UniProtKB - P84080 (ARF1_BOVIN)
ADP-ribosylation factor 1
ARF1
Functioni
GTP-binding protein involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD).
By similaritySites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 160 | GTP; via amide nitrogenCombined sourcesCurated | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 24 – 32 | GTPCombined sourcesCurated | 9 | |
Nucleotide bindingi | 126 – 129 | GTPCombined sourcesCurated | 4 |
GO - Molecular functioni
- GTPase activity Source: InterPro
- GTP binding Source: GO_Central
GO - Biological processi
- dendritic spine organization Source: UniProtKB
- intracellular protein transport Source: GO_Central
- long-term synaptic depression Source: UniProtKB
- regulation of Arp2/3 complex-mediated actin nucleation Source: UniProtKB
- regulation of receptor internalization Source: UniProtKB
- vesicle-mediated transport Source: GO_Central
Keywordsi
Biological process | ER-Golgi transport, Protein transport, Transport |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-BTA-199992, trans-Golgi Network Vesicle Budding R-BTA-6807878, COPI-mediated anterograde transport R-BTA-6811434, COPI-dependent Golgi-to-ER retrograde traffic R-BTA-6811438, Intra-Golgi traffic |
Names & Taxonomyi
Protein namesi | Recommended name: ADP-ribosylation factor 1 |
Gene namesi | Name:ARF1 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | HostDB:ENSBTAG00000007725 |
VGNCi | VGNC:50209, ARF1 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus
- trans-Golgi network membrane By similarity; Lipid-anchor By similarity
Other locations
- perinuclear region By similarity
- synaptosome By similarity
- postsynaptic density By similarity
- Membrane By similarity; Lipid-anchor By similarity
Cytosol
- cytosol Source: AgBase
Golgi apparatus
- Golgi apparatus Source: AgBase
Plasma Membrane
- plasma membrane Source: AgBase
Other locations
- neuron projection Source: UniProtKB-KW
- perinuclear region of cytoplasm Source: UniProtKB-SubCell
- postsynaptic density Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell junction, Cytoplasm, Golgi apparatus, Membrane, Synapse, SynaptosomePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 71 | Q → L: Mainly GTP-bound form; abolishes interaction with TMED10. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000207377 | 2 – 181 | ADP-ribosylation factor 1Add BLAST | 180 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylglycine; alternateBy similarity | 1 | |
Lipidationi | 2 | N-myristoyl glycine; alternateBy similarity | 1 |
Keywords - PTMi
Acetylation, Lipoprotein, MyristateProteomic databases
PaxDbi | P84080 |
PeptideAtlasi | P84080 |
PRIDEi | P84080 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000007725, Expressed in thyroid gland and 96 other tissues |
Interactioni
Subunit structurei
Interacts (when activated) with GGA1, GGA2 and GGA3; the interaction is required for proper subcellular location of GGA1, GGA2 and GGA3 (By similarity).
Interacts with ARHGAP21, ASAP2, GGA1, HERC1, PRKCABP, PIP5K1B, TMED2, PSCD2, TMED10 and GRIA2 (PubMed:11703931, PubMed:14654833).
Interacts with ARFGAP1, which hydrolyzes GTP and thus, regulates its function.
Interacts with PI4KB in the Golgi complex.
Interacts with NCS1/FREQ in the Golgi and at the plasma membrane.
Interacts with PLEKHA3.
Interacts with PLEKHA8; the interaction, together with phosphatidylinositol 4-phosphate binding, is required for FAPP2-mediated glucosylceramide transfer activity.
Interacts (activated) with PICK1 (via PDZ domain); the interaction blocks Arp2/3 complex inhibition (By similarity).
Interacts with IQSEC1 (PubMed:24058294).
Interacts with C9orf72 (By similarity).
By similarity3 PublicationsBinary interactionsi
P84080
With | #Exp. | IntAct |
---|---|---|
Ncs1 [P62168] from Rattus norvegicus. | 2 | EBI-449051,EBI-907774 |
Protein-protein interaction databases
BioGRIDi | 160195, 1 interactor |
IntActi | P84080, 8 interactors |
MINTi | P84080 |
STRINGi | 9913.ENSBTAP00000010159 |
Structurei
Secondary structure
3D structure databases
BMRBi | P84080 |
SMRi | P84080 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P84080 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0070, Eukaryota |
GeneTreei | ENSGT00950000183080 |
HOGENOMi | CLU_040729_9_3_1 |
InParanoidi | P84080 |
OMAi | NDRERIP |
OrthoDBi | 1362554at2759 |
TreeFami | TF300808 |
Family and domain databases
CDDi | cd04150, Arf1_5_like, 1 hit |
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR045872, Arf1_5_like IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR024156, Small_GTPase_ARF IPR006689, Small_GTPase_ARF/SAR |
PANTHERi | PTHR11711, PTHR11711, 1 hit |
Pfami | View protein in Pfam PF00025, Arf, 1 hit |
PRINTSi | PR00328, SAR1GTPBP |
SMARTi | View protein in SMART SM00178, SAR, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51417, ARF, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MGNIFANLFK GLFGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG
60 70 80 90 100
FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV
110 120 130 140 150
NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH
160 170 180
RNWYIQATCA TSGDGLYEGL DWLSNQLRNQ K
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J03275 mRNA Translation: AAA30361.1 BC102874 mRNA Translation: AAI02875.1 BC140532 mRNA Translation: AAI40533.1 |
PIRi | A36167 |
RefSeqi | NP_788826.1, NM_176653.4 XP_005208456.1, XM_005208399.3 XP_005208457.1, XM_005208400.3 |
Genome annotation databases
Ensembli | ENSBTAT00000010159; ENSBTAP00000010159; ENSBTAG00000007725 |
GeneIDi | 338058 |
KEGGi | bta:338058 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J03275 mRNA Translation: AAA30361.1 BC102874 mRNA Translation: AAI02875.1 BC140532 mRNA Translation: AAI40533.1 |
PIRi | A36167 |
RefSeqi | NP_788826.1, NM_176653.4 XP_005208456.1, XM_005208399.3 XP_005208457.1, XM_005208400.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1R8Q | X-ray | 1.86 | A/B | 1-181 | [»] | |
1R8S | X-ray | 1.46 | A | 18-181 | [»] | |
1S9D | X-ray | 1.80 | A | 18-181 | [»] | |
4C0A | X-ray | 3.30 | C/D/G/H | 18-181 | [»] | |
BMRBi | P84080 | |||||
SMRi | P84080 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 160195, 1 interactor |
IntActi | P84080, 8 interactors |
MINTi | P84080 |
STRINGi | 9913.ENSBTAP00000010159 |
Proteomic databases
PaxDbi | P84080 |
PeptideAtlasi | P84080 |
PRIDEi | P84080 |
Genome annotation databases
Ensembli | ENSBTAT00000010159; ENSBTAP00000010159; ENSBTAG00000007725 |
GeneIDi | 338058 |
KEGGi | bta:338058 |
Organism-specific databases
CTDi | 375 |
VEuPathDBi | HostDB:ENSBTAG00000007725 |
VGNCi | VGNC:50209, ARF1 |
Phylogenomic databases
eggNOGi | KOG0070, Eukaryota |
GeneTreei | ENSGT00950000183080 |
HOGENOMi | CLU_040729_9_3_1 |
InParanoidi | P84080 |
OMAi | NDRERIP |
OrthoDBi | 1362554at2759 |
TreeFami | TF300808 |
Enzyme and pathway databases
Reactomei | R-BTA-199992, trans-Golgi Network Vesicle Budding R-BTA-6807878, COPI-mediated anterograde transport R-BTA-6811434, COPI-dependent Golgi-to-ER retrograde traffic R-BTA-6811438, Intra-Golgi traffic |
Miscellaneous databases
EvolutionaryTracei | P84080 |
Gene expression databases
Bgeei | ENSBTAG00000007725, Expressed in thyroid gland and 96 other tissues |
Family and domain databases
CDDi | cd04150, Arf1_5_like, 1 hit |
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR045872, Arf1_5_like IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR024156, Small_GTPase_ARF IPR006689, Small_GTPase_ARF/SAR |
PANTHERi | PTHR11711, PTHR11711, 1 hit |
Pfami | View protein in Pfam PF00025, Arf, 1 hit |
PRINTSi | PR00328, SAR1GTPBP |
SMARTi | View protein in SMART SM00178, SAR, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51417, ARF, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ARF1_BOVIN | |
Accessioni | P84080Primary (citable) accession number: P84080 Secondary accession number(s): A5D7F1 Q3SZG1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2004 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 153 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families