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Protein

Serine/arginine-rich splicing factor 3

Gene

Srsf3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Splicing factor that specifically promotes exon-inclusion during alternative splicing. Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing. Also functions as export adapter involved in mRNA nuclear export. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity. Involved in nuclear export of m6A-containing mRNAs via interaction with YTHDC1: interaction with YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export. RNA-binding is semi-sequence specific.By similarity

GO - Molecular functioni

  • phospholipase binding Source: BHF-UCL
  • RNA binding Source: UniProtKB
  • sequence-specific mRNA binding Source: MGI

GO - Biological processi

  • cellular response to leukemia inhibitory factor Source: MGI
  • insulin receptor signaling pathway Source: BHF-UCL
  • mRNA cis splicing, via spliceosome Source: GO_Central
  • mRNA export from nucleus Source: UniProtKB
  • regulation of alternative mRNA splicing, via spliceosome Source: GO_Central
  • regulation of mRNA splicing, via spliceosome Source: UniProtKB
  • RNA splicing Source: BHF-UCL

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, mRNA splicing, mRNA transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-109688 Cleavage of Growing Transcript in the Termination Region
R-MMU-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript
R-MMU-72163 mRNA Splicing - Major Pathway
R-MMU-72187 mRNA 3'-end processing

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/arginine-rich splicing factor 3
Alternative name(s):
Pre-mRNA-splicing factor SRP20
Protein X16
Splicing factor, arginine/serine-rich 3
Gene namesi
Name:Srsf3
Synonyms:Sfrs3, Srp20, X16
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:98285 Srsf3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000819241 – 164Serine/arginine-rich splicing factor 3Add BLAST164

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei5PhosphoserineBy similarity1
Modified residuei23N6-acetyllysineBy similarity1

Post-translational modificationi

Phosphorylated by CLK1, CLK2, CLK3 and CLK4. Extensively phosphorylated on serine residues in the RS domain.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP84104
PaxDbiP84104
PeptideAtlasiP84104
PRIDEiP84104

PTM databases

iPTMnetiP84104
PhosphoSitePlusiP84104

Expressioni

Tissue specificityi

Highest expression in thymus and pre-B cell lines; high, in testis, brain and spleen; very low in heart and not detectable in liver and kidney.1 Publication

Inductioni

Isoform long is induced by serum; in a tissue culture.1 Publication

Gene expression databases

BgeeiENSMUSG00000071172 Expressed in 295 organ(s), highest expression level in telencephalon
CleanExiMM_SFRS3
ExpressionAtlasiP84104 baseline and differential
GenevisibleiP84104 MM

Interactioni

Subunit structurei

Interacts with CPSF6. Interacts with RBMY1A1. Interacts with SREK1/SFRS12. Interacts with NXF1. Interacts with YTHDC1, leading to recruitment to RNA elements adjacent to m6A sites.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203189, 10 interactors
IntActiP84104, 5 interactors
MINTiP84104
STRINGi10090.ENSMUSP00000117045

Structurei

3D structure databases

ProteinModelPortaliP84104
SMRiP84104
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 83RRMPROSITE-ProRule annotationAdd BLAST74
Repeati119 – 133B-1Add BLAST15
Repeati149 – 164B-2Add BLAST16

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 90Sufficient for interaction with NXF1By similarityAdd BLAST90
Regioni119 – 1642 X approximate repeats, basicAdd BLAST46

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi86 – 164Arg/Ser-rich (RS domain)Add BLAST79

Sequence similaritiesi

Belongs to the splicing factor SR family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0107 Eukaryota
ENOG4111N8Y LUCA
GeneTreeiENSGT00910000144115
HOVERGENiHBG107480
InParanoidiP84104
KOiK12892
OMAiKHICSTI
OrthoDBiEOG091G17YQ
PhylomeDBiP84104
TreeFamiTF351858

Family and domain databases

Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform Long (identifier: P84104-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MHRDSCPLDC KVYVGNLGNN GNKTELERAF GYYGPLRSVW VARNPPGFAF
60 70 80 90 100
VEFEDPRDAA DAVRELDGRT LCGCRVRVEL SNGEKRSRNR GPPPSWGRRP
110 120 130 140 150
RDDYRRRSPP PRRRSPRRRS FSRSRSRSLS RDRRRERSLS RERNHKPSRS
160
FSRSRSRSRS NERK
Length:164
Mass (Da):19,330
Last modified:August 16, 2004 - v1
Checksum:i02F0A5EE33FF28A0
GO
Isoform Short (identifier: P84104-2) [UniParc] [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     115-124: SPRRRSFSRS → VTIMSLLTTL
     125-164: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:124
Mass (Da):14,203
Checksum:i78979851C38232C1
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005861115 – 124SPRRRSFSRS → VTIMSLLTTL in isoform Short. Curated10
Alternative sequenceiVSP_005862125 – 164Missing in isoform Short. CuratedAdd BLAST40

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53824 mRNA Translation: CAA37821.1
X91656 Genomic DNA Translation: CAA62844.1
X91656 Genomic DNA Translation: CAA62845.1
AK011657 mRNA Translation: BAB27762.1
AK078891 mRNA Translation: BAC37445.1
AK134177 mRNA Translation: BAE22040.1
AK135298 mRNA Translation: BAE22483.1
AK145089 mRNA Translation: BAE26230.1
AK145715 mRNA Translation: BAE26606.1
AK146010 mRNA Translation: BAE26830.1
AK146253 mRNA Translation: BAE27015.1
AK150912 mRNA Translation: BAE29950.1
AK153209 mRNA Translation: BAE31808.1
BC068111 mRNA Translation: AAH68111.1
BC071196 mRNA Translation: AAH71196.1
BC083316 mRNA Translation: AAH83316.1
CCDSiCCDS28590.1
PIRiS14016
RefSeqiNP_038691.1, NM_013663.5 [P84104-1]
UniGeneiMm.473125
Mm.486331
Mm.6787

Genome annotation databases

EnsembliENSMUST00000037776; ENSMUSP00000049025; ENSMUSG00000071172 [P84104-2]
ENSMUST00000130216; ENSMUSP00000117045; ENSMUSG00000071172 [P84104-1]
GeneIDi20383
KEGGimmu:20383
UCSCiuc008bsd.2 mouse

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53824 mRNA Translation: CAA37821.1
X91656 Genomic DNA Translation: CAA62844.1
X91656 Genomic DNA Translation: CAA62845.1
AK011657 mRNA Translation: BAB27762.1
AK078891 mRNA Translation: BAC37445.1
AK134177 mRNA Translation: BAE22040.1
AK135298 mRNA Translation: BAE22483.1
AK145089 mRNA Translation: BAE26230.1
AK145715 mRNA Translation: BAE26606.1
AK146010 mRNA Translation: BAE26830.1
AK146253 mRNA Translation: BAE27015.1
AK150912 mRNA Translation: BAE29950.1
AK153209 mRNA Translation: BAE31808.1
BC068111 mRNA Translation: AAH68111.1
BC071196 mRNA Translation: AAH71196.1
BC083316 mRNA Translation: AAH83316.1
CCDSiCCDS28590.1
PIRiS14016
RefSeqiNP_038691.1, NM_013663.5 [P84104-1]
UniGeneiMm.473125
Mm.486331
Mm.6787

3D structure databases

ProteinModelPortaliP84104
SMRiP84104
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203189, 10 interactors
IntActiP84104, 5 interactors
MINTiP84104
STRINGi10090.ENSMUSP00000117045

PTM databases

iPTMnetiP84104
PhosphoSitePlusiP84104

Proteomic databases

MaxQBiP84104
PaxDbiP84104
PeptideAtlasiP84104
PRIDEiP84104

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037776; ENSMUSP00000049025; ENSMUSG00000071172 [P84104-2]
ENSMUST00000130216; ENSMUSP00000117045; ENSMUSG00000071172 [P84104-1]
GeneIDi20383
KEGGimmu:20383
UCSCiuc008bsd.2 mouse

Organism-specific databases

CTDi6428
MGIiMGI:98285 Srsf3

Phylogenomic databases

eggNOGiKOG0107 Eukaryota
ENOG4111N8Y LUCA
GeneTreeiENSGT00910000144115
HOVERGENiHBG107480
InParanoidiP84104
KOiK12892
OMAiKHICSTI
OrthoDBiEOG091G17YQ
PhylomeDBiP84104
TreeFamiTF351858

Enzyme and pathway databases

ReactomeiR-MMU-109688 Cleavage of Growing Transcript in the Termination Region
R-MMU-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript
R-MMU-72163 mRNA Splicing - Major Pathway
R-MMU-72187 mRNA 3'-end processing

Miscellaneous databases

ChiTaRSiSrsf3 mouse
PROiPR:P84104
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000071172 Expressed in 295 organ(s), highest expression level in telencephalon
CleanExiMM_SFRS3
ExpressionAtlasiP84104 baseline and differential
GenevisibleiP84104 MM

Family and domain databases

Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSRSF3_MOUSE
AccessioniPrimary (citable) accession number: P84104
Secondary accession number(s): O08831, P23152, Q564E9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: November 7, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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