UniProtKB - P84091 (AP2M1_MOUSE)
AP-2 complex subunit mu
Ap2m1
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 341 | Phosphatidylinositol lipid headgroupBy similarity | 1 | |
Binding sitei | 343 | Phosphatidylinositol lipid headgroupBy similarity | 1 | |
Binding sitei | 345 | Phosphatidylinositol lipid headgroupBy similarity | 1 | |
Binding sitei | 354 | Phosphatidylinositol lipid headgroupBy similarity | 1 | |
Binding sitei | 356 | Phosphatidylinositol lipid headgroupBy similarity | 1 |
GO - Molecular functioni
- clathrin adaptor activity Source: GO_Central
- disordered domain specific binding Source: CAFA
- ion channel binding Source: MGI
- lipid binding Source: UniProtKB-KW
- low-density lipoprotein particle receptor binding Source: MGI
- signal sequence binding Source: MGI
GO - Biological processi
- clathrin-dependent endocytosis Source: UniProtKB
- endocytosis Source: GO_Central
- intracellular protein transport Source: MGI
- negative regulation of protein localization to plasma membrane Source: MGI
- positive regulation of receptor internalization Source: MGI
- positive regulation of synaptic vesicle endocytosis Source: MGI
- postsynaptic neurotransmitter receptor internalization Source: SynGO
- protein-containing complex assembly Source: MGI
- receptor internalization Source: MGI
- regulation of vesicle size Source: MGI
- synaptic vesicle endocytosis Source: MGI
- vesicle budding from membrane Source: MGI
- vesicle-mediated transport Source: GO_Central
Keywordsi
Biological process | Endocytosis, Protein transport, Transport |
Ligand | Lipid-binding |
Enzyme and pathway databases
Reactomei | R-MMU-177504, Retrograde neurotrophin signalling R-MMU-190873, Gap junction degradation R-MMU-196025, Formation of annular gap junctions R-MMU-2132295, MHC class II antigen presentation R-MMU-416993, Trafficking of GluR2-containing AMPA receptors R-MMU-437239, Recycling pathway of L1 R-MMU-5099900, WNT5A-dependent internalization of FZD4 R-MMU-5140745, WNT5A-dependent internalization of FZD2, FZD5 and ROR2 R-MMU-8856825, Cargo recognition for clathrin-mediated endocytosis R-MMU-8856828, Clathrin-mediated endocytosis R-MMU-8866427, VLDLR internalisation and degradation R-MMU-8964038, LDL clearance |
Names & Taxonomyi
Protein namesi | Recommended name: AP-2 complex subunit muAlternative name(s): AP-2 mu chain Adaptor protein complex AP-2 subunit mu Adaptor-related protein complex 2 subunit mu Clathrin assembly protein complex 2 mu medium chain Clathrin coat assembly protein AP50 Clathrin coat-associated protein AP50 Mu2-adaptin Plasma membrane adaptor AP-2 50 kDa protein |
Gene namesi | Name:Ap2m1 Synonyms:Clapm1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1298405, Ap2m1 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity
Other locations
- coated pit By similarity; Peripheral membrane protein ; Cytoplasmic side
Note: AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.1 Publication
Mitochondrion
- mitochondrion Source: MGI
Plasma Membrane
- AP-2 adaptor complex Source: UniProtKB
- clathrin-coated pit Source: MGI
- plasma membrane Source: MGI
Other locations
- cytoplasmic vesicle Source: GO_Central
- glutamatergic synapse Source: SynGO
- intracellular membrane-bounded organelle Source: GO_Central
- postsynapse Source: GOC
- presynapse Source: GOC
- secretory granule Source: MGI
Keywords - Cellular componenti
Cell membrane, Coated pit, MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000193775 | 1 – 435 | AP-2 complex subunit muAdd BLAST | 435 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 45 | PhosphoserineBy similarity | 1 | |
Modified residuei | 156 | PhosphothreonineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P84091 |
jPOSTi | P84091 |
PaxDbi | P84091 |
PeptideAtlasi | P84091 |
PRIDEi | P84091 |
PTM databases
iPTMneti | P84091 |
PhosphoSitePlusi | P84091 |
SwissPalmi | P84091 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000022841, Expressed in retinal neural layer and 115 other tissues |
ExpressionAtlasi | P84091, baseline and differential |
Genevisiblei | P84091, MM |
Interactioni
Subunit structurei
Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1) and a small adaptin (sigma-type subunit AP2S1) (By similarity).
Interacts with ATP6V1H and MEGF10 (By similarity).
Interacts with EGFR and TTGN1 (By similarity).
Interacts with F2R (By similarity).
Interacts with PIP5K1C isoform 1; tyrosine phosphorylation of PIP5K1C weakens the interaction. Does not interact with PIP5K1C isoform 3 (PubMed:16707488).
Interacts with KIAA0319; required for clathrin-mediated endocytosis of KIAA0319 (By similarity).
Interacts with DVL2 (via DEP domain) (By similarity).
Interacts with KCNQ1; mediates estrogen-induced internalization via clathrin-coated vesicles (By similarity).
Interacts with P2RX4 (via internalization motif) (By similarity). Together with AP2A1 or AP2A2 and AP2B1, it interacts with ADAM10; this interaction facilitates ADAM10 endocytosis from the plasma membrane during long-term potentiation in hippocampal neurons (PubMed:23676497).
By similarity2 PublicationsGO - Molecular functioni
- clathrin adaptor activity Source: GO_Central
- disordered domain specific binding Source: CAFA
- ion channel binding Source: MGI
- low-density lipoprotein particle receptor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 198131, 33 interactors |
ComplexPortali | CPX-5152, AP-2 Adaptor complex, alpha1 variant CPX-5153, AP-2 Adaptor complex, alpha2 variant |
IntActi | P84091, 10 interactors |
MINTi | P84091 |
STRINGi | 10090.ENSMUSP00000007216 |
Miscellaneous databases
RNActi | P84091, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P84091 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 170 – 434 | MHDPROSITE-ProRule annotationAdd BLAST | 265 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0938, Eukaryota |
GeneTreei | ENSGT00940000159223 |
InParanoidi | P84091 |
OMAi | ALVFEFC |
OrthoDBi | 725236at2759 |
PhylomeDBi | P84091 |
TreeFami | TF300722 |
Family and domain databases
CDDi | cd09251, AP-2_Mu2_Cterm, 1 hit cd14836, AP2_Mu_N, 1 hit |
InterProi | View protein in InterPro IPR036168, AP2_Mu_C_sf IPR043532, AP2_Mu_N IPR022775, AP_mu_sigma_su IPR001392, Clathrin_mu IPR018240, Clathrin_mu_CS IPR011012, Longin-like_dom_sf IPR028565, MHD IPR043512, Mu2_C |
Pfami | View protein in Pfam PF00928, Adap_comp_sub, 1 hit PF01217, Clat_adaptor_s, 1 hit |
PIRSFi | PIRSF005992, Clathrin_mu, 1 hit |
PRINTSi | PR00314, CLATHRINADPT |
SUPFAMi | SSF49447, SSF49447, 1 hit SSF64356, SSF64356, 1 hit |
PROSITEi | View protein in PROSITE PS00990, CLAT_ADAPTOR_M_1, 1 hit PS00991, CLAT_ADAPTOR_M_2, 1 hit PS51072, MHD, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MIGGLFIYNH KGEVLISRVY RDDIGRNAVD AFRVNVIHAR QQVRSPVTNI
60 70 80 90 100
ARTSFFHVKR SNIWLAAVTK QNVNAAMVFE FLYKMCDVMA AYFGKISEEN
110 120 130 140 150
IKNNFVLIYE LLDEILDFGY PQNSETGALK TFITQQGIKS QHQTKEEQSQ
160 170 180 190 200
ITSQVTGQIG WRREGIKYRR NELFLDVLES VNLLMSPQGQ VLSAHVSGRV
210 220 230 240 250
VMKSYLSGMP ECKFGMNDKI VIEKQGKGTA DETSKSGKQS IAIDDCTFHQ
260 270 280 290 300
CVRLSKFDSE RSISFIPPDG EFELMRYRTT KDIILPFRVI PLVREVGRTK
310 320 330 340 350
LEVKVVIKSN FKPSLLAQKI EVRIPTPLNT SGVQVICMKG KAKYKASENA
360 370 380 390 400
IVWKIKRMAG MKESQISAEI ELLPTNDKKK WARPPISMNF EVPFAPSGLK
410 420 430
VRYLKVFEPK LNYSDHDVIK WVRYIGRSGI YETRC
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ3TWV4 | Q3TWV4_MOUSE | AP-2 complex subunit mu | Ap2m1 | 433 | Annotation score: | ||
A0A338P6T4 | A0A338P6T4_MOUSE | AP-2 complex subunit mu | Ap2m1 | 119 | Annotation score: | ||
A0A338P798 | A0A338P798_MOUSE | AP-2 complex subunit mu | Ap2m1 | 82 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U27106 mRNA Translation: AAC53158.1 AF001923 , AF001913, AF001914, AF001915, AF001916, AF001917, AF001918, AF001919, AF001920, AF001921, AF001922 Genomic DNA Translation: AAC53583.1 BC056352 mRNA Translation: AAH56352.1 |
CCDSi | CCDS28048.1 |
PIRi | I49327 JC6563 |
RefSeqi | NP_033809.1, NM_009679.3 |
Genome annotation databases
Ensembli | ENSMUST00000007216; ENSMUSP00000007216; ENSMUSG00000022841 |
GeneIDi | 11773 |
KEGGi | mmu:11773 |
UCSCi | uc007ypy.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U27106 mRNA Translation: AAC53158.1 AF001923 , AF001913, AF001914, AF001915, AF001916, AF001917, AF001918, AF001919, AF001920, AF001921, AF001922 Genomic DNA Translation: AAC53583.1 BC056352 mRNA Translation: AAH56352.1 |
CCDSi | CCDS28048.1 |
PIRi | I49327 JC6563 |
RefSeqi | NP_033809.1, NM_009679.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6OWO | electron microscopy | 3.20 | M | 1-435 | [»] | |
6OWT | electron microscopy | 3.80 | M | 1-141 | [»] | |
6OXL | electron microscopy | 3.50 | M | 1-435 | [»] | |
SMRi | P84091 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 198131, 33 interactors |
ComplexPortali | CPX-5152, AP-2 Adaptor complex, alpha1 variant CPX-5153, AP-2 Adaptor complex, alpha2 variant |
IntActi | P84091, 10 interactors |
MINTi | P84091 |
STRINGi | 10090.ENSMUSP00000007216 |
PTM databases
iPTMneti | P84091 |
PhosphoSitePlusi | P84091 |
SwissPalmi | P84091 |
Proteomic databases
EPDi | P84091 |
jPOSTi | P84091 |
PaxDbi | P84091 |
PeptideAtlasi | P84091 |
PRIDEi | P84091 |
Protocols and materials databases
Antibodypediai | 33793, 526 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000007216; ENSMUSP00000007216; ENSMUSG00000022841 |
GeneIDi | 11773 |
KEGGi | mmu:11773 |
UCSCi | uc007ypy.2, mouse |
Organism-specific databases
CTDi | 1173 |
MGIi | MGI:1298405, Ap2m1 |
Phylogenomic databases
eggNOGi | KOG0938, Eukaryota |
GeneTreei | ENSGT00940000159223 |
InParanoidi | P84091 |
OMAi | ALVFEFC |
OrthoDBi | 725236at2759 |
PhylomeDBi | P84091 |
TreeFami | TF300722 |
Enzyme and pathway databases
Reactomei | R-MMU-177504, Retrograde neurotrophin signalling R-MMU-190873, Gap junction degradation R-MMU-196025, Formation of annular gap junctions R-MMU-2132295, MHC class II antigen presentation R-MMU-416993, Trafficking of GluR2-containing AMPA receptors R-MMU-437239, Recycling pathway of L1 R-MMU-5099900, WNT5A-dependent internalization of FZD4 R-MMU-5140745, WNT5A-dependent internalization of FZD2, FZD5 and ROR2 R-MMU-8856825, Cargo recognition for clathrin-mediated endocytosis R-MMU-8856828, Clathrin-mediated endocytosis R-MMU-8866427, VLDLR internalisation and degradation R-MMU-8964038, LDL clearance |
Miscellaneous databases
BioGRID-ORCSi | 11773, 10 hits in 21 CRISPR screens |
ChiTaRSi | Ap2m1, mouse |
PROi | PR:P84091 |
RNActi | P84091, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000022841, Expressed in retinal neural layer and 115 other tissues |
ExpressionAtlasi | P84091, baseline and differential |
Genevisiblei | P84091, MM |
Family and domain databases
CDDi | cd09251, AP-2_Mu2_Cterm, 1 hit cd14836, AP2_Mu_N, 1 hit |
InterProi | View protein in InterPro IPR036168, AP2_Mu_C_sf IPR043532, AP2_Mu_N IPR022775, AP_mu_sigma_su IPR001392, Clathrin_mu IPR018240, Clathrin_mu_CS IPR011012, Longin-like_dom_sf IPR028565, MHD IPR043512, Mu2_C |
Pfami | View protein in Pfam PF00928, Adap_comp_sub, 1 hit PF01217, Clat_adaptor_s, 1 hit |
PIRSFi | PIRSF005992, Clathrin_mu, 1 hit |
PRINTSi | PR00314, CLATHRINADPT |
SUPFAMi | SSF49447, SSF49447, 1 hit SSF64356, SSF64356, 1 hit |
PROSITEi | View protein in PROSITE PS00990, CLAT_ADAPTOR_M_1, 1 hit PS00991, CLAT_ADAPTOR_M_2, 1 hit PS51072, MHD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AP2M1_MOUSE | |
Accessioni | P84091Primary (citable) accession number: P84091 Secondary accession number(s): P20172, P53679 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2004 |
Last sequence update: | August 16, 2004 | |
Last modified: | December 2, 2020 | |
This is version 146 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families