UniProtKB - P84614 (SCX28_HOTTS)
Protein
Alpha-toxin Bs-Tx28
Gene
N/A
Organism
Hottentotta tamulus sindicus (Scorpion) (Buthus sindicus)
Status
Functioni
Alpha toxins bind voltage-independently at site-3 of sodium channels (Nav) and inhibit the inactivation of the activated channels, thereby blocking neuronal transmission. This toxin inhibits the inactivation of activated TTX-sensitive sodium channels (Nav).1 Publication
GO - Molecular functioni
- ion channel inhibitor activity Source: InterPro
- toxin activity Source: UniProtKB-KW
GO - Biological processi
- defense response Source: InterPro
Keywordsi
Molecular function | Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha-toxin Bs-Tx28 |
Organismi | Hottentotta tamulus sindicus (Scorpion) (Buthus sindicus) |
Taxonomic identifieri | 42519 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Mesobuthus › |
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPathology & Biotechi
Toxic dosei
LD50 is 0.088 µg/g by subcutaneous injection into mice, 0.0023 µg/g by intracerebroventricular injection into mice and 14.3 µg/g in B.germanica.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000066747 | 1 – 65 | Alpha-toxin Bs-Tx28Add BLAST | 65 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 13 ↔ 64 | PROSITE-ProRule annotation | ||
Disulfide bondi | 17 ↔ 37 | PROSITE-ProRule annotation | ||
Disulfide bondi | 23 ↔ 47 | PROSITE-ProRule annotation | ||
Disulfide bondi | 27 ↔ 49 | PROSITE-ProRule annotation | ||
Modified residuei | 65 | Arginine amide1 Publication | 1 |
Keywords - PTMi
Amidation, Disulfide bondProteomic databases
PRIDEi | P84614 |
Expressioni
Tissue specificityi
Expressed by the venom gland.1 Publication
Structurei
3D structure databases
ProteinModelPortali | P84614 |
SMRi | P84614 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 65 | LCN-type CS-alpha/betaPROSITE-ProRule annotationAdd BLAST | 63 |
Domaini
Has the structural arrangement of an alpha-helix connected to antiparallel beta-sheets by disulfide bonds (beta/alpha/beta/beta).Curated
Sequence similaritiesi
Belongs to the long (4 C-C) scorpion toxin superfamily. Sodium channel inhibitor family. Alpha subfamily.Curated
Family and domain databases
CDDi | cd00107 Knot1, 1 hit |
Gene3Di | 3.30.30.10, 1 hit |
InterProi | View protein in InterPro IPR003614 Scorpion_toxin-like IPR036574 Scorpion_toxin-like_sf IPR018218 Scorpion_toxinL IPR002061 Scorpion_toxinL/defensin |
Pfami | View protein in Pfam PF00537 Toxin_3, 1 hit |
PRINTSi | PR00285 SCORPNTOXIN |
SMARTi | View protein in SMART SM00505 Knot1, 1 hit |
SUPFAMi | SSF57095 SSF57095, 1 hit |
PROSITEi | View protein in PROSITE PS51863 LCN_CSAB, 1 hit |
i Sequence
Sequence statusi: Complete.
P84614-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
GVRDAYIADD KNCVYTCGSN SYCNTECTKN GAESGYCQWF GRWGNGCWCI
60
KLPDKVPIRI PGKCR
Mass spectrometryi
Similar proteinsi
Cross-referencesi
3D structure databases
ProteinModelPortali | P84614 |
SMRi | P84614 |
ModBasei | Search... |
MobiDBi | Search... |
Proteomic databases
PRIDEi | P84614 |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Family and domain databases
CDDi | cd00107 Knot1, 1 hit |
Gene3Di | 3.30.30.10, 1 hit |
InterProi | View protein in InterPro IPR003614 Scorpion_toxin-like IPR036574 Scorpion_toxin-like_sf IPR018218 Scorpion_toxinL IPR002061 Scorpion_toxinL/defensin |
Pfami | View protein in Pfam PF00537 Toxin_3, 1 hit |
PRINTSi | PR00285 SCORPNTOXIN |
SMARTi | View protein in SMART SM00505 Knot1, 1 hit |
SUPFAMi | SSF57095 SSF57095, 1 hit |
PROSITEi | View protein in PROSITE PS51863 LCN_CSAB, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | SCX28_HOTTS | |
Accessioni | P84614Primary (citable) accession number: P84614 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 24, 2006 |
Last sequence update: | January 24, 2006 | |
Last modified: | December 5, 2018 | |
This is version 47 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Animal Toxin Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- SIMILARITY comments
Index of protein domains and families