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Protein

General transcription and DNA repair factor IIH subunit tfb2

Gene

tfb2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processDNA damage, DNA repair, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-SPO-113418 Formation of the Early Elongation Complex
R-SPO-5696395 Formation of Incision Complex in GG-NER
R-SPO-5696400 Dual Incision in GG-NER
R-SPO-674695 RNA Polymerase II Pre-transcription Events
R-SPO-6781823 Formation of TC-NER Pre-Incision Complex
R-SPO-6782135 Dual incision in TC-NER
R-SPO-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-SPO-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-SPO-72086 mRNA Capping
R-SPO-73776 RNA Polymerase II Promoter Escape
R-SPO-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-SPO-75953 RNA Polymerase II Transcription Initiation
R-SPO-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-SPO-77075 RNA Pol II CTD phosphorylation and interaction with CE

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription and DNA repair factor IIH subunit tfb2
Short name:
TFIIH subunit tfb2
Alternative name(s):
RNA polymerase II transcription factor B subunit 2
Gene namesi
Name:tfb2
ORF Names:SPBC13G1.13, SPBC31F10.01
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC13G1.13
PomBaseiSPBC13G1.13 tfb2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001192661 – 447General transcription and DNA repair factor IIH subunit tfb2Add BLAST447

Proteomic databases

MaxQBiP87303
PaxDbiP87303
PRIDEiP87303

Interactioni

Subunit structurei

Component of the 7-subunit TFIIH core complex composed of XPB/ptr8, XPD/rad15, ssl1, tfb1, tfb2, tfb4 and tfb5, which is active in NER. The core complex associates with the 3-subunit CTD-kinase module TFIIK composed of mcs2/cyclin H, mcs6/cdk7 and pmh1/tfb3 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription.1 Publication

Protein-protein interaction databases

BioGridi276340, 5 interactors
IntActiP87303, 4 interactors
STRINGi4896.SPBC13G1.13.1

Structurei

3D structure databases

ProteinModelPortaliP87303
SMRiP87303
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TFB2 family.Curated

Phylogenomic databases

HOGENOMiHOG000170529
InParanoidiP87303
KOiK03144
OMAiYLEQYAH
OrthoDBiEOG092C3703
PhylomeDBiP87303

Family and domain databases

InterProiView protein in InterPro
IPR004598 TFIIH_p52/Tfb2
PANTHERiPTHR13152 PTHR13152, 1 hit
PfamiView protein in Pfam
PF03849 Tfb2, 1 hit
TIGRFAMsiTIGR00625 tfb2, 1 hit

Sequencei

Sequence statusi: Complete.

P87303-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQAEFKSSIN DFLEQLPNHA RLYQKPAACL AVFRLLPILA RQYVMSMLFN
60 70 80 90 100
PMPVALSDFD LWIKLSSKVY QSESFNKLVR MHIFQFDGQY ITLNSEFRKQ
110 120 130 140 150
FITALTGGGN HNSFGVPCTD EDKHLVTVDF LDAYAKETWE TILHFMVGTP
160 170 180 190 200
EAKFPGEGVL SLLKRGGLMS GPKNQLRITR AGFQFLLQDI NAQIWTLLLD
210 220 230 240 250
YLKLSEDTHM DPVQVLHFLF MLGSLDLGRA YSVDFLTDTQ QIMLEDLREY
260 270 280 290 300
GLVYQRKITS KRFYPTRLAT GLTTDYRSLH GKQSENDDDK GFIIVETNYR
310 320 330 340 350
LYAYTSSPLQ IAIIGLFANL RARFSNLVVG VITRDSIRRA LMNGIAAEQI
360 370 380 390 400
ITYLTTHAHP QMRSNVPLLP PTLVDQIYLW ELEKNRLRAT PGILFRDFLT
410 420 430 440
DSGFDQAVEY AKELGVLVWD SSLKRMFFIT TTGAQPMIAY LKRRAVK
Length:447
Mass (Da):51,192
Last modified:May 1, 1999 - v2
Checksum:i2090A6187E393F40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA Translation: CAA18666.1
PIRiT39414
RefSeqiNP_596563.1, NM_001022484.2

Genome annotation databases

EnsemblFungiiSPBC13G1.13.1; SPBC13G1.13.1:pep; SPBC13G1.13
GeneIDi2539790
KEGGispo:SPBC13G1.13

Similar proteinsi

Entry informationi

Entry nameiTFB2_SCHPO
AccessioniPrimary (citable) accession number: P87303
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: May 1, 1999
Last modified: February 28, 2018
This is version 112 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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