UniProtKB - P91641 (MED20_DROME)
Mediator of RNA polymerase II transcription subunit 20
MED20
Functioni
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for activated transcription of the MtnA gene.
3 PublicationsGO - Molecular functioni
- transcription coregulator activity Source: InterPro
GO - Biological processi
- positive regulation of transcription, DNA-templated Source: GOC
- regulation of transcription by RNA polymerase II Source: FlyBase
Keywordsi
Molecular function | Activator |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Mediator of RNA polymerase II transcription subunit 20Alternative name(s): Mediator complex subunit 20 Protein trf-proximal dMED20 dTrap26 dTrfp |
Gene namesi | Name:MED20 Synonyms:Tmr, Trfp ORF Names:CG18780 |
Organismi | Drosophila melanogaster (Fruit fly)Imported |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0013531, MED20 |
VEuPathDBi | VectorBase:FBgn0013531 |
Subcellular locationi
Nucleus
- Nucleus Curated
Nucleus
- mediator complex Source: FlyBase
- nucleus Source: FlyBase
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000096373 | 1 – 252 | Mediator of RNA polymerase II transcription subunit 20Add BLAST | 252 |
Proteomic databases
PaxDbi | P91641 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | FBgn0013531, Expressed in embryo and 33 other tissues |
ExpressionAtlasi | P91641, baseline and differential |
Genevisiblei | P91641, DM |
Interactioni
Subunit structurei
Component of the Mediator complex, which includes at least CDK8, MED4, MED6, MED11, MED14, MED17, MED18, MED20, MED21, MED22, MED27, MED28, MED30 and MED31.
2 PublicationsBinary interactionsi
Protein-protein interaction databases
BioGRIDi | 68741, 34 interactors |
DIPi | DIP-21881N |
IntActi | P91641, 4 interactors |
STRINGi | 7227.FBpp0079166 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG4309, Eukaryota |
GeneTreei | ENSGT00390000002060 |
HOGENOMi | CLU_080044_1_0_1 |
InParanoidi | P91641 |
OMAi | NDIYEPM |
OrthoDBi | 1112080at2759 |
PhylomeDBi | P91641 |
Family and domain databases
InterProi | View protein in InterPro IPR013921, Mediator_Med20 |
PANTHERi | PTHR12465, PTHR12465, 1 hit |
Pfami | View protein in Pfam PF08612, Med20, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MGVTILQPYP LPEGKSGAHI IDQLSKRLLA LGATHAGQFL VDCETFISTP
60 70 80 90 100
QPHNGAPGRA VHVLHNSEYP ASTFSIIDNG TGKQVAIVAD NIFDLLMLKM
110 120 130 140 150
TNTFTSKKQT KIESRGARFE YGDFVIKLGS VTMMEHFKGI LIEIEYKSCV
160 170 180 190 200
ILAYCWEMIR EMLQGFLGIA VNKDFPSYFA PQTIMTAMGQ QQLHAKHNDI
210 220 230 240 250
FEPMDTVKQY LEQFTNYRKH VTLMGGMGSG PGSQQVGPNV HMSPAVAGLH
RS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketM9PCC3 | M9PCC3_DROME | Mediator of RNA polymerase II trans... | MED20 CG18267, Dmel\CG18780, dTrap26, dTrfp, l(2)28DE | 268 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 220 | H → Q in CAA71871 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y10975 mRNA Translation: CAA71871.1 AE014134 Genomic DNA Translation: AAF52599.2 BT003473 mRNA Translation: AAO39476.1 |
RefSeqi | NP_524663.2, NM_079924.3 |
Genome annotation databases
EnsemblMetazoai | FBtr0079544; FBpp0079166; FBgn0013531 |
GeneIDi | 43952 |
KEGGi | dme:Dmel_CG18780 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y10975 mRNA Translation: CAA71871.1 AE014134 Genomic DNA Translation: AAF52599.2 BT003473 mRNA Translation: AAO39476.1 |
RefSeqi | NP_524663.2, NM_079924.3 |
3D structure databases
AlphaFoldDBi | P91641 |
SMRi | P91641 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 68741, 34 interactors |
DIPi | DIP-21881N |
IntActi | P91641, 4 interactors |
STRINGi | 7227.FBpp0079166 |
Proteomic databases
PaxDbi | P91641 |
Protocols and materials databases
DNASUi | 43952 |
Genome annotation databases
EnsemblMetazoai | FBtr0079544; FBpp0079166; FBgn0013531 |
GeneIDi | 43952 |
KEGGi | dme:Dmel_CG18780 |
Organism-specific databases
CTDi | 9477 |
FlyBasei | FBgn0013531, MED20 |
VEuPathDBi | VectorBase:FBgn0013531 |
Phylogenomic databases
eggNOGi | KOG4309, Eukaryota |
GeneTreei | ENSGT00390000002060 |
HOGENOMi | CLU_080044_1_0_1 |
InParanoidi | P91641 |
OMAi | NDIYEPM |
OrthoDBi | 1112080at2759 |
PhylomeDBi | P91641 |
Miscellaneous databases
BioGRID-ORCSi | 43952, 0 hits in 1 CRISPR screen |
GenomeRNAii | 43952 |
PROi | PR:P91641 |
Gene expression databases
Bgeei | FBgn0013531, Expressed in embryo and 33 other tissues |
ExpressionAtlasi | P91641, baseline and differential |
Genevisiblei | P91641, DM |
Family and domain databases
InterProi | View protein in InterPro IPR013921, Mediator_Med20 |
PANTHERi | PTHR12465, PTHR12465, 1 hit |
Pfami | View protein in Pfam PF08612, Med20, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MED20_DROME | |
Accessioni | P91641Primary (citable) accession number: P91641 Secondary accession number(s): Q9VLT4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 28, 2003 |
Last sequence update: | March 28, 2003 | |
Last modified: | May 25, 2022 | |
This is version 158 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families