UniProtKB - P87184 (ALP2_ASPFU)
Protein
Alkaline protease 2
Gene
alp2
Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Functioni
Alkaline protease that allows assimilation of proteinaceous substrates. Acts as a significant virulence factor in invasive aspergillosis. Required for regular sporulation.1 Publication
Catalytic activityi
- Hydrolysis of proteins with broad specificity, and of Bz-Arg-OEt > Ac-Tyr-OEt. Does not hydrolyze peptide amides. EC:3.4.21.63
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 182 | Charge relay systemPROSITE-ProRule annotation | 1 | |
Active sitei | 214 | Charge relay systemPROSITE-ProRule annotation | 1 | |
Active sitei | 380 | Charge relay systemPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- IgE binding Source: UniProtKB
- serine-type endopeptidase activity Source: GO_Central
GO - Biological processi
- asexual sporulation resulting in formation of a cellular spore Source: AspGD
- pathogenesis Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Biological process | Sporulation, Virulence |
Names & Taxonomyi
Protein namesi | Recommended name: Alkaline protease 2 (EC:3.4.21.63)Short name: ALP2 Alternative name(s): Autophagic serine protease alp2 Allergen: Asp f 18 |
Gene namesi | Name:alp2 ORF Names:AFUA_5G09210 |
Organismi | Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus) |
Taxonomic identifieri | 330879 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Aspergillaceae › Aspergillus › Aspergillus subgen. Fumigati › |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | FungiDB:Afu5g09210 |
Subcellular locationi
Extracellular region or secreted
- extracellular space Source: GO_Central
Pathology & Biotechi
Allergenic propertiesi
Acts as a major allergen in patients suffering from extrinsic bronchial asthma. Binds to IgE.1 Publication
Keywords - Diseasei
AllergenProtein family/group databases
Allergomei | 3114, Asp f 18.0101 70, Asp f 18 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 16 | Sequence analysisAdd BLAST | 16 | |
PropeptideiPRO_0000412304 | 17 – 136 | 1 PublicationAdd BLAST | 120 | |
ChainiPRO_0000412305 | 137 – 495 | Alkaline protease 2Add BLAST | 359 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 284 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 447 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 460 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Glycoprotein, ZymogenFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 43 – 136 | Inhibitor I9Sequence analysisAdd BLAST | 94 | |
Domaini | 146 – 452 | Peptidase S8PROSITE-ProRule annotationAdd BLAST | 307 |
Sequence similaritiesi
Belongs to the peptidase S8 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG1153, Eukaryota |
HOGENOMi | CLU_011263_1_4_1 |
InParanoidi | P87184 |
OMAi | WIGSKHA |
OrthoDBi | 921536at2759 |
Family and domain databases
CDDi | cd04077, Peptidases_S8_PCSK9_ProteinaseK_like, 1 hit |
Gene3Di | 3.30.70.80, 1 hit 3.40.50.200, 1 hit |
InterProi | View protein in InterPro IPR034193, PCSK9_ProteinaseK-like IPR000209, Peptidase_S8/S53_dom IPR036852, Peptidase_S8/S53_dom_sf IPR022398, Peptidase_S8_His-AS IPR023828, Peptidase_S8_Ser-AS IPR015500, Peptidase_S8_subtilisin-rel IPR010259, S8pro/Inhibitor_I9 IPR037045, S8pro/Inhibitor_I9_sf |
Pfami | View protein in Pfam PF05922, Inhibitor_I9, 1 hit PF00082, Peptidase_S8, 1 hit |
PRINTSi | PR00723, SUBTILISIN |
SUPFAMi | SSF52743, SSF52743, 1 hit |
PROSITEi | View protein in PROSITE PS51892, SUBTILASE, 1 hit PS00137, SUBTILASE_HIS, 1 hit PS00138, SUBTILASE_SER, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P87184-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKGYLSLSIL PLLVAASPVV VDSIHNGAAP ILSSMNAKEV PDSYIVVFKK
60 70 80 90 100
HVNAESAAAH HSWVQDIHSA QNERVELRKR SLFGFGEEAY LGLKNTFDIA
110 120 130 140 150
GSLVGYSGHF HEDVIEQVRK HPDVEYIEKD SEVHTMEDPT VEKSAPWGLA
160 170 180 190 200
RISHRDSLSF GTFNKYLYAS EGGEGVDAYT IDTGINVDHV DFEGRAQWGK
210 220 230 240 250
TIPTDDEDAD GNGHGTHCSG TIAGRKYGVA KKANLYAVKV LRSSGSGTMS
260 270 280 290 300
DVVAGVEWAV KSHLKKVKDA KDGKIKGFKG SVANMSLGGG KSRTLEAAVN
310 320 330 340 350
AGVEAGLHFA VAAGNDNADA CNYSPAAAEN PITVGASTLQ DERAYFSNYG
360 370 380 390 400
KCTDIFAPGL NILSTWIGSK HAVNTISGTS MASPHIAGLL AYFVSLQPSK
410 420 430 440 450
DSAFAVDELT PKKLKKDIIA IATQGALTDI PSDTPNLLAW NGGGSSNYTD
460 470 480 490
IIASGGYKVN ASVKDRFEGL VHKAEKLLTE ELGAIYSEIH DAAVA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y13338 mRNA Translation: CAA73782.1 AJ243145 Genomic DNA Translation: CAB45520.1 AAHF01000003 Genomic DNA Translation: EAL91680.1 |
RefSeqi | XP_753718.1, XM_748625.1 |
Genome annotation databases
EnsemblFungii | EAL91680; EAL91680; AFUA_5G09210 |
GeneIDi | 3510885 |
KEGGi | afm:AFUA_5G09210 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y13338 mRNA Translation: CAA73782.1 AJ243145 Genomic DNA Translation: CAB45520.1 AAHF01000003 Genomic DNA Translation: EAL91680.1 |
RefSeqi | XP_753718.1, XM_748625.1 |
3D structure databases
SMRi | P87184 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 746128.CADAFUBP00005557 |
Protein family/group databases
Allergomei | 3114, Asp f 18.0101 70, Asp f 18 |
Genome annotation databases
EnsemblFungii | EAL91680; EAL91680; AFUA_5G09210 |
GeneIDi | 3510885 |
KEGGi | afm:AFUA_5G09210 |
Organism-specific databases
VEuPathDBi | FungiDB:Afu5g09210 |
Phylogenomic databases
eggNOGi | KOG1153, Eukaryota |
HOGENOMi | CLU_011263_1_4_1 |
InParanoidi | P87184 |
OMAi | WIGSKHA |
OrthoDBi | 921536at2759 |
Family and domain databases
CDDi | cd04077, Peptidases_S8_PCSK9_ProteinaseK_like, 1 hit |
Gene3Di | 3.30.70.80, 1 hit 3.40.50.200, 1 hit |
InterProi | View protein in InterPro IPR034193, PCSK9_ProteinaseK-like IPR000209, Peptidase_S8/S53_dom IPR036852, Peptidase_S8/S53_dom_sf IPR022398, Peptidase_S8_His-AS IPR023828, Peptidase_S8_Ser-AS IPR015500, Peptidase_S8_subtilisin-rel IPR010259, S8pro/Inhibitor_I9 IPR037045, S8pro/Inhibitor_I9_sf |
Pfami | View protein in Pfam PF05922, Inhibitor_I9, 1 hit PF00082, Peptidase_S8, 1 hit |
PRINTSi | PR00723, SUBTILISIN |
SUPFAMi | SSF52743, SSF52743, 1 hit |
PROSITEi | View protein in PROSITE PS51892, SUBTILASE, 1 hit PS00137, SUBTILASE_HIS, 1 hit PS00138, SUBTILASE_SER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ALP2_ASPFU | |
Accessioni | P87184Primary (citable) accession number: P87184 Secondary accession number(s): E9QST4, Q4WUP6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 21, 2011 |
Last sequence update: | July 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries