ECA1

Functionⁱ

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the endoplasmic reticulum lumen. Also regulate manganese ion homeostasis by pumping it into endomembrane compartments. Can also transport zinc.

Catalytic activityⁱ

ATP + H₂O + Ca²⁺(Side 1) = ADP + phosphate + Ca²⁺(Side 2).

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	336	Calcium 2; via carbonyl oxygenBy similarity	1
Metal bindingⁱ	337	Calcium 2; via carbonyl oxygenBy similarity	1
Metal bindingⁱ	339	Calcium 2; via carbonyl oxygenBy similarity	1
Metal bindingⁱ	341	Calcium 2By similarity	1
Active siteⁱ	383	4-aspartylphosphate intermediateBy similarity	1
Metal bindingⁱ	731	MagnesiumBy similarity	1
Metal bindingⁱ	735	MagnesiumBy similarity	1
Metal bindingⁱ	797	Calcium 1By similarity	1
Metal bindingⁱ	800	Calcium 1By similarity	1
Metal bindingⁱ	825	Calcium 2By similarity	1
Metal bindingⁱ	828	Calcium 1By similarity	1
Metal bindingⁱ	829	Calcium 1By similarity	1
Metal bindingⁱ	829	Calcium 2By similarity	1
Metal bindingⁱ	961	Calcium 1By similarity	1

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
calcium-transporting ATPase activity
Source: TAIR
Inferred from direct assayⁱ
- 9238019
metal ion binding Source: UniProtKB-KW
proton-exporting ATPase activity, phosphorylative mechanism
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase
Biological process	Calcium transport, Ion transport, Transport
Ligand	ATP-binding, Calcium, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	ARA:AT1G07810-MONOMER MetaCyc:MONOMER-14600
BRENDAⁱ	3.6.3.8 399

Protein family/group databases

TCDBⁱ	3.A.3.2.13 the p-type atpase (p-atpase) superfamily

TCDBⁱ

3.A.3.2.13 the p-type atpase (p-atpase) superfamily

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Calcium-transporting ATPase 1, endoplasmic reticulum-type (EC:3.6.3.8)
Gene namesⁱ	Name:ECA1 Synonyms:ACA3 Ordered Locus Names:At1g07810 ORF Names:F24B9.9
Organismⁱ	Arabidopsis thaliana (Mouse-ear cress)
Taxonomic identifierⁱ	3702 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Mesangiospermae › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis
Proteomesⁱ	UP000006548 Componentⁱ: Chromosome 1

Organism-specific databases

Arabidopsis Information Portal More...Araportⁱ	AT1G07810
TAIRⁱ	locus:2026580 AT1G07810

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 70	CytoplasmicSequence analysisAdd BLAST	70
Transmembraneⁱ	71 – 91	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	92 – 115	LumenalSequence analysisAdd BLAST	24
Transmembraneⁱ	116 – 135	HelicalSequence analysisAdd BLAST	20
Topological domainⁱ	136 – 278	CytoplasmicSequence analysisAdd BLAST	143
Transmembraneⁱ	279 – 298	HelicalSequence analysisAdd BLAST	20
Topological domainⁱ	299 – 327	LumenalSequence analysisAdd BLAST	29
Transmembraneⁱ	328 – 345	HelicalSequence analysisAdd BLAST	18
Topological domainⁱ	346 – 786	CytoplasmicSequence analysisAdd BLAST	441
Transmembraneⁱ	787 – 806	HelicalSequence analysisAdd BLAST	20
Topological domainⁱ	807 – 816	LumenalSequence analysis	10
Transmembraneⁱ	817 – 837	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	838 – 857	CytoplasmicSequence analysisAdd BLAST	20
Transmembraneⁱ	858 – 880	HelicalSequence analysisAdd BLAST	23
Topological domainⁱ	881 – 950	LumenalSequence analysisAdd BLAST	70
Transmembraneⁱ	951 – 970	HelicalSequence analysisAdd BLAST	20
Topological domainⁱ	971 – 983	CytoplasmicSequence analysisAdd BLAST	13
Transmembraneⁱ	984 – 1002	HelicalSequence analysisAdd BLAST	19
Topological domainⁱ	1003 – 1017	LumenalSequence analysisAdd BLAST	15
Transmembraneⁱ	1018 – 1038	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	1039 – 1061	CytoplasmicSequence analysisAdd BLAST	23

Keywords - Cellular componentⁱ

Endoplasmic reticulum, Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000046405	1 – 1061	Calcium-transporting ATPase 1, endoplasmic reticulum-typeAdd BLAST		1061

Proteomic databases

PaxDbⁱ	P92939
PRIDEⁱ	P92939

PTM databases

iPTMnetⁱ	P92939

iPTMnetⁱ

P92939

Expressionⁱ

Gene expression databases

ExpressionAtlasⁱ	P92939 baseline and differential
Genevisibleⁱ	P92939 AT

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	22537, 3 interactors
STRINGⁱ	3702.AT1G07810.1

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P92939
SMRⁱ	P92939
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. [View classification]Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG0202 Eukaryota COG0474 LUCA
HOGENOMⁱ	HOG000265621
InParanoidⁱ	P92939
KEGG Orthology (KO) More...KOⁱ	K01537
OMAⁱ	QCSVFES
Database of Orthologous Groups More...OrthoDBⁱ	EOG093600UE
PhylomeDBⁱ	P92939

Family and domain databases

Gene3Dⁱ	3.40.1110.10, 1 hit 3.40.50.1000, 1 hit
InterProⁱ	View protein in InterPro IPR006068 ATPase_P-typ_cation-transptr_C IPR004014 ATPase_P-typ_cation-transptr_N IPR023299 ATPase_P-typ_cyto_dom_N IPR018303 ATPase_P-typ_P_site IPR023298 ATPase_P-typ_TM_dom_sf IPR008250 ATPase_P-typ_transduc_dom_A_sf IPR036412 HAD-like_sf IPR023214 HAD_sf IPR001757 P_typ_ATPase
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00689 Cation_ATPase_C, 1 hit PF00690 Cation_ATPase_N, 1 hit
SMARTⁱ	View protein in SMART SM00831 Cation_ATPase_N, 1 hit
SUPFAMⁱ	SSF56784 SSF56784, 1 hit SSF81653 SSF81653, 1 hit SSF81660 SSF81660, 1 hit SSF81665 SSF81665, 1 hit
TIGRFAMsⁱ	TIGR01494 ATPase_P-type, 3 hits
PROSITEⁱ	View protein in PROSITE PS00154 ATPASE_E1_E2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P92939-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MGKGSEDLVK KESLNSTPVN SDTFPAWAKD VAECEEHFVV SREKGLSSDE 
        60         70         80         90        100
VLKRHQIYGL NELEKPEGTS IFKLILEQFN DTLVRILLAA AVISFVLAFF 
       110        120        130        140        150
DGDEGGEMGI TAFVEPLVIF LILIVNAIVG IWQETNAEKA LEALKEIQSQ 
       160        170        180        190        200
QATVMRDGTK VSSLPAKELV PGDIVELRVG DKVPADMRVV ALISSTLRVE 
       210        220        230        240        250
QGSLTGESEA VSKTTKHVDE NADIQGKKCM VFAGTTVVNG NCICLVTDTG 
       260        270        280        290        300
MNTEIGRVHS QIQEAAQHEE DTPLKKKLNE FGEVLTMIIG LICALVWLIN 
       310        320        330        340        350
VKYFLSWEYV DGWPRNFKFS FEKCTYYFEI AVALAVAAIP EGLPAVITTC 
       360        370        380        390        400
LALGTRKMAQ KNALVRKLPS VETLGCTTVI CSDKTGTLTT NQMAVSKLVA 
       410        420        430        440        450
MGSRIGTLRS FNVEGTSFDP RDGKIEDWPM GRMDANLQMI AKIAAICNDA 
       460        470        480        490        500
NVEQSDQQFV SRGMPTEAAL KVLVEKMGFP EGLNEASSDG DVLRCCRLWS 
       510        520        530        540        550
ELEQRIATLE FDRDRKSMGV MVDSSSGNKL LLVKGAVENV LERSTHIQLL 
       560        570        580        590        600
DGSKRELDQY SRDLILQSLR DMSLSALRCL GFAYSDVPSD FATYDGSEDH 
       610        620        630        640        650
PAHQQLLNPS NYSSIESNLI FVGFVGLRDP PRKEVRQAIA DCRTAGIRVM 
       660        670        680        690        700
VITGDNKSTA EAICREIGVF EADEDISSRS LTGIEFMDVQ DQKNHLRQTG 
       710        720        730        740        750
GLLFSRAEPK HKQEIVRLLK EDGEVVAMTG DGVNDAPALK LADIGVAMGI 
       760        770        780        790        800
SGTEVAKEAS DMVLADDNFS TIVAAVGEGR SIYNNMKAFI RYMISSNIGE 
       810        820        830        840        850
VASIFLTAAL GIPEGMIPVQ LLWVNLVTDG PPATALGFNP PDKDIMKKPP 
       860        870        880        890        900
RRSDDSLITA WILFRYMVIG LYVGVATVGV FIIWYTHSSF MGIDLSQDGH 
       910        920        930        940        950
SLVSYSQLAH WGQCSSWEGF KVSPFTAGSQ TFSFDSNPCD YFQQGKIKAS 
       960        970        980        990       1000
TLSLSVLVAI EMFNSLNALS EDGSLVTMPP WVNPWLLLAM AVSFGLHFVI 
      1010       1020       1030       1040       1050
LYVPFLAQVF GIVPLSLNEW LLVLAVSLPV ILIDEVLKFV GRCTSGYRYS 
      1060 
PRTLSTKQKE E

Length:1,061

Mass (Da):116,366

Last modified:January 11, 2001 - v2

Checksum:ⁱ4281842216E1340F

GO

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱ	684	I → K in strain: cv. Landsberg erecta.		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U96455 mRNA Translation: AAC68819.1 U93845 mRNA Translation: AAB52420.1 AC007583 Genomic DNA Translation: AAF75073.1 CP002684 Genomic DNA Translation: AEE28186.1 AF116533 Genomic DNA Translation: AAF36087.1 Y09817 mRNA Translation: CAA70946.1
PIRⁱ	E86213
NCBI Reference Sequences More...RefSeqⁱ	NP_172259.1, NM_100655.4
UniGeneⁱ	At.25348 At.26214

Genome annotation databases

EnsemblPlantsⁱ	AT1G07810.1; AT1G07810.1; AT1G07810
GeneIDⁱ	837296
Grameneⁱ	AT1G07810.1; AT1G07810.1; AT1G07810
KEGGⁱ	ath:AT1G07810

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P92939	Calcium-transporting ATPase 4, endoplasmic reticulum-type		ARATH		1061	UniRef90_P92939
Uncharacterized protein		BRARP		1194
Uncharacterized protein		Brassica oleracea var. oleracea		1089
Calcium-transporting ATPase 4, endoplasmic reticulum-type		NOCCA		1063
Uncharacterized protein		EUTSA		1062
+29

Protein	Similar proteins		Species	Score	Length	Source
P92939	Calcium-transporting ATPase 4, endoplasmic reticulum-type		ARATH		1061	UniRef50_P92939
Calcium-transporting ATPase, endoplasmic reticulum-type		SOLLC		1048
Uncharacterized protein		BRARP		1194
Uncharacterized protein		Brassica oleracea var. oleracea		1089
Calcium-transporting ATPase 4, endoplasmic reticulum-type		NOCCA		1063
+394

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U96455 mRNA Translation: AAC68819.1 U93845 mRNA Translation: AAB52420.1 AC007583 Genomic DNA Translation: AAF75073.1 CP002684 Genomic DNA Translation: AEE28186.1 AF116533 Genomic DNA Translation: AAF36087.1 Y09817 mRNA Translation: CAA70946.1
PIRⁱ	E86213
RefSeqⁱ	NP_172259.1, NM_100655.4
UniGeneⁱ	At.25348 At.26214

3D structure databases

ProteinModelPortalⁱ	P92939
SMRⁱ	P92939
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	22537, 3 interactors
STRINGⁱ	3702.AT1G07810.1

Protein family/group databases

TCDBⁱ	3.A.3.2.13 the p-type atpase (p-atpase) superfamily

TCDBⁱ

3.A.3.2.13 the p-type atpase (p-atpase) superfamily

PTM databases

iPTMnetⁱ	P92939

iPTMnetⁱ

P92939

Proteomic databases

PaxDbⁱ	P92939
PRIDEⁱ	P92939

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblPlantsⁱ	AT1G07810.1; AT1G07810.1; AT1G07810
GeneIDⁱ	837296
Grameneⁱ	AT1G07810.1; AT1G07810.1; AT1G07810
KEGGⁱ	ath:AT1G07810

Organism-specific databases

Araportⁱ	AT1G07810
TAIRⁱ	locus:2026580 AT1G07810

Phylogenomic databases

eggNOGⁱ	KOG0202 Eukaryota COG0474 LUCA
HOGENOMⁱ	HOG000265621
InParanoidⁱ	P92939
KOⁱ	K01537
OMAⁱ	QCSVFES
OrthoDBⁱ	EOG093600UE
PhylomeDBⁱ	P92939

Enzyme and pathway databases

BioCycⁱ	ARA:AT1G07810-MONOMER MetaCyc:MONOMER-14600
BRENDAⁱ	3.6.3.8 399

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P92939

PROⁱ

PR:P92939

Gene expression databases

ExpressionAtlasⁱ	P92939 baseline and differential
Genevisibleⁱ	P92939 AT

Family and domain databases

Gene3Dⁱ	3.40.1110.10, 1 hit 3.40.50.1000, 1 hit
InterProⁱ	View protein in InterPro IPR006068 ATPase_P-typ_cation-transptr_C IPR004014 ATPase_P-typ_cation-transptr_N IPR023299 ATPase_P-typ_cyto_dom_N IPR018303 ATPase_P-typ_P_site IPR023298 ATPase_P-typ_TM_dom_sf IPR008250 ATPase_P-typ_transduc_dom_A_sf IPR036412 HAD-like_sf IPR023214 HAD_sf IPR001757 P_typ_ATPase
Pfamⁱ	View protein in Pfam PF00689 Cation_ATPase_C, 1 hit PF00690 Cation_ATPase_N, 1 hit
SMARTⁱ	View protein in SMART SM00831 Cation_ATPase_N, 1 hit
SUPFAMⁱ	SSF56784 SSF56784, 1 hit SSF81653 SSF81653, 1 hit SSF81660 SSF81660, 1 hit SSF81665 SSF81665, 1 hit
TIGRFAMsⁱ	TIGR01494 ATPase_P-type, 3 hits
PROSITEⁱ	View protein in PROSITE PS00154 ATPASE_E1_E2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ECA1_ARATH
Accessionⁱ	P92939Primary (citable) accession number: P92939 Secondary accession number(s): O04987
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
	Last sequence update:	January 11, 2001
	Last modified:	November 7, 2018
	This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Plant Protein Annotation Program

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UniProtKB - P92939 (ECA1_ARATH)

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Calcium-transporting ATPase 1, endoplasmic reticulum-type

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Endoplasmic reticulum

Endoplasmic reticulum

Plasma Membrane

Vacuole

Topology

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Natural variant

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ