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Protein

Cationic peroxidase SPC4

Gene

Sb03g046810

Organism
Sorghum bicolor (Sorghum) (Sorghum vulgare)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Removal of H2O2, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue. Has a high preference for hydroxycinnamates as substrates. Substrate preference is ferulic acid > p-coumaric acid > N-acetyl tyrosine methyl ester > N-acetyl-tyrosine > tyrosine > catechol > Gly-Tyr-Gly. May be involved in the formation of diferulate linkages in the plant cell wall.PROSITE-ProRule annotation1 Publication

Catalytic activityi

2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.1 Publication

Cofactori

Protein has several cofactor binding sites:

pH dependencei

Optimum pH is 3.8 with 2.2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, 5.5 with ferulic acid as substrate, and 6.5 with N-acetyl-L-tyrosine as substrate.

Temperature dependencei

Loss of activity at temperature above 55 degrees Celsius. In the presence of excess calcium, full activity is kept at 65 degrees Celsius for 90 minutes.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei77Transition state stabilizerPROSITE-ProRule annotationBy similarity1
Active sitei81Proton acceptorPROSITE-ProRule annotationBy similarity1
Metal bindingi82Calcium 1PROSITE-ProRule annotationBy similarity1
Metal bindingi85Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi87Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi89Calcium 1PROSITE-ProRule annotationBy similarity1
Metal bindingi91Calcium 1PROSITE-ProRule annotationBy similarity1
Binding sitei181Substrate; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi211Iron (heme axial ligand)PROSITE-ProRule annotationBy similarity1
Metal bindingi212Calcium 2PROSITE-ProRule annotationBy similarity1
Metal bindingi257Calcium 2PROSITE-ProRule annotation1
Metal bindingi260Calcium 2PROSITE-ProRule annotation1
Metal bindingi265Calcium 2PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase, Peroxidase
Biological processHydrogen peroxide
LigandCalcium, Heme, Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.11.1.7 5768

Protein family/group databases

PeroxiBasei853 SbPrx50

Names & Taxonomyi

Protein namesi
Recommended name:
Cationic peroxidase SPC4 (EC:1.11.1.7)
Gene namesi
Ordered Locus Names:Sb03g046810
OrganismiSorghum bicolor (Sorghum) (Sorghum vulgare)
Taxonomic identifieri4558 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeSorghinaeSorghum
Proteomesi
  • UP000000768 Componentsi: Chromosome 3, Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 311 PublicationAdd BLAST31
ChainiPRO_000004585332 – 362Cationic peroxidase SPC4Add BLAST331

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi83 ↔ 88PROSITE-ProRule annotationBy similarity
Glycosylationi109N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi138 ↔ 333PROSITE-ProRule annotation
Disulfide bondi218 ↔ 245PROSITE-ProRule annotation
Glycosylationi234N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi332N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

The proportions of glycoforms I and II are 35% and 65% respectively.

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Present in germinated and ungerminated grain, seedlings, and leaves and stem of the mature plant.1 Publication

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi4558.Sb03g046810.1

Structurei

Secondary structure

1362
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SMRiP84516
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IHDZ Eukaryota
ENOG410ZMT4 LUCA
HOGENOMiHOG000237557
InParanoidiP84516
KOiK00430
OMAiFTVAMIK
OrthoDBiEOG09360F5G

Family and domain databases

CDDicd00693 secretory_peroxidase, 1 hit
InterProiView protein in InterPro
IPR010255 Haem_peroxidase
IPR002016 Haem_peroxidase_pln/fun/bac
IPR000823 Peroxidase_pln
IPR019794 Peroxidases_AS
IPR019793 Peroxidases_heam-ligand_BS
IPR033905 Secretory_peroxidase
PfamiView protein in Pfam
PF00141 peroxidase, 1 hit
PRINTSiPR00458 PEROXIDASE
PR00461 PLPEROXIDASE
SUPFAMiSSF48113 SSF48113, 1 hit
PROSITEiView protein in PROSITE
PS00435 PEROXIDASE_1, 1 hit
PS00436 PEROXIDASE_2, 1 hit
PS50873 PEROXIDASE_4, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P84516-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSRAPTLAAA AAVAAVVLIC SSSTATAADG NARQPPLAPG LSFDFYKRSC
60 70 80 90 100
PKAESIVRSF VQDAVRRDVG LAAGLLRLHF HDCFVQGCDA SVLLDGSATG
110 120 130 140 150
PGEQQAPPNL TLRPTAFKAI NDIHDRLHKE CGGTVVSCSD VLALAARDSV
160 170 180 190 200
VVSGGPSYKV PLGRRDSASF ATQQDVLSGL PPPTAAVPAL LAVLSKINLD
210 220 230 240 250
ATDLVALSGG HTIGLGHCTS FEDRLFPRPD PTLNATFAGQ LRRTCPAKGT
260 270 280 290 300
DRRTPLDVRT PNAFDNKYYV NLVNREGLFT SDQDLFSNAR TRALVDKFAR
310 320 330 340 350
SQRDFFDQFA FSVVKMGQIK VLTGTQGQIR TNCSARNAAG TTMLPWSVSV
360
VEEAADESLG VF
Length:362
Mass (Da):38,452
Last modified:February 22, 2012 - v2
Checksum:i13AB5B89D77CC6E5
GO

Mass spectrometryi

Molecular mass is 33246±50 Da from positions 31 - 362. Determined by MALDI. Deglycosylated form.1 Publication
Molecular mass is 34283±50 Da from positions 31 - 362. Determined by MALDI. Glycoform I.1 Publication
Molecular mass is 35631±50 Da from positions 31 - 362. Determined by MALDI. Glycoform II.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM000762 Genomic DNA Translation: EES02106.1
CD222694 mRNA No translation available.
CD226279 mRNA No translation available.
RefSeqiXP_002456986.1, XM_002456941.1

Genome annotation databases

EnsemblPlantsiEES02106; EES02106; SORBI_3003G437400
GeneIDi8074114
GrameneiEES02106; EES02106; SORBI_3003G437400
KEGGisbi:8074114

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM000762 Genomic DNA Translation: EES02106.1
CD222694 mRNA No translation available.
CD226279 mRNA No translation available.
RefSeqiXP_002456986.1, XM_002456941.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5AOGX-ray1.27A34-340[»]
SMRiP84516
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4558.Sb03g046810.1

Protein family/group databases

PeroxiBasei853 SbPrx50

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiEES02106; EES02106; SORBI_3003G437400
GeneIDi8074114
GrameneiEES02106; EES02106; SORBI_3003G437400
KEGGisbi:8074114

Phylogenomic databases

eggNOGiENOG410IHDZ Eukaryota
ENOG410ZMT4 LUCA
HOGENOMiHOG000237557
InParanoidiP84516
KOiK00430
OMAiFTVAMIK
OrthoDBiEOG09360F5G

Enzyme and pathway databases

BRENDAi1.11.1.7 5768

Family and domain databases

CDDicd00693 secretory_peroxidase, 1 hit
InterProiView protein in InterPro
IPR010255 Haem_peroxidase
IPR002016 Haem_peroxidase_pln/fun/bac
IPR000823 Peroxidase_pln
IPR019794 Peroxidases_AS
IPR019793 Peroxidases_heam-ligand_BS
IPR033905 Secretory_peroxidase
PfamiView protein in Pfam
PF00141 peroxidase, 1 hit
PRINTSiPR00458 PEROXIDASE
PR00461 PLPEROXIDASE
SUPFAMiSSF48113 SSF48113, 1 hit
PROSITEiView protein in PROSITE
PS00435 PEROXIDASE_1, 1 hit
PS00436 PEROXIDASE_2, 1 hit
PS50873 PEROXIDASE_4, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPER1_SORBI
AccessioniPrimary (citable) accession number: P84516
Secondary accession number(s): C5XIP7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: February 22, 2012
Last modified: March 28, 2018
This is version 75 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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