UniProtKB - P83940 (ELOC_MOUSE)
Elongin-C
Eloc
Functioni
SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex) (By similarity).
In embryonic stem cells, the elongin BC complex is recruited by EPOP to Polycomb group (PcG) target genes in order generate genomic region that display both active and repressive chromatin properties, an important feature of pluripotent stem cells (PubMed:27863225, PubMed:27863226).
By similarity2 PublicationsCore component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. This includes the von Hippel-Lindau ubiquitination complex CBC(VHL). By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes. A number of ECS complexes (containing either KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B or FEM1C as substrate-recognition component) are part of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation.
By similarity: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.By similarityView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
GO - Molecular functioni
- protein-containing complex binding Source: MGI
- protein-macromolecule adaptor activity Source: GO_Central
- transcription corepressor binding Source: MGI
GO - Biological processi
- positive regulation of transcription elongation from RNA polymerase II promoter Source: MGI
- protein ubiquitination Source: UniProtKB
- transcription initiation from RNA polymerase II promoter Source: MGI
- ubiquitin-dependent protein catabolic process Source: GO_Central
Keywordsi
Biological process | Transcription, Transcription regulation, Ubl conjugation pathway |
Enzyme and pathway databases
Reactomei | R-MMU-112382, Formation of RNA Pol II elongation complex R-MMU-1234176, Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha R-MMU-674695, RNA Polymerase II Pre-transcription Events R-MMU-6796648, TP53 Regulates Transcription of DNA Repair Genes R-MMU-75955, RNA Polymerase II Transcription Elongation R-MMU-8951664, Neddylation R-MMU-9705462, Inactivation of CSF3 (G-CSF) signaling R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: Elongin-CShort name: EloC Alternative name(s): Elongin 15 kDa subunit RNA polymerase II transcription factor SIII subunit C SIII p15 Stromal membrane-associated protein SMAP1B homolog Transcription elongation factor B polypeptide 1 |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1915173, Eloc |
VEuPathDBi | HostDB:ENSMUSG00000079658 |
Subcellular locationi
Nucleus
- Nucleus Curated
Cytosol
- cytosol Source: Reactome
Nucleus
- elongin complex Source: UniProtKB
Other locations
- Cul5-RING ubiquitin ligase complex Source: UniProtKB
- VCB complex Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000187259 | 1 – 112 | Elongin-CAdd BLAST | 112 |
Post-translational modificationi
Keywords - PTMi
Ubl conjugationProteomic databases
EPDi | P83940 |
jPOSTi | P83940 |
MaxQBi | P83940 |
PaxDbi | P83940 |
PeptideAtlasi | P83940 |
PRIDEi | P83940 |
ProteomicsDBi | 275452 |
PTM databases
iPTMneti | P83940 |
PhosphoSitePlusi | P83940 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000079658, Expressed in primary oocyte and 329 other tissues |
Genevisiblei | P83940, MM |
Interactioni
Subunit structurei
Heterotrimer of an A (ELOA, ELOA2 or ELOA3P), ELOB and ELOC subunit (By similarity). The elongin BC complex interacts with EPOP; leading to recruit the elongin BC complex to Polycomb group (PcG) target genes, thereby restricting excessive activity of the PRC2/EED-EZH2 complex (PubMed:27863225, PubMed:27863226). Part of E3 ubiquitin ligase complexes with CUL5 or CUL2, RBX1 and a substrate adapter protein that can be either ASB2, KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B, FEM1C, SOCS1, SOCS5, ELOA, VHL or WSB1 (PubMed:10051596, PubMed:11384984, PubMed:9869640). The elongin BC complex is part of a complex with VHL and hydroxylated HIF1A.
Part of an E3 ubiquitin-protein ligase complex including ZYG11B, CUL2 and Elongin BC.
Part of an E3 ubiquitin-protein ligase complex including ZER1, CUL2 and Elongin BC.
Interacts with VHL.
Interacts with TMF1.
Interacts with SPSB1.
Interacts with SPSB1.
Interacts with KLHDC10; which may be an E3 ubiquitin ligase complex substrate recognition component.
Interacts with NOS2 in the presence of SPSB1 or SPSB2 or SPSB4 (By similarity).
By similarity6 PublicationsGO - Molecular functioni
- transcription corepressor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 212537, 39 interactors |
CORUMi | P83940 |
DIPi | DIP-42814N |
IntActi | P83940, 19 interactors |
MINTi | P83940 |
STRINGi | 10090.ENSMUSP00000140422 |
Miscellaneous databases
RNActi | P83940, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P83940 |
BMRBi | P83940 |
SMRi | P83940 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P83940 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3473, Eukaryota |
GeneTreei | ENSGT00390000011717 |
HOGENOMi | CLU_130038_0_2_1 |
InParanoidi | P83940 |
OMAi | VCEYLYY |
PhylomeDBi | P83940 |
TreeFami | TF300233 |
Family and domain databases
Gene3Di | 3.30.710.10, 1 hit |
IDEALi | IID50219 |
InterProi | View protein in InterPro IPR039948, ELC1 IPR001232, SKP1-like IPR011333, SKP1/BTB/POZ_sf IPR016073, Skp1_comp_POZ |
PANTHERi | PTHR20648, PTHR20648, 1 hit |
Pfami | View protein in Pfam PF03931, Skp1_POZ, 1 hit |
SMARTi | View protein in SMART SM00512, Skp1, 1 hit |
SUPFAMi | SSF54695, SSF54695, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MDGEEKTYGG CEGPDAMYVK LISSDGHEFI VKREHALTSG TIKAMLSGPG
60 70 80 90 100
QFAENETNEV NFREIPSHVL SKVCMYFTYK VRYTNSSTEI PEFPIAPEIA
110
LELLMAANFL DC
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A087WNT1 | A0A087WNT1_MOUSE | Elongin-C | Eloc Tceb1 | 134 | Annotation score: | ||
A0A087WQE6 | A0A087WQE6_MOUSE | Elongin-C | Eloc Tceb1 | 95 | Annotation score: | ||
A0A087WPE4 | A0A087WPE4_MOUSE | Elongin-C | Eloc Tceb1 | 51 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK013089 mRNA Translation: BAB28641.1 AK011757 mRNA Translation: BAB27825.1 AK011958 mRNA Translation: BAB27939.1 AK012909 mRNA Translation: BAB28546.1 AK039746 mRNA Translation: BAC30437.1 BC009104 mRNA Translation: AAH09104.1 BC028545 mRNA Translation: AAH28545.1 |
CCDSi | CCDS35517.1 |
RefSeqi | NP_001297399.1, NM_001310470.1 NP_080732.1, NM_026456.4 XP_006495624.1, XM_006495561.2 XP_006495625.1, XM_006495562.1 XP_006495626.1, XM_006495563.3 XP_006495627.1, XM_006495564.1 |
Genome annotation databases
Ensembli | ENSMUST00000115352; ENSMUSP00000111009; ENSMUSG00000079658 ENSMUST00000185771; ENSMUSP00000139675; ENSMUSG00000079658 ENSMUST00000186948; ENSMUSP00000140962; ENSMUSG00000079658 ENSMUST00000188641; ENSMUSP00000140422; ENSMUSG00000079658 |
GeneIDi | 67923 |
KEGGi | mmu:67923 |
UCSCi | uc007ajv.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK013089 mRNA Translation: BAB28641.1 AK011757 mRNA Translation: BAB27825.1 AK011958 mRNA Translation: BAB27939.1 AK012909 mRNA Translation: BAB28546.1 AK039746 mRNA Translation: BAC30437.1 BC009104 mRNA Translation: AAH09104.1 BC028545 mRNA Translation: AAH28545.1 |
CCDSi | CCDS35517.1 |
RefSeqi | NP_001297399.1, NM_001310470.1 NP_080732.1, NM_026456.4 XP_006495624.1, XM_006495561.2 XP_006495625.1, XM_006495562.1 XP_006495626.1, XM_006495563.3 XP_006495627.1, XM_006495564.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2FNJ | X-ray | 1.80 | C | 17-112 | [»] | |
2JZ3 | NMR | - | C | 17-112 | [»] | |
4JGH | X-ray | 3.00 | C | 17-112 | [»] | |
AlphaFoldDBi | P83940 | |||||
BMRBi | P83940 | |||||
SMRi | P83940 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 212537, 39 interactors |
CORUMi | P83940 |
DIPi | DIP-42814N |
IntActi | P83940, 19 interactors |
MINTi | P83940 |
STRINGi | 10090.ENSMUSP00000140422 |
PTM databases
iPTMneti | P83940 |
PhosphoSitePlusi | P83940 |
Proteomic databases
EPDi | P83940 |
jPOSTi | P83940 |
MaxQBi | P83940 |
PaxDbi | P83940 |
PeptideAtlasi | P83940 |
PRIDEi | P83940 |
ProteomicsDBi | 275452 |
Protocols and materials databases
Antibodypediai | 12329, 256 antibodies from 29 providers |
DNASUi | 67923 |
Genome annotation databases
Organism-specific databases
CTDi | 6921 |
MGIi | MGI:1915173, Eloc |
VEuPathDBi | HostDB:ENSMUSG00000079658 |
Phylogenomic databases
eggNOGi | KOG3473, Eukaryota |
GeneTreei | ENSGT00390000011717 |
HOGENOMi | CLU_130038_0_2_1 |
InParanoidi | P83940 |
OMAi | VCEYLYY |
PhylomeDBi | P83940 |
TreeFami | TF300233 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Reactomei | R-MMU-112382, Formation of RNA Pol II elongation complex R-MMU-1234176, Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha R-MMU-674695, RNA Polymerase II Pre-transcription Events R-MMU-6796648, TP53 Regulates Transcription of DNA Repair Genes R-MMU-75955, RNA Polymerase II Transcription Elongation R-MMU-8951664, Neddylation R-MMU-9705462, Inactivation of CSF3 (G-CSF) signaling R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 67923, 26 hits in 65 CRISPR screens |
ChiTaRSi | Tceb1, mouse |
EvolutionaryTracei | P83940 |
PROi | PR:P83940 |
RNActi | P83940, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000079658, Expressed in primary oocyte and 329 other tissues |
Genevisiblei | P83940, MM |
Family and domain databases
Gene3Di | 3.30.710.10, 1 hit |
IDEALi | IID50219 |
InterProi | View protein in InterPro IPR039948, ELC1 IPR001232, SKP1-like IPR011333, SKP1/BTB/POZ_sf IPR016073, Skp1_comp_POZ |
PANTHERi | PTHR20648, PTHR20648, 1 hit |
Pfami | View protein in Pfam PF03931, Skp1_POZ, 1 hit |
SMARTi | View protein in SMART SM00512, Skp1, 1 hit |
SUPFAMi | SSF54695, SSF54695, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ELOC_MOUSE | |
Accessioni | P83940Primary (citable) accession number: P83940 Secondary accession number(s): Q63182 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2004 |
Last sequence update: | June 7, 2004 | |
Last modified: | May 25, 2022 | |
This is version 157 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families