ARF1

Functionⁱ

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD).

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Binding siteⁱ	160	GTP; via amide nitrogenCombined sourcesCurated		1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	24 – 32	GTPCombined sourcesCurated		9
Nucleotide bindingⁱ	126 – 129	GTPCombined sourcesCurated		4

GO - Molecular functionⁱ

GTP binding Source: UniProtKB-KW
protein domain specific binding Source: Ensembl
protein heterodimerization activity Source: Ensembl

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cellular copper ion homeostasis Source: Ensembl
cellular response to virus Source: Ensembl
dendritic spine organization Source: UniProtKB
long term synaptic depression Source: UniProtKB
protein transport Source: UniProtKB-KW
regulation of Arp2/3 complex-mediated actin nucleation Source: UniProtKB
regulation of receptor internalization Source: UniProtKB
vesicle-mediated transport Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	ER-Golgi transport, Protein transport, Transport
Ligand	GTP-binding, Nucleotide-binding

Enzyme and pathway databases

Reactomeⁱ	R-BTA-1660499 Synthesis of PIPs at the plasma membrane R-BTA-1660514 Synthesis of PIPs at the Golgi membrane R-BTA-2132295 MHC class II antigen presentation R-BTA-421837 Clathrin derived vesicle budding R-BTA-432720 Lysosome Vesicle Biogenesis R-BTA-432722 Golgi Associated Vesicle Biogenesis R-BTA-6807878 COPI-mediated anterograde transport R-BTA-6811434 COPI-dependent Golgi-to-ER retrograde traffic R-BTA-6811438 Intra-Golgi traffic

Reactomeⁱ

R-BTA-1660499 Synthesis of PIPs at the plasma membrane
R-BTA-1660514 Synthesis of PIPs at the Golgi membrane
R-BTA-2132295 MHC class II antigen presentation
R-BTA-421837 Clathrin derived vesicle budding
R-BTA-432720 Lysosome Vesicle Biogenesis
R-BTA-432722 Golgi Associated Vesicle Biogenesis
R-BTA-6807878 COPI-mediated anterograde transport
R-BTA-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-BTA-6811438 Intra-Golgi traffic

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: ADP-ribosylation factor 1
Gene namesⁱ	Name:ARF1
Organismⁱ	Bos taurus (Bovine)
Taxonomic identifierⁱ	9913 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos
Proteomesⁱ	UP000009136 Componentⁱ: Chromosome 7

Organism-specific databases

VGNCⁱ	VGNC:50209 ARF1

VGNCⁱ

VGNC:50209 ARF1

Keywords - Cellular componentⁱ

Cell junction, Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Postsynaptic cell membrane, Synapse, Synaptosome

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	71	Q → L: Mainly GTP-bound form; abolishes interaction with TMED10. 1 Publication		1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedBy similarity
ChainⁱPRO_0000207377	2 – 181	ADP-ribosylation factor 1Add BLAST		180

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	2	N-acetylglycine; alternateBy similarity		1
Lipidationⁱ	2	N-myristoyl glycine; alternateBy similarity		1

Keywords - PTMⁱ

Acetylation, Lipoprotein, Myristate

Proteomic databases

PaxDbⁱ	P84080
PeptideAtlas More...PeptideAtlasⁱ	P84080
PRIDEⁱ	P84080

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSBTAG00000007725

Bgeeⁱ

ENSBTAG00000007725

Interactionⁱ

Subunit structureⁱ

Interacts with ARHGAP21, ASAP2, GGA1, HERC1, PRKCABP, PIP5K1B, TMED2, PSCD2, TMED10 and GRIA2. Interacts with ARFGAP1, which hydrolyzes GTP and thus, regulates its function. Interacts with PI4KB in the Golgi complex. Interacts with NCS1/FREQ in the Golgi and at the plasma membrane. Interacts with PLEKHA3. Interacts with PLEKHA8; the interaction, together with phosphatidylinositol 4-phosphate binding, is required for FAPP2-mediated glucosylceramide transfer activity. Interacts (activated) with PICK1 (via PDZ domain); the interaction blocks Arp2/3 complex inhibition (By similarity). Interacts with IQSEC1 (PubMed:24058294). Interacts with C9orf72 (By similarity).By similarity3 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Asap1	Q9QWY8	2	EBI-449051,EBI-698498	From Mus musculus.
GGA1	Q9UJY5	2	EBI-449051,EBI-447141	From Homo sapiens.
GGA2	Q9UJY4	2	EBI-449051,EBI-447646	From Homo sapiens.
GGA3	Q9NZ52	3	EBI-449051,EBI-447404	From Homo sapiens.
Ncs1	P62168	2	EBI-449051,EBI-907774	From Rattus norvegicus.

Show more details

GO - Molecular functionⁱ

protein domain specific binding Source: Ensembl
protein heterodimerization activity Source: Ensembl

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	160195, 1 interactor
IntActⁱ	P84080, 9 interactors
Molecular INTeraction database More...MINTⁱ	P84080
STRINGⁱ	9913.ENSBTAP00000010159

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Show more details

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	4 – 7	Combined sources	4
Beta strandⁱ	19 – 23	Combined sources	5
Helixⁱ	30 – 40	Combined sources	11
Beta strandⁱ	49 – 51	Combined sources	3
Beta strandⁱ	53 – 57	Combined sources	5
Beta strandⁱ	62 – 67	Combined sources	6
Helixⁱ	72 – 74	Combined sources	3
Helixⁱ	75 – 81	Combined sources	7
Turnⁱ	82 – 84	Combined sources	3
Beta strandⁱ	86 – 93	Combined sources	8
Helixⁱ	97 – 99	Combined sources	3
Helixⁱ	100 – 111	Combined sources	12
Helixⁱ	114 – 116	Combined sources	3
Beta strandⁱ	120 – 126	Combined sources	7
Helixⁱ	136 – 142	Combined sources	7
Helixⁱ	145 – 147	Combined sources	3
Beta strandⁱ	153 – 157	Combined sources	5
Turnⁱ	160 – 163	Combined sources	4
Helixⁱ	166 – 176	Combined sources	11

3D structure databases

ProteinModelPortalⁱ	P84080
SMRⁱ	P84080
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P84080

EvolutionaryTraceⁱ

P84080

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGⁱ	KOG0070 Eukaryota COG1100 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00780000121855
HOGENOMⁱ	HOG000163691
HOVERGENⁱ	HBG002073
InParanoidⁱ	P84080
KEGG Orthology (KO) More...KOⁱ	K07937
OMAⁱ	HYYSNTD
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0S7S
TreeFamⁱ	TF300808

Family and domain databases

InterProⁱ	View protein in InterPro IPR027417 P-loop_NTPase IPR005225 Small_GTP-bd_dom IPR024156 Small_GTPase_ARF IPR006689 Small_GTPase_ARF/SAR
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00025 Arf, 1 hit
PRINTSⁱ	PR00328 SAR1GTPBP
SUPFAMⁱ	SSF52540 SSF52540, 1 hit
TIGRFAMsⁱ	TIGR00231 small_GTP, 1 hit
PROSITEⁱ	View protein in PROSITE PS51417 ARF, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P84080-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MGNIFANLFK GLFGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG 
        60         70         80         90        100
FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV 
       110        120        130        140        150
NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH 
       160        170        180 
RNWYIQATCA TSGDGLYEGL DWLSNQLRNQ K

Length:181

Mass (Da):20,697

Last modified:January 23, 2007 - v2

Checksum:ⁱAAC773D4A60186B6

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	J03275 mRNA Translation: AAA30361.1 BC102874 mRNA Translation: AAI02875.1 BC140532 mRNA Translation: AAI40533.1
PIRⁱ	A36167
NCBI Reference Sequences More...RefSeqⁱ	NP_788826.1, NM_176653.4 XP_005208456.1, XM_005208399.3 XP_005208457.1, XM_005208400.3
UniGeneⁱ	Bt.91076 Bt.927

Genome annotation databases

Ensemblⁱ	ENSBTAT00000010159; ENSBTAP00000010159; ENSBTAG00000007725
GeneIDⁱ	338058
KEGGⁱ	bta:338058

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length
P84080	ADP-ribosylation factor 1	)	HUMAN		181
ADP-ribosylation factor 1		MACFA		181
ADP-ribosylation factor 1	)	MOUSE		181
ADP-ribosylation factor 1	)	RAT		181
ADP-ribosylation factor 1		CALJA		181
+63

Protein	Similar proteins		Species	Score	Length
P84080	ADP-ribosylation factor 1	)	HUMAN		181
ADP-ribosylation factor 1		MACFA		181
ADP-ribosylation factor 1	)	MOUSE		181
ADP-ribosylation factor 1	)	RAT		181
ADP-ribosylation factor 1		CALJA		181
+71

Protein	Similar proteins		Species	Score	Length
P84080	ADP-ribosylation factor 1	)	HUMAN		181
ADP-ribosylation factor 1		MACFA		181
ADP-ribosylation factor 1	)	MOUSE		181
ADP-ribosylation factor 1	)	RAT		181
ADP-ribosylation factor 1		CALJA		181
+590

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	J03275 mRNA Translation: AAA30361.1 BC102874 mRNA Translation: AAI02875.1 BC140532 mRNA Translation: AAI40533.1
PIRⁱ	A36167
NCBI Reference Sequences More...RefSeqⁱ	NP_788826.1, NM_176653.4 XP_005208456.1, XM_005208399.3 XP_005208457.1, XM_005208400.3
UniGeneⁱ	Bt.91076 Bt.927

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1R8Q	X-ray	1.86	A/B	1-181	[»]
	1R8S	X-ray	1.46	A	18-181	[»]
	1S9D	X-ray	1.80	A	18-181	[»]
	4C0A	X-ray	3.30	C/D/G/H	18-181	[»]
ProteinModelPortalⁱ	P84080
SMRⁱ	P84080
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	160195, 1 interactor
IntActⁱ	P84080, 9 interactors
Molecular INTeraction database More...MINTⁱ	P84080
STRINGⁱ	9913.ENSBTAP00000010159

Proteomic databases

PaxDbⁱ	P84080
PeptideAtlas More...PeptideAtlasⁱ	P84080
PRIDEⁱ	P84080

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSBTAT00000010159; ENSBTAP00000010159; ENSBTAG00000007725
GeneIDⁱ	338058
KEGGⁱ	bta:338058

Organism-specific databases

CTDⁱ	375
VGNCⁱ	VGNC:50209 ARF1

Phylogenomic databases

eggNOGⁱ	KOG0070 Eukaryota COG1100 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00780000121855
HOGENOMⁱ	HOG000163691
HOVERGENⁱ	HBG002073
InParanoidⁱ	P84080
KEGG Orthology (KO) More...KOⁱ	K07937
OMAⁱ	HYYSNTD
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0S7S
TreeFamⁱ	TF300808

Enzyme and pathway databases

Reactomeⁱ	R-BTA-1660499 Synthesis of PIPs at the plasma membrane R-BTA-1660514 Synthesis of PIPs at the Golgi membrane R-BTA-2132295 MHC class II antigen presentation R-BTA-421837 Clathrin derived vesicle budding R-BTA-432720 Lysosome Vesicle Biogenesis R-BTA-432722 Golgi Associated Vesicle Biogenesis R-BTA-6807878 COPI-mediated anterograde transport R-BTA-6811434 COPI-dependent Golgi-to-ER retrograde traffic R-BTA-6811438 Intra-Golgi traffic

Reactomeⁱ

R-BTA-1660499 Synthesis of PIPs at the plasma membrane
R-BTA-1660514 Synthesis of PIPs at the Golgi membrane
R-BTA-2132295 MHC class II antigen presentation
R-BTA-421837 Clathrin derived vesicle budding
R-BTA-432720 Lysosome Vesicle Biogenesis
R-BTA-432722 Golgi Associated Vesicle Biogenesis
R-BTA-6807878 COPI-mediated anterograde transport
R-BTA-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-BTA-6811438 Intra-Golgi traffic

Miscellaneous databases

EvolutionaryTraceⁱ	P84080

EvolutionaryTraceⁱ

P84080

Gene expression databases

Bgeeⁱ	ENSBTAG00000007725

Bgeeⁱ

ENSBTAG00000007725

Family and domain databases

InterProⁱ	View protein in InterPro IPR027417 P-loop_NTPase IPR005225 Small_GTP-bd_dom IPR024156 Small_GTPase_ARF IPR006689 Small_GTPase_ARF/SAR
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00025 Arf, 1 hit
PRINTSⁱ	PR00328 SAR1GTPBP
SUPFAMⁱ	SSF52540 SSF52540, 1 hit
TIGRFAMsⁱ	TIGR00231 small_GTP, 1 hit
PROSITEⁱ	View protein in PROSITE PS51417 ARF, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ARF1_BOVIN
Accessionⁱ	P84080Primary (citable) accession number: P84080 Secondary accession number(s): A5D7F1 , P10947, P32889, Q3SZG1
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
	Last sequence update:	January 23, 2007
	Last modified:	July 18, 2018
	This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

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Supporting data

UniProtKB - P84080 (ARF1_BOVIN)

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ADP-ribosylation factor 1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Golgi apparatus

Other locations

Cytosol

Golgi apparatus

Plasma Membrane

Other locations

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ