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Protein

Chloride channel protein CLC-a

Gene

CLC-A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Voltage-gated chloride channel that could play a role in the regulation of nitrate content.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChloride channel, Ion channel, Voltage-gated channel
Biological processIon transport, Transport
LigandChloride

Enzyme and pathway databases

ReactomeiR-ATH-2672351 Stimuli-sensing channels

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride channel protein CLC-a
Short name:
AtCLC-a
Alternative name(s):
CBS domain-containing protein CBSCLC5
Gene namesi
Name:CLC-A
Synonyms:CBSCLC5
Ordered Locus Names:At5g40890
ORF Names:MHK7.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G40890
TAIRilocus:2164466 AT5G40890

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei88 – 108Helical; Name=1Sequence analysisAdd BLAST21
Transmembranei131 – 151Helical; Name=2Sequence analysisAdd BLAST21
Transmembranei178 – 198Helical; Name=3Sequence analysisAdd BLAST21
Transmembranei206 – 226Helical; Name=4Sequence analysisAdd BLAST21
Transmembranei248 – 268Helical; Name=5Sequence analysisAdd BLAST21
Transmembranei278 – 298Helical; Name=6Sequence analysisAdd BLAST21
Transmembranei328 – 348Helical; Name=7Sequence analysisAdd BLAST21
Transmembranei371 – 391Helical; Name=8Sequence analysisAdd BLAST21
Transmembranei453 – 473Helical; Name=9Sequence analysisAdd BLAST21
Transmembranei478 – 498Helical; Name=10Sequence analysisAdd BLAST21
Transmembranei510 – 530Helical; Name=11Sequence analysisAdd BLAST21
Transmembranei531 – 551Helical; Name=12Sequence analysisAdd BLAST21
Transmembranei730 – 750Helical; Name=13Sequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Loss-of-function mutation clca-1 leads to an altered nitrate content and hypersensitivity to chlorate.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000944651 – 775Chloride channel protein CLC-aAdd BLAST775

Proteomic databases

PaxDbiP92941
PRIDEiP92941

PTM databases

iPTMnetiP92941

Expressioni

Tissue specificityi

Broadly expressed in the plant.

Inductioni

In shoots and roots by nitrate treatment.1 Publication

Gene expression databases

ExpressionAtlasiP92941 baseline and differential
GenevisibleiP92941 AT

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with PP2A5 (PubMed:27676158).By similarity1 Publication

Protein-protein interaction databases

BioGridi19341, 5 interactors
IntActiP92941, 5 interactors
STRINGi3702.AT5G40890.1

Structurei

3D structure databases

ProteinModelPortaliP92941
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini595 – 658CBS 1PROSITE-ProRule annotationAdd BLAST64
Domaini703 – 768CBS 2PROSITE-ProRule annotationAdd BLAST66

Sequence similaritiesi

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0474 Eukaryota
COG0038 LUCA
HOGENOMiHOG000231081
InParanoidiP92941
KOiK05016
OMAiSEMQLYV
OrthoDBiEOG093602TR
PhylomeDBiP92941

Family and domain databases

InterProiView protein in InterPro
IPR000644 CBS_dom
IPR014743 Cl-channel_core
IPR001807 Cl-channel_volt-gated
IPR002251 Cl_channel_pln
PfamiView protein in Pfam
PF00571 CBS, 2 hits
PF00654 Voltage_CLC, 1 hit
PRINTSiPR00762 CLCHANNEL
PR01120 CLCHANNELPLT
SMARTiView protein in SMART
SM00116 CBS, 2 hits
SUPFAMiSSF81340 SSF81340, 2 hits
PROSITEiView protein in PROSITE
PS51371 CBS, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket
Note: A number of isoforms are produced. According to EST sequences.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: P92941-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDEDGNLQIS NSNYNGEEEG EDPENNTLNQ PLLKRHRTLS STPLALVGAK
60 70 80 90 100
VSHIESLDYE INENDLFKHD WRSRSKAQVF QYIFLKWTLA CLVGLFTGLI
110 120 130 140 150
ATLINLAVEN IAGYKLLAVG YYIAQDRFWT GLMVFTGANL GLTLVATVLV
160 170 180 190 200
VYFAPTAAGP GIPEIKAYLN GIDTPNMFGF TTMMVKIVGS IGAVAAGLDL
210 220 230 240 250
GKEGPLVHIG SCIASLLGQG GPDNHRIKWR WLRYFNNDRD RRDLITCGSA
260 270 280 290 300
SGVCAAFRSP VGGVLFALEE VATWWRSALL WRTFFSTAVV VVVLRAFIEI
310 320 330 340 350
CNSGKCGLFG SGGLIMFDVS HVEVRYHAAD IIPVTLIGVF GGILGSLYNH
360 370 380 390 400
LLHKVLRLYN LINQKGKIHK VLLSLGVSLF TSVCLFGLPF LAECKPCDPS
410 420 430 440 450
IDEICPTNGR SGNFKQFNCP NGYYNDLSTL LLTTNDDAVR NIFSSNTPNE
460 470 480 490 500
FGMVSLWIFF GLYCILGLIT FGIATPSGLF LPIILMGSAY GRMLGTAMGS
510 520 530 540 550
YTNIDQGLYA VLGAASLMAG SMRMTVSLCV IFLELTNNLL LLPITMFVLL
560 570 580 590 600
IAKTVGDSFN LSIYEIILHL KGLPFLEANP EPWMRNLTVG ELNDAKPPVV
610 620 630 640 650
TLNGVEKVAN IVDVLRNTTH NAFPVLDGAD QNTGTELHGL ILRAHLVKVL
660 670 680 690 700
KKRWFLNEKR RTEEWEVREK FTPVELAERE DNFDDVAITS SEMQLYVDLH
710 720 730 740 750
PLTNTTPYTV VQSMSVAKAL VLFRSVGLRH LLVVPKIQAS GMSPVIGILT
760 770
RQDLRAYNIL QAFPHLDKHK SGKAR
Length:775
Mass (Da):85,406
Last modified:February 2, 2004 - v2
Checksum:iA352FB24583E714E
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F4KIT3F4KIT3_ARATH
Chloride channel A
CLC-A ATCLC-A, ATCLCA, CHLORIDE CHANNEL A, chloride channel A, CHLORIDE CHANNEL-A CLC-A ATCLC-A, ATCLCA, CHLORIDE CHANNEL A, chloride channel A, CHLORIDE CHANNEL-A, CLCA, At5g40890
643Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti330D → H in CAA96057 (PubMed:8969232).Curated1
Sequence conflicti506Q → L in AAL24139 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71445 mRNA Translation: CAA96057.1
AF044313 mRNA Translation: AAC05742.1
AB011477 Genomic DNA Translation: BAB11351.1
CP002688 Genomic DNA Translation: AED94612.1
AY059791 mRNA Translation: AAL24139.1
AY150506 mRNA Translation: AAN13022.1
PIRiT52107
RefSeqiNP_198905.1, NM_123454.3 [P92941-1]
UniGeneiAt.25457

Genome annotation databases

EnsemblPlantsiAT5G40890.1; AT5G40890.1; AT5G40890 [P92941-1]
GeneIDi834090
GrameneiAT5G40890.1; AT5G40890.1; AT5G40890 [P92941-1]
KEGGiath:AT5G40890

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71445 mRNA Translation: CAA96057.1
AF044313 mRNA Translation: AAC05742.1
AB011477 Genomic DNA Translation: BAB11351.1
CP002688 Genomic DNA Translation: AED94612.1
AY059791 mRNA Translation: AAL24139.1
AY150506 mRNA Translation: AAN13022.1
PIRiT52107
RefSeqiNP_198905.1, NM_123454.3 [P92941-1]
UniGeneiAt.25457

3D structure databases

ProteinModelPortaliP92941
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19341, 5 interactors
IntActiP92941, 5 interactors
STRINGi3702.AT5G40890.1

PTM databases

iPTMnetiP92941

Proteomic databases

PaxDbiP92941
PRIDEiP92941

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G40890.1; AT5G40890.1; AT5G40890 [P92941-1]
GeneIDi834090
GrameneiAT5G40890.1; AT5G40890.1; AT5G40890 [P92941-1]
KEGGiath:AT5G40890

Organism-specific databases

AraportiAT5G40890
TAIRilocus:2164466 AT5G40890

Phylogenomic databases

eggNOGiKOG0474 Eukaryota
COG0038 LUCA
HOGENOMiHOG000231081
InParanoidiP92941
KOiK05016
OMAiSEMQLYV
OrthoDBiEOG093602TR
PhylomeDBiP92941

Enzyme and pathway databases

ReactomeiR-ATH-2672351 Stimuli-sensing channels

Miscellaneous databases

PROiPR:P92941

Gene expression databases

ExpressionAtlasiP92941 baseline and differential
GenevisibleiP92941 AT

Family and domain databases

InterProiView protein in InterPro
IPR000644 CBS_dom
IPR014743 Cl-channel_core
IPR001807 Cl-channel_volt-gated
IPR002251 Cl_channel_pln
PfamiView protein in Pfam
PF00571 CBS, 2 hits
PF00654 Voltage_CLC, 1 hit
PRINTSiPR00762 CLCHANNEL
PR01120 CLCHANNELPLT
SMARTiView protein in SMART
SM00116 CBS, 2 hits
SUPFAMiSSF81340 SSF81340, 2 hits
PROSITEiView protein in PROSITE
PS51371 CBS, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiCLCA_ARATH
AccessioniPrimary (citable) accession number: P92941
Secondary accession number(s): O64990, Q93YS0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: February 2, 2004
Last modified: April 25, 2018
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
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