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UniProtKB - P87014 (MAM4_SCHPO)
Protein
Protein-S-isoprenylcysteine O-methyltransferase
Gene
mam4
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Functioni
Mediates C-terminal methylation of the isoprenylated C-terminal cysteine in M-factor.
1 PublicationCatalytic activityi
- [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine + S-adenosyl-L-methionine = [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine methyl ester + S-adenosyl-L-homocysteine1 PublicationEC:2.1.1.1001 PublicationThis reaction proceeds in the forward1 Publication direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 163 | S-adenosyl-L-methionineBy similarity | 1 | |
Binding sitei | 205 | SubstrateBy similarity | 1 | |
Binding sitei | 209 | S-adenosyl-L-methionineBy similarity | 1 |
GO - Molecular functioni
GO - Biological processi
- C-terminal protein methylation Source: GO_Central
- peptide mating pheromone maturation involved in positive regulation of conjugation with cellular fusion Source: PomBase
- response to pheromone Source: UniProtKB-KW
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | Pheromone response |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
Reactomei | R-SPO-163841, Gamma carboxylation, hypusine formation and arylsulfatase activation |
Names & Taxonomyi
Protein namesi | Recommended name: Protein-S-isoprenylcysteine O-methyltransferase (EC:2.1.1.1001 Publication)Alternative name(s): Isoprenylcysteine carboxylmethyltransferase Prenylated protein carboxyl methyltransferase Short name: PPMT Prenylcysteine carboxyl methyltransferase Short name: pcCMT |
Gene namesi | Name:mam4 ORF Names:SPAC10F6.12c |
Organismi | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
Taxonomic identifieri | 284812 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › |
Proteomesi |
|
Organism-specific databases
PomBasei | SPAC10F6.12c, mam4 |
VEuPathDBi | FungiDB:SPAC10F6.12c |
Subcellular locationi
Other locations
- Membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum Source: PomBase
- endoplasmic reticulum membrane Source: PomBase
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 3 – 23 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 24 – 44 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 76 – 96 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 108 – 128 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 174 – 194 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000209898 | 1 – 236 | Protein-S-isoprenylcysteine O-methyltransferaseAdd BLAST | 236 |
Proteomic databases
MaxQBi | P87014 |
PaxDbi | P87014 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 279420, 5 interactors |
STRINGi | 4896.SPAC10F6.12c.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 155 – 158 | S-adenosyl-L-methionine bindingBy similarity | 4 | |
Regioni | 168 – 171 | S-adenosyl-L-methionine bindingBy similarity | 4 |
Sequence similaritiesi
Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2628, Eukaryota |
HOGENOMi | CLU_065200_0_2_1 |
InParanoidi | P87014 |
OMAi | FFYWGLG |
PhylomeDBi | P87014 |
Family and domain databases
InterProi | View protein in InterPro IPR007269, ICMT_MeTrfase IPR025770, PPMT_MeTrfase |
Pfami | View protein in Pfam PF04140, ICMT, 1 hit |
PROSITEi | View protein in PROSITE PS51564, SAM_ICMT, 1 hit |
i Sequence
Sequence statusi: Complete.
P87014-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGNLHTSIAV ASICLTSAFL GCVFGLGFFV WIIYGYSIGG FFAFLSLFHL
60 70 80 90 100
LEFYITARFQ GSQLSWDSFI LNNGKAYWLA MLVGLLECLL SGGKSFAKVI
110 120 130 140 150
NCLRFPSFLI NFIFSVYQTS ALGFLCLGQY LRSSAMVQAG QSFSHIVASK
160 170 180 190 200
RNKDHLLVTD GIYAYVRHPS YVGFFIWALG TQMLLGNFVS TLLFSLVLWK
210 220 230
FFSQRITTEE AYLVSFFGDS YEQYRKKVPS GIPLIP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D87749 Genomic DNA Translation: BAA18999.1 CU329670 Genomic DNA Translation: CAA15725.1 |
PIRi | T43237 |
RefSeqi | NP_593263.1, NM_001018660.2 |
Genome annotation databases
EnsemblFungii | SPAC10F6.12c.1; SPAC10F6.12c.1:pep; SPAC10F6.12c |
GeneIDi | 2542982 |
KEGGi | spo:SPAC10F6.12c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D87749 Genomic DNA Translation: BAA18999.1 CU329670 Genomic DNA Translation: CAA15725.1 |
PIRi | T43237 |
RefSeqi | NP_593263.1, NM_001018660.2 |
3D structure databases
SMRi | P87014 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 279420, 5 interactors |
STRINGi | 4896.SPAC10F6.12c.1 |
Proteomic databases
MaxQBi | P87014 |
PaxDbi | P87014 |
Genome annotation databases
EnsemblFungii | SPAC10F6.12c.1; SPAC10F6.12c.1:pep; SPAC10F6.12c |
GeneIDi | 2542982 |
KEGGi | spo:SPAC10F6.12c |
Organism-specific databases
PomBasei | SPAC10F6.12c, mam4 |
VEuPathDBi | FungiDB:SPAC10F6.12c |
Phylogenomic databases
eggNOGi | KOG2628, Eukaryota |
HOGENOMi | CLU_065200_0_2_1 |
InParanoidi | P87014 |
OMAi | FFYWGLG |
PhylomeDBi | P87014 |
Enzyme and pathway databases
Reactomei | R-SPO-163841, Gamma carboxylation, hypusine formation and arylsulfatase activation |
Miscellaneous databases
PROi | PR:P87014 |
Family and domain databases
InterProi | View protein in InterPro IPR007269, ICMT_MeTrfase IPR025770, PPMT_MeTrfase |
Pfami | View protein in Pfam PF04140, ICMT, 1 hit |
PROSITEi | View protein in PROSITE PS51564, SAM_ICMT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MAM4_SCHPO | |
Accessioni | P87014Primary (citable) accession number: P87014 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 20, 2001 |
Last sequence update: | July 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Schizosaccharomyces pombe
Schizosaccharomyces pombe: entries and gene names - SIMILARITY comments
Index of protein domains and families