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Protein

AP-2 complex subunit mu

Gene

Ap2m1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at 'Tyr-156' in membrane-associated AP-2. The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (By similarity). Plays a role in endocytosis of frizzled family members upon Wnt signaling (By similarity).By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei341Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei343Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei345Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei354Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei356Phosphatidylinositol lipid headgroupBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processEndocytosis, Protein transport, Transport
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-MMU-177504 Retrograde neurotrophin signalling
R-MMU-190873 Gap junction degradation
R-MMU-196025 Formation of annular gap junctions
R-MMU-2132295 MHC class II antigen presentation
R-MMU-416993 Trafficking of GluR2-containing AMPA receptors
R-MMU-437239 Recycling pathway of L1
R-MMU-5099900 WNT5A-dependent internalization of FZD4
R-MMU-5140745 WNT5A-dependent internalization of FZD2, FZD5 and ROR2
R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis
R-MMU-8866427 VLDLR internalisation and degradation
R-MMU-8964038 LDL clearance

Names & Taxonomyi

Protein namesi
Recommended name:
AP-2 complex subunit mu
Alternative name(s):
AP-2 mu chain
Adaptor protein complex AP-2 subunit mu
Adaptor-related protein complex 2 subunit mu
Clathrin assembly protein complex 2 mu medium chain
Clathrin coat assembly protein AP50
Clathrin coat-associated protein AP50
Mu2-adaptin
Plasma membrane adaptor AP-2 50 kDa protein
Gene namesi
Name:Ap2m1
Synonyms:Clapm1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1298405 Ap2m1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Coated pit, Membrane

Pathology & Biotechi

Disruption phenotypei

Embryonic lethal before day 3.5 postcoitus (E3.5).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001937751 – 435AP-2 complex subunit muAdd BLAST435

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei45PhosphoserineBy similarity1
Modified residuei156PhosphothreonineCombined sources1

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP84091
PaxDbiP84091
PeptideAtlasiP84091
PRIDEiP84091

PTM databases

iPTMnetiP84091
PhosphoSitePlusiP84091
SwissPalmiP84091

Expressioni

Tissue specificityi

Detected in spleen.

Gene expression databases

BgeeiENSMUSG00000022841 Expressed in 88 organ(s), highest expression level in Ammon's horn
CleanExiMM_AP2M1
ExpressionAtlasiP84091 baseline and differential
GenevisibleiP84091 MM

Interactioni

Subunit structurei

Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1) and a small adaptin (sigma-type subunit AP2S1) (By similarity). Interacts with ATP6V1H and MEGF10 (By similarity). Interacts with EGFR and TTGN1 (By similarity). Interacts with F2R (By similarity). Interacts with PIP5K1C isoform 1; tyrosine phosphorylation of PIP5K1C weakens the interaction. Does not interact with PIP5K1C isoform 3 (PubMed:16707488). Interacts with KIAA0319; required for clathrin-mediated endocytosis of KIAA0319 (By similarity). Interacts with DVL2 (via DEP domain) (By similarity). Interacts with KCNQ1; mediates estrogen-induced internalization via clathrin-coated vesicles (By similarity). Interacts with P2RX4 (via internalization motif) (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198131, 8 interactors
IntActiP84091, 10 interactors
MINTiP84091
STRINGi10090.ENSMUSP00000007216

Structurei

3D structure databases

ProteinModelPortaliP84091
SMRiP84091
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini170 – 434MHDPROSITE-ProRule annotationAdd BLAST265

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0937 Eukaryota
ENOG410XPFS LUCA
GeneTreeiENSGT00530000062779
HOGENOMiHOG000173246
HOVERGENiHBG050516
InParanoidiP84091
KOiK11826
OMAiSIKWVRY
OrthoDBiEOG091G06XI
PhylomeDBiP84091
TreeFamiTF300722

Family and domain databases

InterProiView protein in InterPro
IPR036168 AP2_Mu_C_sf
IPR022775 AP_mu_sigma_su
IPR001392 Clathrin_mu
IPR018240 Clathrin_mu_CS
IPR011012 Longin-like_dom_sf
IPR028565 MHD
PfamiView protein in Pfam
PF00928 Adap_comp_sub, 1 hit
PF01217 Clat_adaptor_s, 1 hit
PIRSFiPIRSF005992 Clathrin_mu, 1 hit
PRINTSiPR00314 CLATHRINADPT
SUPFAMiSSF49447 SSF49447, 1 hit
SSF64356 SSF64356, 1 hit
PROSITEiView protein in PROSITE
PS00990 CLAT_ADAPTOR_M_1, 1 hit
PS00991 CLAT_ADAPTOR_M_2, 1 hit
PS51072 MHD, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

P84091-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIGGLFIYNH KGEVLISRVY RDDIGRNAVD AFRVNVIHAR QQVRSPVTNI
60 70 80 90 100
ARTSFFHVKR SNIWLAAVTK QNVNAAMVFE FLYKMCDVMA AYFGKISEEN
110 120 130 140 150
IKNNFVLIYE LLDEILDFGY PQNSETGALK TFITQQGIKS QHQTKEEQSQ
160 170 180 190 200
ITSQVTGQIG WRREGIKYRR NELFLDVLES VNLLMSPQGQ VLSAHVSGRV
210 220 230 240 250
VMKSYLSGMP ECKFGMNDKI VIEKQGKGTA DETSKSGKQS IAIDDCTFHQ
260 270 280 290 300
CVRLSKFDSE RSISFIPPDG EFELMRYRTT KDIILPFRVI PLVREVGRTK
310 320 330 340 350
LEVKVVIKSN FKPSLLAQKI EVRIPTPLNT SGVQVICMKG KAKYKASENA
360 370 380 390 400
IVWKIKRMAG MKESQISAEI ELLPTNDKKK WARPPISMNF EVPFAPSGLK
410 420 430
VRYLKVFEPK LNYSDHDVIK WVRYIGRSGI YETRC
Length:435
Mass (Da):49,655
Last modified:August 16, 2004 - v1
Checksum:i82803219BA279954
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3TWV4Q3TWV4_MOUSE
AP-2 complex subunit mu
Ap2m1 mCG_128452
433Annotation score:
A0A338P6T4A0A338P6T4_MOUSE
AP-2 complex subunit mu
Ap2m1
119Annotation score:
A0A338P798A0A338P798_MOUSE
AP-2 complex subunit mu
Ap2m1
82Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27106 mRNA Translation: AAC53158.1
AF001923
, AF001913, AF001914, AF001915, AF001916, AF001917, AF001918, AF001919, AF001920, AF001921, AF001922 Genomic DNA Translation: AAC53583.1
BC056352 mRNA Translation: AAH56352.1
CCDSiCCDS28048.1
PIRiI49327
JC6563
RefSeqiNP_033809.1, NM_009679.3
UniGeneiMm.18946

Genome annotation databases

EnsembliENSMUST00000007216; ENSMUSP00000007216; ENSMUSG00000022841
GeneIDi11773
KEGGimmu:11773
UCSCiuc007ypy.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27106 mRNA Translation: AAC53158.1
AF001923
, AF001913, AF001914, AF001915, AF001916, AF001917, AF001918, AF001919, AF001920, AF001921, AF001922 Genomic DNA Translation: AAC53583.1
BC056352 mRNA Translation: AAH56352.1
CCDSiCCDS28048.1
PIRiI49327
JC6563
RefSeqiNP_033809.1, NM_009679.3
UniGeneiMm.18946

3D structure databases

ProteinModelPortaliP84091
SMRiP84091
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198131, 8 interactors
IntActiP84091, 10 interactors
MINTiP84091
STRINGi10090.ENSMUSP00000007216

PTM databases

iPTMnetiP84091
PhosphoSitePlusiP84091
SwissPalmiP84091

Proteomic databases

EPDiP84091
PaxDbiP84091
PeptideAtlasiP84091
PRIDEiP84091

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000007216; ENSMUSP00000007216; ENSMUSG00000022841
GeneIDi11773
KEGGimmu:11773
UCSCiuc007ypy.2 mouse

Organism-specific databases

CTDi1173
MGIiMGI:1298405 Ap2m1

Phylogenomic databases

eggNOGiKOG0937 Eukaryota
ENOG410XPFS LUCA
GeneTreeiENSGT00530000062779
HOGENOMiHOG000173246
HOVERGENiHBG050516
InParanoidiP84091
KOiK11826
OMAiSIKWVRY
OrthoDBiEOG091G06XI
PhylomeDBiP84091
TreeFamiTF300722

Enzyme and pathway databases

ReactomeiR-MMU-177504 Retrograde neurotrophin signalling
R-MMU-190873 Gap junction degradation
R-MMU-196025 Formation of annular gap junctions
R-MMU-2132295 MHC class II antigen presentation
R-MMU-416993 Trafficking of GluR2-containing AMPA receptors
R-MMU-437239 Recycling pathway of L1
R-MMU-5099900 WNT5A-dependent internalization of FZD4
R-MMU-5140745 WNT5A-dependent internalization of FZD2, FZD5 and ROR2
R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis
R-MMU-8866427 VLDLR internalisation and degradation
R-MMU-8964038 LDL clearance

Miscellaneous databases

ChiTaRSiAp2m1 mouse
PROiPR:P84091
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022841 Expressed in 88 organ(s), highest expression level in Ammon's horn
CleanExiMM_AP2M1
ExpressionAtlasiP84091 baseline and differential
GenevisibleiP84091 MM

Family and domain databases

InterProiView protein in InterPro
IPR036168 AP2_Mu_C_sf
IPR022775 AP_mu_sigma_su
IPR001392 Clathrin_mu
IPR018240 Clathrin_mu_CS
IPR011012 Longin-like_dom_sf
IPR028565 MHD
PfamiView protein in Pfam
PF00928 Adap_comp_sub, 1 hit
PF01217 Clat_adaptor_s, 1 hit
PIRSFiPIRSF005992 Clathrin_mu, 1 hit
PRINTSiPR00314 CLATHRINADPT
SUPFAMiSSF49447 SSF49447, 1 hit
SSF64356 SSF64356, 1 hit
PROSITEiView protein in PROSITE
PS00990 CLAT_ADAPTOR_M_1, 1 hit
PS00991 CLAT_ADAPTOR_M_2, 1 hit
PS51072 MHD, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAP2M1_MOUSE
AccessioniPrimary (citable) accession number: P84091
Secondary accession number(s): P20172, P53679
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: November 7, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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