Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Stromal interaction molecule 1

Gene

Stim1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in mediating store-operated Ca2+ entry (SOCE), a Ca2+ influx following depletion of intracellular Ca2+ stores (By similarity). Acts as Ca2+ sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca2+ depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca2+ release-activated Ca2+ (CRAC) channel subunit ORAI1 (PubMed:16208375). Involved in enamel formation (By similarity). Activated following interaction with STIMATE, leading to promote STIM1 conformational switch (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi76 – 87By similarityAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCalcium transport, Ion transport, Transport
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-5578775 Ion homeostasis
R-RNO-983695 Antigen activates B Cell Receptor (BCR) leading to generation of second messengers

Names & Taxonomyi

Protein namesi
Recommended name:
Stromal interaction molecule 1
Gene namesi
Name:Stim1Imported
Synonyms:SimBy similarity
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1306831 Stim1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 213ExtracellularSequence analysisAdd BLAST191
Transmembranei214 – 234HelicalSequence analysisAdd BLAST21
Topological domaini235 – 685CytoplasmicSequence analysisAdd BLAST451

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Endoplasmic reticulum, Membrane, Microtubule, Sarcoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi76D → A: Increases Ca(2+) influx through activation of CRAC channels, even when Ca(2+) stores are not depleted. 1 Publication1
Mutagenesisi78D → A: Increases Ca(2+) influx through activation of CRAC channels, even when Ca(2+) stores are not depleted. 1 Publication1
Mutagenesisi87E → Q: Increases Ca(2+) influx through activation of CRAC channels, even when Ca(2+) stores are not depleted. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL3832645

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000024827023 – 685Stromal interaction molecule 1Sequence analysisAdd BLAST663

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi131N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi171N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei257PhosphoserineCombined sources1
Modified residuei504PhosphothreonineBy similarity1
Modified residuei512PhosphoserineCombined sources1
Modified residuei517PhosphothreonineBy similarity1
Modified residuei519PhosphoserineCombined sources1
Modified residuei521PhosphoserineCombined sources1
Modified residuei523PhosphoserineBy similarity1
Modified residuei524PhosphoserineBy similarity1
Modified residuei567PhosphoserineCombined sources1
Modified residuei575PhosphoserineCombined sources1
Modified residuei602PhosphoserineBy similarity1
Modified residuei608PhosphoserineBy similarity1
Modified residuei618PhosphoserineBy similarity1
Modified residuei621PhosphoserineBy similarity1
Modified residuei628PhosphoserineBy similarity1
Modified residuei660PhosphoserineCombined sources1
Modified residuei665PhosphothreonineBy similarity1
Modified residuei668PhosphoserineCombined sources1

Post-translational modificationi

Glycosylation is required for cell surface expression.By similarity
Phosphorylated predominantly on Ser residues.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP84903
PRIDEiP84903

PTM databases

iPTMnetiP84903
PhosphoSitePlusiP84903

Expressioni

Gene expression databases

BgeeiENSRNOG00000020425 Expressed in 10 organ(s), highest expression level in skeletal muscle tissue
ExpressionAtlasiP84903 baseline and differential
GenevisibleiP84903 RN

Interactioni

Subunit structurei

Monomer in the presence of Ca2+. It oligomerizes in absence of Ca2+. Forms homooligomers and heterooligomers with STIM2. Interacts (via the transmembrane region and the SOAR/CAD domain) with SPPL3; the interaction promotes the binding of STIM1 to ORAI1. Interacts with ORAI1. Interacts with MAPRE1; probably required for targeting to the growing microtubule plus ends. Interacts with CRACR2A/EFCAB4B; the interaction is direct and takes place in absence of Ca2+. Forms a complex with CRACR2A/EFCAB4B and ORAI1 at low concentration of Ca2+, the complex dissociates at elevated Ca2+ concentrations. Interacts with SARAF, promoting a slow inactivation of STIM1-dependent SOCE activity, possibly by facilitating the deoligomerization of STIM1. Interacts with ASPH. Interacts with SLC35G1; intracellular Ca2+-dependent. May interact with ATP1A1, ATP2A2, ATP2B1, ATP2B4, KPNB1 and XPO1; through SLC35G1. Interacts with TMEM203. Interacts with STIMATE, promoting STIM1 conformational switch (By similarity). Interacts with TMEM178A (By similarity). Interacts with CASQ1 (via C-terminal end and preferentially with the monomeric form); this interaction increases in response to a depletion of intracellular calcium, decreases both STIM1 aggregation and clustering, interaction of STIM1 with ORAI1 and store-operated Ca2+ entry (SOCE) activity (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

CORUMiP84903
IntActiP84903, 1 interactor
STRINGi10116.ENSRNOP00000027685

Structurei

3D structure databases

ProteinModelPortaliP84903
SMRiP84903
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 98EF-handSequence analysisAdd BLAST36
Domaini132 – 190SAMPROSITE-ProRule annotationAdd BLAST59

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni334 – 444SOAR/CADBy similarityAdd BLAST111

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili248 – 442By similarityAdd BLAST195

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi642 – 645Microtubule tip localization signal4

Domaini

The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.By similarity
The STIM1 Orai1-activating region/CRAC-activating domain (SOAR/CAD) mediates interaction with ORAI1 to activate the channel.By similarity

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4403 Eukaryota
ENOG410XRM6 LUCA
GeneTreeiENSGT00390000000214
HOGENOMiHOG000261647
HOVERGENiHBG054652
InParanoidiP84903
KOiK16059
PhylomeDBiP84903
TreeFamiTF313487

Family and domain databases

CDDicd09573 SAM_STIM1, 1 hit
cd11722 SOAR, 1 hit
InterProiView protein in InterPro
IPR001660 SAM
IPR013761 SAM/pointed_sf
IPR032393 SOAR
IPR037608 STIM
IPR037609 STIM1_SAM
IPR030463 STM1
PANTHERiPTHR15136 PTHR15136, 1 hit
PTHR15136:SF9 PTHR15136:SF9, 1 hit
PfamiView protein in Pfam
PF07647 SAM_2, 1 hit
PF16533 SOAR, 1 hit
SMARTiView protein in SMART
SM00454 SAM, 1 hit
SUPFAMiSSF47769 SSF47769, 1 hit
PROSITEiView protein in PROSITE
PS50105 SAM_DOMAIN, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

P84903-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDVCARLALW LLWGLLLHQG QSLSHSHSEK NTGASSGATS EESTEAEFCR
60 70 80 90 100
IDKPLCHSED EKLSFEAVRN IHKLMDDDAN GDVDVEESDE FLREDLNYHD
110 120 130 140 150
PTVKHSTFHG EDKLISVEDL WKAWKASEVY NWTVDEVIQW LITYVELPQY
160 170 180 190 200
EETFRKLQLT GHAMPRLAVT NTTMTGTVLK MTDRSHRQKL QLKALDTVLF
210 220 230 240 250
GPPLLTRHNH LKDFMLVVSI VIGVGGCWFA YIQNRYSKEH MKKMMKDLEG
260 270 280 290 300
LHRAEQSLHD LQERLHKAQE EHRTVEVEKV HLEKKLRDEI NLAKQEAQRL
310 320 330 340 350
KELREGTENE RSRQKYAEEE LEQVREALRK AEKELESHSS WYAPEALQKW
360 370 380 390 400
LQLTHEVEVQ YYNIKKQNAE RQLLVAKEGA EKIKKKRNTL FGTFHVAHSS
410 420 430 440 450
SLDDVDHKIL TAKQALSEVT AALRERLHRW QQIEILCGFQ IVNNPGIHSL
460 470 480 490 500
VAALNIDPSW MGSTRPNPAH FIMTDDVDDM DEEIVSPLSM QSPSLQSSVR
510 520 530 540 550
QRLTEPQHGL GSQRDLTHSD SESSLHTSDR QRVAPKPPQM GRAADEALNA
560 570 580 590 600
TSSNGSHRLI EGVHPGSLVE KLPDSPALAK KTILALNHGL DKAHSLMELN
610 620 630 640 650
PSVPPGGSPL LDSSHSHSPS SPDPDTPSPV GDSRALQGSR NTRIPHLAGK
660 670 680
KAMAEEDNGS IGEETDSSPG RKKFPLKIFK KPLKK
Length:685
Mass (Da):77,449
Last modified:September 5, 2006 - v1
Checksum:iFD2DA28427D2E41E
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2K5C8A0A0G2K5C8_RAT
Stromal interaction molecule 1
Stim1 Stim1_predicted, rCG_39340
716Annotation score:
A0A1B0GWQ1A0A1B0GWQ1_RAT
Stromal interaction molecule 1
Stim1
540Annotation score:
A0A1B0GWK9A0A1B0GWK9_RAT
Stromal interaction molecule 1
Stim1
252Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03000423 Genomic DNA No translation available.
AABR03002698 Genomic DNA No translation available.
RefSeqiNP_001101966.2, NM_001108496.2
UniGeneiRn.106771

Genome annotation databases

EnsembliENSRNOT00000027685; ENSRNOP00000027685; ENSRNOG00000020425
GeneIDi361618
KEGGirno:361618
UCSCiRGD:1306831 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03000423 Genomic DNA No translation available.
AABR03002698 Genomic DNA No translation available.
RefSeqiNP_001101966.2, NM_001108496.2
UniGeneiRn.106771

3D structure databases

ProteinModelPortaliP84903
SMRiP84903
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

CORUMiP84903
IntActiP84903, 1 interactor
STRINGi10116.ENSRNOP00000027685

Chemistry databases

ChEMBLiCHEMBL3832645

PTM databases

iPTMnetiP84903
PhosphoSitePlusiP84903

Proteomic databases

PaxDbiP84903
PRIDEiP84903

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000027685; ENSRNOP00000027685; ENSRNOG00000020425
GeneIDi361618
KEGGirno:361618
UCSCiRGD:1306831 rat

Organism-specific databases

CTDi6786
RGDi1306831 Stim1

Phylogenomic databases

eggNOGiKOG4403 Eukaryota
ENOG410XRM6 LUCA
GeneTreeiENSGT00390000000214
HOGENOMiHOG000261647
HOVERGENiHBG054652
InParanoidiP84903
KOiK16059
PhylomeDBiP84903
TreeFamiTF313487

Enzyme and pathway databases

ReactomeiR-RNO-5578775 Ion homeostasis
R-RNO-983695 Antigen activates B Cell Receptor (BCR) leading to generation of second messengers

Miscellaneous databases

PROiPR:P84903

Gene expression databases

BgeeiENSRNOG00000020425 Expressed in 10 organ(s), highest expression level in skeletal muscle tissue
ExpressionAtlasiP84903 baseline and differential
GenevisibleiP84903 RN

Family and domain databases

CDDicd09573 SAM_STIM1, 1 hit
cd11722 SOAR, 1 hit
InterProiView protein in InterPro
IPR001660 SAM
IPR013761 SAM/pointed_sf
IPR032393 SOAR
IPR037608 STIM
IPR037609 STIM1_SAM
IPR030463 STM1
PANTHERiPTHR15136 PTHR15136, 1 hit
PTHR15136:SF9 PTHR15136:SF9, 1 hit
PfamiView protein in Pfam
PF07647 SAM_2, 1 hit
PF16533 SOAR, 1 hit
SMARTiView protein in SMART
SM00454 SAM, 1 hit
SUPFAMiSSF47769 SSF47769, 1 hit
PROSITEiView protein in PROSITE
PS50105 SAM_DOMAIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSTIM1_RAT
AccessioniPrimary (citable) accession number: P84903
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: September 5, 2006
Last modified: September 12, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again