 255 |
Domaini
The C-terminal domain may function as glutaredoxin and mediates the interaction of the enzyme with glutathione (GSH). Active in GSH-dependent reduction of hydroxyethyldisulfide, cystine, dehydroascorbate, insulin disulfides and ribonucleotide reductase.
Sequence similaritiesi
Belongs to the APS reductase family.Curated
Keywords - Domaini
Redox-active center, Transit peptidePhylogenomic databases
eggNOGi | KOG0189, Eukaryota KOG0191, Eukaryota |
HOGENOMi | CLU_044089_4_0_1 |
OMAi | HAWITGQ |
OrthoDBi | 834258at2759 |
PhylomeDBi | P92979 |
Family and domain databases
CDDi | cd01713, PAPS_reductase, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
HAMAPi | MF_00063, CysH, 1 hit |
InterProi | View protein in InterPro IPR002500, PAPS_reduct IPR014729, Rossmann-like_a/b/a_fold IPR004508, Thioredoxin-indep_APS_Rdtase IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF01507, PAPS_reduct, 1 hit PF00085, Thioredoxin, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
TIGRFAMsi | TIGR00424, APS_reduc, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P92979-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAMSVNVSSS SSSGIINSRF GVSLEPKVSQ IGSLRLLDRV HVAPVSLNLS
60 70 80 90 100
GKRSSSVKPL NAEPKTKDSM IPLAATMVAE IAEEVEVVEI EDFEELAKKL
110 120 130 140 150
ENASPLEIMD KALEKYGNDI AIAFSGAEDV ALIEYAHLTG RPFRVFSLDT
160 170 180 190 200
GRLNPETYRF FDAVEKHYGI RIEYMFPDSV EVQGLVRSKG LFSFYEDGHQ
210 220 230 240 250
ECCRVRKVRP LRRALKGLKA WITGQRKDQS PGTRSEIPVV QVDPVFEGLD
260 270 280 290 300
GGVGSLVKWN PVANVEGNDV WNFLRTMDVP VNTLHAAGYI SIGCEPCTKA
310 320 330 340 350
VLPGQHEREG RWWWEDAKAK ECGLHKGNVK ENSDDAKVNG ESKSAVADIF
360 370 380 390 400
KSENLVTLSR QGIENLMKLE NRKEPWIVVL YAPWCPFCQA MEASYDELAD
410 420 430 440 450
KLAGSGIKVA KFRADGDQKE FAKQELQLGS FPTILVFPKN SSRPIKYPSE
460
KRDVESLTSF LNLVR
Sequence cautioni
The sequence AAC49573 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 67 | K → N in AAM65557 (Ref. 7) Curated | 1 | |
Sequence conflicti | 86 | E → D in AAC49573 (PubMed:8917600).Curated | 1 | |
Sequence conflicti | 249 – 253 | LDGGV → WMVEF in AAC49573 (PubMed:8917600).Curated | 5 | |
Sequence conflicti | 371 | N → F in AAC49561 (PubMed:8917599).Curated | 1 | |
Sequence conflicti | 392 | Missing in AAC49573 (PubMed:8917600).Curated | 1 | |
Sequence conflicti | 400 | D → A in AAC49561 (PubMed:8917599).Curated | 1 | |
Sequence conflicti | 407 | I → D in AAC49561 (PubMed:8917599).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U53864 mRNA Translation: AAC49561.1 U43412 mRNA Translation: AAC49573.1 Frameshift. AF016282 Genomic DNA Translation: AAC26979.1 AF074021 Genomic DNA Translation: AAD29775.1 AL161501 Genomic DNA Translation: CAB80826.1 CP002687 Genomic DNA Translation: AEE82402.1 AF424582 mRNA Translation: AAL11576.1 BT002612 mRNA Translation: AAO11528.1 AY088011 mRNA Translation: AAM65557.1 AK220828 mRNA Translation: BAD94133.1 |
PIRi | B85058 |
RefSeqi | NP_192370.1, NM_116699.3 |
Genome annotation databases
EnsemblPlantsi | AT4G04610.1; AT4G04610.1; AT4G04610 |
GeneIDi | 825793 |
Gramenei | AT4G04610.1; AT4G04610.1; AT4G04610 |
KEGGi | ath:AT4G04610 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U53864 mRNA Translation: AAC49561.1 U43412 mRNA Translation: AAC49573.1 Frameshift. AF016282 Genomic DNA Translation: AAC26979.1 AF074021 Genomic DNA Translation: AAD29775.1 AL161501 Genomic DNA Translation: CAB80826.1 CP002687 Genomic DNA Translation: AEE82402.1 AF424582 mRNA Translation: AAL11576.1 BT002612 mRNA Translation: AAO11528.1 AY088011 mRNA Translation: AAM65557.1 AK220828 mRNA Translation: BAD94133.1 |
PIRi | B85058 |
RefSeqi | NP_192370.1, NM_116699.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5YRY | X-ray | 2.70 | A | 353-461 | [»] | |
SMRi | P92979 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT4G04610.1 |
Proteomic databases
PaxDbi | P92979 |
PRIDEi | P92979 |
ProteomicsDBi | 240602 |
Genome annotation databases
EnsemblPlantsi | AT4G04610.1; AT4G04610.1; AT4G04610 |
GeneIDi | 825793 |
Gramenei | AT4G04610.1; AT4G04610.1; AT4G04610 |
KEGGi | ath:AT4G04610 |
Organism-specific databases
Araporti | AT4G04610 |
TAIRi | locus:2125786, AT4G04610 |
Phylogenomic databases
eggNOGi | KOG0189, Eukaryota KOG0191, Eukaryota |
HOGENOMi | CLU_044089_4_0_1 |
OMAi | HAWITGQ |
OrthoDBi | 834258at2759 |
PhylomeDBi | P92979 |
Enzyme and pathway databases
BRENDAi | 1.8.4.9, 399 |
Miscellaneous databases
PROi | PR:P92979 |
Gene expression databases
ExpressionAtlasi | P92979, baseline and differential |
Genevisiblei | P92979, AT |
Family and domain databases
CDDi | cd01713, PAPS_reductase, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
HAMAPi | MF_00063, CysH, 1 hit |
InterProi | View protein in InterPro IPR002500, PAPS_reduct IPR014729, Rossmann-like_a/b/a_fold IPR004508, Thioredoxin-indep_APS_Rdtase IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF01507, PAPS_reduct, 1 hit PF00085, Thioredoxin, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
TIGRFAMsi | TIGR00424, APS_reduc, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | APR1_ARATH | |
Accessioni | P92979Primary (citable) accession number: P92979 Secondary accession number(s): O48886 Q8LA60 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 19, 2002 |
Last sequence update: | October 19, 2002 | |
Last modified: | February 23, 2022 | |
This is version 167 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families