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UniProtKB - P86491 (6HN3M_PSEFL)
Protein
6-hydroxynicotinate 3-monooxygenase
Gene
nicC
Organism
Pseudomonas fluorescens
Status
Functioni
Flavin-dependent monooxygenase (FMO) that catalyzes the decarboxylative hydroxylation of 6-hydroxynicotinic acid (6-HNA) to 2,5-dihydroxypyridine (2,5-DHP) with concomitant oxidation of NADH, a step in the aerobic nicotinate degradation pathway. Uses NADH in preference to NADPH as an electron donor.
1 PublicationCatalytic activityi
- EC:1.14.13.1141 Publication
Cofactori
FAD1 PublicationNote: Binds 1 FAD molecule per subunit.1 Publication
Activity regulationi
Inhibited competitively by nicotinic acid with a Ki of 0.49 mM. Inhibited by thiol-specific compounds p-chloromercuribenzoate, DTNB, Ag2SO4, HgCl2, CuCl2 and N-ethylmaleimide. No inhibition by o-phenanthroline, 8-hydroxyquinoline, EDTA, disodium 4,5-dihydroxy-m-benzenedisulfonate, fluoride, azide, KCl, LiCl, NaCl, BaCl2, MnCl2, MgCl2, PBCl, ZnCl2, CoCl2, SnCl2, FeSO4, FeCl3, NiCl2, CdCl2, AlCl3, iodoacetic acid, hydro-xylamine, phenylhydrazine, semicarbazide, cysteamine, alpha,alpha-dipyridyl and urea.1 Publication
Kineticsi
- KM=98 µM for 6-hydroxynicotinic acid (at 30 degrees Celsius and pH 7.0)1 Publication
- KM=150 µM for 4-hydroxybenzoic acid (at 30 degrees Celsius and pH 7.0)1 Publication
- Vmax=6.67 µmol/min/mg enzyme toward 6-hydroxynicotinic acid (at 30 degrees Celsius and pH 7.0)1 Publication
pH dependencei
Optimum pH is 7.6 with 6-hydroxynicotinic acid as substrate. Stable between pH 6.0 and 9.0.1 Publication
Temperature dependencei
Optimum temperature is 35 degrees Celsius with 6-hydroxynicotinic acid as substrate.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 15 | FAD; via amide nitrogenBy similarity | 1 | |
Active sitei | 47 | Proton acceptorBy similarity | 1 | |
Binding sitei | 47 | FAD; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Binding sitei | 108 | FADBy similarity | 1 | |
Binding sitei | 130 | FAD; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Active sitei | 215 | Proton acceptorBy similarity | 1 | |
Binding sitei | 294 | FADBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 34 – 35 | FADBy similarity | 2 | |
Nucleotide bindingi | 307 – 308 | FADBy similarity | 2 |
GO - Molecular functioni
- 6-hydroxynicotinate 3-monooxygenase activity Source: UniProtKB-EC
- FAD binding Source: InterPro
- monooxygenase activity Source: UniProtKB
GO - Biological processi
- aromatic compound catabolic process Source: UniProtKB
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Aromatic hydrocarbons catabolism |
Ligand | FAD, Flavoprotein, NAD, NADP |
Names & Taxonomyi
Protein namesi | Recommended name: 6-hydroxynicotinate 3-monooxygenase1 Publication (EC:1.14.13.1141 Publication)Short name: 6-HNA monooxygenase |
Gene namesi | Name:nicC |
Organismi | Pseudomonas fluorescens |
Taxonomic identifieri | 294 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Sequence analysisAdd BLAST | 20 | |
ChainiPRO_0000394198 | 21 – 385 | 6-hydroxynicotinate 3-monooxygenaseSequence analysisAdd BLAST | 365 |
Interactioni
Subunit structurei
Monomer.
1 PublicationFamily & Domainsi
Sequence similaritiesi
Belongs to the 6-hydroxynicotinate 3-monooxygenase family.Curated
Keywords - Domaini
SignalFamily and domain databases
Gene3Di | 3.50.50.60, 1 hit |
InterProi | View protein in InterPro IPR002938, FAD-bd IPR036188, FAD/NAD-bd_sf |
Pfami | View protein in Pfam PF01494, FAD_binding_3, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P86491-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSQSPRIAVV GAGLGGAAAA KLLLQEGFNV RVYEQAPSFS RLGAGIHVGP
60 70 80 90 100
NVMKILRRIG IEDALNEQGS HPDYWYSRHW QTGDVLAQIP LGDYAVKEYG
110 120 130 140 150
ASYLTVHRGD FHALLVEALP DSVMAYGKFL TKVEDRGNVV VMHFADGTTE
160 170 180 190 200
EADIVIGPDG VNSRIREELL GPELPKYAGY LAHRAVFPTP EVKAGMLPFD
210 220 230 240 250
ACVKWWSDDR HMMTYFVTGK ADELYYVTGV PVEKWDLNDR WLESSKEEMR
260 270 280 290 300
EAFSGWHPTV QALIDATVEV TKWSLLERDP LPLWSRGRLV LLGDACHPMK
310 320 330 340 350
PHMAQGAAMA IEDGAMLARC LKEVGAHNHE LAFALYEANR AERASKVQRI
360 370 380
SHDNTWLRTN EDPSWCFGYD VFNVPLVEPK VKAAA
Similar proteinsi
Cross-referencesi
3D structure databases
AlphaFoldDBi | P86491 |
SMRi | P86491 |
ModBasei | Search... |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
InterProi | View protein in InterPro IPR002938, FAD-bd IPR036188, FAD/NAD-bd_sf |
Pfami | View protein in Pfam PF01494, FAD_binding_3, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | 6HN3M_PSEFL | |
Accessioni | P86491Primary (citable) accession number: P86491 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 18, 2010 |
Last sequence update: | May 18, 2010 | |
Last modified: | May 25, 2022 | |
This is version 27 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- SIMILARITY comments
Index of protein domains and families