UniProtKB - P87115 (NAT10_SCHPO)
Protein
RNA cytidine acetyltransferase
Gene
nat10
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Functioni
RNA cytidine acetyltransferase with specificity toward both 18S rRNA and tRNAs. Catalyzes the formation of N4-acetylcytidine (ac4C) at positions 1297 and 1815 in 18S rRNA. Required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis (PubMed:25402480). Catalyzes the formation of ac4C in serine and leucine tRNAs. Requires the tRNA-binding adapter protein tan1 for full tRNA acetyltransferase activity but not for 18S rRNA acetylation (By similarity).UniRule annotationBy similarity1 Publication
Catalytic activityi
- a cytidine in 18S rRNA + acetyl-CoA + ATP + H2O = ADP + an N4-acetylcytidine in 18S rRNA + CoA + H+ + phosphateUniRule annotation1 Publication
- a cytidine in tRNA + acetyl-CoA + ATP + H2O = ADP + an N4-acetylcytidine in tRNA + CoA + H+ + phosphateUniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 465 | ATPUniRule annotation | 1 | |
Binding sitei | 727 | Acetyl-CoAUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 285 – 294 | ATPUniRule annotation | 10 |
GO - Molecular functioni
- ATP binding Source: PomBase
- rRNA cytidine N-acetyltransferase activity Source: PomBase
- tRNA binding Source: GO_Central
- tRNA N-acetyltransferase activity Source: PomBase
GO - Biological processi
- rRNA acetylation involved in maturation of SSU-rRNA Source: PomBase
- tRNA acetylation Source: PomBase
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | rRNA processing, tRNA processing |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: RNA cytidine acetyltransferase1 PublicationUniRule annotation (EC:2.3.1.-UniRule annotation)Alternative name(s): 18S rRNA cytosine acetyltransferaseUniRule annotation |
Gene namesi | ORF Names:SPAC20G8.09cImported |
Organismi | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
Taxonomic identifieri | 284812 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › |
Proteomesi |
|
Organism-specific databases
PomBasei | SPAC20G8.09c, nat10 |
VEuPathDBi | FungiDB:SPAC20G8.09c |
Subcellular locationi
Nucleus
- nucleolus UniRule annotation1 Publication
Nucleus
Other locations
- 90S preribosome Source: PomBase
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 285 | G → D: Completely lacks 18S rRNA acetylation and produces only trace amounts of the 40S small ribosomal subunit. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000215889 | 1 – 1033 | RNA cytidine acetyltransferaseAdd BLAST | 1033 |
Proteomic databases
MaxQBi | P87115 |
PaxDbi | P87115 |
PRIDEi | P87115 |
PTM databases
iPTMneti | P87115 |
SwissPalmi | P87115 |
Interactioni
Subunit structurei
Interacts with tan1.
UniRule annotationProtein-protein interaction databases
BioGRIDi | 278978, 13 interactors |
STRINGi | 4896.SPAC20G8.09c.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 560 – 694 | N-acetyltransferaseUniRule annotationAdd BLAST | 135 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 626 – 628 | Acetyl-CoA bindingUniRule annotation | 3 | |
Regioni | 633 – 639 | Acetyl-CoA bindingUniRule annotation | 7 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2036, Eukaryota |
HOGENOMi | CLU_004652_0_0_1 |
InParanoidi | P87115 |
OMAi | FWKRASF |
PhylomeDBi | P87115 |
Family and domain databases
HAMAPi | MF_03211, RNA_acetyltr_Nat10, 1 hit |
InterProi | View protein in InterPro IPR000182, GNAT_dom IPR007807, Helicase_dom IPR033688, NAT10 IPR032672, TmcA/NAT10/Kre33 IPR013562, TmcA_N IPR027992, tRNA_bind_dom |
PANTHERi | PTHR10925, PTHR10925, 1 hit |
Pfami | View protein in Pfam PF08351, DUF1726, 1 hit PF13718, GNAT_acetyltr_2, 1 hit PF05127, Helicase_RecD, 1 hit PF13725, tRNA_bind_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P87115-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPKKALDSRI PTLIKNGCQE KQRSFFVVVG DRARDQVVNL HWLLSQSKVA
60 70 80 90 100
ARPNVLWMYK KDLLGFTSHR KKRENKIKKE IKRGIRDPNS EDPFELFCSI
110 120 130 140 150
TNIRYCYYKE SEKILGQTYG MLVLQDFEAL TPNLLARTIE TVEGGGIVVL
160 170 180 190 200
LLHKLNSLKQ LYTMSMDIHS RYRTEAHSDV TARFNERFIL SLGNCENCLV
210 220 230 240 250
IDDELNVLPI SGGKNVKALP PTLEEDNSTQ NSIKELQESL GEDHPAGALV
260 270 280 290 300
GVTKTLDQAR AVLTFVESIV EKSLKGTVSL TAGRGRGKSA ALGLAIAAAI
310 320 330 340 350
AHGYSNIFIT SPSPENLKTL FEFIFKGFDA LNYEEHVDYD IIQSTNPAYH
360 370 380 390 400
NAIVRVNIFR DHRQTIQYIS PEDSNVLGQA ELVVIDEAAA IPLPLVRKLI
410 420 430 440 450
GPYLVFMAST INGYEGTGRS LSLKLLQQLR EQSRIYSGSG NNKSDSQSHI
460 470 480 490 500
SGRTLKEISL DEPIRYAMGD RIELWLNKLL CLDAASYVSR MATQGFPHPS
510 520 530 540 550
ECSLYRVSRD TLFSYHPISE AFLQRMMSLY VASHYKNSPN DLQLMSDAPA
560 570 580 590 600
HQLFVLLPPV DLKNPKLPDP ICVIQLALEG SISRESIMNS LSRGQRAGGD
610 620 630 640 650
LIPWLISQQF QDENFAALGG ARIVRIAVSP EHVKMGYGTR AMQLLHEYFE
660 670 680 690 700
GKFISASEEF KAVKHSLKRI GDEEIENTAL QTEKIHVRDA KTMPPLLLKL
710 720 730 740 750
SELQPEPLHY VGVSYGLTPS LQKFWKREGY CPLYLRQTAN DLTGEHTCVM
760 770 780 790 800
LRVLEGRDSE WLGAFAQNFY RRFLSLLGYQ FREFAAITAL SVLDACNNGT
810 820 830 840 850
KYVVNSTSKL TNEEINNVFE SYDLKRLESY SNNLLDYHVI VDLLPKLAHL
860 870 880 890 900
YFSGKFPDSV KLSPVQQSVL LALGLQYKTI DTLEKEFNLP SNQLLAMLVK
910 920 930 940 950
LSKKIMKCID EIETKDIEEE LGSNKKTESS NSKLPEFTPL QQSLEEELQE
960 970 980 990 1000
GADEAMLALR EKQRELINAI DLEKYAIRGN EEDWKAAENQ IQKTNGKGAR
1010 1020 1030
VVSIKGEKRK NNSLDASDKK TKEKPSSKKK FRK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU329670 Genomic DNA Translation: CAB08603.1 |
PIRi | T38131 |
RefSeqi | NP_593326.1, NM_001018757.2 |
Genome annotation databases
EnsemblFungii | SPAC20G8.09c.1; SPAC20G8.09c.1:pep; SPAC20G8.09c |
GeneIDi | 2542520 |
KEGGi | spo:SPAC20G8.09c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU329670 Genomic DNA Translation: CAB08603.1 |
PIRi | T38131 |
RefSeqi | NP_593326.1, NM_001018757.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 278978, 13 interactors |
STRINGi | 4896.SPAC20G8.09c.1 |
PTM databases
iPTMneti | P87115 |
SwissPalmi | P87115 |
Proteomic databases
MaxQBi | P87115 |
PaxDbi | P87115 |
PRIDEi | P87115 |
Genome annotation databases
EnsemblFungii | SPAC20G8.09c.1; SPAC20G8.09c.1:pep; SPAC20G8.09c |
GeneIDi | 2542520 |
KEGGi | spo:SPAC20G8.09c |
Organism-specific databases
PomBasei | SPAC20G8.09c, nat10 |
VEuPathDBi | FungiDB:SPAC20G8.09c |
Phylogenomic databases
eggNOGi | KOG2036, Eukaryota |
HOGENOMi | CLU_004652_0_0_1 |
InParanoidi | P87115 |
OMAi | FWKRASF |
PhylomeDBi | P87115 |
Miscellaneous databases
PROi | PR:P87115 |
Family and domain databases
HAMAPi | MF_03211, RNA_acetyltr_Nat10, 1 hit |
InterProi | View protein in InterPro IPR000182, GNAT_dom IPR007807, Helicase_dom IPR033688, NAT10 IPR032672, TmcA/NAT10/Kre33 IPR013562, TmcA_N IPR027992, tRNA_bind_dom |
PANTHERi | PTHR10925, PTHR10925, 1 hit |
Pfami | View protein in Pfam PF08351, DUF1726, 1 hit PF13718, GNAT_acetyltr_2, 1 hit PF05127, Helicase_RecD, 1 hit PF13725, tRNA_bind_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NAT10_SCHPO | |
Accessioni | P87115Primary (citable) accession number: P87115 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | July 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 132 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Schizosaccharomyces pombe
Schizosaccharomyces pombe: entries and gene names - SIMILARITY comments
Index of protein domains and families