UniProtKB - P87228 (SERA_SCHPO)
Protein
Putative D-3-phosphoglycerate dehydrogenase
Gene
SPCC364.07
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Functioni
Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate.By similarity
Catalytic activityi
: L-serine biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-serine from 3-phospho-D-glycerate.Proteins known to be involved in the 3 steps of the subpathway in this organism are:
- Putative D-3-phosphoglycerate dehydrogenase (SPCC364.07)
- Putative phosphoserine aminotransferase (SPAC1F12.07)
- Probable phosphoserine phosphatase (SPBC3H7.07c)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-serine from 3-phospho-D-glycerate, the pathway L-serine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 225 | NADBy similarity | 1 | |
Active sitei | 284 | By similarity | 1 | |
Binding sitei | 308 | NADBy similarity | 1 | |
Active sitei | 313 | By similarity | 1 | |
Active sitei | 344 | Proton donorBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 205 – 206 | NADBy similarity | 2 | |
Nucleotide bindingi | 282 – 284 | NADBy similarity | 3 | |
Nucleotide bindingi | 344 – 347 | NADBy similarity | 4 |
GO - Molecular functioni
- glyoxylate reductase (NADP) activity Source: GO_Central
- hydroxypyruvate reductase activity Source: GO_Central
- NAD binding Source: InterPro
- phosphoglycerate dehydrogenase activity Source: PomBase
GO - Biological processi
- L-serine biosynthetic process Source: PomBase
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Amino-acid biosynthesis, Serine biosynthesis |
Ligand | NAD |
Enzyme and pathway databases
UniPathwayi | UPA00135;UER00196 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | ORF Names:SPCC364.07, SPCC4G3.01 |
Organismi | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
Taxonomic identifieri | 284812 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › |
Proteomesi |
|
Organism-specific databases
PomBasei | SPCC364.07 |
VEuPathDBi | FungiDB:SPCC364.07 |
Subcellular locationi
Cytosol
- cytosol Source: PomBase
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000076017 | 1 – 466 | Putative D-3-phosphoglycerate dehydrogenaseAdd BLAST | 466 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 87 | Phosphoserine1 Publication | 1 | |
Modified residuei | 258 | Phosphoserine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P87228 |
PaxDbi | P87228 |
PRIDEi | P87228 |
PTM databases
iPTMneti | P87228 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 276053, 10 interactors |
STRINGi | 4896.SPCC364.07.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 396 – 466 | ACTPROSITE-ProRule annotationAdd BLAST | 71 |
Sequence similaritiesi
Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.Curated
Phylogenomic databases
eggNOGi | KOG0068, Eukaryota |
HOGENOMi | CLU_019796_9_2_1 |
InParanoidi | P87228 |
OMAi | HRGTWNK |
PhylomeDBi | P87228 |
Family and domain databases
InterProi | View protein in InterPro IPR002912, ACT_dom IPR006139, D-isomer_2_OHA_DH_cat_dom IPR029753, D-isomer_DH_CS IPR029752, D-isomer_DH_CS1 IPR006140, D-isomer_DH_NAD-bd IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF00389, 2-Hacid_dh, 1 hit PF02826, 2-Hacid_dh_C, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS51671, ACT, 1 hit PS00065, D_2_HYDROXYACID_DH_1, 1 hit PS00670, D_2_HYDROXYACID_DH_2, 1 hit PS00671, D_2_HYDROXYACID_DH_3, 1 hit |
i Sequence
Sequence statusi: Complete.
P87228-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDIKGGRRGN VEDSLNKLSL SPPDNNSSFL SNHFQVRKSY SQAPARTLKP
60 70 80 90 100
FASEDIKILL LENVNQSALS NLKDEGYQVE FLKTSMSEDD LVEKIKGVHA
110 120 130 140 150
IGIRSKTRLT RRVLEAADSL IVIGCFCIGT NQVDLDFAAE RGIAVFNSPY
160 170 180 190 200
ANSRSVAELV IGYIISLARQ VGDRSLELHR GEWNKVSSGC WEIRGKTLGI
210 220 230 240 250
IGYGHIGSQL SVLAEAMGLH VVYYDILPIM PLGSAKQLSS LPELLHRADF
260 270 280 290 300
VSLHVPASPE TKNMISSKEF AAMKEGSYLI NASRGTVVDI PALVDASKSG
310 320 330 340 350
KIAGAAIDVY PSEPAGNGKD KFVDSLNSWT SELTHCKNII LTPHIGGSTE
360 370 380 390 400
EAQYNIGIEV SEALTRYINE GNSIGAVNFP EVSLRSLTEA DRNAARVLFV
410 420 430 440 450
HRNVPGVLRQ VNELFIDHNI KSQFSDSRGD IAYLVADISD CTPGSLEALH
460
QKLESLPCKI NTRLLY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU329672 Genomic DNA Translation: CAB09778.1 |
PIRi | T41375 |
RefSeqi | NP_587837.1, NM_001022830.2 |
Genome annotation databases
EnsemblFungii | SPCC364.07.1; SPCC364.07.1:pep; SPCC364.07 |
GeneIDi | 2539490 |
KEGGi | spo:SPCC364.07 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU329672 Genomic DNA Translation: CAB09778.1 |
PIRi | T41375 |
RefSeqi | NP_587837.1, NM_001022830.2 |
3D structure databases
SMRi | P87228 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 276053, 10 interactors |
STRINGi | 4896.SPCC364.07.1 |
PTM databases
iPTMneti | P87228 |
Proteomic databases
MaxQBi | P87228 |
PaxDbi | P87228 |
PRIDEi | P87228 |
Genome annotation databases
EnsemblFungii | SPCC364.07.1; SPCC364.07.1:pep; SPCC364.07 |
GeneIDi | 2539490 |
KEGGi | spo:SPCC364.07 |
Organism-specific databases
PomBasei | SPCC364.07 |
VEuPathDBi | FungiDB:SPCC364.07 |
Phylogenomic databases
eggNOGi | KOG0068, Eukaryota |
HOGENOMi | CLU_019796_9_2_1 |
InParanoidi | P87228 |
OMAi | HRGTWNK |
PhylomeDBi | P87228 |
Enzyme and pathway databases
UniPathwayi | UPA00135;UER00196 |
Miscellaneous databases
PROi | PR:P87228 |
Family and domain databases
InterProi | View protein in InterPro IPR002912, ACT_dom IPR006139, D-isomer_2_OHA_DH_cat_dom IPR029753, D-isomer_DH_CS IPR029752, D-isomer_DH_CS1 IPR006140, D-isomer_DH_NAD-bd IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF00389, 2-Hacid_dh, 1 hit PF02826, 2-Hacid_dh_C, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS51671, ACT, 1 hit PS00065, D_2_HYDROXYACID_DH_1, 1 hit PS00670, D_2_HYDROXYACID_DH_2, 1 hit PS00671, D_2_HYDROXYACID_DH_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SERA_SCHPO | |
Accessioni | P87228Primary (citable) accession number: P87228 Secondary accession number(s): O59798 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | July 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 152 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families - Schizosaccharomyces pombe
Schizosaccharomyces pombe: entries and gene names