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Protein

Serine/arginine-rich splicing factor 3

Gene

SRSF3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Splicing factor that specifically promotes exon-inclusion during alternative splicing (PubMed:26876937). Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing (PubMed:26876937). Also functions as export adapter involved in mRNA nuclear export (PubMed:11336712, PubMed:18364396, PubMed:28984244). Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity (PubMed:11336712, PubMed:18364396). Involved in nuclear export of m6A-containing mRNAs via interaction with YTHDC1: interaction with YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed:28984244). RNA-binding is semi-sequence specific (PubMed:17036044).5 Publications

GO - Molecular functioni

  • RNA binding Source: UniProtKB
  • sequence-specific mRNA binding Source: Ensembl

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, mRNA splicing, mRNA transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-109688 Cleavage of Growing Transcript in the Termination Region
R-HSA-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72187 mRNA 3'-end processing
SIGNORiP84103

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/arginine-rich splicing factor 3
Alternative name(s):
Pre-mRNA-splicing factor SRP20
Splicing factor, arginine/serine-rich 3
Gene namesi
Name:SRSF3
Synonyms:SFRS3, SRP20
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000112081.16
HGNCiHGNC:10785 SRSF3
MIMi603364 gene
neXtProtiNX_P84103

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi86R → E: Abolishes interaction with NXF1. 1 Publication1
Mutagenesisi88R → E: Abolishes interaction with NXF1. 1 Publication1
Mutagenesisi90R → E: Abolishes interaction with NXF1. 1 Publication1

Organism-specific databases

DisGeNETi6428
OpenTargetsiENSG00000112081
PharmGKBiPA35701

Polymorphism and mutation databases

BioMutaiSRSF3
DMDMi51338672

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000819231 – 164Serine/arginine-rich splicing factor 3Add BLAST164

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei5PhosphoserineCombined sources1
Modified residuei23N6-acetyllysineCombined sources1

Post-translational modificationi

Phosphorylated by CLK1, CLK2, CLK3 and CLK4. Extensively phosphorylated on serine residues in the RS domain.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP84103
MaxQBiP84103
PaxDbiP84103
PeptideAtlasiP84103
PRIDEiP84103
ProteomicsDBi57753
TopDownProteomicsiP84103-1 [P84103-1]

PTM databases

iPTMnetiP84103
PhosphoSitePlusiP84103
SwissPalmiP84103

Expressioni

Gene expression databases

BgeeiENSG00000112081 Expressed in 238 organ(s), highest expression level in testis
CleanExiHS_SFRS3
ExpressionAtlasiP84103 baseline and differential
GenevisibleiP84103 HS

Organism-specific databases

HPAiCAB012986
HPA056981

Interactioni

Subunit structurei

Interacts with CPSF6 (PubMed:15169763). Interacts with RBMY1A1 (PubMed:15595951). Interacts with SREK1/SFRS12 (PubMed:10757789). Interacts with NXF1 (PubMed:12667464, PubMed:17036044). Interacts with YTHDC1, leading to recruitment to RNA elements adjacent to m6A sites (PubMed:26876937, PubMed:28984244).7 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi112326, 137 interactors
CORUMiP84103
DIPiDIP-32961N
IntActiP84103, 63 interactors
MINTiP84103
STRINGi9606.ENSP00000362820

Structurei

Secondary structure

1164
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP84103
SMRiP84103
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP84103

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 83RRMPROSITE-ProRule annotationAdd BLAST74
Repeati119 – 133B-1Add BLAST15
Repeati149 – 164B-2Add BLAST16

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 90Sufficient for interaction with NXF1Add BLAST90
Regioni119 – 1642 X approximate repeats, basicAdd BLAST46

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi86 – 164Arg/Ser-rich (RS domain)Add BLAST79

Sequence similaritiesi

Belongs to the splicing factor SR family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0107 Eukaryota
ENOG4111N8Y LUCA
GeneTreeiENSGT00910000144115
HOGENOMiHOG000208722
HOVERGENiHBG107480
InParanoidiP84103
KOiK12892
OMAiKHICSTI
OrthoDBiEOG091G17YQ
PhylomeDBiP84103
TreeFamiTF351858

Family and domain databases

Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: P84103-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MHRDSCPLDC KVYVGNLGNN GNKTELERAF GYYGPLRSVW VARNPPGFAF
60 70 80 90 100
VEFEDPRDAA DAVRELDGRT LCGCRVRVEL SNGEKRSRNR GPPPSWGRRP
110 120 130 140 150
RDDYRRRSPP PRRRSPRRRS FSRSRSRSLS RDRRRERSLS RERNHKPSRS
160
FSRSRSRSRS NERK
Length:164
Mass (Da):19,330
Last modified:August 16, 2004 - v1
Checksum:i02F0A5EE33FF28A0
GO
Isoform 2 (identifier: P84103-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     115-164: SPRRRSFSRSRSRSLSRDRRRERSLSRERNHKPSRSFSRSRSRSRSNERK → VTIMSLLTTL

Note: No experimental confirmation available.
Show »
Length:124
Mass (Da):14,203
Checksum:i78979851C38232C1
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087X2D0A0A087X2D0_HUMAN
Serine/arginine-rich-splicing facto...
SRSF3
95Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056243115 – 164SPRRR…SNERK → VTIMSLLTTL in isoform 2. 1 PublicationAdd BLAST50

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10838 mRNA Translation: AAA36648.1
AF107405 mRNA Translation: AAD44523.1
AK304097 mRNA Translation: BAG65003.1
BT007017 mRNA Translation: AAP35663.1
Z85986 Genomic DNA No translation available.
CH471081 Genomic DNA Translation: EAX03898.1
BC000914 mRNA Translation: AAH00914.1
BC069018 mRNA Translation: AAH69018.1
CCDSiCCDS4823.1 [P84103-1]
PIRiI54089
RefSeqiNP_003008.1, NM_003017.4 [P84103-1]
UniGeneiHs.405144

Genome annotation databases

EnsembliENST00000373715; ENSP00000362820; ENSG00000112081 [P84103-1]
ENST00000477442; ENSP00000436036; ENSG00000112081 [P84103-2]
ENST00000620941; ENSP00000482833; ENSG00000112081 [P84103-1]
GeneIDi6428
KEGGihsa:6428
UCSCiuc003omj.4 human [P84103-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10838 mRNA Translation: AAA36648.1
AF107405 mRNA Translation: AAD44523.1
AK304097 mRNA Translation: BAG65003.1
BT007017 mRNA Translation: AAP35663.1
Z85986 Genomic DNA No translation available.
CH471081 Genomic DNA Translation: EAX03898.1
BC000914 mRNA Translation: AAH00914.1
BC069018 mRNA Translation: AAH69018.1
CCDSiCCDS4823.1 [P84103-1]
PIRiI54089
RefSeqiNP_003008.1, NM_003017.4 [P84103-1]
UniGeneiHs.405144

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I2YNMR-A1-86[»]
2I38NMR-A1-86[»]
ProteinModelPortaliP84103
SMRiP84103
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112326, 137 interactors
CORUMiP84103
DIPiDIP-32961N
IntActiP84103, 63 interactors
MINTiP84103
STRINGi9606.ENSP00000362820

PTM databases

iPTMnetiP84103
PhosphoSitePlusiP84103
SwissPalmiP84103

Polymorphism and mutation databases

BioMutaiSRSF3
DMDMi51338672

Proteomic databases

EPDiP84103
MaxQBiP84103
PaxDbiP84103
PeptideAtlasiP84103
PRIDEiP84103
ProteomicsDBi57753
TopDownProteomicsiP84103-1 [P84103-1]

Protocols and materials databases

DNASUi6428
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000373715; ENSP00000362820; ENSG00000112081 [P84103-1]
ENST00000477442; ENSP00000436036; ENSG00000112081 [P84103-2]
ENST00000620941; ENSP00000482833; ENSG00000112081 [P84103-1]
GeneIDi6428
KEGGihsa:6428
UCSCiuc003omj.4 human [P84103-1]

Organism-specific databases

CTDi6428
DisGeNETi6428
EuPathDBiHostDB:ENSG00000112081.16
GeneCardsiSRSF3
HGNCiHGNC:10785 SRSF3
HPAiCAB012986
HPA056981
MIMi603364 gene
neXtProtiNX_P84103
OpenTargetsiENSG00000112081
PharmGKBiPA35701
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0107 Eukaryota
ENOG4111N8Y LUCA
GeneTreeiENSGT00910000144115
HOGENOMiHOG000208722
HOVERGENiHBG107480
InParanoidiP84103
KOiK12892
OMAiKHICSTI
OrthoDBiEOG091G17YQ
PhylomeDBiP84103
TreeFamiTF351858

Enzyme and pathway databases

ReactomeiR-HSA-109688 Cleavage of Growing Transcript in the Termination Region
R-HSA-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72187 mRNA 3'-end processing
SIGNORiP84103

Miscellaneous databases

ChiTaRSiSRSF3 human
EvolutionaryTraceiP84103
GeneWikiiSFRS3
GenomeRNAii6428
PROiPR:P84103
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000112081 Expressed in 238 organ(s), highest expression level in testis
CleanExiHS_SFRS3
ExpressionAtlasiP84103 baseline and differential
GenevisibleiP84103 HS

Family and domain databases

Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSRSF3_HUMAN
AccessioniPrimary (citable) accession number: P84103
Secondary accession number(s): B4E241
, O08831, P23152, Q5R3K0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: November 7, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
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