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UniProtKB - P90245 (POL1_BAMMN)
Protein
Genome polyprotein 1
Gene
N/A
Organism
Barley mild mosaic virus (strain Na1) (BaMMV)
Status
Functioni
Has helicase activity. It may be involved in replication (By similarity).
By similarityBoth 6K peptides are indispensable for virus replication.
By similarityHas proteolytic activity.
By similarityCatalytic activityi
- EC:2.7.7.48PROSITE-ProRule annotation
- Hydrolyzes glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or Gly) for the enzyme from tobacco etch virus. The natural substrate is the viral polyprotein, but other proteins and oligopeptides containing the appropriate consensus sequence are also cleaved. EC:3.4.22.44
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 1302 | For nuclear inclusion protein A activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1338 | For nuclear inclusion protein A activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1405 | For nuclear inclusion protein A activityPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 487 – 494 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- cysteine-type peptidase activity Source: InterPro
- helicase activity Source: UniProtKB-KW
- hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides Source: InterPro
- RNA binding Source: InterPro
- RNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW
- structural molecule activity Source: InterPro
GO - Biological processi
- RNA-protein covalent cross-linking Source: UniProtKB-KW
- transcription, DNA-templated Source: InterPro
- viral RNA genome replication Source: InterPro
Keywordsi
Molecular function | Helicase, Hydrolase, Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase |
Biological process | Viral RNA replication |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Genome polyprotein 1Cleaved into the following 8 chains: Alternative name(s): VPg Alternative name(s): NIa-pro Alternative name(s): RNA-directed RNA polymerase |
Organismi | Barley mild mosaic virus (strain Na1) (BaMMV) |
Taxonomic identifieri | 103900 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Pisuviricota › Stelpaviricetes › Patatavirales › Potyviridae › Bymovirus › |
Virus hosti | Hordeum vulgare (Barley) [TaxID: 4513] |
Proteomesi |
|
Subcellular locationi
- Virion Curated
Keywords - Cellular componenti
Capsid protein, Helical capsid protein, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000040530 | 1 – 292 | Protein P3Sequence analysisAdd BLAST | 292 | |
ChainiPRO_0000040531 | 293 – 359 | 6 kDa protein 1Sequence analysisAdd BLAST | 67 | |
ChainiPRO_0000040532 | 360 – 1017 | Cytoplasmic inclusion proteinSequence analysisAdd BLAST | 658 | |
ChainiPRO_0000040533 | 1018 – 1081 | 6 kDa protein 2Sequence analysisAdd BLAST | 64 | |
ChainiPRO_0000040534 | 1082 – 1256 | Viral genome-linked proteinSequence analysisAdd BLAST | 175 | |
ChainiPRO_0000040535 | 1257 – 1496 | Nuclear inclusion protein ASequence analysisAdd BLAST | 240 | |
ChainiPRO_0000040536 | 1497 – 2007 | Nuclear inclusion protein BSequence analysisAdd BLAST | 511 | |
ChainiPRO_0000040537 | 2008 – 2258 | Coat proteinSequence analysisAdd BLAST | 251 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1141 | O-(5'-phospho-RNA)-tyrosineBy similarity | 1 |
Post-translational modificationi
VPg is uridylylated by the polymerase and is covalently attached to the 5'-end of the genomic RNA. This uridylylated form acts as a nucleotide-peptide primer for the polymerase (By similarity).By similarity
The viral RNA1 of bymoviruses is expressed as a single polyprotein which undergoes post-translational proteolytic processing by the main proteinase NIa-pro resulting in the production of at least eight individual proteins.Curated
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 292 – 293 | CleavageSequence analysis | 2 | |
Sitei | 359 – 360 | CleavageSequence analysis | 2 | |
Sitei | 1017 – 1018 | CleavageSequence analysis | 2 | |
Sitei | 1081 – 1082 | CleavageSequence analysis | 2 | |
Sitei | 1256 – 1257 | CleavageSequence analysis | 2 | |
Sitei | 1496 – 1497 | CleavageSequence analysis | 2 | |
Sitei | 2007 – 2008 | CleavageSequence analysis | 2 |
Keywords - PTMi
Covalent protein-RNA linkage, PhosphoproteinProteomic databases
PRIDEi | P90245 |
Structurei
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 439 – 597 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 159 | |
Domaini | 612 – 778 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 167 | |
Domaini | 1257 – 1476 | Peptidase C4PROSITE-ProRule annotationAdd BLAST | 220 | |
Domaini | 1745 – 1869 | RdRp catalyticPROSITE-ProRule annotationAdd BLAST | 125 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2027 – 2047 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 2233 – 2258 | DisorderedSequence analysisAdd BLAST | 26 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 2240 – 2258 | Basic and acidic residuesSequence analysisAdd BLAST | 19 |
Sequence similaritiesi
Belongs to the bymoviruses polyprotein 1 family.Curated
Family and domain databases
Gene3Di | 2.40.10.10, 1 hit 3.30.70.270, 1 hit 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR043502, DNA/RNA_pol_sf IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001592, Poty_coat IPR001730, Potyv_NIa-pro_dom IPR013648, PP_Potyviridae IPR043128, Rev_trsase/Diguanyl_cyclase IPR001205, RNA-dir_pol_C IPR007094, RNA-dir_pol_PSvirus |
Pfami | View protein in Pfam PF00271, Helicase_C, 1 hit PF00863, Peptidase_C4, 1 hit PF00767, Poty_coat, 1 hit PF08440, Poty_PP, 1 hit PF00680, RdRP_1, 1 hit |
PRINTSi | PR00966, NIAPOTYPTASE |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF52540, SSF52540, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51436, POTYVIRUS_NIA_PRO, 1 hit PS50507, RDRP_SSRNA_POS, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P90245-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEEFIPEVFY QNQVQSLKKI LKSWKRDTSI YAYLAREQEV LAFLVLSPAH
60 70 80 90 100
IAKLNKLLTE ESARCALLAQ NCETIEALAV VRQALQGVTL HFGDNGMEKG
110 120 130 140 150
WLHMMKALDA CLDESFSENA AALKKSIQAV GHKLIAAKNR IESCERSVNH
160 170 180 190 200
LTTFQFAREY GLSTTYFEKL SNFGGHIRSF VWSSDSGEIF PKPKRSSRTK
210 220 230 240 250
RISTFTSYYW GEFWCNCIWL LCSLWSPARW CFNSVVFIWS LCGVLNLSMV
260 270 280 290 300
VLQFTLKRHF GRYYFRYVMS GVLAICAVCC VHLKNRKGPI LQASQKDKRF
310 320 330 340 350
IGILAFCITV IYMFDVDLAD SLSNNLHKIS RLVNLFLDDN RGFATPALDN
360 370 380 390 400
LTDFTTILQS GTSSDDLKIV QDTLAVVLQV DDEDATQDDA IYDSDGLQTF
410 420 430 440 450
KQWVSHNQLA GMQLARPLQY PCSTTYGLTA DNVAELATSM AQEAKQWSQV
460 470 480 490 500
VGHTGSGKST RLPTAYANCL KGLAGRKKNV LVCEPTRAAT VNVTSGISQN
510 520 530 540 550
FGRLVYGRHE GWSNLGDKTI QVMTYGSALA AWKVDNKFLS QFDAVFLDES
560 570 580 590 600
HLITTHALVF ESICQEVTNV RKFYVSATPR DGKKCPEAVR RYEIKTVKSE
610 620 630 640 650
CSSVDTFVRS QDKENSLYVL QHETVLVFLA GKAECDRAAS NWNKLYSTNM
660 670 680 690 700
YAYSLTGDNF TVAYENIVTR MLTDRIIVFC TNILETGVTL NVDCVVDFGF
710 720 730 740 750
TMRPELDLVD KSLTLMRRRV TENERAQRIG RAGRLRTGHA ICIGNPETRH
760 770 780 790 800
DLVPPETLYE AALLSFVHGV QFYINEHFEN AWIEGVTKSQ ASVMTQFKLS
810 820 830 840 850
PFLMRDIVRD DGAIPLSLHK ILKNYTHRNT DLIGTKLSVM SHVYNSWPLY
860 870 880 890 900
RTVHQSIFRG DSNVPQALKH ARVPFNVSTA HDFPWENFAQ ACLEFQPRVL
910 920 930 940 950
QVFSDSSSTS RIINLQIGKM HIVNSMEEVK ININSYQRSA ENLRSVKDSF
960 970 980 990 1000
ESSIFRTKLL RGNPTGKITK RIETLLDNVR VLQQVHAKLE IIAYSGGEKL
1010 1020 1030 1040 1050
NMDKKSVDEL NEIVELQSKN SLTAEELARI LHLTKPTSTF FNLFAERGRQ
1060 1070 1080 1090 1100
MLVTLLVMVA ASLMYLVFWV SPRKQDDITI EGKGRAYNRD KRMGYDSYEE
1110 1120 1130 1140 1150
DEVRHKINKK FKERSTRFSN DSKPETSSKY RNLKQEFVNF YDLKTDANVL
1160 1170 1180 1190 1200
QAVFTAMDGA VLLQTEAPMA DIDRVNRLLN DHFEDSESQA AHEGLNTMVK
1210 1220 1230 1240 1250
CHLTMKDGRQ FELDMEQHDP ETIAKLGGEV GFRMNRYDLR QVGATRYINP
1260 1270 1280 1290 1300
KAQTSAATLE GMTMKPMSAF TIDSAKMVGF IKTAKDTLNC ILYGDWIIAP
1310 1320 1330 1340 1350
AHIQQGEGDI TFIFQHTQFT TTTERLASYG IRQFKGLDLV VIRRPQQIRA
1360 1370 1380 1390 1400
VKKDMRASIL DTPTEVQMLY LSTKGGKYQV STSAVCFPHY NNRWGHVIST
1410 1420 1430 1440 1450
AEGMCGCIVF NPTTNHIVGI HVSYNDTRRR NEFQAFTSDV LTTINAPGHE
1460 1470 1480 1490 1500
IPFSPWVFDW KFCGYTTKPR NMQSAPSTLE RLNINATGFG FKLNAQGIKP
1510 1520 1530 1540 1550
AMLRSTETFS REFPNTQFKL IGEVKKGLID KHTITGENPY FLEFLNTFKP
1560 1570 1580 1590 1600
YQWVQAFMDE YAPSILAYDA YFKDLKKYDR PPHASVFCED TLTKAKHKMI
1610 1620 1630 1640 1650
KILEEAGMGR TLVRTTEQVL LDMAWTTSGG PLYHGKKIDI VQHLSDDELV
1660 1670 1680 1690 1700
QFSEACQQAL ITGTLDGVWN GSLKAELRSS QKILERKTRV FTAAPITSLI
1710 1720 1730 1740 1750
AMKYYVDDFN KQFYKTHLKA PHTVGINKFN RGWQNLYEKL NKPGWTHGSG
1760 1770 1780 1790 1800
DGSRFDSSID GFLFDVIKDI RKHFMDAEHH KQLDTIYEEI VNTKICLANG
1810 1820 1830 1840 1850
LVIQKNCGNN SGQPSTVVDN TLALMTSFLY AYARLTGDDT FELMDENFVF
1860 1870 1880 1890 1900
VCNGDDNKFA MSPSFMVKFG CDFSPFLSEL GLTYEFDEAT EDICENPYMS
1910 1920 1930 1940 1950
LTMVRTSFGI GFSLSIERIV AILQWSRAGG VLHAYLSGIA ALFESFNTPK
1960 1970 1980 1990 2000
LFNLVHTYLL WLVTEHEEEL FSMMELKDMF MPLPTKEQIA LLHYVGTEPI
2010 2020 2030 2040 2050
MEETYLQSGK DDPDPIVPPV SDTDLTNMAA APPDNRRSRA VVPRGTSDWN
2060 2070 2080 2090 2100
LPEPKMRMLG FKSKINIETL ADVPEGYMNT FASVATETQR RKWEEATRGD
2110 2120 2130 2140 2150
FGITDNEKWE KLLIAACIYF ADNGTSPNFD EELTMEVNGG LNSIKEYPVR
2160 2170 2180 2190 2200
PFVVRAKKIS TLRRIFRCYS IETKLMFVKL RRVPQWAIKH GCLDEIVFDF
2210 2220 2230 2240 2250
MIPDQFTSRT ALETLKQTKL AAIGVGTSNS LLTSEQTNMR TTETRRRNDY
DGHEALLR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D83408 Genomic RNA Translation: BAA18953.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D83408 Genomic RNA Translation: BAA18953.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Proteomic databases
PRIDEi | P90245 |
Family and domain databases
Gene3Di | 2.40.10.10, 1 hit 3.30.70.270, 1 hit 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR043502, DNA/RNA_pol_sf IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001592, Poty_coat IPR001730, Potyv_NIa-pro_dom IPR013648, PP_Potyviridae IPR043128, Rev_trsase/Diguanyl_cyclase IPR001205, RNA-dir_pol_C IPR007094, RNA-dir_pol_PSvirus |
Pfami | View protein in Pfam PF00271, Helicase_C, 1 hit PF00863, Peptidase_C4, 1 hit PF00767, Poty_coat, 1 hit PF08440, Poty_PP, 1 hit PF00680, RdRP_1, 1 hit |
PRINTSi | PR00966, NIAPOTYPTASE |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF52540, SSF52540, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51436, POTYVIRUS_NIA_PRO, 1 hit PS50507, RDRP_SSRNA_POS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | POL1_BAMMN | |
Accessioni | P90245Primary (citable) accession number: P90245 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 13, 2004 |
Last sequence update: | May 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 119 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |