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Protein

ADP-ribosylation factor 1

Gene

Arf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei160GTP; via amide nitrogenCombined sources1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 32GTPCombined sources1 Publication9
Nucleotide bindingi126 – 129GTPCombined sources1 Publication4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processER-Golgi transport, Protein transport, Transport
LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 1
Gene namesi
Name:Arf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:99431 Arf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Postsynaptic cell membrane, Synapse, Synaptosome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075273

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002073802 – 181ADP-ribosylation factor 1Add BLAST180

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylglycine; alternateBy similarity1
Lipidationi2N-myristoyl glycine; alternateBy similarity1

Keywords - PTMi

Acetylation, Lipoprotein, Myristate

Proteomic databases

EPDiP84078
MaxQBiP84078
PaxDbiP84078
PeptideAtlasiP84078
PRIDEiP84078
TopDownProteomicsiP84078

PTM databases

iPTMnetiP84078
PhosphoSitePlusiP84078
SwissPalmiP84078

Expressioni

Gene expression databases

BgeeiENSMUSG00000048076 Expressed in 47 organ(s), highest expression level in colon
CleanExiMM_ARF1
GenevisibleiP84078 MM

Interactioni

Subunit structurei

Interacts with ARHGAP21, ASAP2, GGA1, HERC1, PRKCABP, PIP5K1B, TMED2, PSCD2, TMED10 and GRIA2. Interacts with ARFGAP1, which hydrolyzes GTP and thus, regulates its function. Interacts with PI4KB in the Golgi complex. Interacts with NCS1/FREQ in the Golgi and at the plasma membrane. Interacts with PLEKHA3. Interacts with PLEKHA8; the interaction, together with phosphatidylinositol 4-phosphate binding, is required for FAPP2-mediated glucosylceramide transfer activity. Interacts (activated) with PICK1 (via PDZ domain); the interaction blocks Arp2/3 complex inhibition. Interacts with IQSEC1 (By similarity). Interacts with C9orf72 (PubMed:27723745).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ARHGAP21Q5T5U33EBI-2308190,EBI-1642518From Homo sapiens.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198184, 5 interactors
CORUMiP84078
IntActiP84078, 8 interactors
MINTiP84078
STRINGi10090.ENSMUSP00000079905

Structurei

Secondary structure

1181
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP84078
SMRiP84078
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP84078

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0070 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00780000121855
HOVERGENiHBG002073
InParanoidiP84078
KOiK07937
OMAiHYYSNTD
OrthoDBiEOG091G0S7S
PhylomeDBiP84078
TreeFamiTF300808

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR006689 Small_GTPase_ARF/SAR
PfamiView protein in Pfam
PF00025 Arf, 1 hit
PRINTSiPR00328 SAR1GTPBP
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51417 ARF, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P84078-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGNIFANLFK GLFGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG
60 70 80 90 100
FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV
110 120 130 140 150
NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH
160 170 180
RNWYIQATCA TSGDGLYEGL DWLSNQLRNQ K
Length:181
Mass (Da):20,697
Last modified:January 23, 2007 - v2
Checksum:iAAC773D4A60186B6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti168E → K in BAE31170 (PubMed:16141072).Curated1
Sequence conflicti174S → C in BAE28685 (PubMed:16141072).Curated1
Sequence conflicti180Q → R in BAE40054 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87898 mRNA Translation: BAA13490.1
AK148458 mRNA Translation: BAE28566.1
AK148873 mRNA Translation: BAE28685.1
AK150092 mRNA Translation: BAE29300.1
AK150699 mRNA Translation: BAE29778.1
AK151223 mRNA Translation: BAE30215.1
AK151225 mRNA Translation: BAE30217.1
AK151696 mRNA Translation: BAE30620.1
AK151821 mRNA Translation: BAE30718.1
AK152015 mRNA Translation: BAE30877.1
AK152382 mRNA Translation: BAE31170.1
AK152479 mRNA Translation: BAE31253.1
AK153132 mRNA Translation: BAE31745.1
AK159174 mRNA Translation: BAE34874.1
AK167000 mRNA Translation: BAE39179.1
AK167129 mRNA Translation: BAE39276.1
AK167543 mRNA Translation: BAE39610.1
AK167931 mRNA Translation: BAE39935.1
AK168081 mRNA Translation: BAE40054.1
AK168686 mRNA Translation: BAE40532.1
AK169166 mRNA Translation: BAE40945.1
AK170586 mRNA Translation: BAE41895.1
AL645854 Genomic DNA No translation available.
BC031986 mRNA Translation: AAH31986.1
CCDSiCCDS24765.1
PIRiJC4945
RefSeqiNP_001123880.1, NM_001130408.1
NP_031502.1, NM_007476.3
UniGeneiMm.371546

Genome annotation databases

EnsembliENSMUST00000061242; ENSMUSP00000079905; ENSMUSG00000048076
ENSMUST00000163300; ENSMUSP00000126120; ENSMUSG00000048076
GeneIDi11840
KEGGimmu:11840
UCSCiuc007jdm.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87898 mRNA Translation: BAA13490.1
AK148458 mRNA Translation: BAE28566.1
AK148873 mRNA Translation: BAE28685.1
AK150092 mRNA Translation: BAE29300.1
AK150699 mRNA Translation: BAE29778.1
AK151223 mRNA Translation: BAE30215.1
AK151225 mRNA Translation: BAE30217.1
AK151696 mRNA Translation: BAE30620.1
AK151821 mRNA Translation: BAE30718.1
AK152015 mRNA Translation: BAE30877.1
AK152382 mRNA Translation: BAE31170.1
AK152479 mRNA Translation: BAE31253.1
AK153132 mRNA Translation: BAE31745.1
AK159174 mRNA Translation: BAE34874.1
AK167000 mRNA Translation: BAE39179.1
AK167129 mRNA Translation: BAE39276.1
AK167543 mRNA Translation: BAE39610.1
AK167931 mRNA Translation: BAE39935.1
AK168081 mRNA Translation: BAE40054.1
AK168686 mRNA Translation: BAE40532.1
AK169166 mRNA Translation: BAE40945.1
AK170586 mRNA Translation: BAE41895.1
AL645854 Genomic DNA No translation available.
BC031986 mRNA Translation: AAH31986.1
CCDSiCCDS24765.1
PIRiJC4945
RefSeqiNP_001123880.1, NM_001130408.1
NP_031502.1, NM_007476.3
UniGeneiMm.371546

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1O3YX-ray1.50A/B18-181[»]
2J59X-ray2.10A/B/C/D/E/F18-181[»]
ProteinModelPortaliP84078
SMRiP84078
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198184, 5 interactors
CORUMiP84078
IntActiP84078, 8 interactors
MINTiP84078
STRINGi10090.ENSMUSP00000079905

Chemistry databases

ChEMBLiCHEMBL1075273

PTM databases

iPTMnetiP84078
PhosphoSitePlusiP84078
SwissPalmiP84078

Proteomic databases

EPDiP84078
MaxQBiP84078
PaxDbiP84078
PeptideAtlasiP84078
PRIDEiP84078
TopDownProteomicsiP84078

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000061242; ENSMUSP00000079905; ENSMUSG00000048076
ENSMUST00000163300; ENSMUSP00000126120; ENSMUSG00000048076
GeneIDi11840
KEGGimmu:11840
UCSCiuc007jdm.2 mouse

Organism-specific databases

CTDi375
MGIiMGI:99431 Arf1

Phylogenomic databases

eggNOGiKOG0070 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00780000121855
HOVERGENiHBG002073
InParanoidiP84078
KOiK07937
OMAiHYYSNTD
OrthoDBiEOG091G0S7S
PhylomeDBiP84078
TreeFamiTF300808

Miscellaneous databases

EvolutionaryTraceiP84078
PROiPR:P84078
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000048076 Expressed in 47 organ(s), highest expression level in colon
CleanExiMM_ARF1
GenevisibleiP84078 MM

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR006689 Small_GTPase_ARF/SAR
PfamiView protein in Pfam
PF00025 Arf, 1 hit
PRINTSiPR00328 SAR1GTPBP
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51417 ARF, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiARF1_MOUSE
AccessioniPrimary (citable) accession number: P84078
Secondary accession number(s): P10947
, P32889, Q3THZ2, Q3U849, Q3UDG1, Q3UF76
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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