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UniProtKB - P83967 (ACT6_DROME)
Protein
Actin, indirect flight muscle
Gene
Act88F
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Required for proper formation of indirect flight muscle (IFM) myofibrils.
Multiple isoforms are involved in various cellular functions such as cytoskeleton structure, cell mobility, chromosome movement and muscle contraction.
Miscellaneous
Abnormalities in Act88F-ifm37 flight muscles result from incorporation of the mutant actin isoform into assembling myofibrils.
In Drosophila there are 6 closely related actin genes.
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
GO - Biological processi
- mitotic cytokinesis Source: GO_Central
- muscle thin filament assembly Source: FlyBase
- skeletal myofibril assembly Source: FlyBase
Keywordsi
Molecular function | Muscle protein |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
SignaLinki | P83967 |
Names & Taxonomyi
Protein namesi | Recommended name: Actin, indirect flight muscleAlternative name(s): Actin-88F |
Gene namesi | Name:Act88F ORF Names:CG5178 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0000047, Act88F |
VEuPathDBi | VectorBase:FBgn0000047 |
Subcellular locationi
Cytoskeleton
Cytoskeleton
- striated muscle thin filament Source: FlyBase
Other locations
Keywords - Cellular componenti
Cytoplasm, CytoskeletonPathology & Biotechi
Disruption phenotypei
Homozygous mutants are viable and fertile, but fail to form proper IFMs and are flightless. The IFMs of these mutants display mislocalized Smn and Actn.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 357 – 376 | Missing in allele Act88F-ifm(3)7. 1 PublicationAdd BLAST | 20 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
PropeptideiPRO_0000000668 | 1 – 2 | Removed in mature formBy similarity | 2 | |
ChainiPRO_0000000669 | 3 – 376 | Actin, indirect flight muscleAdd BLAST | 374 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 3 | N-acetylaspartateBy similarity | 1 | |
Modified residuei | 45 | Methionine sulfoxide1 Publication | 1 | |
Modified residuei | 48 | Methionine sulfoxide1 Publication | 1 | |
Modified residuei | 74 | Tele-methylhistidineBy similarity | 1 |
Post-translational modificationi
Oxidation of Met-45 by Mical to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.1 Publication
Keywords - PTMi
Acetylation, Methylation, OxidationProteomic databases
PaxDbi | P83967 |
PRIDEi | P83967 |
PTM databases
SwissPalmi | P83967 |
Expressioni
Tissue specificityi
In adult thorax, expressed in the IFMs.1 Publication
Developmental stagei
Expressed throughout development. Expression is weak during embryonic stages and peaks during larval and pupal stages.1 Publication
Gene expression databases
Bgeei | FBgn0000047, Expressed in second segment of antenna (Drosophila) and 25 other tissues |
ExpressionAtlasi | P83967, baseline and differential |
Genevisiblei | P83967, DM |
Interactioni
Binary interactionsi
Protein-protein interaction databases
BioGRIDi | 66947, 27 interactors |
IntActi | P83967, 13 interactors |
STRINGi | 7227.FBpp0082597 |
Family & Domainsi
Sequence similaritiesi
Belongs to the actin family.Curated
Phylogenomic databases
eggNOGi | KOG0676, Eukaryota |
GeneTreei | ENSGT00940000166560 |
HOGENOMi | CLU_027965_0_2_1 |
InParanoidi | P83967 |
OMAi | THNTIHA |
OrthoDBi | 649708at2759 |
PhylomeDBi | P83967 |
Family and domain databases
InterProi | View protein in InterPro IPR004000, Actin IPR020902, Actin/actin-like_CS IPR004001, Actin_CS IPR043129, ATPase_NBD |
PANTHERi | PTHR11937, PTHR11937, 1 hit |
Pfami | View protein in Pfam PF00022, Actin, 1 hit |
PRINTSi | PR00190, ACTIN |
SMARTi | View protein in SMART SM00268, ACTIN, 1 hit |
SUPFAMi | SSF53067, SSF53067, 2 hits |
PROSITEi | View protein in PROSITE PS00406, ACTINS_1, 1 hit PS00432, ACTINS_2, 1 hit PS01132, ACTINS_ACT_LIKE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P83967-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MCDDDAGALV IDNGSGMCKA GFAGDDAPRA VFPSIVGRPR HQGVMVGMGQ
60 70 80 90 100
KDSYVGDEAQ SKRGILTLKY PIEHGIITNW DDMEKIWHHT FYNELRVAPE
110 120 130 140 150
EHPVLLTEAP LNPKANREKM TQIMFETFNS PAMYVAIQAV LSLYASGRTT
160 170 180 190 200
GIVLDSGDGV SHTVPIYEGF ALPHAILRLD LAGRDLTDYL MKILTERGYS
210 220 230 240 250
FTTTAEREIV RDIKEKLCYV ALDFEQEMAT AAASTSLEKS YELPDGQVIT
260 270 280 290 300
IGNERFRCPE ALFQPSFLGM ESCGIHETVY NSIMKCDVDI RKDLYANSVL
310 320 330 340 350
SGGTTMYPGI ADRMQKEITA LAPSTIKIKI IAPPERKYSV WIGGSILASL
360 370
STFQQMWISK QEYDESGPGI VHRKCF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 200 | S → T in AAA28321 (PubMed:6405041).Curated | 1 | |
Sequence conflicti | 213 | I → T in AAA28321 (PubMed:6405041).Curated | 1 | |
Sequence conflicti | 226 | Q → D in AAA28321 (PubMed:6405041).Curated | 1 | |
Sequence conflicti | 320 | A → T in AAA28321 (PubMed:6405041).Curated | 1 | |
Sequence conflicti | 345 | S → L in AAA28321 (PubMed:6405041).Curated | 1 | |
Sequence conflicti | 369 | G → S in AAA28321 (PubMed:6405041).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M18826 Genomic DNA Translation: AAA28323.1 K02065 Genomic DNA Translation: AAA28322.1 AB003910 Genomic DNA Translation: BAA20058.1 AE014297 Genomic DNA Translation: AAF55198.1 M18830 Genomic DNA Translation: AAA28321.1 |
PIRi | A03003, ATFF8 |
RefSeqi | NP_524367.1, NM_079643.2 |
Genome annotation databases
EnsemblMetazoai | FBtr0083143; FBpp0082597; FBgn0000047 |
GeneIDi | 41885 |
KEGGi | dme:Dmel_CG5178 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M18826 Genomic DNA Translation: AAA28323.1 K02065 Genomic DNA Translation: AAA28322.1 AB003910 Genomic DNA Translation: BAA20058.1 AE014297 Genomic DNA Translation: AAF55198.1 M18830 Genomic DNA Translation: AAA28321.1 |
PIRi | A03003, ATFF8 |
RefSeqi | NP_524367.1, NM_079643.2 |
3D structure databases
AlphaFoldDBi | P83967 |
SMRi | P83967 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 66947, 27 interactors |
IntActi | P83967, 13 interactors |
STRINGi | 7227.FBpp0082597 |
PTM databases
SwissPalmi | P83967 |
Proteomic databases
PaxDbi | P83967 |
PRIDEi | P83967 |
Protocols and materials databases
DNASUi | 41885 |
Genome annotation databases
EnsemblMetazoai | FBtr0083143; FBpp0082597; FBgn0000047 |
GeneIDi | 41885 |
KEGGi | dme:Dmel_CG5178 |
Organism-specific databases
CTDi | 41885 |
FlyBasei | FBgn0000047, Act88F |
VEuPathDBi | VectorBase:FBgn0000047 |
Phylogenomic databases
eggNOGi | KOG0676, Eukaryota |
GeneTreei | ENSGT00940000166560 |
HOGENOMi | CLU_027965_0_2_1 |
InParanoidi | P83967 |
OMAi | THNTIHA |
OrthoDBi | 649708at2759 |
PhylomeDBi | P83967 |
Enzyme and pathway databases
SignaLinki | P83967 |
Miscellaneous databases
BioGRID-ORCSi | 41885, 0 hits in 3 CRISPR screens |
ChiTaRSi | Act88F, fly |
GenomeRNAii | 41885 |
PROi | PR:P83967 |
Gene expression databases
Bgeei | FBgn0000047, Expressed in second segment of antenna (Drosophila) and 25 other tissues |
ExpressionAtlasi | P83967, baseline and differential |
Genevisiblei | P83967, DM |
Family and domain databases
InterProi | View protein in InterPro IPR004000, Actin IPR020902, Actin/actin-like_CS IPR004001, Actin_CS IPR043129, ATPase_NBD |
PANTHERi | PTHR11937, PTHR11937, 1 hit |
Pfami | View protein in Pfam PF00022, Actin, 1 hit |
PRINTSi | PR00190, ACTIN |
SMARTi | View protein in SMART SM00268, ACTIN, 1 hit |
SUPFAMi | SSF53067, SSF53067, 2 hits |
PROSITEi | View protein in PROSITE PS00406, ACTINS_1, 1 hit PS00432, ACTINS_2, 1 hit PS01132, ACTINS_ACT_LIKE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ACT6_DROME | |
Accessioni | P83967Primary (citable) accession number: P83967 Secondary accession number(s): P02575, P45893, Q9VF62 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | June 7, 2004 | |
Last modified: | May 25, 2022 | |
This is version 142 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families