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Protein

Trimethylguanosine synthase

Gene

Tgs1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m7G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + m7G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(2,7)G(5')pppR-RNA.
S-adenosyl-L-methionine + m(2,7)G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(2,2,7)G(5')pppR-RNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei713S-adenosyl-L-methionineBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processTranscription, Transcription regulation
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-RNO-191859 snRNP Assembly
R-RNO-400206 Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha)

Names & Taxonomyi

Protein namesi
Recommended name:
Trimethylguanosine synthase (EC:2.1.1.-)
Alternative name(s):
Nuclear receptor coactivator 6-interacting protein
PRIP-interacting protein with methyltransferase motif
Short name:
PIMT
Short name:
PIPMT
Gene namesi
Name:Tgs1By similarity
Synonyms:Ncoa6ipImported, PimtBy similarity
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi1309953 Tgs1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002837271 – 850Trimethylguanosine synthaseAdd BLAST850

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei61PhosphothreonineBy similarity1
Modified residuei92PhosphoserineBy similarity1
Modified residuei152PhosphoserineBy similarity1
Modified residuei405PhosphoserineBy similarity1
Modified residuei431PhosphoserineCombined sources1
Modified residuei571PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP85107
PRIDEiP85107

PTM databases

iPTMnetiP85107
PhosphoSitePlusiP85107

Expressioni

Tissue specificityi

A 55 kDa isoform is widely expressed while a 90 kDa isoform is detected exclusively in brain and testis (at protein level).1 Publication

Gene expression databases

BgeeiENSRNOG00000008156 Expressed in 9 organ(s), highest expression level in skeletal muscle tissue
GenevisibleiP85107 RN

Interactioni

Subunit structurei

May form homooligomers. Interacts with CREBBP/CBP, EED/WAIT1, EP300/P300, NCOA6/PRIP, PPARBP/PBP and SMN (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010783

Structurei

3D structure databases

ProteinModelPortaliP85107
SMRiP85107
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi611 – 624Lys-richSequence analysisAdd BLAST14

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2730 Eukaryota
ENOG410XRUD LUCA
GeneTreeiENSGT00390000018056
HOGENOMiHOG000154561
HOVERGENiHBG059797
InParanoidiP85107
KOiK14292
OMAiMNTRNKV
OrthoDBiEOG091G0DYO
PhylomeDBiP85107
TreeFamiTF313065

Family and domain databases

InterProiView protein in InterPro
IPR019012 RNA_cap_Gua-N2-MeTrfase
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF09445 Methyltransf_15, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit

Sequencei

Sequence statusi: Complete.

P85107-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MCCEKWSHVA EMLLFIEDRE EEYKILCLCS RAFVEDRKLY NLGLKGYYVK
60 70 80 90 100
SSGNNAGDRV TEEEEDDHSS GTTESHSADE GDLDPEAKLM RSMGLPVRFG
110 120 130 140 150
RMSTHGSFEV SMNARNKAKV RQKRRKHRKQ YLDEIVREDW RNDCEEDDLV
160 170 180 190 200
VSDDPSSVEH CENNSACEIQ SKTDAEAENL PVENTLAPKL EVTENWEKYW
210 220 230 240 250
NEYGEGLLWQ SWQEKYPDQT LSSEPWNLPD TKEEWEQHYS QLYWYYLEQF
260 270 280 290 300
QYWEAQGWTF VASQNCDKDV CTSHTQVDQN AESSLKADGT TLSSSPNTAE
310 320 330 340 350
DESLGSNDND HNEIIAGINK ITLSAEEVEQ SQLDSSEHCD KPLSEVTGKE
360 370 380 390 400
CPASGGSDSC HGTPKESDIS ENRSSDQPAQ ELQESSGTNT SKHRPHHNGT
410 420 430 440 450
DGNESEDDPP DHKPSKMKRS HELDIDENPD SEVDDNGFHL GFKHGSGQKY
460 470 480 490 500
GGIPNFSRRQ VRYLEKNVKY KSKYLDMRKR MTVKNKHIVF SEESGKPFIR
510 520 530 540 550
KSKVRSKVEK FLKWVNEPVD EEISQEPLSH NKMQDTCTSS DSEEQDMSME
560 570 580 590 600
KTDGLMETRD PEPENCQTIS SASELEAEKS EGGSLVAAVP ENCSTEGVAN
610 620 630 640 650
SPRAEAEVEI KKKKKKKKKN KNKKINGLPP EIASVPELAK YWAQRYRLFS
660 670 680 690 700
RFDDGIKLDK EGWFSVTPEK IAEHIAGRVS QSFNCDIIVD AFCGVGGNTI
710 720 730 740 750
QFALTGKRVI AIDIDPVKID LARNNAEVYG VADKIEFICG DFLLLAPCLK
760 770 780 790 800
ADVVFLSPPW GGPDYATAET FDIRTMMSPD GFEIFRLSQK ITNNIVYFLP
810 820 830 840 850
RNADVDQVAS LAGPGGQVEI EQNFLNNKLK TITAYFGDLI RRPALRSAEA
Length:850
Mass (Da):96,045
Last modified:April 3, 2007 - v1
Checksum:i1C47E614F90382DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03040398 Genomic DNA No translation available.
RefSeqiNP_001101374.1, NM_001107904.1
UniGeneiRn.48378

Genome annotation databases

EnsembliENSRNOT00000010783; ENSRNOP00000010783; ENSRNOG00000008156
GeneIDi312947
KEGGirno:312947
UCSCiRGD:1309953 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03040398 Genomic DNA No translation available.
RefSeqiNP_001101374.1, NM_001107904.1
UniGeneiRn.48378

3D structure databases

ProteinModelPortaliP85107
SMRiP85107
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010783

PTM databases

iPTMnetiP85107
PhosphoSitePlusiP85107

Proteomic databases

PaxDbiP85107
PRIDEiP85107

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000010783; ENSRNOP00000010783; ENSRNOG00000008156
GeneIDi312947
KEGGirno:312947
UCSCiRGD:1309953 rat

Organism-specific databases

CTDi96764
RGDi1309953 Tgs1

Phylogenomic databases

eggNOGiKOG2730 Eukaryota
ENOG410XRUD LUCA
GeneTreeiENSGT00390000018056
HOGENOMiHOG000154561
HOVERGENiHBG059797
InParanoidiP85107
KOiK14292
OMAiMNTRNKV
OrthoDBiEOG091G0DYO
PhylomeDBiP85107
TreeFamiTF313065

Enzyme and pathway databases

ReactomeiR-RNO-191859 snRNP Assembly
R-RNO-400206 Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha)

Miscellaneous databases

PROiPR:P85107

Gene expression databases

BgeeiENSRNOG00000008156 Expressed in 9 organ(s), highest expression level in skeletal muscle tissue
GenevisibleiP85107 RN

Family and domain databases

InterProiView protein in InterPro
IPR019012 RNA_cap_Gua-N2-MeTrfase
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF09445 Methyltransf_15, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTGS1_RAT
AccessioniPrimary (citable) accession number: P85107
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: April 3, 2007
Last modified: September 12, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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